LOCUS       c00133_gnlPro..         5375 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FJCFCKGF_133.
ACCESSION   c00133_gnlPro..
VERSION     c00133_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:21:21
            Original ID  :: gnl|Prokka|FJCFCKGF_133
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5375
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5375
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3069:5373]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3070..5373
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5375
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5375
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(170..1402)
                     /locus_tag="ctg133_1"
                     /transl_table=11
                     /translation="MKILLVTRGSQGDVLPYLAIAAELERRGHEVTINLPQIFEETVKP
                     YGFKYVLQQFDDIGGMIDSAAQNSHKFRPFLKWMRNVIDKQFDQLIPLLKEHDILVSTN
                     SEFAVASIAEYCKKPLIRTAYAPFLPGKKIPPAVLPFPKPNPIITPAILWKLMNRMTNF
                     MVKDTINKNRAKYGLAPIRNFGYHAGERSYNYLLFSQHLGNIDPDWTFKWSIGGYCFND
                     TFQYDEKAYEEMISFVDSAQSPIIFFTLGSCSSKDGNRFSKALVDICKKHDYRLIIDSG
                     WAKTGITLQADKHLFLMKQPVPHNLIFPHCDGVIHHGGCGTTHSVGRAGKPQLITPLII
                     DQPYWSYRIHQLGLGPEGLKIAKASEQEIERKVCDLVTNPLYKKNASHIGEMIQKEGGI
                     KNICDSIERFQ"
     CDS             complement(1436..2647)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.2e-17, bitscore:
                     51.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPDRIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQIEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
     CDS             2923..3033
                     /locus_tag="ctg133_3"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             complement(3070..3825)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_4"
                     /sec_met_domain="Fer2_3 (E-value: 5.6e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.1e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(3856..5373)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 119; E-value:
                     9.5e-37)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 7.6e-38, bitscore:
                     119.0, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.7e-39,
                     bitscore: 122.6, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRQIGLGKVKMY
                     TRHEMLDVVKVDGRARGIIARNLITGKIERYAAHAVVVATGGYVNTFFLSTNAMASNGS
                     AAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKLTLMSESLRNDGRIWVPKKLEDAKA
                     LQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNSTGLAVFL
                     DFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYETPMMIYPALHYSMGGLWVDYELMT
                     SIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQVPRFSTDLP
                     EFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHIMWEHIGMARDAKGLQEAIQMLKDL
                     KKEFWSNVFIPGNADNLNTELEKALRVADFIEIGTLMAHDALDRAESCGGHFRTEHQTE
                     EGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYEFVVPQTRNYKK"
ORIGIN
        1 attaaagcct gtctcacgac aagccctaac caaactttgt taaccttaaa tctaatacta
       61 ttatgaaaaa accacgatgc aaatatacgg agttactatt atttgtcaat agtttaagtg
      121 ctaaattgtg ttttgtaaca tgtaataaaa tctaatagga acttatgcct cattgaaatc
      181 tttcgataga gtcgcatata ttctttatac ctccctcttt ttggatcatc tcgcctatat
      241 gagaggcatt tttcttataa agcggattgg tgaccagatc acaaaccttg cgttcgatct
      301 cctgctcgct cgctttcgct atcttaagcc cttccggccc taaccctaat tggtggattc
      361 gataggacca atagggttga tcgataatca acggagtgat taattggggc tttcccgccc
      421 ttcctacgct atgggtcgtg ccgcaacctc catgatggat aacgccgtcg cagtgcggaa
      481 atatcaaatt atgcggaacc ggctgcttca ttaagaataa atgtttatcc gcttgtaagg
      541 tgattcctgt ctttgcccaa ccggaatcga tgatgagccg ataatcatgt ttcttgcaaa
      601 tatcgaccaa ggctttgcta aaacggttac catccttgga actacaactc ccgagggtaa
      661 aaaagataat agggctttga gcgctgtcga cgaatgatat catttcctcg taagccttct
      721 catcatattg aaaagtgtca ttgaagcaat agcctccgat cgaccatttg aacgtccagt
      781 ccggatctat gtttcctagg tgctggctga aaagcaagta attatatgaa cgctctcccg
      841 catgataacc aaaattacga atcggtgcga gtccgtattt agcccggttc ttgttgatcg
      901 tgtctttgac catgaaattg gtcatccggt tcatcaactt ccataagatg gcaggggtga
      961 tgatcgggtt gggtttggga aatggcaata cggccggggg aatcttctta cccggcaaga
     1021 acggggcgta ggcggtacgt atcaagggct tcttgcaata ctcggcgata cttgcgacag
     1081 caaattccga gttggtcgat actaaaatat catgctcttt tagtagggga atcaattgat
     1141 cgaactgttt atcgatcacg ttcctcatcc atttaaggaa tggccggaat ttatgtgagt
     1201 tttgcgctgc gctatcgatc atgccgccga tatcgtcaaa ctgctgcaat acgtatttaa
     1261 atccgtaggg tttaacggtc tcctcgaata tttgagggag attgatggtc acctcatggc
     1321 cacgtctttc caattcggcc gctatggcca aataaggaag gacgtcgcct tgtgagcctc
     1381 tggttactaa tagtattttc atgctacgtt tatttatggg ctatgagggt cttgtttacc
     1441 ggatattcag ttgacgcaaa cgctcgatgt ccagatggta tttggcttcc atcgtttgta
     1501 gtttctgctc attgatggct aggggatgat tcaatcgttt cgtttcccgg tatcctttat
     1561 atacggagcg aatcatctta cgtggattga aaatgacgga tgaggtgatc cagcagccgt
     1621 gcgtacacca acagttggct ttatacccat gtccgatagc ggctatttcc tgtttcatcg
     1681 cttcgctatt catcacttta cttatatcgc aaccatactc ttttatattt ccgagcggtt
     1741 cccgtagctc gcaggcacga aaacgtccgt cgtagtcgat taccaaggta gtctctccgg
     1801 ccgtacaatc catcccccaa ggggtcgggt gatctatgtt gctggcacgc acattatata
     1861 aggtacgtat aaggccgaga tagaagaagc gtgtgatcgg tttccttaag ccggtggtat
     1921 ccgctaccat ccgatccgca taagcagaat agatagggga tatgtcatct tgtattttgc
     1981 gtagcgttga ctcggttagg attttcacgc catcgtcacg ggtcatgcct ctggcagcct
     2041 caatcgtatg cgtggaggta cggaaacgtc cgtataccca agtcatgaag tcttctatct
     2101 ggtctatatt gtatttcgtg ataacggttg ctatattttt taagactttg ccgtcatctc
     2161 cgaaattctc ctgtatctta cgcagggttt ccaaggcctt atagaagttt cccggtacgc
     2221 ctctctgtgt atcgtgggtc gtaccgaagc catccaagga aaggcttacg ttaatattac
     2281 aatccgggca gttctggcgg aaccgtttta cccattcgac gatccggtct ggttttaagc
     2341 cgttcgtagg gatgttcaca tctttgatat ggttgttttt gtagaataac tccaatattt
     2401 ccggtagatc ctcacgcaag gtaggctcac ccccggatat ccataatcga ttgaattgcc
     2461 cggcggtttc tgatatcttc ttgatttcat cgaaagtgag gtcgggttgc ttcttatcca
     2521 tttcttgggc atagaaacac attgcgcatt tggcgttgca tcggcctgtt acgaaaagga
     2581 agatcgactc gagttttctt tccgtgctca ttgccctgaa agaactggtg gttcgttttt
     2641 tgtccatttt atttttgttt taccatttgt cttatctggc gcatgatttt ttccttgtgt
     2701 ttgatcaaca cccggagaaa ataacataag aagccaaata aaatacttat tagcatatac
     2761 tatatgatct ttaaaggcaa agatcgacga taaaatgacg ctaaacaatc tctatttgca
     2821 gtattttatg gtccatgttt aaaaggtagg tctataaaca attaaagcct gtctcacgac
     2881 aagccctaac caaactttgt taaccttaaa tctaatacta ttatgaaaaa accacgatgc
     2941 aaatatacgg acttttcaat tcctgtcaag ggttataatg ctaaattatg ttttacgaca
     3001 tataacaaaa acaaaaaggc gcctttcttt tagagaaaga cgccttttcc atgatatact
     3061 aatcaaatat tagtcttgta atttagcgca aatgaactcg cggttcaaac gagcgatgtt
     3121 gctaacggag atattcttcg gacattccat ctcgcaagca cgtgtgttcg tacagttacc
     3181 gaagccaagc tcatccattt tagcgaccat agctttggca cggcgagcag cctcgacttt
     3241 accttgaggc aacaaagcca gctggctaac cttagcggat acgaataaca tagcggaacc
     3301 attcttacaa gcggcagcac aagcgccaca accgatacaa gcggcagcgt ccatggctaa
     3361 gtctgcgtcc ttctttggga taggtatcgc attagcgtcg ggtacaccac ctgtgttgac
     3421 agatgtgaaa ccacccgctt ggatgatttt gtcgtaagcg tagcggtcta ccatcaagtc
     3481 gcggataaca gggaaacctg cggaacgcca cggttcgatc gtgatcgtct ctccatcgtg
     3541 gaaacgacgc atatataact ggcaagtcgt agctccagtt gccggtccgt gcgggtgtcc
     3601 gtttacatac aatgaacaca tgccacagat accctcgcgg cagtcgtgat cgaatacgat
     3661 cggttcctta ccctcgttga ccaactgctc gttcaagata tccaacattt ccaagaaaga
     3721 gacactctga ttgatcttct caattttgta tgtctcgaac tgtcctttct ccttcggacc
     3781 tctctgacgc caaaccttaa gtgttacact tatatcttta tccatgattc taattttttc
     3841 tgagattgat gattattact tcttgtagtt acgtgtttga ggaacaacga actcatagtt
     3901 caaatcctct ttcaacatga caggatcttt gtcctcgcct tggtactccc agcaagatac
     3961 gtacatgaac ttatcgtcat gacgcaaggc ctcaccttct tctgtctggt gctccgtacg
     4021 gaagtgacca ccacaagact cagcgcggtc caaagcatcg tgtgccatca atgtaccgat
     4081 ctcgataaag tcggctacac gtaatgcttt ctctaactcg gtattcaagt tgtctgcgtt
     4141 gcctgggatg aatacgttgc tccagaactc tttcttcaag tctttcagca tctggatagc
     4201 ttcttgcaaa ccttttgcgt cgcgtgccat accgatatgt tcccacatga tatgacctag
     4261 tttgcggtgg atagtgtcta cggtttcttt acccttgacg ttcatcaatt tttggatacg
     4321 atccttgatg gccttttctg cctcgacgaa ctccggcaga tccgtgctga agcgaggaac
     4381 ttggatttgg tcggacaaat agttctggat cgtgtatggt aatacgaaat aaccatcggc
     4441 caatccctgc atcaaagcgg aagcacctaa acggttcgct ccgtgatcgg agaagtttgc
     4501 ctcaccgata gcgaacagac ccggaatgga ggtcatcaac tcatagtcaa cccacaatcc
     4561 acccattgag tagtgcaatg ccgggtagat catcatcgga gtctcgtatg gattatcgtt
     4621 cgtgatctct tcgtacatat cgaacaagtt accgtatttc tgcttaacga cctctttacc
     4681 caagcgattg atcgcgtcgg agaaatccaa gaatacggcc aagcccgtgc tgttcacgcc
     4741 gaaacctgcg tcgcaacgct ctttagcggc acgagaagcc acgtcgcgag gaaccaagtt
     4801 tccgaatgcc ggataacgac gctccaaata ataatcacgg tctgcctcgg gaatatcttt
     4861 acctttcttc gttccggcct gtagagcttt agcgtcttcc agtttcttcg gaacccagat
     4921 acggccgtcg ttacgcaagg attcggacat caaagtcaat ttagattgga agtcaccttt
     4981 caccggtacg caagtcgggt ggatctgaac catacaagga ttagcgaagt aagcgccttt
     5041 cttgtagcat tgccatgcgg cggaaccgtt agatgccata gcgttggttg acaagaagaa
     5101 cgtattaacg taaccacccg tagctacaac tacagcgtga gcggcgtaac gctcgatctt
     5161 acctgtaatc aagttgcggg cgatgatacc acgagcacga ccgtctacct taactacatc
     5221 caacatctca tgacgagtgt acatcttaac cttacccaaa ccgatctgac ggctcaacgc
     5281 tgaataagca cctaacaaaa gctgctggcc cgtctggcca cgggcgtaga acgtacgcga
     5341 tacctgagcg ccaccgaatg aacggttatc caaca
//
