LOCUS       gnlProkkaOLLNFHLF_33    5527 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OLLNFHLF_33.
ACCESSION   gnlProkkaOLLNFHLF_33
VERSION     gnlProkkaOLLNFHLF_33
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 06:25:37
            Original ID  :: gnl|Prokka|OLLNFHLF_33
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5527
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5527
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1284:5525]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1285..5525
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5527
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5527
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             3..1271
                     /locus_tag="ctg33_1"
                     /transl_table=11
                     /translation="MSRSPLYVLALYASVLIFLVYTCAYAYRKPFTAAIYEGEILWGFD
                     VKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALLGFALLPVPLKV
                     CSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTLGQFVMDNLHVS
                     EYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDRRKFLRQFFWGI
                     CMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFVLVLMFLIVFVRN
                     NRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGYIPFTYLIERLI
                     ASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTALFSGFVSVLSI
                     FAIYCYFKQQLNSLILIPSEIHD"
     CDS             1285..1983
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_2"
                     /sec_met_domain="NTP_transf_3 (E-value: 5.7e-15, bitscore:
                     44.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             2003..3304
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_3"
                     /sec_met_domain="CTP_transf_like (E-value: 1.3e-13,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.6e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.3e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             3316..4452
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 70.6;
                     E-value: 1.9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_4"
                     /sec_met_domain="TPP_enzyme_N (E-value: 7.5e-23, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.5e-21, bitscore:
                     64.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGIALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             4449..5525
                     /NRPS_PKS="Domain: Aminotran_5 (30-293). E-value: 5.9e-34.
                     Score: 109.4. Matches aSDomain:
                     nrpspksdomains_ctg33_5_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.6; E-value:
                     1.8e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_5"
                     /sec_met_domain="Aminotran_5 (E-value: 3.7e-32, bitscore:
                     100.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVM"
     aSDomain        4539..5327
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg33_5_Aminotran_5.1"
                     /evalue="5.90E-34"
                     /label="ctg33_5_Aminotran_5.1"
                     /locus_tag="ctg33_5"
                     /protein_end="293"
                     /protein_start="30"
                     /score="109.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIK"
ORIGIN
        1 ttctttcccg ttctcccctt tacgttttgg ctctctatgc cagcgttttg atttttctgg
       61 tttatacttg tgcctatgcc taccgtaagc catttaccgc agccatttat gaaggtgaga
      121 ttctttgggg attcgatgtc aaaatcctgt atgtcctttc tgagattatc ggctacgccc
      181 tttccaagtt catcggtgtt cgtattctgc cttcgatgaa agcgggtcac cgcatctatt
      241 atataatagg gctgcttact ttttccgaag ttgctttgtt gggttttgca cttcttccag
      301 ttcctttgaa ggtgtgttct atttttctga gcggtcttcc cctgggtatg atctggggtg
      361 tcatttttag ctatatcgaa gggcgtcgta tcagtgaaat attgaatgtc gggctgagcg
      421 ttgctttgat agtctcttcc ggtttggtga aaaccttggg acaattcgtg atggataatt
      481 tgcatgtgtc ggaatattgg atgcctttta ccaccggttt gttggtatac cctgttatgt
      541 tgctttgcag ctggcttctc aatcagattc cggctcccaa tgagcaagat atcatacagc
      601 gtaccaaacg tgctccgatg gatggtaaag acaggcgtaa atttttgagg caattctttt
      661 ggggaatctg catgctggtt gttttttacg gtgccctgac cgtgttccgt gagcttcgtg
      721 actctttcgc tgccgatatt tggaaggagc tccatattga gggtgcaatg atctttactc
      781 agacagaggt tcccattgcc gtttttgtcc ttgtactgat gttcctgatt gtgtttgtcc
      841 gtaataaccg tttggctctt aatatcattt attgcatcgc cgtcacaggc ggttccttaa
      901 tggttttttc cacattgctt tatgtgtacg gttttctttc tcctatttgg tggatgattc
      961 tttccggtct tggtctgtat atggggtata ttcctttcac ttacctgata gaacgtctga
     1021 ttgcttccct tagggtggtt agtacggctg tttttattat ttatttggcc gattctttcg
     1081 gctatctggg cactacgggt gtgtttatgg tcaagaactt cgtttctgct gacatttcct
     1141 ggactgtcat gctgatgcgt actgcccttt tttcaggatt tgtttctgtc ctgtcgattt
     1201 ttgcgattta ctgttatttc aagcaacaat tgaattcact tatattaatt ccctctgaaa
     1261 ttcatgatta aaacagcaat gatcatggct gccggtcttg gtacccgctt cgggcattat
     1321 accgagcttg tgcccaaagg ttttgtggag gttggtggaa aacctatgat tatccgctct
     1381 atcgaaaccc ttctttcctg cggtatcgaa cgtatcatcc tcggtacggg ttataaaaaa
     1441 gaggcttatg aagcccttca ggcggatttc ccccagatag agacctgttt cagtccccgt
     1501 tatgccgata ctaacagtat gtacactctg tacaatacgc gtgacgtgat aggcgatgac
     1561 agtttcctgc tgcttgaatc ggaccttgtt tttgaacgca aggccatcct ctcgctgctg
     1621 gatgacgaat tcccggatgt gatgcttgtc tcttctctga ccaagttcca ggatcagtat
     1681 tatgtggaat atgaccgcaa tcacatcctt acttcctgtt cggtagacaa ggacgccctt
     1741 gaggcaaaag gcgagctggt cggtatccat aaactttcca atactttcta ccgccggatg
     1801 tgcgccgatt atgcgaccat cctggagtcc cagcctaaac tgggttacga gtacgaactg
     1861 ctccgcatgt cccgttccgt ttcccctgtc cgtgtgctca gggtggaggg tcttaaatgg
     1921 tatgaaatcg atgatgaagc cgacctttcg tatgctgagg agcacatcat ccgctattgt
     1981 taaaaacaaa tcttttccat atatgaacaa gaaaatctat gtcggtatga ccgcggatat
     2041 catgcatccg gggttgatcc atattattca tgaagctact aaatacggtg atgtcatcgt
     2101 cggtctgctg acggacaagg ccatcgccga acacaaacgc ctcccttacc ttacctatga
     2161 tcagcgtaag gaggttgttg agaatatcaa aggggtttgc gaagttgttc cccaggagga
     2221 ttggagctat gttccgaatc tggaaaaact caagcctgac tatatcatcc acggcgacga
     2281 ctggaaaacg ggcccgttgc gcgaggtgcg cgaacaggtt tttgaggtga tgaacgccca
     2341 ggggggcaag gtaatcgaga tcccttatac ccggggcatc aactcctcct cgctcgacaa
     2401 ggatatcaag gcgatcggga ctacccctga cgtacgtctt aagtccctcc gccgcctgat
     2461 ccaggccaag cctgtggtcc gtatcctgga ggctcacgac ggtctttgcg gtctgattat
     2521 tgagaatctt gaagtccaga agggtgataa gcgcgaggtc tttgacggca tgtggtccag
     2581 cagcctgacc gactctacga gtaagggtaa accggatatt gaagcggtcg accttaccac
     2641 ccgtttacag gacctgaaca atatccttga atgtaccacc aagcctatta tttttgatgg
     2701 agacacgggt ggcaagatcg aacactttgt ctttactgtg cgcactctgg aacgtcacgg
     2761 catttctgcg gtaatcattg aagataaagt cggtctgaag aagaattccc tttttggtac
     2821 tgacgctatc cagactcagg atagtatcga gggtttctgc gataagatca gagcagggaa
     2881 gaatgcccag gtcaccggtg actttatgat cattgcgcgt atcgaaagcc ttattgccgg
     2941 caaacccatg agtgacgccc tcgaacgtgc ttatgcctat gtacaggcgg gtgcggacgg
     3001 catcatgatc cacagcaagg ataagtcggg tgaggatatc cgtgagttct gccggacttt
     3061 ccgcaaagaa tacgcccatg ttcccattgt cgttgttccg acgacttatg accatgtgca
     3121 cgaatccgaa cttcacgaat ggggtgccaa cgtagtcatt tacgccaacc accttctccg
     3181 tgccgcttac ccggccatga tgaacgtggc caggaccatc cttgaaaacg agcgcgccga
     3241 ggaggtccgt ccgctttgta tgcctatcaa ggaaattctg gaattgattc cgggcactaa
     3301 ataaatcaga cggttatggt aagtgtagaa aagatatacg atacgttcct tcatcacggt
     3361 gttgacttct ttacgggtgt tcccgattcc cttttgaaga atatttgcgc ctatatcacg
     3421 gatcatgctc cgcgtggtaa gcatatcatc gccgccaatg aaggttccgc ggtgggcatt
     3481 gcctgcggtt attatatggc ttctggcaac gttcctttgg tttatatgca gaactccggt
     3541 ctgggaaata cggtgaatcc cctcttgtcc cttgcggacg agaaagtgta cagccttcct
     3601 cttcttttgc tggtcggctg gcgcggtgag cccggcacga aagacgaacc ccagcataag
     3661 aagcagggcg aggttaccct tgatcttctg aaagccatga aaattccttt tataattctg
     3721 gatacggatg aagacgaggc cctggcgcag atccatgatg cggttgtctc ggcgaagagc
     3781 cggtcgattc cccatgcgat tgtcatccgt aaagatacgt ttggaaaata taaattgcgt
     3841 caggaagcct tgaatgacta tcctctgtcc cgcgaggagg cgttaatgca ggttgtcgac
     3901 cacctcgggg acaatgacat tgtggtttcc accacgggca agctctcccg tgagcttttc
     3961 gagtaccgtg aatcgaaagg gcaggggcac gcaagggatt tcctaactgt gggttccatg
     4021 gggcacagtt cttccattgc cctggggatt gcccttgaga agcctgaccg ccgtgtctac
     4081 tgtcttgacg gtgacggtgc ctttatcatg cacatgggtg ctgtcagcaa tatcggcgat
     4141 ctttctccca agaattacag gcacgtcctt ttcaacaacg gtgcgcatga atctgtcggc
     4201 ggtcaaccca cgctcggttt ccgtctggat atcgcctcga tagcgaaagg ttgcggttat
     4261 gcgcatgtac tgactgcctc cgacaaggaa gggctttcgt gtgcgcttga gaagctgtcc
     4321 ggcctgtccg gcccggttct tcttgaaatt aaggtccgga tcgattcacg tgatgatctg
     4381 ggtcgcccta cgactactcc ggtagagaat aaagagcatt ttatggcatt tttaaaagat
     4441 acgcattcat gaatatcgaa agaaagatcc ttttgaatcc gggtcccgcc accacgacgg
     4501 atacggtgaa gctggcgcag gttgttcccg atatctgtcc gcgtgagaaa gagtttgcgg
     4561 gtatgatgaa acagttacgt gacgacctgg tccgcgttgc tcatggagac ctctccaaac
     4621 atacggccgt cctgttttgc ggctcgggta cgatcaatat cgatatctgc ctgaactctc
     4681 ttcttcctgc ggataagaaa gttctggttg tcaataacgg tgcctacagc acccgtgcgg
     4741 tggaagtctg cgaatattac ggtcttcccc atatcgattt gaagttttcc gtttacgaac
     4801 ttcccgacct gtcggtaatc gaaaagacgc tttccgacaa tccggacatc gctttggtct
     4861 ataccacgca ccatgaaacg ggtacgggta tcttgaaccc catccgtgag atcggaaaaa
     4921 tagcccattc gcacggtgcc gtttttgttg tcgacaccac ttcctccctg ggtatgattc
     4981 cttttgatat cgggaaggat aatgttgact tttgtatggc ttcggctcaa aaaggtcttc
     5041 aggccatgac gggtctttcc tttatcatag gtgatgaatc cctgatacgt gcttcaaggg
     5101 attatccaaa acgttcctac tattgtaacc tctatttgca atacgattac tttgagaaga
     5161 cgggtgagat gcattttact cctcctgttc aaaccgttta cgctgcccgt caggctcttg
     5221 atgaatattt tgcagtaggt gaagaggcta aattcgcccg tcacaagagg gtctttgaag
     5281 ccatccataa aggtttgcaa gagcttggtc ttcagtatgc gatcaaacgt gaatggcagt
     5341 cgggtcttgt tgtttctgta ttgtatcctg atgatcccct ctgggatttt gataaggttc
     5401 atgattactg ctatgaacgt ggctttacga tctacccggg taagatttcc acaacgaata
     5461 cattccggct ttgtgcgttg ggagagattg atgtggagga tattgagagg ttctttgagg
     5521 tgatgag
//
