LOCUS       c00143_gnlPro..         5191 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HAPJPOOJ_143.
ACCESSION   c00143_gnlPro..
VERSION     c00143_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:47:21
            Original ID  :: gnl|Prokka|HAPJPOOJ_143
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5191
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5191
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2936:5190]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2937..5190
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5191
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5191
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(216..1781)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg143_1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.3e-49, bitscore:
                     156.1, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSAMKDQLSGIFSGLVSQYVFDIQVAPDHESRQELIDLLGDVA
                     SCSDHLSCSVADGEGLQFTIVKDGMPTGIRFRAVPNGHEFTSLLLAVLNCDGKGKNIPD
                     EGICARVRALNGPIKLTTYVSLTCTNCPDVVQALNVMATLNSCITHETVDGAINQKEVE
                     AMKVQGVPSVFADGRLINVGRSDLGELLSKLEAQYGINEGGVDNSVEKPVKNYDVLIVG
                     GGPAGAASAIYSARKGLNVAVIAERIGGQVKETVGIENLISVPQTTGKQLANDLLTHIN
                     DYPVDILEHRRVDKVELDGSAKFLTTSTGERFSAPALIVATGASWRKLNVPGEADYIGK
                     GVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEK
                     AKSLPNVEVFLHSQSLEVLGNGDKVTGLRVKDRKTDAERVIDLDGIFVQIGLAANSGVF
                     RDVVETNKPGEIVIDAHCRTNVPGIYAAGDVSTVPYKQIIIAMGEGAKAALSAFEDRMR
                     GSIG"
     CDS             complement(1786..2352)
                     /locus_tag="ctg143_2"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     NEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             2629..2934
                     /locus_tag="ctg143_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCKGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             complement(2937..3659)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg143_4"
                     /sec_met_domain="Radical_SAM (E-value: 1.8e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(3676..5190)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg143_5"
                     /sec_met_domain="Gly_radical (E-value: 4.3e-47, bitscore:
                     147.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.6e-182, bitscore:
                     596.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGK
                     LTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSLVTRTSY
                     RYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDY
                     GIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITSEYLDYN
                     EVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGIAGLSIV
                     ADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFMEYLRQH
                     QTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGAVAALAS
                     VAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNVNVFDRD
                     LLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
ORIGIN
        1 cttgaatatt aatagctaca accttcgtag tgatagagtg atagaggatt tgtagaaact
       61 tcgtatggag atcacaacct tggaaaattt tgatgcggtt gccctgttgt gaaagtcggg
      121 gctttgtgcg taaaaaagaa aaggtgtgca attttgaaca cacattcgtg tagttcaatt
      181 tgcacacctt cgtcgttcat agcaaatact aaatattaac cgattgaacc tctcatgcgg
      241 tcttcaaaag cagatagagc cgcttttgcc ccttctccca ttgctatgat tatctgttta
      301 tagggtacgg tcgagacgtc gcccgccgca tagatgccgg gaacattcgt ccgacaatgc
      361 gcatcgatta caatctcacc cggtttgttc gtttcgacaa cgtcgcggaa aacgccacta
      421 ttggctgcca agccgatttg gacaaagatt ccgtccaggt cgatcacccg ctccgcatcc
      481 gtcttacggt cttttacacg aagacctgtc accttatcgc cattaccaag cacttccaaa
      541 gattgggaat gcaagaaaac ttccacattc ggcaagctct ttgctttttc ctgcaacact
      601 tggtcggctt tcagttcgtc cataaattcc agaaccgtca ctttcgaaca aatgcctgcc
      661 aaatctatag ccgcttcgat acccgaattt ccaccgccga caaccgctac gtgcttacct
      721 ttatagaacg gaccatcgca gtgagggcaa aaagcgacac cttttccgat atagtccgct
      781 tcgcccggaa cattcaactt gcgccagctt gcacctgtcg ccacgataag ggcaggagca
      841 gaaaaacgtt caccggtaga agtggtcaga aatttcgccg atccatccag ttcgacttta
      901 tccacacggc gatgctccag tatatcgacc ggatagtcgt tgatatgtgt caaaaggtcg
      961 ttcgccaact gctttccagt agtctgagga acggaaatca ggttctcgat accgaccgtt
     1021 tctttgacct gtcctccgat acgttcggct atcacagcaa cattcaagcc tttacgtgcc
     1081 gagtagatag cagaagctgc accggcaggt ccacctccca ctattaacac atcatagttc
     1141 ttaacaggtt tttcaacaga gttatcaaca cccccttcat taattccgta ttgggcttcg
     1201 agcttggaaa gcaattctcc caaatcacta cgccccacat tgatcagcct gccatctgca
     1261 aaaacagaag ggactccctg tactttcatg gcctcaactt ctttttggtt gatagcacca
     1321 tccaccgttt cgtgagtgat acagctgttg agagtagcca tgacattgag tgcctgcacc
     1381 acatccggac aattggtgca agtaagagac acataagtcg tcagcttgat cgggccgttc
     1441 aaagctctga cacgggcaca gataccttcg tccggtatat ttttaccttt tccatcgcaa
     1501 ttcaggaccg ctaacagcaa agaagtaaac tcatgtccgt taggtactgc acgaaaccgg
     1561 ataccggtag gcataccgtc ttttacaatt gtaaactgaa gtccttcgcc atccgcaacg
     1621 ctgcatgaca aatgatccga gcatgatgcc acatcaccaa gcaggtctat tagctcctga
     1681 cggctttcgt gatcgggagc gacttgtata tcaaatacat attgggatac cagtccggag
     1741 aaaatacccg aaagttggtc tttcattgct gagtctaaca taactttaga tcttaccaac
     1801 caagtcgata ctcggcttca aggttgcttc gccttttttc cactttgcag gacaaacttc
     1861 gttcgggtga gaagcaacga actgggcagc ttcgacttta cgaagcaatt cgtctgcatt
     1921 acggccgata ccgttatcgt ggatttcggc aatcttgatc tttccctcgg gatttaccag
     1981 gaacgtaccg cgataagcca tcccctcttc ttcaatcatc acaccgaacg cacggcttaa
     2041 agcccctgtc gggtctgcca gcatcggata tttgatctta cggatcgttt cggaagcatc
     2101 atgccatgct ttgtgtacaa agtgcgagtc tgtacttacg gaatatactt cgacacccat
     2161 ttcctgaaat ttgccgtact tgtcagccat atcgaccaat tcggtcggac atacgaacgt
     2221 aaagtcggca ggatagaaga aaaagatagc ccatttgccc ttgatatctt cgctacttac
     2281 tgtcttgaaa ctaccattct gaaaagcctg tactttgaat tcgggtactt gtgaattgat
     2341 aattggttcc atgattttta ctatttaatt tgttactact attttttgtt taccctacaa
     2401 aggtaaatcg taagatactg attgaaagaa tttttcaaat tgataatttt tatctgccga
     2461 tagacggatt tctatctgca agcatactta tctactatga caataggaag caaagtaatg
     2521 ttttccggaa caataaaaat atacctaatt gtaggtaaaa tacctttcga tcataccatg
     2581 tgattcttta attgttaact ttggatgtta acttaaaaat attcgaagat gagaagagct
     2641 agtttgtata atacattttc agcaagtcta ttgctcgttt gcaacgcatg tctttcttca
     2701 tgtgtctcac aaatacccct tacaaaggaa aaaccggtaa acaattccac ttatcaagtc
     2761 gaatatctgt tcgaacacga agggtgtaag gtctatcgtt tcatggatat gggacattac
     2821 atctatttca ccaactgcaa gggagatgtt accagtattg aaagcgacag cgtccgcaca
     2881 gtcaaccgga tcagccgtaa accgatcatc atcaaagagc agacactcga ttaggtttat
     2941 acggttaccc cgtgtttcat aaagatcgct ttcgcattgg ccagacgttc tgctgaaagc
     3001 ggttccacac ctttcagctt atagtccagg ccttgcgcct catatttgta agctcccatt
     3061 gtatgataag gcagaagttc ggctttctgg accacgttat atgaactgat atattcggcc
     3121 agcttgctca aggcttcatc atcgtccgtc aggccgggca cgatcacgtg gcgaatccat
     3181 acagggatgc cgcgtttctc cagttcatca agaaagcgta aagtattatc gagttttacg
     3241 gccgtcaaac ggggatgaag accggcgtcg atcgtcttga tatctaacag aaccagatcg
     3301 acctgttcga gcacctccaa tgctttaccg gaacagatat agccggaagt gtccaacgcc
     3361 gtatgaatac cgttctcacg gcaaaggcgg aaaaactctt taaggaattc aggttgtaac
     3421 aaaggctcgc caccggtcac ggtaacacca cctttagcga taaaattctt atagcgcagc
     3481 acttcggcaa gcaacgcttc aggttccagc agataaggcg tttcacgctt tacctcccag
     3541 gtatccggat tgtggcaata cagacaacga agcggacatc cctgcataaa aacgacaaag
     3601 cgaattccgg gaccgtcaac ggtcccgaaa ctctctaaag aatgtatacg ccccaacata
     3661 ttaatcaatg gacaattata aactgctgtt gatggtgcgc gagatgacgt caagctgctg
     3721 ttcgcgtgtc agcttcacga agttgacggc atagccagaa acgcggatcg taagctgcgg
     3781 gtatttctcc ggatgctcca ttgcatctaa caacaagtcc ctatcgaaca cgttcacatt
     3841 cagatgctgg ccaccgtcag gcgtaaaata gccatccaac aggctcacca aattatttat
     3901 ctgaatatcg cgctccttac ccaacgtagc cggtgaaacg gcaaatgtat aggaaatacc
     3961 gtcatgcgca tgctgaaaag gcagtttggc tacagaagcc aaagcggcga cagccccttt
     4021 cgtatcgcgt ccgttcatcg ggttagctcc cggagcgaac ggcgtaccac ccggacggcc
     4081 gtcgggagtc gttccggttt tctttccgta gaccacgttt gatgtgatcg tcaggataga
     4141 ttgtgtcgga gttgccttgc ggtatgtctg atgctggcgc agatattcca taaacttctc
     4201 agtaatgtcg acagcaatac tgtccgtgcg gtcgtcattg tttccgaaag gtacatattc
     4261 gccttcgcgt tcgaagtcga cagccaaacc acgttcgtca cggatcactt tcactttcgt
     4321 gtcacggatg gcagccagcg agtcggcaac gatagaaagg ccggcgatac cggttgcgcg
     4381 gatacgttcc acatctccgt catgaagccc catctcgaat gcttcatacg catatttgtc
     4441 gtgcatgtag tgaatgatct tcagagcatt gacataaact tttgccaacc agcgcatcat
     4501 ctgctcgtat ttctccatca cttcgttgta atccagatac tcgctggtga tcggttcgaa
     4561 acggggagcc acctgcacac ccgatttctc gtcacgtccg ccattgatcg catacaacaa
     4621 gcactttgcc aggttggcac gagctccgaa atactgcatc tgcttaccga tcttcatagg
     4681 agaaacgcaa caagcgatac cataatcatc tccataatcc ggacgcatca aatcgtcgtt
     4741 ctcatattgg atagccgacg tatcgataga gaccttggca cagaaacgtt tccagttctc
     4801 gggagcgttc ttaaaccaca gaaccgtcaa attgggttct ggtgccggac ctagattata
     4861 taatgtatgg agataacgat aggaagttct tgttaccaaa gaacggccat ccactccttg
     4921 gccgcccaag gactctgtta cccaaaccgg atcaccggag aacaaatcat tgtattccgg
     4981 cgtacgtaag aaacgaacga tacgcaactt gatgatcaac tggtctacca attcttgcgc
     5041 ctcctcttcg gtcaacttac cctcacggat gtctttctcg atataaatat ccaaaaacgt
     5101 agaagtacgt ccgatagaca tcgccgcgcc atcttggtct tttaccgccg ccagataagc
     5161 taagtacaca aactgaactg cctcacgagc g
//
