LOCUS       c00140_gnlPro..         8125 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BECNEDJF_140.
ACCESSION   c00140_gnlPro..
VERSION     c00140_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:39:19
            Original ID  :: gnl|Prokka|BECNEDJF_140
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8125
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8125
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3335:6315]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3336..6315
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8125
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8125
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             180..2909
                     /locus_tag="ctg140_1"
                     /transl_table=11
                     /translation="MNKRFSTLLAAALVAGSFSSAFAQLEGRNVEWKDAQASRAYYLAG
                     VHFVSPTDYLQAFSLQGLVNGADITAANPVAAAQWTVETKKVGTVTHYAFKNIEGEQYL
                     AFEKKDGAYALAAKKEANDETKSFRWFTVAANGNLQAVLSDEKEPYFLGLKNDGTADWS
                     LALLQSSEHHTDGSTSVIKKKVALYEIYNDQITAEAFASKFGENFTIEFPETLDDMDVF
                     ENVSVEKIANDNLGFKLVDNKGTKDTKDDEYVVLANERVSNSELAANAIGYRYAKMTAK
                     AIKDKKIDDKNTVFYAEENETNIGDGKYALVQKVKVGDLDNKDVTFGKVTNDETTRQLA
                     SVEYDCHSTFMGVTVGKGTRVANTEVANERMLIVNVTAKVADKKNSSALGLKNDFTGAR
                     WYDKVSEADFDQPQGQWLVAAGSAANTIKLINVQKPGFVQDNIRLYEAEGENTYTVFAS
                     DLGLSGLETITLTKVEDYDKLNGYYSTLSDINQKFAFVTTGEYANEEAGSDLYLKADVE
                     GYVVTVVPHAGSEWELIRNEKAQAVTANYKYYDGEKWVELNGKGNEEKGILPADTVRLA
                     YSYAINADELGLGTAWKMAKDDIEFFLKENGKGTYAIVYGATADEVKEYGVINGSEELV
                     AETTDFTNSTPFEIIDLDAAPSLAADSKHMTFEATNSIGFIASDENNNAVLAKEAAALW
                     VDSVNAEGYATPKFFISYAGKMMTTAKKFKEIVDKAYANDEISKKEYAELTADENLYYK
                     SVERIKFVDAERLSGEDTLVIADEKYTGKDIEDFKFKVTENEDGDYVLKANGNYVYVIN
                     GNLVYGTDAKEAEAFVIEVTSAPTANEGIATSEVKVIAGEGNVTIAGAAGKKVVISNIL
                     GQVVANTVVSSDNATIAAPAGVVVVAVEGEAAVKAIVK"
     CDS             3336..5576
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_2"
                     /sec_met_domain="Gly_radical (E-value: 9.7e-47, bitscore:
                     146.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDRDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             5593..6315
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg140_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(6318..6623)
                     /locus_tag="ctg140_4"
                     /transl_table=11
                     /translation="MRRASLYNTFSTSLLLVCSAWLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPVTIREQTLD"
     CDS             6900..7466
                     /locus_tag="ctg140_5"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     NEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             7580..8125
                     /locus_tag="ctg140_6"
                     /transl_table=11
                     /translation="MLDSAMKDQLSGIFSGLVSQYVFDIQVAPDHESRQELIDLLGDVA
                     SCSDHLSCSVADGEGLQFTIVKDGMPTGIRFRAVPNGHEFTSLLLAVLNSDGKGKNIPD
                     ESICARVRALNGPIKLTTYVSLTCTNCPDVVQALNVMATLNSRITHETVDGAINQKEVE
                     AMKVQGVPSVFADGKLINV"
ORIGIN
        1 cttaacaagt acgaaaaacc agctttccaa cacaaaccct ccagaaagcc cctcaaaaat
       61 gactaaaaaa aacgtacgtt ttctctcgtc aaaatacagg aatttggtaa aaacctgtta
      121 aacaggaagt aagaacgata ttcaatatac atattattat taattaaaca ttttagatta
      181 tgaacaagag attttctact cttttggctg ccgctttggt agccggttct tttagttcag
      241 ctttcgcaca gttggagggg cgtaatgttg aatggaagga tgcacaagca agccgcgctt
      301 attatttggc aggggtgcat tttgtgagtc cgacagatta cttgcaggct ttttctttgc
      361 aaggattagt gaatggtgct gatattacgg ctgcaaatcc tgttgctgct gcacaatgga
      421 ctgttgaaac taaaaaggtt ggaactgtta cacattatgc tttcaaaaat attgaaggag
      481 aacagtatct ggcttttgaa aagaaagatg gagcttatgc tttagctgcc aaaaaagagg
      541 caaacgatga aacaaagtcg tttcgttggt ttactgttgc agcaaacggc aatttgcaag
      601 ctgtgctttc tgatgaaaaa gaaccgtatt ttttaggact gaagaacgat ggcacagcag
      661 attggtcatt ggctttatta caaagttctg agcatcatac ggatggatct acttccgtta
      721 tcaagaagaa ggtagctctc tacgaaatct acaacgacca aattacagca gaagcttttg
      781 cgagcaagtt tggcgaaaac ttcacaatcg agttcccgga aacattggat gacatggatg
      841 tgtttgaaaa tgtaagcgta gaaaaaatcg ccaatgataa tttgggtttc aagttggttg
      901 acaacaaagg cacaaaagat acaaaagacg atgaatatgt agttttagca aacgaacgtg
      961 tttccaactc agagttggct gccaacgcaa tcggttatcg ctatgcaaaa atgactgcta
     1021 aagcgattaa agacaagaaa attgacgaca agaacactgt attctatgct gaagagaacg
     1081 aaacaaacat cggagatggt aaatatgcat tagttcagaa agtaaaagtt ggtgacctgg
     1141 acaacaaaga tgtgactttc ggtaaagtga caaatgatga aacaactcgt cagttggctt
     1201 ctgtcgaata tgactgccat tctactttca tgggggttac agtaggcaag ggtacacgtg
     1261 tcgctaatac tgaagttgcc aacgaaagaa tgttgatcgt aaatgtaact gccaaagttg
     1321 cagacaagaa gaattcttct gcactggggt tgaagaatga tttcactggc gcacgttggt
     1381 atgataaagt ttctgaagct gacttcgatc agccacaggg gcagtggttg gttgctgcgg
     1441 gttcagctgc taacacaatt aagttgatca atgttcagaa accgggtttt gtacaagaca
     1501 atatccgtct atatgaagca gaaggtgaaa atacttacac agtatttgct tctgatttag
     1561 gtttgtctgg tcttgaaaca atcactttga cgaaagttga agattatgac aagctgaacg
     1621 gttactacag tacattgtct gacatcaacc agaagttcgc ctttgtaaca acaggtgaat
     1681 atgctaacga agaagccggt tcagatctgt atttgaaagc tgatgtagaa ggatatgttg
     1741 taactgttgt tcctcatgct ggttctgaat gggaattgat ccgtaacgaa aaagcacagg
     1801 ctgttactgc caactacaaa tattatgacg gtgaaaaatg ggttgaactg aatggcaaag
     1861 gtaatgaaga aaaaggtatc ctgccggctg atacagttcg tctggcttat tcatatgcaa
     1921 tcaatgctga tgaattaggt ttgggaacag cttggaagat ggctaaggat gacatcgagt
     1981 tcttcttgaa agaaaatggt aaaggtacat acgctatcgt atatggcgct actgctgatg
     2041 aagtaaaaga atacggtgta atcaatggtt ctgaggaatt agtagcagaa acaactgact
     2101 ttactaactc tactccgttc gaaatcatag acttggatgc tgctccttct ttggctgctg
     2161 acagcaagca catgacattc gaagctacta atagcattgg cttcatcgct tctgacgaaa
     2221 ataacaacgc agtattggct aaagaagcag ctgctttgtg ggttgactct gtaaatgctg
     2281 aaggttatgc aactccgaag ttcttcattt cttacgctgg caagatgatg acaactgcta
     2341 agaagtttaa agaaattgtt gataaggctt atgctaatga tgaaatcagc aagaaagaat
     2401 atgcagaact tactgctgat gaaaatcttt actacaagag tgtagaacgt atcaaatttg
     2461 tagacgctga acgtctgtct ggtgaagata ctttggttat cgccgatgaa aagtatactg
     2521 gcaaggatat cgaagacttc aagttcaaag ttactgaaaa cgaagacggt gactatgtac
     2581 tgaaagctaa tggtaactat gtatatgtaa tcaacggtaa cttggtttac ggtacagatg
     2641 ctaaggaagc tgaagcattt gtaatcgaag taacttctgc tccgacagcc aacgaaggca
     2701 tcgcaacatc tgaagtgaaa gtgatcgctg gcgaaggtaa cgtaacaatc gcaggcgctg
     2761 ccggtaagaa agttgttatc agcaacatct taggtcaggt agtagctaac actgttgttt
     2821 cttctgacaa cgcaactatc gctgctccgg caggtgtagt agttgtagct gttgaaggcg
     2881 aagctgctgt aaaagctatc gttaagtaac gattgatact aaaaaacaat actgtctatc
     2941 gtaagggtgg ttagcgaacc acccttacac aggcacaatg acattttata atcaaataat
     3001 tctagacaac ctgcgtgaaa ccgtatgtaa gaggttttct tgatctacgg tttcaattac
     3061 cctgcgacag gcgaacgagc ggttggattt aatattaata atactaaagt aatgaaatta
     3121 aaagttctac tgcttttccc tctgtgaaga ccggacggca gacaaaaaac aggccccggc
     3181 ggtattcgta ctgccggggc ttcgttttta tttcccttat ccctatttgt aggtattttc
     3241 tccggtttaa catccctcgt cctttacaat ccttttcaga tagggggaaa agacgtatct
     3301 ttgcgtgcct aaaccgaaat agacaagata agcttatgaa gtttgaaaac gagaactggg
     3361 cgcagttcaa aggagaaacc tggaaaaagg agatcaatgt aagagacttt atccagaaca
     3421 attacaaacc ttataacgga gacgatacgt tcctcgtacc ctcttcggag aaaacgaaga
     3481 aagtgtggaa caaactaacc gagatgttta aggttgaaag ggagaaaggg gtatatgatg
     3541 ccgaaacgaa gcttccccag gggattgaca catacggacc cggttacatc gataaggaaa
     3601 acgaagtaat cgtcggcttg cagacagacg ccccgttgaa gcgcggtatc ttcccgaaag
     3661 gcggtatccg catggtcgaa aacagtctgg aagcctatgg ctatcatctg gacccgatga
     3721 caaaagagat tttcaccaaa tacagaaaga cgcacaacga aggcgttttt tcagcctata
     3781 cagacgagat gattgcggca cgtcgttccg ccatcattac cgggttgcct gatgcctacg
     3841 gacgcggacg tatcatcggt gattaccgtc gtgtacctct gtacggaact cagatcctga
     3901 tcgaagaaaa gaaacgtttc cagaaacgtc tcgatataca ggaactgaca gaagaaatca
     3961 ttcagagccg tgaggagata acggagcaga tcaaagcgct gaaagcattt gagaaaatgt
     4021 gtgccggtta tggtttcgat gtgacacgtc cggcaaagga tgcccgcgaa gccgtgcagt
     4081 tcttgtatct ggcttacctg gcagccgtaa aggatcagga tggagcagcc atgtctctcg
     4141 gacgtacttc tactttcctc gatatctata tcgaaaaaga tatttgcgaa ggcaagctga
     4201 cggaagaaga ggcacaggaa ttgatcgacc agtttattat caaactgcgt attgtccgtt
     4261 tcctgcgtac tccggaatac aacgacctgt tctccggcga ccctgtttgg gtgactgaat
     4321 cgctgggcgg acaaggtgtt gacggccgtt cgctggtgac aaagacatct taccgttatc
     4381 tgcatacctt gtataacctc ggcccggcac ccgaacccaa cctgacggtg ctttggttca
     4441 acaatgctcc ggataactgg aaaaacttct gcgcaaaagt atcgatcgat acctctgcca
     4501 tccagtacga gaacgacgat ctgatgcgtc cggactatgg cgatgattac ggtatcgcct
     4561 gctgtgtgtc tccgatgaag atcggtaagc agatgcagta tttcggagct cgtgccaacc
     4621 tggcaaagtg cttgttgtat gcgatcaatg gcggacgtga cgagaaatcg ggtgtgcagg
     4681 tggctccccg tttcgaaccg atcaccagcg agtatctgga ttacaacgaa gtgatggaga
     4741 aatacgagca gatgatgcgc tggttggcaa aagtctatgt caatgcactg aagatcattc
     4801 actacatgca cgacaaatat gcgtatgaag cattcgagat ggggcttcat gacggagatg
     4861 tggaacgtat ccgcgcaacc ggtatcgccg gcctttctat cgttgccgac tcgctggctg
     4921 ccatccgtga tacgaaagtg aaagtgatcc gtgacgaacg tggtttggct gtcgacttcg
     4981 aacgcgaagg cgaatatgta cctttcggaa acaatgacga ccgcacggac agtattgctg
     5041 tcgacattac tgagaagttt atggaatatc tgcgccagca tcagacatac cgcaaggcaa
     5101 ctccgacaca atctatcctg acgatcacat caaacgtggt ctacggaaag aaaaccggaa
     5161 cgactcccga cggccgtccg ggtggtacgc cgttcgctcc gggagctaac ccgatgaacg
     5221 gacgcgatac gaaaggggct gtcgccgctt tggcttctgt agccaaactg ccttttcagc
     5281 atgcgcatga cggtatttcc tatacatttg ccgtttcacc ggctacgttg ggtaaggagc
     5341 gcgatattca gataaataat ttggtgagcc tgttggatgg ctattttacg cctgacggtg
     5401 gccagcatct gaatgtgaac gtgttcgata gggacttgtt gttagatgca atggagcatc
     5461 cggagaaata cccgcagctt acgatccgcg tttctggcta tgccgtcaac ttcgtgaagc
     5521 tgacacgcga acagcagctc gacgtcatct cgcgcaccat caacagcagt ttataattgt
     5581 ccattgatta atatgttggg gcgtatacat tctttagaga gtttcgggac cgttgacggt
     5641 cccggaattc gctttgtcgt ttttatgcag ggatgtccgc ttcgttgtct gtattgccac
     5701 aatccggata cctgggaggt aaagcgtgaa acgccttatc tgctggaacc tgaagcgttg
     5761 cttgccgaag tgctgcgcta taagaatttt atcgctaaag gtggtgttac cgtgaccggt
     5821 ggcgagcctt tgttacaacc tgaattcctt aaagagtttt tccgcctttg ccgtgagaat
     5881 ggtattcata cggcgttgga cacttccggc tatatctgtt ccggtaaagc attggaggtg
     5941 ctcgaacagg tcgatctggt tctgttagat atcaagacga tcgacgccgg tcttcatccc
     6001 cgtttgacgg ccgtaaaact cgataatact ttacgctttc ttgatgaact ggagaaacgc
     6061 ggcatccctg tatggattcg ccacgtgatc gtgcccggcc tgacggacga tgatgaagcc
     6121 ttgagcaagc tggccgaata tatcagttca tataacgtgg tccagaaagc cgaacttctg
     6181 ccttatcata caatgggagc ttacaaatat gaggcacaag gcctggacta taagctgaaa
     6241 ggtgtggaac cgctttcagc agaacgtctg gccaatgcga aagcgatctt tatgaaacac
     6301 ggggtaaccg tataagccta atcgagtgtc tgctctctga tggtgactgg tttacggctg
     6361 atccggttga ctgtgcggac gctgtcgctt tcaatactgg taacatctcc ctcgcagttg
     6421 gtgaaataga tgtaatgtcc catatccatg aaacgataga ccttacaccc ttcgtgttcg
     6481 aacagatatt cgacttgata agtggaattg tttaccggtt tttcctttgt aaggggtatt
     6541 tgtgagacgc atgaagaaag ccatgcgctg caaacgagca atagacttgt tgaaaatgta
     6601 ttatacaaac tagctcttct catcttcgaa tatttttaag ttaacatcca aaggtaacaa
     6661 ttaaagaatc acatggtatg accgaaaggt attttaccta caattaggta tatttttatt
     6721 gtttcggaaa acattacttt gcttcctatt gtcatagtag ataagtatgc ttgcagatag
     6781 aagtccgttt atcggcagat aaaaattatc aatttgaaaa attctttcaa tcagtatctt
     6841 acgatttacc tttgtagggt aaacaaaaaa tagtagtaac aaattaaata gtaaaaatca
     6901 tggaaccaat tatcaattca caagtacccg aattcaaagt acaggctttt cagaatggta
     6961 gtttcaagac agtaagtagc gaagatatca agggcaaatg ggccatcttt ttcttctatc
     7021 ctgccgactt tacgttcgta tgtccgaccg aattggtcga tatggctgac aagtacggca
     7081 aatttcagga aatgggtgtc gaagtatatt ccgtaagtac agactcgcac tttgtacaca
     7141 aagcatggca tgatgcttcc gaaacgatcc gtaagatcaa atatccgatg ctggcagacc
     7201 cgacaggggc tttaagccgt gcgttcggtg tgatgattga agaagagggt atggcttatc
     7261 gcggtacgtt cctggtaaat cccgagggaa agatcaagat tgccgaaatc cacgataacg
     7321 gtatcggccg taatgcagac gaattgcttc gtaaagttga agctgcccag ttcgttgctt
     7381 ctcacccgaa cgaagtttgt cctgcaaagt ggaaaaaagg cgaagcaacc ttgaagccga
     7441 gcatcgactt ggttggtaag atctaaaaag aatgatgtgc caattgatta atgtgccaat
     7501 tatttatgag gcaatacatc gctttagtcg attggtacat tctttaattg gcacgttaat
     7561 aaattgacat attattttta tgttagactc agcaatgaaa gaccagcttt cgggtatttt
     7621 ctccggactg gtatcccaat atgtatttga tatacaagtc gctcccgatc acgaaagccg
     7681 tcaggagcta atagacctgc ttggtgatgt ggcatcatgc tcggatcatt tgtcatgcag
     7741 cgttgcggat ggcgaaggac ttcagtttac aatcgtaaaa gacggtatgc ctaccggtat
     7801 ccggtttcgt gcagtaccta acggacatga gtttacatcc ttgttgttag ctgtcctgaa
     7861 tagtgatgga aaaggtaaaa atataccgga cgaaagtatc tgtgctcgtg tcagagcctt
     7921 gaatggtccg atcaagctga cgacttatgt ttctcttact tgcaccaatt gtccggatgt
     7981 agtgcaggca ctcaatgtca tggctactct caatagccgg atcacccacg aaacggtgga
     8041 tggcgctatc aaccaaaaag aagttgaggc aatgaaagta cagggagtcc cttctgtttt
     8101 tgccgatggc aaactgatca atgtg
//
