LOCUS       c00005_gnlPro..         7371 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IPJHGKPK_5.
ACCESSION   c00005_gnlPro..
VERSION     c00005_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:54:39
            Original ID  :: gnl|Prokka|IPJHGKPK_5
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7371
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7371
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4178:7137]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4179..7137
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7371
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7371
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             41..1039
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     -3.5; E-value: 38)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg5_1"
                     /sec_met_domain="LysR_substrate (E-value: 1.2e-47,
                     bitscore: 150.8, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 5.2e-19, bitscore: 57.0,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVKLKISIFALIDSISLINQRTMTIQQLEYILAVDQFRHFAKA
                     AEYCRVTQPTLSAMIQKLEDELGVKLFDRSMTPVCPTAIGKKVLEQARKILSEVICVKE
                     IISEEQHSLSGTFRLAVLPTIAPYLLPRFFPQLMEKYPDLDIRVMEMKTPDIRKALLTG
                     EADAAIIASMLDDAALTEETLFYEQFLGYVSKKEPLFKHDVIRTSDVTGERLWLLDEGH
                     CFRDQLVRFCQMEAVKISQMAYRLGSMETFMHMVESGKGITFIPELAVMQLSEEQKELV
                     RPFAIPRPTRQIVLVTRKDFIRTSLLQVLKEEIQAAVPKEMLTLQAVQCLV"
     CDS             complement(1144..1896)
                     /locus_tag="ctg5_2"
                     /transl_table=11
                     /translation="MKKFNLITFAVILMLLPILQSCLDDANNDEWVTCPPGGILAIGTM
                     KIPNADTPRDFFIALDNGDNVLPADTADIRNRKYTVAEGQRVFVGYLQMGEEKPGYENG
                     KIFTIEDILTKEIIPLTEATADSIGDDRINVTAHALTKDYLTIEYQYLGSMNENKKHML
                     NLVQNEITGPIKDDGYIYLEFRHNAFNDSPNQLGSSLVSFKLDSIAEQLATAKGIKLRV
                     NTIYDNIQYVTIDINEDKNLKIKSFHSQ"
     CDS             2084..2632
                     /locus_tag="ctg5_3"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTVLQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
     CDS             2687..3946
                     /locus_tag="ctg5_4"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVVTTAVSLYFLNSPVADEIRYATAPSLAVNPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLVEKRPEAVTEEKRSEPQQEAIAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSENFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVVDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(4179..4904)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.9;
                     E-value: 1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg5_5"
                     /sec_met_domain="Radical_SAM (E-value: 3.5e-31, bitscore:
                     97.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEKGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
     CDS             complement(4909..7137)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg5_6"
                     /sec_met_domain="Gly_radical (E-value: 2.5e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTSETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             7255..7371
                     /locus_tag="ctg5_7"
                     /transl_table=11
                     /translation="MGRSQIAVITSKYTFLIFFCGIICKLKNDSYLCIALREK"
ORIGIN
        1 atatttcttc tttttatctg gaacaaagat acggcacaaa atgattcgtg tcaaactgaa
       61 aatttctatc tttgcattga tagattctat atctttaata aatcagagaa cgatgactat
      121 acaacaattg gaatatattc tggctgtgga ccagttccgg cattttgcta aagctgccga
      181 gtattgccgg gtgacgcaac ctactttgag tgcaatgatc cagaagctgg aagacgaatt
      241 gggggtgaaa ctgttcgacc gtagcatgac accggtttgc cctactgcca ttggtaagaa
      301 agtgctggaa caggcccgta agattctttc ggaagttatc tgtgtcaagg agatcatcag
      361 cgaagaacag cactccctgt cgggtacatt ccgattggca gtgttaccga cgattgcacc
      421 ttatcttctg ccccgtttct ttccgcaact gatggagaaa tacccggatc ttgatatacg
      481 ggtgatggag atgaagacgc ccgacattcg caaggctctg ctgaccggtg aagcggatgc
      541 tgctatcatt gccagcatgc tggatgatgc tgcattaacg gaagagacct tattttacga
      601 gcaattcctg ggctatgtct caaagaagga accgttgttc aagcacgatg tgattcgtac
      661 gtccgatgtg acaggggaac ggctttggct tcttgacgaa gggcattgct tccgcgatca
      721 gttggttcgt ttttgtcaga tggaagcggt taagatcagc caaatggcgt atcggctggg
      781 tagtatggaa acctttatgc atatggtgga gagtggtaag gggattacgt ttattcctga
      841 actggcagtg atgcaattga gcgaagagca gaaagaactg gtccgtccgt ttgctatccc
      901 gcggcctacc cgtcagattg tcctggtgac ccggaaagac ttcatccgca ccagtctgct
      961 gcaggttttg aaagaggaga ttcaggcggc ggttcccaag gagatgctta ccctgcaggc
     1021 cgttcagtgt ctggtataat ataaaaacga cggcgatatt ttcacaaaca tcgccgcctt
     1081 atttctttta tgtatcagaa ttattactcg ttttgcctga ttgtgtaggt gtttcttgtt
     1141 gttttattga gaatgaaatg attttatttt taagtttttg tcttcgttga tatctattgt
     1201 gacatactgt atattgtcat aaatggtgtt cacacggagt tttattcctt tggctgtcgc
     1261 caattgctcg gctatactat ccagcttgaa agaaacaagg ctcgaaccca attgattggg
     1321 ggagtcatta aaggcattat gccggaactc cagatagata tatccgtcat cttttatggg
     1381 acctgttatt tcgttttgta ccaagttcag catgtgtttt ttattttcat tcatgctgcc
     1441 cagatattga tattcgatgg tcagataatc tttcgtaagt gcatgcgctg tgacgttgat
     1501 acggtcgtct ccaatgctgt ctgctgtagc ttccgtcagt gggatgatct ctttcgtcaa
     1561 tatatcttct atcgtgaata ttttaccgtt ctcatatcca ggtttctctt ctcccatctg
     1621 cagatagcca acgaagactc tttgaccttc tgcaacagtg tatttcctgt tgcgtatatc
     1681 ggccgtatct gccggaagta cattgtctcc attgtccaat gcaatgaaaa agtcccttgg
     1741 agtgtccgca ttgggaatct tcattgtgcc gatggccaat attccacccg gaggacaagt
     1801 gacccactcg tcgttgttgg catcgtccag acatgactgt aaaatgggca ataacatcag
     1861 gatgacggca aaagttatta aattaaattt cttcataagt ttcttgttgg ttttatccta
     1921 tttatccatt gagatttatt ctttattgta ttgagaaaat gcaggaagtg atgaaatatt
     1981 gcatcctaaa atgttaaaga aacaaaagtg ataaaagagt tgttctctct gtgcagtatt
     2041 tctatctttg ctgcatttat aaggtaaaca cgtgtttagg agaatgaatg aattagagct
     2101 gtcggaacgt tgcaggcagg gagataatcg tgcccgcaaa gaactttatg agcagtacgc
     2161 agggcgcatg cttggtgtct gtcttcgtta tgccggagac agggacatgg cacaagactt
     2221 ggtacacgac ggtttcctga agattttcga ctccttcgac aagtttacct ggcggggtga
     2281 aggctctctg agagcatgga tggagcgtgt aatggtgaat acggttttac agttcttaag
     2341 aaagaacgac gtgatgaacc agaccacggc actggatgaa gttcccgaaa cgtatgaaga
     2401 accggatgct tcggctgttg aagcaatacc acagaaagtg ctgatgcaat ttattaatga
     2461 actccccgcc ggatatcgta ccgtattcaa tttatacacc tttgaagata agtcgcacaa
     2521 ggaaatcgca cagatgttgg ggattaatga aaaatcttct gcctcacagc tttttcgcgc
     2581 aaaaagtgta ttggcaaaga aagtgaaaga atggttggtg accaatggtt gacatgtatt
     2641 tacagatact gaggtgaacc ttggatttca aaaatgaaat agaatgatga aagaagacga
     2701 aaaatggatt aaagcattca aggataagct cgaagattat tcggagccga tgcctgcttc
     2761 cggttgggaa cgactggaaa gggaactgat gcctgtaacc gaaaaacgaa tatatccgta
     2821 tcgccggtgg gcggttgctg cggcagctgt ggtattggtg gtaacgactg ctgtcagttt
     2881 gtattttctg aatagtccgg tagctgatga aatccgttat gcaacggctc cttcactggc
     2941 tgtcaacccg gatgtattgc cggaacctgc cttgccggat gtacaggtag ctgtatccga
     3001 accagttaag ccggtaggaa cgacgagtat taaccctgtt tcgggctatt tggcaaagaa
     3061 tactgatccg gtgattgttc cggaggtatc ctccctggta gagaagcgac cggaggcagt
     3121 gacggaggaa aagagatctg agccgcaaca ggaagcaatt gctgcaatcg agaaaaaaga
     3181 aagtgcgacg gcgcagcctc caaagaggaa agaagcgcgg cgaccgtccg gtaaagacaa
     3241 gtatcagttg cctatcggag actcttctgc taaacgtggt ggtaaatggt cgatgggagt
     3301 gggtatcggt aatggaggtg gacttccgac aaatggttcc gaaaattttg cgccccgtcc
     3361 gatgactaac agggtcgact tgatgacgat catgaatgga gctgtgagta ttcctgccga
     3421 tcaggaagtt atatttgaag aaggagtccc ttacctgaag tccaatacaa ctgccgtggt
     3481 ggattatgaa catcatcagc cggttagctt cggactgtct gtacgtaagt ctcttcccaa
     3541 aggtttctct gtagagacgg gattgactta tacgttgttg tcttctgaca tcaaacggca
     3601 gggggatacc aagatgcaga gtcagaaatt gcattatata ggtattcctg tacgtggaaa
     3661 ctggaatttt ctggaaaaga aatattttac actctatgta tctgccggag gaatggttga
     3721 aaagtgtgtt tatggaaagt tggcggatga taaggtgaat gtgaagcctt tgcaattttc
     3781 tgtagccggt gccgtgggag cgcaattcaa tgcgacagat catgtcggcc tatatgtgga
     3841 accgggagtt tcgtatttct ttgatgatgg ctccaaagta caaaccatcc gaaaagaaag
     3901 gccttgtaac tttaacttgc aggcaggatt gcgattcact tattaaagta ggatggcaaa
     3961 aaatatgccg gttacattat ctccctgttc ttgccttgta ttgtaatgaa ttgaggcaag
     4021 aacagggaga tttttatgtc gtgacttaca aaattataaa taagcgcaat cgtcataatt
     4081 caccgcagag tgaacttaat ctgttgattg ccaatgaatc tttatctgtg taaattcgtg
     4141 taatctgtgg cgaatacgaa tttcgacact tatataagtc aattcacttg tacggtccgg
     4201 aagtattgcc tgaatagttt ctcagccttt tctatctgct ccggagtatt ctctttcact
     4261 cctttcagca tatattcttt gttcattgct tcatatttgt gaacacccaa ggtgtggtaa
     4321 ggcaatattt ctacacgctg aatcatctgg tacgttccaa gatgctctcc cagttgccgg
     4381 atatcgtctt cgaagtcact gtagccgggc actaatacat atcgcaacca gaatggttta
     4441 tgattggttt caagccaggc tgcggttttc agggtctgct cgttgcttct tccggttaaa
     4501 gactgatgat gttccggatt aaattctttg atatctaata aaaccagatc agtcaattcc
     4561 aatagttcct ctacatcatt gttccatagc ccgccattgg tatcaaggca aatatggatt
     4621 cctttttcct ttagtctttt aaaaagtggg ataagcgatt tagcttgaaa agtaggctct
     4681 ccgccggaaa aggtgatacc tcccttttta ccgaagaaag ctttctgact gacggccatg
     4741 cgaactattt cctcagggtc ggttgctgtc cctcctttgg cttcaattgt gtccggattg
     4801 gcacaataga ggcaacggaa gttacaccct tgaaggaaaa cgacgagccg gagacccggc
     4861 ccgtcgaatg ttcccattga ctcgtatgag tgtacgttta tcattcaatt acattctttc
     4921 gtggaaactg cggctgatta cttccaactg atgttcgcgg ctcaatttca cgaaattaac
     4981 ggcatatcct gagacacgga tagtcagttg cggatacttt tcgggatgtt ccatcgcatc
     5041 ctgcaacatt tcccggttca gcacgtttac gttcaggtgg tgtgcgcctt ttgtaaagta
     5101 tccgtccatc atggtcacca gattgtcgat acggtcttct tgtgttgctc ccaatgattt
     5161 gggaacgatt gagaatgtat tgctgatacc atcctgtgag tcgcgataac gcaacttggc
     5221 tacagaactc agtgaggcga ttgcaccatt tttatcacgt ccgtgcatgg ggttggcacc
     5281 gggagcaaag gcaatgcctt tggcgcgtcc gtcaggagtg gcaccggtct tcttgccata
     5341 cattacgttc gaagtgatag tcagcagcga gagggtagga cgggcattct tatatacggg
     5401 gagtttcttt aattcttcgc taaagaaata taccaggtcc acacccaggt ggtctacacg
     5461 gtcatcgtca ttaccgaaac aagggaaagc accttcgata tcgaatccct ccgtcagtcc
     5521 gatgtcgttg cgacgggctt tcacatgtgc atatttgatg gcggacagtg aatcgatggc
     5581 gattgaaagt ccggctacac cataagcgag gttaatgcgg ggatcagtat ccacgaaagc
     5641 catctgcgcc ttttcgtagt aatacttatc gtgcatgtaa tgtatgatat tcattgcttc
     5701 gttgtataca cgtgcaattt gtatcaatac tttcttatag ttggccatta cttcttcgaa
     5761 cttcagtgtt tcactggtca atacagggat gtcttttacc ataactgtac ctgtattttc
     5821 gcaacgtcct ccgttgatag ccagtagcag ggctttggca aggttggcac gtgcgccgaa
     5881 gaactgaatc tgctttccga tttcctgata tgaaacacaa caagcgattc cgtagtcatc
     5941 cgattgacgg acttcacgca tcaggtcgtc gttttcgtat tgtacggaag aagtgtcgat
     6001 agatacttgt gcacagaatg ctttgaaacc ttcgggtaac tcagggctcc atagtacagt
     6061 catgttaggt tccggtgacg ggcctaagtt ataaagtgtc tgcaagaagc ggaaagaagt
     6121 tttagtaact ttggtacgtc cgtcattgaa acgtccaccg atagattcgg tcacccatgt
     6181 gggatcaccc gcaaaaatat cgttgtacga ctgcatacgc aagtggcgta ccatacggag
     6241 tttgataacg aattggtcga tcagttcctg agcgaatgtt tcggttatcg taccctggct
     6301 taattcatat tcaagataga tgtcgaggaa tgaagataca ttacccagtg acatggcagc
     6361 accatcctgc tcttttacgg cagccagata agccatatac acccattgta cagcttcctg
     6421 tgcggtatat gccggtcggc tcagatccaa accataatat tcgcccaata ctttaatctc
     6481 tttcagggcc ttgatttgct cggctacttc ttcgcgaaga cgaatgcggg cttcagtcat
     6541 aggacctgtc agattatgca gatcttcttt tttagcttct atcagacggt caagaccata
     6601 gagagccagg cggcgataat caccgatgat acgtccgcgt gcatagttgt cgggaagtcc
     6661 ggtaaggaat cccaatgaac ggaacgaacg aatttcttcg gtatacacat cgaatacccc
     6721 atcattatga gtcttgcgat agtgagtgaa gatatcctta actctgtcat ctacttccac
     6781 tccgttttca cggcaagctt tcataaccac attgattccg ccaaacggtt tgatggcacg
     6841 ttttaacacc tgatcggtct gtagtccgac gatcagttcg ttctctttat cgatgtagcc
     6901 ggcagggtga gaggttatag tagatacagt tgtgtagtcg agtgcacgaa taccattatt
     6961 gtttctctct tcttcgagag ctttgagaca gtgattccag acagctttgg tgcgttcggt
     7021 aggtccttgc aggaaagaag catccccttc ataaggagtg atgttgttac taacgaaatc
     7081 ccttacattg atttctttgc tccaaagtcc atctttaaag gtcttattca gttccataaa
     7141 attaatcagt tagtggttag ttatccttta gagcgcgcaa atttacaacc ttttttcggt
     7201 ttaggtatat aaaagggtcc tattttatgt tgaatagcat aaaaatacct atttatgggg
     7261 agaagccaga tagcagttat tacttccaaa tatacttttc taatattttt ttgcggaata
     7321 atttgcaagt taaaaaatga ttcgtacctt tgcatcgctt taagagaaaa g
//
