LOCUS       c00576_gnlPro..         3440 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GICKJOJA_576.
ACCESSION   c00576_gnlPro..
VERSION     c00576_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 12:55:43
            Original ID  :: gnl|Prokka|GICKJOJA_576
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3440
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..177
                     /locus_tag="ctg576_1"
                     /transl_table=11
                     /translation="DLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKLG
                     VGSTFWAWLPLRP"
     protocluster    1..3440
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[347:3323]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      348..3323
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3440
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3440
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             348..2588
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg576_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.2e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2589..3323
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg576_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 gatttgcatg acaaagtatt tacccgtttt gagaaattga atgagttctc gcagggtacc
       61 ggacttggct tgacgatctc cagagctatc gtagaagcgg cagggggaga agtgggcttc
      121 acttcaaaac ttggtgtcgg atctactttt tgggcatggt tgcctcttcg cccttagggc
      181 tgactcccca tttattggta atgtcctttt aaaatagtga aggatgagaa gcttttttat
      241 cgcaatttat agggatttta gaactggtgt agcatatgtg acagaaatat gacgataatt
      301 ctcctacctt tgcggcggta aaaatgtggt attagataag attgtttatg aaatttgaga
      361 atgagtgctg ggatcacttt aagggtgaga cctggaaaag agagatcaat gtaagagatt
      421 ttatccagag taactatacg ccttatgaag gggatgattc ctttttagtc gcttcttccg
      481 agaagacccg taaggtctgg aacaagctga ccgagatgtt taaggtcgag agggaaaagg
      541 gagtttatga cgccgagaca aaattacctc aagggatcga tgtatatggc ccgggctata
      601 ttgataagga gaatgaggtg atcgttgggt tacaaaccga tgctccgctg aaacgtggta
      661 tcttcccgaa gggcggtatc cgtatggtcg agaatagctt ggaagcttat ggttatcatt
      721 tggacccgat gacgaaagag atctttacga agtatcgtaa gactcataac gagggcgttt
      781 tctccgcata tacggaagag atgattgccg cacgtcgttc ggctattatc accggtctgc
      841 ccgatgcgta tggcagagga cgtattatcg gtgactatcg tcgtgtcgct ctgtatggta
      901 cggcaatctt gatcgaggag aaaaagcgtt tcttgaatcg tttggatatc caagagatca
      961 cggaagagat catccagagc cgtgaggaga tttccgaaca gatcaaggct ttgaaagcgt
     1021 tcgagaaaat gtgcgctagc tatggctttg atgtgacccg cccggcacaa aacgctcgtg
     1081 aggcggttca gtttgtgtac ttggcttatc tggcggcggt aaaagaccaa gatggcgcgg
     1141 cgatgtctat cggacgtact tctacgtttt tggatattta tatcgagaaa gacatccgtg
     1201 agggtaagtt gaccgaagag gaggcgcaag aattggtaga ccagctgatc atcaagttgc
     1261 gtatcgttcg tttcttacgt acgccggaat acaatgattt gttctccggt gatccggttt
     1321 gggtaacaga gtccttgggc ggccaaggag tggatggccg ttctttggta acaagaactt
     1381 cctatcgtta tctccataca ttatataatc taggtccggc accagaaccc aatttgacgg
     1441 ttctgtggtt taagaacgct cccgagaact ggaaacgttt ctgtgccaag gtctctatcg
     1501 atacgtcggc tatccaatat gagaacgacg atttgatgcg tccggattat ggagatgatt
     1561 atggtatcgc ttgttgcgtt tctcctatga agatcggtaa gcagatgcag ttcttcggag
     1621 cccgtgcgaa tttggcgaaa tgtttattat atgcgatcaa cggaggacgg gatgagcgca
     1681 gcggcgtaca ggtagccccg atgttcgagc cggttcgcgg cgagtatctg gaatacgatg
     1741 aggtgatggc taagtatgag caaatgatgc gctggttggc gaaagtgtat gtgaacgcct
     1801 tgaagataat ccattatatg catgataaat acgcgtatga ggcattcgag atgtcgcttc
     1861 acgatgggga tgtagagcgt atccgtgcga caggtatcgc cggtctttct atcgttgccg
     1921 attcgttggc tgctatccgt gatacgaaag tacgtgtgat ccgtgacgag cgtggcttgg
     1981 cggttgattt cgaacgtgag ggcgaatatg ttcctttcgg taataacgat gatcgtacgg
     2041 atagtatcgc tgtagagatc accgagaagt tcatggaata tttgcgtcag cacgagacct
     2101 atcgccatgc gaaacctacg cagtccatcc ttacgatcac ctctaatgtg gtatatggta
     2161 agaagactgg tacgacaccg gatggccgta tcggtggaac tcctttcgct ccgggcgcga
     2221 accctatgaa cggtcgtgat acgaaaggtg ctatcgctgc gctagcttcg gtagcgaagc
     2281 ttccattcca gcatgcgcac gacggtatct cttatacttt cgctgtttct ccggctacgt
     2341 tgggaaagga gcgtgaagtg cagatcaata atctggttag cttattggat ggctatttta
     2401 ctccggatgg aggtcagcat ctgaatgtta acgtatttga taaggatttg ttgatcgacg
     2461 ctatggaaca tcctgagaaa tatccgcaac ttacgatccg tgtatccgga tatgcggtaa
     2521 acttcgtgaa actgactcgt gaacaacagt tggatgttat ttcaagaacc attaaccatt
     2581 cactgtaagt gataaaaggt aagatacatt cattagagag tttcgggacc gtagatggtc
     2641 ccggaattcg ctttgtcgtt tttatgcagg gctgcccgtt gcgttgcctg tattgccata
     2701 acccggatac ttggaacccg aaagggaagg tgaaatatca gatgactccc ggcgagctat
     2761 tgacggaagt cttacggtat aagagtttta tcgcccgtgg gggggtgact gttaccggtg
     2821 gggaaccttt attgcaacct gagtttctga aagagttttt ccgtttgtgc caagagcaag
     2881 gtcttcacac agctttggac acatctggtt tcgtatgtac ctcaaaagct tgggaagtct
     2941 tggattatgc ggacttagtg ttattggaca tcaaaacctt aaacccggat ttacatccgt
     3001 tactggcggg tgtcaagcag gataatacgc ttctgttctt ggatgaattg gagagacgtg
     3061 gcatagatac ttggattcgc catgttattg tccccggata tacggataat gacgagtggc
     3121 tggaggcgtt agctcggtat gtcagttcat ataaagtggt tcgcaaagtg gaattattgc
     3181 cttatcatac gatgggaacc tataaatatg agcaattagg cctcgattat ccgttaaagg
     3241 gggtggaacc cttatcaaaa gaacgtttag ataatgcgaa agcgatattc tctcgctaca
     3301 aaccagaaaa taacaaggcc tgatctctcg atcaagcctt gcgattatgc aacagctaat
     3361 cagggaaaac aatctaatcc gattagcatt taaacgatag taataatttt ttgcctagac
     3421 taaccttgac tatttaatat
//
