LOCUS       c00421_gnlPro..         5365 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KELFKKGH_421.
ACCESSION   c00421_gnlPro..
VERSION     c00421_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:19:50
            Original ID  :: gnl|Prokka|KELFKKGH_421
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5365
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5365
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[509:4721](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      510..4721
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..5365
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5365
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             510..4721
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg421_1"
                     /sec_met_domain="HGD-D (E-value: 8.1e-24, bitscore: 72.0,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 6.1e-35, bitscore:
                     108.0, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVYKVGLDIGSTTTKMVVLDKDGTTVLFSGYKRHQAKIQECLLG
                     FLYQIKEQLGDIPLSFHITGSVGMGISEKCSLPFVQEVVAATNYVCREHPHTATMIDIG
                     GEDAKVVFFQDGQATDLRMNGNCAGGTGAFIDQMAILLGVSIDELNELALRSNQVYSIA
                     SRCGVFCKTDIQNLIAKNISKENIAASIFHAVAVQTTVTLARGCNIVAPVLLCGGPLTF
                     IPALRKAFANYLNLSQDTDFILPEKGNLIPAWGAALAENNEESIHLSNLIQVIENKLSV
                     TSPFQSDLSPIFDSEEEYRSWKKEKDQYKIQYASLRPGLQEATIGIDSGSTTTKIVVLD
                     NNHRILYSYYHDNNGNPIKTVENGLQKLYEECRRRGTTLRIKGGCSTGYGEDLIKAAFH
                     MDAGIIETIAHYAAAKHISKEVSFILDIGGQDMKAIFVNDGVINRIEINEACSSGCGSF
                     ISTFAQSLDYSVEDFAKAACFSQAPCDLGTRCTVFMNSKVKQVLREGASVADIAAGLSY
                     SVVKNCLYKVLQLKDTEILGDHIVVQGGTMRNDSIVRSLEKLTGKQTFRSNCPELMGAL
                     GCALYAKQIENARVTELNEMLQWAQYTSKQLQCRGCENQCAIMRYTFNNDNHYFSGNRC
                     EKVFSNKGSHADKGINTYDKKLELLFDRSADIPQPLFTIGIPRILNMYEEYPFWHTLFT
                     ACGIQVQLSAPSTFSKYETAAGMVMSDNICFPAKLVHSHIRNLTLQNVNRIFMPFVVFE
                     KKDKQQQNSYNCPIVSGYSEVIKSVQEENIPIDAPTITFKDEALLYKQCYEYLKSLGIR
                     DEVCKNAFSRALQEQYAFEEKIAAYNQEVLNEGREKHKLIILLAGRPYHSDPLIQHKVS
                     DMIAAMGVYVITDDIVRQQEISLEKTHYLSQWAFTNRILKATKWAAMQEGDIQYMQMTS
                     FGCGPDAFLIDEVRNLLKRYGKNLTLLKIDDVNNIGSIKLRVRSLVESLNFSLKHSQAK
                     DPEPFVSTAPFTKKDKKKKILAPFFTPFISPLIPSIMKVAGYEMETLPLSDTASCDWGL
                     KYSNNEVCYPATLIVGDIVKAFKSGRYDPANTCVAITQTGGQCRASNYISLIKKALIEN
                     GYTNTPVVSLAFGSGIENEQSGFKVNWLKVLPIILASVLYSDCIAKFYYAAVVREKERG
                     QAARLRDLYLDTAQPIIQKNKPEDLLSYLYLAARDFNKICEQRSCHKVGIVGEIFLKFN
                     PFAQKDVTSWLINQKIEVIPPLISDFFMQGFVNLKVRQNQHLQRKLTPDFVIDWLYKKV
                     QKQINKVNEIGKNFNYFTPFESIFEKAEKAKQVISPGTQFGEGWLISGEILSFASQGVN
                     HVISLQPFGCIANHIVEKGIEKRIKSLCPQMNILSLDFDSSVSDVNITNRLLLFIDNIN
                     N"
     CDS             4726..5346
                     /locus_tag="ctg421_2"
                     /transl_table=11
                     /translation="MATTEHSDLEQQIIKTAQQLFIEKGFVETSMSDIAATVGINRPTL
                     HYYFRTKDKMFKAVFGSIVMSLMPKIQDIVKQDIPFMERLSMILDEYIELFTSNPCLPR
                     FICGEIQRDVNHLLATAKELQFGDALSQVKDSILTAMEDGKLKKVPIHIVFLTVYSLLS
                     YPFLAKNLIVSLFLEDEAAFADFLQEWKQNIINQLQNLLCYEE"
ORIGIN
        1 tgccaaatgc atgaggaaaa agcagcatag ccaaaagaaa atatagcaga actgtaaaat
       61 ataaaagagg ttgtgccaac gttttgacac aacctcattt tgtttataat tcagcttaaa
      121 atctctcgtc tgatcctaga actgttctac aatagtttat aataaactcg aataccgttt
      181 tactaaaact atcaaaaagt ttattataaa cagaatccgg gtttattaaa agccacgtta
      241 ccttttgtca aaaatagagt aaacaagatt tctaagattc accataaaag atctactaca
      301 acccaacaag taatataaat acacaattat tcatttacgt ctttttttac acgatcgttt
      361 tcctccctca cattccatct ttcttttttc ccaattcgtt aattaaatca aaaacttcaa
      421 ttacgatttg ccgtatctac catattttta catacatttg cgtctgacac agatgtcaaa
      481 cacgaggtct aatttaagat atttggtata tgaatgtata taaagtgggt ttagatatag
      541 gctctactac cactaagatg gtagtattgg ataaagatgg aactacagtc ttgttttccg
      601 gatacaaaag gcatcaggca aaaatacaag aatgtttact cggatttctt tatcaaataa
      661 aggaacagtt gggtgatatt cctttatctt tccacattac gggctctgtt ggaatgggaa
      721 tttcagagaa atgctctctg ccgtttgtgc aagaggttgt agctgcaaca aactatgtgt
      781 gccgggagca tccgcacaca gccactatga tcgatattgg aggagaagat gcgaaagttg
      841 ttttctttca ggacggacag gctacagacc tgcgcatgaa tggaaattgc gccggcggaa
      901 ccggggcttt cattgaccag atggctattc tgcttggagt ctccatcgac gaattgaatg
      961 aactagcact tcgttccaat caggtatact ctatcgcttc acgctgcgga gtattctgca
     1021 aaacggacat acagaatctg atagccaaaa atatatctaa agaaaatata gcagcttcca
     1081 ttttccatgc agtagccgta cagaccactg tcacacttgc acgcggttgc aatatcgtag
     1141 ctccggtatt actctgcggt ggcccattga cttttattcc ggcccttcga aaagcatttg
     1201 ccaactatct gaatctgtca caagatacag atttcatatt accggaaaaa ggaaatctga
     1261 tccctgcctg gggagcagca ttggcagaga acaatgaaga aagtattcat ctctccaacc
     1321 tgatacaggt aatagaaaac aaactaagtg ttacctcacc tttccagagt gatctttctc
     1381 ctatattcga cagtgaggaa gaatatcggt catggaaaaa agaaaaagac cagtataaaa
     1441 ttcaatatgc cagtctgcgt cccggattac aggaagcaac tatcggcata gactccggct
     1501 ctactaccac caaaatcgta gtactcgaca acaatcatcg tatcctttac tcctattatc
     1561 acgacaacaa cggaaaccct ataaaaacag tagagaacgg cttgcagaaa ctatatgaag
     1621 aatgccggag acgaggaact accctgcgca taaaaggagg atgttctacc ggttacggag
     1681 aagatctgat aaaagcggct ttccacatgg atgcgggaat cattgaaacc atcgctcatt
     1741 atgctgctgc caaacatatc agcaaagaag tttcttttat cctggatatt ggagggcagg
     1801 atatgaaagc catctttgtg aacgacggag taatcaacag aatcgaaatc aatgaagcct
     1861 gttcctccgg ttgcggttct tttatcagca catttgcgca atcgctggat tacagtgtgg
     1921 aggactttgc caaagcagct tgtttttcgc aagcaccatg tgatctgggt acacgttgca
     1981 cagtcttcat gaactccaaa gtaaaacagg tacttcggga aggagcgtcc gtagcggata
     2041 tagctgccgg attatcttat tccgttgtta aaaactgcct gtataaggtt ctgcaattaa
     2101 aagacacgga gatacttggc gaccatattg tcgtgcaggg aggaaccatg cgtaatgatt
     2161 ccatcgtacg aagtctggaa aagctgaccg ggaaacaaac cttccgcagt aactgtcctg
     2221 aattgatggg tgctcttggt tgtgccctat atgccaaaca aatcgaaaat gcccgggtga
     2281 cggagctgaa cgagatgctc caatgggcgc aatatacttc caagcaactt caatgccggg
     2341 ggtgcgagaa ccagtgcgcc attatgcgat atacgttcaa caatgacaac cattacttct
     2401 ccggcaaccg atgtgaaaag gtattttcca ataaaggaag ccatgcggac aaaggcatca
     2461 atacgtatga taaaaagctg gaattattat tcgaccggag cgcagacatt ccacaacccc
     2521 tatttaccat aggcattcca cgtattctga atatgtatga ggaataccct ttctggcata
     2581 ccttatttac tgcttgcggc attcaggtac agttgtcggc cccctctaca ttcagcaaat
     2641 acgagactgc tgccggcatg gtgatgtctg ataacatctg cttccctgcc aaacttgtgc
     2701 acagtcatat ccgtaacctg acactgcaaa acgtcaaccg gatatttatg ccgttcgttg
     2761 ttttcgagaa aaaggataag caacagcaaa acagctataa ttgccccatc gtttccggtt
     2821 attcggaagt catcaaaagt gtacaggaag agaatatacc gatagatgct ccgactatca
     2881 ccttcaaaga tgaagcattg ctgtataaac aatgctatga atatctgaaa tctttgggta
     2941 tcagggatga ggtatgcaaa aatgccttct cacgcgcatt acaggaacaa tatgctttcg
     3001 aagaaaaaat tgctgcctat aatcaggaag ttctgaacga gggacgggaa aaacacaaac
     3061 tgattatctt actcgccgga cgcccttacc atagcgatcc actgatacag cacaaggttt
     3121 cggacatgat tgctgcgatg ggagtttatg tgataactga tgatattgtc cgccagcagg
     3181 agatttcatt ggaaaagact cattacctct ctcaatgggc atttaccaac cgtattctga
     3241 aagcaaccaa gtgggcggca atgcaagagg gtgacataca atatatgcag atgacttctt
     3301 ttggctgcgg tccggatgct ttccttatag atgaagtacg taacttgctg aaacgatacg
     3361 gcaagaatct tactttgctc aagatagacg atgttaataa tatcggctcc atcaaattac
     3421 gagtccgctc gttggtggaa agcttgaatt tcagtttgaa acattctcaa gcgaaagatc
     3481 ccgagccatt tgtttcaact gctcctttca ccaagaaaga caaaaagaag aaaatcctcg
     3541 ctccattctt tacgcctttc atttcaccac tcattccatc catcatgaaa gtggccggat
     3601 atgaaatgga aacgttaccg ttgagcgaca ccgcatcttg cgactgggga ttgaaatact
     3661 ccaataatga ggtatgttat ccggcaacat tgattgtcgg cgacatagta aaagccttta
     3721 aaagcggacg ttatgatcct gccaatacgt gcgttgccat cacacaaacc ggtggacaat
     3781 gtcgtgccag caactatatt tcactgataa aaaaggcatt gattgagaat ggatacacca
     3841 atacaccggt ggtatcactt gctttcggca gtggtattga aaatgagcaa tccggtttta
     3901 aagtaaactg gctcaaggtt ttgccaatca tacttgcgtc tgtgttatac agtgattgta
     3961 tcgctaaatt ctattatgca gccgtcgttc gtgagaaaga acggggacag gccgcccgtc
     4021 tgcgcgatct ttatctggat acagcacagc ccatcatcca gaaaaataaa ccggaggact
     4081 tgttgagtta cttatatctc gctgccagag acttcaacaa aatctgtgaa cagagatcct
     4141 gtcacaaagt aggtattgtt ggagaaatat tcctgaagtt caaccctttt gctcaaaagg
     4201 acgtcacctc atggctcatc aaccagaaga tcgaagtgat tccgccactg ataagtgatt
     4261 tcttcatgca gggatttgtc aacctgaaag tgagacagaa ccaacacttg cagcgaaaac
     4321 tcactcccga ctttgttatc gactggttat acaagaaagt ccagaagcag attaataaag
     4381 tgaatgaaat cggcaaaaat ttcaattact tcacgccgtt cgaatcaatc tttgaaaagg
     4441 cagaaaaagc taaacaagtc atctccccgg gcacccagtt tggagagggt tggctgatat
     4501 ccggtgaaat actatccttt gcttcccaag gagtcaatca tgtcattagc ttgcaacctt
     4561 tcggatgcat tgccaatcac attgtagaaa aaggaataga aaaacgtata aaatcgctgt
     4621 gccctcaaat gaatatacta tctttagact ttgacagtag cgtcagcgat gtgaacataa
     4681 caaacagatt attacttttt attgacaaca taaacaacta aaattatggc tacaacggaa
     4741 catagcgatt tagagcagca aataataaaa actgcccaac aactttttat agaaaaagga
     4801 tttgtggaga ccagcatgag tgatattgct gcaacggtag gaatcaaccg gcccacttta
     4861 cattattatt tcagaaccaa agataagatg ttcaaagccg tgttcggctc gatagtcatg
     4921 tctttaatgc cgaaaataca ggatatagta aaacaggata tcccatttat ggagcgacta
     4981 agcatgattc tggatgagta tattgagctt ttcacaagta atccctgtct tcccagattc
     5041 atttgcggag aaatacagcg tgatgtaaac cacttgcttg ccactgcaaa agaacttcag
     5101 ttcggagatg ctttatccca agtgaaagac agtatactga cagcaatgga agacggtaaa
     5161 cttaaaaagg ttccgataca tattgtattt ctgactgtct acagcctatt gagctatcca
     5221 tttcttgcca agaacctgat agtatcttta ttcttggaag atgaagccgc ttttgctgac
     5281 ttcctgcaag aatggaaaca aaacatcatc aaccaattgc agaacttatt gtgttatgag
     5341 gaatagtata gatactatat aagga
//
