LOCUS       c00519_gnlPro..         3996 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KELFKKGH_519.
ACCESSION   c00519_gnlPro..
VERSION     c00519_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:19:50
            Original ID  :: gnl|Prokka|KELFKKGH_519
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3996
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..2217
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg519_1"
                     /sec_met_domain="Gly_radical (E-value: 2.1e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNR
                     CLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGI
                     NVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYAR
                     GRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMKVMGE
                     YYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSKGTIT
                     ESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRF
                     LQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYG
                     IACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLNFEE
                     VMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIAL
                     DSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLP
                     VYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSV
                     AKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLNREML
                     YDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     protocluster    1..3996
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2961]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2961
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3996
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3996
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2233..2961
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg519_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2989..3432)
                     /locus_tag="ctg519_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVNELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3419..3586)
                     /locus_tag="ctg519_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(3607..3996)
                     /locus_tag="ctg519_5"
                     /transl_table=11
                     /translation="LCEGITSRSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSL
                     QIGLNSEGAVSVKEFSSSLITAAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNE
                     GEDDDKGSGGGSDGGGSGEAPDPAA"
ORIGIN
        1 aagatcttta aagacggtct ttggagcaca gaaatcaacg taagagattt cgttagtcac
       61 aacatcactc cgtattatgg agatgcttct ttcctcgaag gacctaccga acgcactaaa
      121 gctgtatgga accgctgcct cgaagcactg gcagaagaaa gagccaataa cggagtacgc
      181 tcactggata atgtcaccgt ctcaaccatc acttctcata aagccggata tatcgacaaa
      241 gaaaacgaac tgatcgtcgg tctgcaaaca gacgaacttc tgaaacgtgc aatcaaacct
      301 ttcggaggta tcaacgtggt gagcaaagcc tgccacgaaa acggcgtgga agtggacgac
      361 cgtgtaaaag atatattcac tcactaccgc aagacgcaca atgacggagt atttgacgta
      421 tataccgaag aaatccgttc gttccgttct ctgggattcc tcaccggact tcctgacaac
      481 tatgcccgcg gacgtatcat cggcgactac cgccgtatgg ctctctatgg tatcgaccga
      541 ctgattgagg caaaaaaaga agatttgcgt aaccttaccg gtccgatgac cgaagcccgc
      601 atccgcctgc gcgaagaagt ggcagagcaa atcaaggcat tgaaggacat gaaggtaatg
      661 ggcgaatact acggactcga cttgagccgt cctgcctaca ccgcacaaga agcagtacaa
      721 tgggtgtaca tggcttacct tgctgccgta aaagaacaag acggtgctgc catgtcactg
      781 ggtaacgtct cttcgttcct cgacatttat atggaatatg aattgagcaa aggaactatc
      841 acagaatctt tcgcacagga gctgatcgac cagtttgtca tcaaactgcg tatggtgcgc
      901 cacctgcgta tgcaatctta caacgacatc tttgccggcg acccgacatg ggtaaccgaa
      961 tccctgggcg gacgcctcaa cgacggacgc accaaggtga caaagacttc tttccgcttc
     1021 ctgcaaacac tctacaacct cggcccttca ccggaaccga acctgactgt attgtggagc
     1081 ccggaactgc cggaaggatt caaagaattc tgcgcaaaag tatctatcga cacttcttcc
     1141 atccaatatg aaaacgacga cctgatgcgt gaagtgcgcc agtctgatga ctacggaatc
     1201 gcgtgctgcg tatcttatca ggaaatcgga aaacaaatcc agttcttcgg cgcacgttgc
     1261 aacctggcta aagccctgct gcttgccatc aacggcggac gttgcgaaaa caccggtacg
     1321 gtaatggtga aaaacatccc cgtactaacc agcgacacac tgaacttcga agaagtaatg
     1381 agcaactaca agaaggtatt gacagaaatc gcccgtgttt acaacgaagc gatgaacatc
     1441 atccattata tgcatgataa gtattactac gaaaaagccc aaatggctct tgtagatacc
     1501 aacccacgca tcaaccttgc ttacggtgta gccggacttt ctatcgccct cgattcactg
     1561 tcggccatca aatatgccaa agtaaccgca cgccgcaacg acatcggtct gacagaaggt
     1621 ttcgatattc aaggcgaatt cccttgcttc ggtaatgaca acgacaaggt agaccacctg
     1681 ggtgttgacc tggtctattt cttcagcgaa gaattgaaga aacttccggt ttacaaaaat
     1741 gcccgtccta ctctgtcttt gctcaccatc acttccaacg tgatgtatgg caagaagaca
     1801 ggtgctactc ccgacggccg cgccaaaggt gtcgcttttg ctccgggagc caaccctatg
     1861 cacggacgtg acaagaacgg tgccatcgcc tctttgagtt ccgtagcgaa attacgttac
     1921 cgcgactcac aggacggtat cagcaacacc ttctccatcg ttccgaaatc actgggagct
     1981 actgaagaag atcgcgtaga aaatctggtg acaatgatgg atggttactt caccaaaggc
     2041 gctcaccacc tgaacgtgaa cgtactgaac cgtgaaatgc tttacgacgc catggaacat
     2101 ccggaaaaat atccgcaact caccatccgt gtttccggtt atgccgtaaa cttcgtgaag
     2161 ttgagccgcg aacatcaatt ggaagttatc agccgtagct tccacgaacg tatgtaatca
     2221 gatctactca atatgacgat aaacgtacat tcatacgaaa gtatgggaac attcgacggg
     2281 ccgggcttac ggctcgtcgt ttttcttcaa ggatgcaatt tccgctgcct gtattgtgcc
     2341 aatcccgaca cgatagccgg aaaaggaggt acacctaccc caccggaaga aatcgtccgc
     2401 atggctatga gccaacgtcc ttttttcggg aaacgcggag gagtcacttt ctccggagga
     2461 gaacctacgt ttcaggccaa agcactagtc cctttagtgc gtgaactgaa agaaaaggga
     2521 atacatgtat gcatagacag caacggcggt atctggaacg aagaagtaga agaacttttc
     2581 aaactgacgg atcttgtatt gctggatatt aaagagttca atccggcacg ccatcaagca
     2641 ctgaccggaa gaagtaacga gcaaaccatc cgcacggcag catggctgga agaaaacgag
     2701 aaacctttct ggctgcgtta tgtattagtg cccggataca gcgatttcga agaggatatc
     2761 cgccgcttgg gagaggctct gggaaaatat aagatggtgc aacgagtgga aatcctgcct
     2821 tatcatacat taggcgtaca taagtatgaa gcgatggagc aggaatacaa gctgaaagac
     2881 gtaaaagaaa atacccccga acaacttgaa aaagccgcgg aggtatttaa agagtatttc
     2941 actactgtgg tggtcaatta accaccacag taataaatag gataaaaatc accatgtctc
     3001 tgctttcacc tcgaaacagg gacactcttt gatccgctcc caagggtcaa caattccatt
     3061 ataattggtg tcaggactca agtcccgatg accgaccacc tttgcatccg gataacgctg
     3121 caacaactcg tttaccagac gatgcatcgc ctctttctgg cgggaagtac gcgtatctgc
     3181 tgcctttccg ttctcatcca gcccgccttc atagcacact cctaaactaa tcgcattaaa
     3241 accgcgtgca tgagcaccca ccgtatcttc atccctcatc ggctctatct gtcccgactt
     3301 gcggatataa taatgatacc cccaacaaga aaagccccga aagcgatggg cagtgtccac
     3361 atccttcgcc gtgaagtcgc gatcagcacg ggtagcagag cagtgaatca caatcagact
     3421 aatctttctc atcgtccttc tccctttccg tcgttttcac attacacact tccttatggg
     3481 tagcattgaa gcgcacctct ctcacaattc tgttccgctt tctttttccg aagaaaggca
     3541 caaggaacat aattacatcc aaaatcttag ttacaatttc tttcatttga ttcaatatta
     3601 aagttatcat gctgccggat ccggagcttc tccgcttcca ccaccgtctg aaccgccacc
     3661 ggaacctttg tcatcatctt ctccttcatt gccgctaccg cccgtttggg ccaccgcacg
     3721 cgtcgttacc tgctggtagc taagaatctt cgtgatgtcc gccaacgtct ttccgggacg
     3781 aaaaataata tgggcagccg taatcagcga acttgaaaac tccttgaccg acacagctcc
     3841 ttcactgttc aacccgatct gcaaactgcc caaatcaccc aaccggacta ttcttcccgc
     3901 tttcaacgca cgcttcgcct cataaagaat acccgaaatg gcggcacgca catctgtctc
     3961 cgtacaggta gaccggctgg tgattccctc gcacaa
//
