LOCUS       c00065_gnlPro..         7896 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FKLHIPFH_65.
ACCESSION   c00065_gnlPro..
VERSION     c00065_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 14:20:40
            Original ID  :: gnl|Prokka|FKLHIPFH_65
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7896
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7896
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4612:7583]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4613..7583
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7896
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7896
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             138..491
                     /locus_tag="ctg65_1"
                     /transl_table=11
                     /translation="MEKKRKIRTYGGYFEAFMETLTEKEQDKIQYGLLLLKTQERLSAK
                     FVKFIRDGVFELRTEYNNNIYRVFFIFDDGNIVVLFNGFQKKSQKTPGHEIDKAVKIKE
                     EYYADKQSSNSRL"
     CDS             457..759
                     /locus_tag="ctg65_2"
                     /transl_table=11
                     /translation="MQINNHQIVDYDAVLDAKFGAEGTPERAEAEEKAYAFYTGQIIED
                     ARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(829..2943)
                     /locus_tag="ctg65_3"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     NYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(3030..3260)
                     /locus_tag="ctg65_4"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3454..3957
                     /locus_tag="ctg65_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             4017..4184
                     /locus_tag="ctg65_6"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             4171..4620
                     /locus_tag="ctg65_7"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYICK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4613..5341)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg65_8"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5355..7583)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg65_9"
                     /sec_met_domain="Gly_radical (E-value: 5.6e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tagaaataaa gaaataaaat agaaaaagtt tcaattcctc tctcactcct ctcacttttg
       61 ggggtgaaga tgatgaaaaa taatataaaa cgaggaaatg tgttgtatag ttgaacaata
      121 ttccttatat ttgcgttatg gaaaagaaac gtaagatccg tacatacgga ggttactttg
      181 aggctttcat ggagacttta accgaaaagg aacaagataa gattcaatac ggattactct
      241 tgttgaaaac acaagaaaga ctctccgcta aatttgtgaa gtttattcgg gatggagttt
      301 ttgaacttcg cacagaatat aataacaata tataccgggt atttttcata tttgatgatg
      361 gtaatattgt tgtgttgttc aatggctttc aaaagaagtc acaaaaaaca cctggtcatg
      421 aaattgataa agcagttaaa ataaaggagg aatattatgc agataaacaa tcatcaaata
      481 gtagattatg atgctgtcct tgatgccaaa tttggtgctg aaggtactcc cgaacgtgca
      541 gaggcagaag aaaaggccta cgccttttat acaggacaga ttatcgagga cgcaagaaaa
      601 aaggctaaaa tcactcaggc tgaattagcc cggcgtatcg gttctgaccg ttcttatatt
      661 tcaagagtag aaagtggtca gacagagcct aaagtatcta ctttctatcg tataatgaat
      721 gcgttaggct gtaaaatcga attttcaatg attttgtaaa aacaataaat gcagagtatc
      781 tgtcaagatg actctgcatt ttgctatatt cacaaggctt tcccgcttct attcatacag
      841 cctgtttgat tttacctgct cgatatctat aataaatacg gcatatcggc gcccgttatt
      901 tcctttttta gattcaaaat ggtgcttagt catcaccttc cccaactgta tttttgatcc
      961 gatggaatac tgataaccgg tacgctcacg tattagttcc gcaatctcac tgacagtcag
     1021 ataataaacc ttctcgaaac gttccggctt gcggatatgc gtaagaacca tttcttcttc
     1081 cggtgaatga aagatgaaag cctcgttatt ctcttcaagc cgttcattgt ccgcttgcgt
     1141 gaaccagtat tgatatcccg gcttattcag cagatatttg atctgagcat agagctgcgc
     1201 ataattgatg gtaataaatt ggatggaata aggatgaaaa caaaggaaac gccggttgcc
     1261 tgtcgtgtcg tgaagcactt cctgataatt gcaggttcct gcaaaagagg ccgtatgagg
     1321 gaagttttgc gaacggcgtg cataaggcag acggaaactg atcactttgc gcgttaccaa
     1381 atctttgaat gcattcagtt cccggccgct catcccttca aactcgtcga gattgatcag
     1441 catggcctgc gttattttgg ctaaatcatc tttattgttc gaattgatta gtccggtaga
     1501 gagataagca cgcagttcgg gaggcaggag attattgata aaggtagtct ttcctatgtt
     1561 ctgtatctcg ctgcatagca gaagtaccgt gtgattgaca atattctcct gagtagccgc
     1621 tgcgcacatc gccaccagat aacgttccag gcattccgcc caaaactcct gatgactcgt
     1681 ctggacacta tccgccagga tacggatata atcggttccg tcccattcgg gcagtaattc
     1741 aaaatactcg cgaatgggat gatacgactg gctgaaatcg gagtaaatca gattttcgat
     1801 tgttttgaca gtgcagggat atcccagttc gttgatttct atccagatac tgttttccat
     1861 catctcatcc aagatgcgga aaggctccgg tttctcggtg tcgggatgcc ttctgcgata
     1921 ttccatgata tgcagcactt cgttgtagcg tgtttcgtag tgctcgctga tatattgctc
     1981 tatccgtaat attcgtttct gcgtgccatt cagtttacag gtattaaatt cgtcggtatg
     2041 agcgtaggcg ctattgatca aagagactgt ctcgtctgcc ggaaagtctg taaactggct
     2101 tttgataaaa gcggatgctt cctcctgcgg aatgccgtag cgattgaagg tacatgagag
     2161 atggtgcaga taattgttcc ggttgcctgc tatgtatttt tcggacttgt tgtgatagta
     2221 ggttaaagtc agcaccagag aagcgttggc ggaatgggcg ttgagcgctg tgtcttcatt
     2281 cgtcgggggg ccgtccggag gagtctgttg tttactgccg gagagatttt tacgatttga
     2341 aaccttgtag gatgcctgag gttgatctac aagaaaagcg tcagcgttcg gattgaaata
     2401 aatctccgga tcgtaagaga acagacaagt gcgtgctaca tcctggccgg aggtgtcgat
     2461 ttctatctga cagagttcgg tgtaaaaaga aacgagtctg gtgtaagctg catgatggaa
     2521 gtcttctatt tcctgaaggg tttcgggcaa tgtctcatcc ggatggcatg cgcgtactat
     2581 gattttcacg cccatccctt tgggactgat aaaggcgatg cgggtgtaat tgcatctctt
     2641 gatgataggg aggatgcgtg ccaattcttc tttgctgagg tggtcgatgt ctatcacgat
     2701 gtgtcccaga tatttgacga ggttcacttt ggtacgcctt cctcggtagc tggcggagaa
     2761 ggcgattgcc ggcaattctt ttttcttctc attggcagcc ttttctcctt gctctgcaaa
     2821 gatgcggcgt atttcttcga ctttttcccg gtaggtaaaa ctgccgatag tggacactat
     2881 ttcttcttcg gtcatctctg ctattgcgta gtagaaacct tgaaatatgg tgaaatgttt
     2941 catttttatt ttgattattg tttgtcgatg caaaggaact tctttgcgga aatgtgtaaa
     3001 ggaactttgt ataaaatggc aataagttac tacggttcgc ccaaaaagtg tatgatagtt
     3061 gccacttgtt tcggcgaaag gagacggttg cgtgcgcggt agccttcctg ttcgagttcg
     3121 tgcaggagag ggaggttgta aataatccaa cgacgaagta tttgtaaagc aactttgagg
     3181 cagacatggg gattgtaaag gtgagccagt tccgctttgg tataggtgcg gataaggaaa
     3241 gaatcttctt tgtaattcat gatgttttct tcttgtttta tgctataaag ttacataaaa
     3301 ttccttgttt ttcaaaggtt tgacttcgtt tttattcgtt gggaatcaag aaacttcgtt
     3361 ggggaatata gcttgtccgg ctatccttat ctttgtgctg ttaacaagaa ggggatgcat
     3421 cctatgtatt ataaacttta aaattatttt attatggcaa tgacagtatc ttattcagtg
     3481 gtgccgcgca agaatcctgc aaaaaagagc gaaccggcaa agtattatgc acaggcgcag
     3541 gcgtcgggtg agctggactt tgaagagttg tgcgaagcga tcaccagtcg ttctacttgt
     3601 acggaaacgg atgtacgtgc ggctatttcg ggtattcttt acgaagtgaa acgtgcgttg
     3661 aaggcaggaa gaattgcgag actgggcgat ttgggcagtt tgcagatagg actcaacagt
     3721 gaaggtgcgg catcggtcaa ggaattttcc ggttcaatga ttaagggggc gcatcttatt
     3781 ttccgccccg gtaagacgct ggcggagttg atgaagattc tgagttatca gcaagtgcag
     3841 actcgtgcgg tggcgcaggc gggtgcaggc gatggcgggg gagaagaccc tgacaagaat
     3901 ccggattccg gtggtgatgg ctccggtgat gaagaggctc cggaccctac ggtataattg
     3961 gcagacgggt caaggtaata acgaattaga ataacttcca ttaatatttt gataagatga
     4021 aagaaatagt aaaccggatt ttagatgtga tcatgtatct gattcctttc ttcggaaaga
     4081 gaaagcggga cagggtggtg agagaagtgc gctatcatac tacttgtaag gaggtgtgca
     4141 aagtgaaaac gacggaaagg gaaaaggaac atgagaaaga ttagtctgat tgtgatccat
     4201 tgttccgcca cccgtgtcga ttgcgacttt acggcaaagg atgtggatac ggctcaccgt
     4261 tatcgtggct tttcgtgctg ggggtatcat tattatatat gcaagtcggg cgagattgag
     4321 ccgatgcggg atgaagatac ggtcggagct catgcacgtg gctataatgc gatcagctta
     4381 ggtgtatgtt atgagggcgg tcttgatgag aatggaaaag ctgcggatac gcgtacaccc
     4441 cgtcagaagg aggcgctaca tcgcttggta cacgaattgt tgcagcgtta tccggaagct
     4501 aaagtagtag ggcatcggga tttaagtccc gacaccaact ataatgggat cgtagatcct
     4561 tgggaacgga ttaaagaatg tccttgcttt gaggtcattg gagaattctc cattaattaa
     4621 ctaccaccgt agtgaaatac tctttgaata cctccgcagc tttttcaagc tgttcggggg
     4681 tattttcttt tacccctttc atcttgtatt cctgtcccat cgcttcgtac ttatgtacgc
     4741 ccagtgtatg gtaaggcaat atttccaccc gttggatcat cttgtatttt ccgagtgctt
     4801 ctcccagtgc gcgtatgtct tcttcaaaat cgctgtatcc gggcactagc acgtagcgta
     4861 gccagaaagg tttctcctgt tcttccagcc aggcggccgt gcggatggtt tgctcgttgc
     4921 ttcttccggt gagcgtctga tggcggttag ggttgaattc ctttatatcc aataacacaa
     4981 gatcggtcag tctgaataat tcttctacgt cttcgttcca aagtcctccg ttgctgtcca
     5041 gacagacatg gatgcctttt tctttcagtt cgcggaccag cgggacgagt gctttcgcct
     5101 gaaacgtcgg ttcgcctccg gagaaagtga ttccccctcg tttcccgaag aaggggcgct
     5161 ggctcattgc catgcggacg atttcttccg gtggggtagg cgtgcctccc tttcctgcta
     5221 tcgtatccgg attggcgcaa tagaggcagc ggaagttgca tccttgaaga aaaacgacga
     5281 gccgtaagcc cggcccgtcg aatgttccca tactttcgta tgaatgtacg tttatcatca
     5341 tatagagtaa tagattacat acgttcgtgg aagctacggc tgataacttc cagttggtgt
     5401 tcgcggctca gcttcacgaa gtttacggca tagccggaaa cgcggatagt cagctgcgga
     5461 tagttttccg gatgctccat ggcatcgtac agcatatcac ggttcagtac gtttacgttc
     5521 aggtggtggg cacctttggt gaagtaaccg tccatcatcg tcacgaggtt ctcgatacgg
     5581 tcttcgtccg tagcacccag tgatttcgga acgatagaga aggtgttgct gataccgtcc
     5641 tgtgagtcac ggtaacgaag tttcgctaca gaactcagag aagcgatcgc accattcttg
     5701 tcgcgtccgt gcatcggatt ggcacccgga gcgaaggcaa cacctttggc acgtccgtcg
     5761 ggagtagcac cggtcttttt gccgtacatc acattggaag tgatagtcag caaggagagg
     5821 gtaggacggg cattcttgta aacaggcaat ttcttcaatt cttcgctgaa gaagtatacc
     5881 aggtctacgc caaggtggtc taccttgtcg ttgtcgttac cgaagcaagg gaattctcct
     5941 tcgatgtcga agccttctgt cagaccgata tcattgcggc gggcagttac tttcgcatat
     6001 ttgatggcag acagtgaatc gagggcgatg gaaagtccgg ctacaccgta agccaggttg
     6061 atgcgcgggt tcgtatctac aagagccatc tgggccttct cgtagtaata cttgtcgtgc
     6121 atatagtgga tgatgttcat cgcttcgtta tatacgcggg cgatttcggt cagcactttt
     6181 ttgtagttgt ccattacttc ttcgaacttc agcgtgtcac tggtcagtac ggggatattc
     6241 ttcaccatga ctgtaccggt attctcgcaa cgtccgccgt tgatggcaag caacagggct
     6301 ttggccaggt tgcaacgggc gccgaagaac tgaatctgct tacctatttc ctggtatgat
     6361 acgcagcagg cgattccgta gtcgtcggac tgacgtactt cgcgcatcag gtcgtcattt
     6421 tcgtactgga tagaagaagt gtcgatagaa acctttgcac agaattcttt gaatccttcc
     6481 ggaagttccg ggctccagag tacggtcagg ttcggttccg gtgaagggcc gaggttgtac
     6541 aatgtctgca ggaaacggaa ggaggtcttt gtcactttgg tacgtccgtc gttgagacgt
     6601 ccgcccagag attcggttac ccaggtcggg tcgcctgcaa agatgtcgtt gtatgattgc
     6661 atacgcaggt ggcggaccat acgcagtttg atgacaaact ggtcgatcag ctcttgcgcg
     6721 aatgattcgg tgatggttcc tttgctcagt tcatattcca gatagatatc gaggaaagaa
     6781 gaaacgttac ccagtgacat ggcggcaccg tcttgttctt tgacggcggc aaggtaagcc
     6841 atatataccc actgtacggc ttcttgtgcc gtgtaagcgg gacggctcag gtcgagaccg
     6901 tagtattcgc ccatcacttt catgtctttc agtgccttga tctgttctgc cacttcttcg
     6961 cgcaggcgga tgcgggcctc tgtcatcgga ccggtgaggt tgtgcaaatc ttccttcttt
     7021 gcttcgatca gacggtcgat gccgtaaaga gccatacggc ggtagtcacc gatgatgcgt
     7081 ccgcgtgcat aattgtcggg aagtccggta aggaatccca gcgaacggaa ggaacggatt
     7141 tcttccgtat atacgtcgaa tactccgtcg ttgtgcgtct tgcggtagtg agtgaaaata
     7201 tcttttacgc ggtcgtccac ttccacgccg ttttcgtgac aggctttgct gactacattg
     7261 atacctccga aaggcttgat ggcacgtttc agaagttcgt ctgtctgcag gccgacgatc
     7321 agttcgtttt ccttgtcgat atatccggcc ttgtgggaag tgatggttga tacggtaaca
     7381 ttatctaatg agcggacacc gttgttttct ctttcttctg ccagtgcttc gaggcagcgg
     7441 ttccatacgg ctttggtacg ttctgtaggt ccttcgagga atgaagcatc tccgtaatac
     7501 ggagtgatgt tatgacttac gaaatctctg acgttgattt cgctgctcca aagaccgtct
     7561 ttaaagatct tgtttaattc cataaaatga taaaattaga ggttgtagtt atcctattct
     7621 ttagagcgca caaagttaca atcatttttc atataatcag cataaatatg ctctatttta
     7681 tgcttttcaa cataaaatac ctattaattg ctatccttag gaaaagatta gattaaattt
     7741 ttagtgaaaa ctcttgcaag tttaaaatat cttcgtacct ttgcaccgct tttaacgaaa
     7801 agcacttctg ataaagaagt tttggagagg tggcagagtg gtcgattgcg gcggtcttga
     7861 aaaccgttgt actgcgaggt acccggggtt cgaatc
//
