LOCUS       c00074_gnlPro..        14981 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JBEIABIM_74.
ACCESSION   c00074_gnlPro..
VERSION     c00074_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:58:08
            Original ID  :: gnl|Prokka|JBEIABIM_74
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14981
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14981
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[369:4981]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      370..4981
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..14981
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14981
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(2..109)
                     /locus_tag="ctg74_1"
                     /transl_table=11
                     /translation="MVYATKLLQKSEMRKNMVMDLIYRGFIYAANYSESH"
     CDS             complement(211..306)
                     /locus_tag="ctg74_2"
                     /transl_table=11
                     /translation="MIQFKGLRQGLNPVGGSAGCQYNMNSHLLSL"
     CDS             370..1356
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_3"
                     /sec_met_domain="HBT (E-value: 2.2e-17, bitscore: 53.9,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEKRIDPAMIPYLNRKAKENGFAIFTYHKDYILTDSPENKHVQE
                     EAELNKMRIIGVENFPEAVDFAPCKCILTSDDENNLVGLENHWKKRLDGVLEAFRSEDY
                     FLEVAPHFINKGNTLAVLMEMLNITTEEVVAIGDGVADVSMLQLAGTGVAMGNARDSVK
                     ACADFTTLPNNMEGVAVAIEKAILATIKPTEVPLDQLNARAKHALMGNLGIQYTYASED
                     RVEATMPVDERTRQPFGILHGGATLALAETVAGLGSMILAKPDEMVVGMQVSGSHMSSA
                     HEGDTVRAVGTIIHKGRSSHVWNVDIFTSTDKLVSSVRVINSILKKK"
     CDS             1353..2450
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg74_4"
                     /sec_met_domain="Chorismate_bind (E-value: 2.9e-54,
                     bitscore: 174.9, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPRDSYEIIDTLIKQGRSFAIWRIPGEDRLHFRMQSAGSPCLLY
                     DIKDLNERSGFVIAPFQVTAERPIVLIQPDCFEIPSESDWDFSREKNEFPSNEIEIPSE
                     SEEKERYAYHFSLFTNPLLKGSQDKLVLSRSKTIWKDPSFSPGKAFFAAEERYIRSYVY
                     LCHTPETGTWMGGTPEILLSGEKGDWQTVALAGTQSLRDGKLPKSWDHKNWREQQLVAS
                     YIRRQLSTLGITPEEKGPYSARAGEVSHLKSDFFFSLPNPEKLGDVLQLLHPTPAVCGL
                     PKEEAYHFIIENEGYDRSYYSGFIGWLDPKGKTDLYVNLRCMNILPQTFTLYAGGGLLA
                     ASQLEDEWQETEDKLDTMRRIVGNL"
     CDS             complement(2491..2697)
                     /locus_tag="ctg74_5"
                     /transl_table=11
                     /translation="VPEVQQTAAGLPPCNASPTAKNPALRSSVTEWQLKNEWFANVCTI
                     GEFRLPGHSTILLTPCSISKATN"
     CDS             2798..4153
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_6"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.1e-07, bitscore:
                     22.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLEL
                     NHHGKGPVHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRM
                     MIAGQMSLIYLFEKKICKLLYKHFTWLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKL
                     VPELVITYGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLTTVIEMGPFEFLEK
                     IAYMLENRQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRY
                     AQLYTVPETVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGN
                     LHNNLRILLLNNGGGEIFQALPGFKMNERTHRYVTASHQTSAEGWATERGFSYSAVHNA
                     EELAAAMSAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQNI"
     CDS             4157..4981
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_7"
                     /sec_met_domain="ECH_1 (E-value: 6.1e-82, bitscore: 265.2,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METKREWTTIKEYEEILFDFYNGIARITINRERYRNAFTPTTTTE
                     MSDAFYICRERPDINVVVLTGAGDTAFCAGGDMNVKGRGGYVGKDGVPRLNVLDVQKQI
                     RSLPKPVIAAVNGFAIGGGHVLHVVCDLTIASENAIFGQTGPRVGSFDAGFGSSYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLDKLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATMLYYMTDEAQEGGKAFLEKRRPRFEDYPKFP"
     CDS             5005..6063
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg74_8"
                     /sec_met_domain="MR_MLE_C (E-value: 3.4e-29, bitscore:
                     92.5, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEP
                     SAFGIGECAPLPDLSCDYGVDYEITLSKACLDLEQKGHVDTESLRHYPSILFGLEMAMR
                     HYEQGSWRHYDTPFSRGQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGAIDF
                     DRELSLLKHIRAHFSAKEIELRVDANGAFLPSEAMDKLKRLAEMDLHSIEQPIRAGQLE
                     EMARLAAESPLPIALDEELIGCNAPDEKRRLLETIHPQYIILKPSLHGGIAGCTEWVHL
                     AETILGSTPEQPKWWVTSALESQIGLNAIAQWCATLGNPLPQGLGTGEIFKDDKHKLIQ
                     RKGDTIWFTNDL"
     CDS             6067..7065
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 53; E-value: 6.9e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg74_9"
                     /sec_met_domain="AMP-binding (E-value: 2.6e-17, bitscore:
                     53.0, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPIQEDLQAFLAEWHDPSPFLEVQTSGSTGTPKRMRVRKDRMLNS
                     ARLTCDYLGLKKGDKALLCMPLRYIAGKMMVVRSLYAGLDLEVCEPSGHPLADWGDTPL
                     RFVAMIPLQVYNTLRVPEERKRLEQTDILIIGGGAIDAALEQEIRQMPNTVYSTYGMTE
                     TLSHIALRRLNGPEASPYYHPFPSVTLSLSPDNTLVIDAPLVCDERLVTNDVARLLPDG
                     SFAIIGRKDNIINSGGIKIQIEEVEERLRPYLDRPYAITAAPDPRLGEAVVLLLASNLR
                     GEMLTEVLASLPKYLRPRHIIKVSEIPQTGSGKIDRAACRELARNIITKTL"
     CDS             7068..9029
                     /locus_tag="ctg74_10"
                     /transl_table=11
                     /translation="MKRILTITAVSLLALSPMYGQDSCRYPQDPTLEKSQAYAGYDCVT
                     LLDSTAVTVQPTGSGAFAVCKAFKVQTSKGAVANRIIKYDYDPLTAHAEFRYATIYRAN
                     GDVINLDVTQACDYAAPARAIYWGARQIMLEVGRLQPGDIVEYEIAKKGFTYALLTAGD
                     DDERFIPPMRGQFYDIVPFWATEPTVRKVYRVSMPMEKELQFQFYQGECTSSMRYEDGR
                     KVYTFASDDILPFAKEPNMVDLFDAAPKLMMSSTPRWEDKSLWFNKVNEDYGSFTAIPE
                     AQKKVDELIKGKKTEMEKIAVLTHWVADNIRYSGISMGKGEGFTLHNLKMNYTDRCGVC
                     KDIAGTLIAFLRMAGFEAYPAMTMAGSRVESIPADHFNHCVAVVKLASGTYMPLDPTWV
                     PFCRELWSSAEQQQNYLPGVPEGSDLRLTPVSAPENHYVRIHADNRLDEKGTLRGQFTI
                     TAEGQSDSNIRRIFTTGWQSDWQNTMEKQLLAVSPNAKLLRVDYGKDPKNYQATPIKIT
                     FSYEIPDYAIIAGDQMLIKPLVMNNLYNQVKSFLRIDTKPENRTYGFKDACSRLVEQDE
                     TIRLPKGYTLLNAPKNDALQSNAADFEGSLSQEGDQNIVLRQKLALKKRVYDAADWKGF
                     RSAVNAYKSYGDYLIIKK"
     CDS             9043..10641
                     /locus_tag="ctg74_11"
                     /transl_table=11
                     /translation="MIKITYKLILLLSLLAMTVTSFAASEAEYGKVSKAWTLHADGSQE
                     YRSSMELTLFTHTAMNSTYGESFIVYNPDFQTLKIHSSYTRQKDGTIVKTPDNAFVEVL
                     PRFAADAPAYNQLKEMVVVHTGLELGATIYLDYSIITKPGYYPALDINERLQETSPVKE
                     CKVSISVPENSPLAWQLLGSDAKASQATRNGAKEVSWTLRNLPASSREAFLPQNQDGIP
                     RLIASSYSSNKEALSTLNKRFNTSGNYESKTFGSYITEKATSDEEKVNAIRNHVVNNMG
                     YCAIPLACTGYSIRNVDTALRSAYGTLAEKTQLLNVLLNAVGIQSEVVAVYPGNLDIDA
                     CGLSTIKNLAVKATVAGKDKYLSAVSLAPSALTARGELDQVLTLNGTSIALQAEPTIIK
                     ENKAVSVSKNEAQNGFAICTLPAITGGIDSWGMSALNSKRSNLFELPSLIKEEVTYTVT
                     PAEGMKLQTSTKEQVISKPFGKVTRTITPRGNTIEVVRTIELDKQQFTPAEYSDVRSLI
                     HEWTNPDNRVLLFSL"
     CDS             10744..13257
                     /locus_tag="ctg74_12"
                     /transl_table=11
                     /translation="MKTLKYLLVTMLLLGVCSLTHSQKLSLGIFPEPQEVTISSQTYAP
                     SHGYTLRGINNPDADAVKLLKEALPFAKSGKSLPLEIKKLKDKTPEMQRSGAYTLTITK
                     KGITIGIVDDNSLFYAAQTLKQLAKYDEGKKILPLCSIKDYPDVLFRGTVEGFYGQPWS
                     HADRIEQIHFYGRIKLNTYIYGPKDDPYHSSPNWRKPYPAEEAEHIKELAKEAAHNKVN
                     FVWAIHPGQDIQWNLTDSMNILSKFEKMYDLGVRSFAVFFDDISGEGARPEKQAGLLNY
                     IHKEFISKKNDVQPLIMCPTEYNRSWAKTDYLDILGTQLDPAIQIMWTGDRVVADITKE
                     GVEWVNSRIRRPAYIWWNFPVSDYCQDHLLMGPAYGLDTQAAGTMTGFVSNPMEYAEAS
                     KVAIFGVGMYTWNIENYDPTQAWKDACDFIMPEASMAFRIFCEHNCDPGPNGHQYRREE
                     SANYVAPIQTFLAGYKKNTFPEQSANLLGTLFAQITASPSMIYSQSPNKRLIEQINPWL
                     IQFEFLGKAGTSALHMAHAWYEKDRSYTWQRYLETSALLDSMKLINRTLNQKAQPKGVK
                     VGSKVLHPFIVDLYRQTGRNLLSTDGIAPDEVKVSIPSIFTNIDQLKSQPCAEGDNTVG
                     YVPLFEVVKVQPNQYLGIGWEIQKEAESFCFNLPKSNQPGRVFEWSADGKSWSLIPHVS
                     TENAKDTIRTIDPKARYIRMRNNSDQQMELYVLGFTATTQQDPQINEALMMYDMNLDTY
                     KNLNPGEIIHIKCDDINAVSFFLSGSNENLVSITGLSQDGEKNIVYQGNVGYIKLNKTM
                     FEDFSSLEITTIGKEPIHIHQIVRE"
     CDS             13292..13411
                     /locus_tag="ctg74_13"
                     /transl_table=11
                     /translation="MEEGDRYFFIYSYNSGVSYFWHISACIFLLKVIPLGCEK"
     CDS             13831..14064
                     /locus_tag="ctg74_14"
                     /transl_table=11
                     /translation="MAVDTHVFRVSNRIGLTNNSKTPLETEKELVKNIPEELIPIAHHW
                     LILHGRYVCLARKPKCEECGLKPWCKYFLKQT"
     CDS             14061..14942
                     /locus_tag="ctg74_15"
                     /transl_table=11
                     /translation="MRIGKKDIMAFIVLFLATIVCVRYFYKNMSDEQFVATVDPYSLVI
                     PTPTAIFAINRPPVFEKMILPMENIRKAFSDHTPAIFLSLIQQNPDLSSFLIAYYPQGD
                     ILYAPMDSHTAERIFKQLDASFTFPAQQREEASVPVRYYPDVDKHFLGCYYHEGIFVVS
                     YNRKLLVETAKKQQMYPAQIIPELADLISKKGKSGAMNLFIQSASLDLQVQMNDSTEWK
                     MKNQWLAMDLFYNEGSLCCFNEQPYEETLENFYPNLCDTITTRINRLFPQIKTTAQVSH
                     DEAVVYFTVCGN"
ORIGIN
        1 aatggctctc ggaataattg gcagcgtaaa tgaaacccct gtaaatcaaa tccattacca
       61 tattcttccg catttcactt ttttgtagta actttgtcgc gtaaaccata aaaataagac
      121 gccatgaagt acaaattatt agtcttagac gtagatggga cgttgcttaa cgacaagaaa
      181 gagataactc cccggaccca tgccgccttg ttaaaggctc agcagatggg agttcatgtt
      241 gtattggcat ccggccgacc caccaacggg gttcaaccct tggcggaggc ccttgaattg
      301 aatcattacg gtggtttcat attatcttat aacggaggtc agatcatcaa tgcccagacc
      361 ggagaattga tgttcgagaa acgaatcgat ccggccatga ttccttattt gaaccggaag
      421 gcgaaggaaa acggtttcgc gatcttcaca tatcacaagg actatatcct aaccgatagc
      481 ccggaaaaca agcatgtcca agaggaggcc gaactcaata agatgcgcat catcggtgtg
      541 gagaatttcc cggaagcggt ggatttcgca ccctgtaagt gtattcttac cagcgatgac
      601 gagaacaatc tagtgggtct cgaaaatcat tggaagaaac gtctggacgg ggtattggaa
      661 gctttccgct ccgaggacta tttcttggag gtagctcctc atttcatcaa taagggaaat
      721 acgttggcgg tattgatgga gatgctgaat atcacgaccg aagaggttgt cgctatcggt
      781 gatggcgtgg ccgacgtatc catgcttcaa ttagccggta ccggcgtggc gatgggtaac
      841 gcacgcgatt ccgtaaaggc ttgtgccgat ttcacgacct tacccaataa tatggaaggt
      901 gtggcggtag cgatcgagaa agcgatcctt gccacgatca aacccacaga ggttccgtta
      961 gatcaactaa acgcacgggc caaacatgct ttaatgggta atctaggcat tcaatatacc
     1021 tatgcgtcag aggatcgtgt ggaagcgacc atgcctgtag acgaacggac ccgccagcct
     1081 ttcggtatct tgcacggagg cgctacactg gctctggcag agaccgtggc cggactaggt
     1141 tccatgattc tggcgaaacc ggacgagatg gtagtcggca tgcaagttag cggcagccat
     1201 atgtcgtccg cccacgaggg agacacggta cgtgccgtcg ggacgattat ccacaaaggc
     1261 cgttcatccc atgtgtggaa cgtagacata ttcacctcta ccgacaaact ggtttcctct
     1321 gtacgggtaa tcaatagtat actaaagaag aaatgattcc aagagattct tacgaaataa
     1381 tagacacgtt gatcaagcaa ggccggagtt tcgctatttg gcgtattccc ggtgaggata
     1441 ggctccattt caggatgcaa agtgccggct ctccttgcct attatacgac ataaaagact
     1501 taaatgaacg gagcggtttt gtaatcgctc cgtttcaggt gacagcggaa cgacctatcg
     1561 ttttaatcca accggattgc tttgaaatcc cctccgaatc cgattgggat ttctctcgag
     1621 agaaaaacga attcccttcg aatgaaatcg aaatcccgtc cgaatccgag gagaaggaac
     1681 gatacgctta ccatttctcc ctgtttacca accctttgct aaaaggaagc caagataaac
     1741 tagtcctatc ccgtagcaaa acgatctgga aagatccctc tttctcaccc ggcaaggcat
     1801 tcttcgccgc ggaagaacga tatatccgct cctatgtcta cctttgccat accccggaaa
     1861 ccggaacttg gatgggtggg acaccggaaa tcttgttatc cggcgagaaa ggtgactggc
     1921 aaacggtagc tttggccgga acccaatcct tacgagacgg gaaattaccg aaaagctggg
     1981 accataagaa ttggagagaa cagcaattag tcgcctccta tatacgccgc cagctgtcca
     2041 ccttgggtat tactcccgag gagaaggggc cttattcagc ccgggccgga gaggtttctc
     2101 atttgaaaag tgatttcttc ttctccttgc cgaatcccga gaagctgggt gatgtccttc
     2161 aactattaca ccctactcca gccgtctgcg gtcttcccaa ggaggaggct tatcatttca
     2221 tcatcgaaaa tgaggggtac gatcgtagct attactccgg tttcatcggt tggctggatc
     2281 ccaaaggcaa aacagatctg tacgtaaacc tccgttgcat gaatatcctc ccccaaacct
     2341 ttacgctgta tgccggaggc ggtctgttgg cggcctcgca gctagaggat gagtggcagg
     2401 agacagagga taaattagat acaatgcgga gaatagtggg taatttataa ttttatatca
     2461 tataatgtat agcgataaaa agaacatcct tcaattggta gccttgctga tagagcacgg
     2521 cgtaagtaat atcgtgctat gtccgggaag ccggaactct ccgattgtgc acacattggc
     2581 aaaccattcg ttcttcaatt gccattccgt gacggatgaa cgaagcgcgg gattctttgc
     2641 cgtagggcta gcgttgcacg gaggtaagcc ggcggctgtt tgctgtacct cgggcacggc
     2701 cttgctgaat atacatccgg cggtagcgga ggctttctat cagaaagttc cgcttgtcgt
     2761 gatatccgcc gaccgccccg ccgcatggat cgggcaaatg gacggacaga ccttgccgca
     2821 acccggggtg ttcggctcgc tggtaaagaa gtcggtgcag ctaccggaaa tacatacgga
     2881 tcaagatgaa tggtattgca atcgattgat caacgaggcg ttgttggaac tgaaccatca
     2941 cggcaaagga ccggtgcata tcaacgttcc gatctccgag cctttatttc agttcacgac
     3001 acccgaattg ccgaaggtac gggtaatcac ccgttatcaa ggcttgaacg tgtacgaccg
     3061 gaaatatgat gacctgatcg agcgcttgaa caaatatagc aagcggatga tgatagcggg
     3121 acaaatgagc ttgatctatc tcttcgagaa gaaaatatgc aagttactat acaagcattt
     3181 cacttggctc tccgagcaca tcggcaaccg tatcgtaccg ggattaccga tcaagaattt
     3241 cgacgctatc ctgtatgcta ccccggagga ggaaaaagag aagctagttc ccgagctcgt
     3301 aatcacctac ggtggccata ttgtctccaa gcggctgaaa gagtttttgc ggaaacaccc
     3361 gcctaaggag cattggcatg tctcattgga tggagaggta gcggatttat tcggtagcct
     3421 gaccacggtg atcgagatgg gccctttcga gtttctggag aaaatcgctt atatgctgga
     3481 gaataggcag attgaatatc cgaaggcttg ggaatacaaa tcccggaacc tgccggagcc
     3541 ggagttcgct tactccgaaa tggcggcgat cggtggtttg atacgctcgc ttccggctga
     3601 atcggccttg catctgggga atagctccac cgtacgttac gcgcaattgt acaccgtacc
     3661 cgagacggtt gaggtttgtt gtaaccgagg tacgagcggg atcgaaggct ccttgtctac
     3721 ggccatcgga tacgcctcgg cctcgcaaaa actaaacttt atcgttatag gcgatcttag
     3781 tttcttctat gatatgaatg cgttgtggaa cggtaacttg cacaataacc ttcgcatcct
     3841 gctcttgaac aacggaggtg gcgagatctt ccaagccttg ccgggattca agatgaacga
     3901 gcgtacgcac cgttacgtta ccgcctccca ccagacctca gcggagggat gggccacgga
     3961 aaggggattc tcctactccg ctgtccataa tgccgaggag ttggcagcgg cgatgagcgc
     4021 ctttacccaa acggagcaac cgagcgaaca tcccctattc atggaggttt tcacggacaa
     4081 agcggaagac gtacgattgc tcaaggaata ttatcatcaa ctaaaaaatt catcccaagc
     4141 acaaaacata taatatatgg aaacgaaaag agaatggaca accatcaagg aatatgagga
     4201 gatcttattt gatttctaca acggcatcgc ccgcattacg atcaaccggg aacggtatcg
     4261 caacgctttt accccgacta cgaccacgga gatgagcgac gccttttaca tctgccggga
     4321 acgcccggac attaacgtag tcgttctgac aggagccgga gatacggcct tttgcgcggg
     4381 aggcgatatg aacgtgaaag gacgtggcgg ctacgtgggt aaagacggcg tacctcgctt
     4441 gaacgtattg gacgtacaaa agcagatccg ctctctcccg aaacccgtca tcgcggccgt
     4501 gaatggtttc gctatcggcg gcggacacgt attgcacgtg gtttgcgacc ttacgatcgc
     4561 ttccgagaac gctatcttcg gacagactgg tcctcgtgtg ggtagcttcg acgccgggtt
     4621 cggctcttct tacctcgccc gtgtggtcgg acagaaaaag gcccgtgaga tctggttcct
     4681 atgccgcaag tacaatgcgc aggaggcttt ggatatgggt ttggtaaata aggtagtccc
     4741 cttggataaa ttagaggacg agtacgttca atgggccgag gagatgatgt tgcttagccc
     4801 gttggctttg cgcatgatca aggcgggctt aaacgccgag ttggacggac aggccggtat
     4861 acaggagttg gccggagacg ctaccatgct ttactatatg acggacgagg cgcaggaagg
     4921 cggcaaggca ttcttggaga aacgccgtcc acgcttcgag gattatccga agttcccgta
     4981 aaaggaattg aaaattgaga atgaatggtg aaggagaggt tttataagac agaggtggtt
     5041 ccccggtgct tgactttcaa gcgaccggca ggaacctcac ggggtgttta cacgacccgg
     5101 aaattgtggg aggtccgaat ccgcaaggaa gacgaaccct cggcttttgg catcggtgaa
     5161 tgtgcccccc tgccggatct cagttgcgat tatggagtgg attacgagat cacgttatcc
     5221 aaggcttgcc tcgatctgga acagaagggg catgttgaca ccgaatcttt gcgccattac
     5281 ccatccatcc ttttcggatt ggaaatggcc atgcgtcatt atgaacaggg tagttggcgg
     5341 cattacgaca cgcccttctc gcgagggcag gcgggtatcc ccattaacgg actgatctgg
     5401 atgggcgatt ataagtacat gctggaacag gtagaggaaa agatgaagga gggctttcgc
     5461 tgcgtcaagc tgaagatcgg cgctatcgat ttcgaccggg aactctcttt attgaaacac
     5521 atccgtgcgc atttctctgc caaggaaata gagcttcggg tagatgccaa cggcgctttc
     5581 ctcccctcgg aggcgatgga caaactgaag cggctcgccg agatggactt gcattccatc
     5641 gagcagccta tccgtgccgg gcagttggaa gagatggcac gccttgcggc agagtcaccc
     5701 cttcccatcg ctctcgacga ggaactgatc ggctgtaacg ccccggacga gaaacggcgg
     5761 ttgttggaaa cgatccatcc ccaatacatt atattaaagc cttccctcca cggaggtatc
     5821 gccggatgca cggaatgggt tcacttagcg gaaactatct tagggagtac ccctgaacaa
     5881 cctaaatggt gggtcacctc cgctctggaa tcgcaaatcg gactgaacgc tatcgcccaa
     5941 tggtgcgcca cgctaggtaa cccgctaccg caaggtctgg gaaccggcga gattttcaag
     6001 gacgataaac ataagttgat ccaacgaaaa ggagacacca tctggttcac caacgacctt
     6061 tgacctatgc cgatacaaga agatttacag gcattcttag ctgaatggca cgatccctcc
     6121 ccttttctgg aagtccagac ctctggctcc accggtacgc ccaagcggat gcgagtacgc
     6181 aaggaccgga tgctgaacag cgcccggctt acctgcgatt atctggggct taagaagggc
     6241 gacaaggcct tgttatgtat gcccctacgc tatatagccg gaaaaatgat ggtggtccgc
     6301 tccctatacg ccggactgga tctggaggtg tgcgaaccct ccggacaccc gttggcggat
     6361 tggggggata cgcccctgcg ctttgttgcc atgatcccct tgcaggttta caatacgttg
     6421 cgagtacccg aggaacgtaa gcggctggag caaacggata tcctgattat cggcggcggg
     6481 gcgatcgacg cggccttgga acaggagata cggcagatgc caaacacggt ttattccacc
     6541 tacggaatga cggagacgct gtcccacatc gccctgcgcc ggctgaacgg gccggaagcc
     6601 tcgccctatt accatccctt cccctcggtg acgttatcct tgtcaccgga caataccttg
     6661 gtgatagacg ccccattggt ttgcgacgag cggctggtga cgaacgatgt cgcccgcctg
     6721 ctgccggacg ggagtttcgc cattatcgga aggaaagata atatcatcaa tagcggaggt
     6781 atcaagatcc agatcgagga ggtggaagag cggctacgac cctacctcga ccgcccctat
     6841 gccatcaccg ccgcccccga ccctcggttg ggagaggcgg tcgtgctact gcttgcctca
     6901 aacctacgag gtgagatgct aacagaggtt ctcgccagcc tgcccaagta cctccgtccg
     6961 aggcacatca taaaggtaag cgagatcccc caaaccggaa gcggcaagat cgaccgtgcc
     7021 gcctgccggg agctggcgag aaatatcatt acaaaaacac tataaacatg aaaagaattc
     7081 taacaatcac cgcggtctcc ctattggcct tatcgcctat gtacgggcag gattcttgcc
     7141 gatacccgca agatcctacc ttggagaaat cgcaagccta tgcaggatac gactgcgtca
     7201 ccttgctgga tagcaccgcc gtaacggtac aacctaccgg ctcgggggct ttcgccgtat
     7261 gcaaggcttt taaggtacag acttccaaag gagccgtcgc caaccgtatc atcaaatacg
     7321 attacgaccc gctgacggcg cacgccgagt ttcgttacgc cacgatctac cgtgcgaacg
     7381 gcgacgtgat caatctcgac gtaacccaag cttgcgacta cgccgccccc gcacgtgcca
     7441 tctactgggg cgcccggcag atcatgctgg aagtgggccg cttgcaaccg ggagatatcg
     7501 tggagtacga gatcgccaag aaaggtttca cctacgcctt gctgacggcg ggtgacgacg
     7561 acgagcgttt catccctcct atgcgaggac agttttacga tatcgtcccc ttctgggcta
     7621 ccgagcctac cgtgcgcaag gtgtaccggg tatccatgcc gatggagaaa gagttgcaat
     7681 tccagttcta ccaaggcgaa tgtacctctt cgatgcgata cgaggacgga cggaaagtat
     7741 ataccttcgc ctccgacgat atccttcctt tcgccaagga accgaacatg gtagacctat
     7801 tcgacgctgc cccgaagctc atgatgtcca gcactccccg ctgggaggat aaatcccttt
     7861 ggttcaataa ggttaacgag gattacggaa gctttactgc gatcccggaa gcccagaaaa
     7921 aggtagacga gctgatcaaa ggcaagaaaa cggaaatgga gaagatagcc gtgctgaccc
     7981 actgggtggc cgataacatc cgttactccg gaatctcgat gggtaaaggc gagggtttca
     8041 cgcttcacaa cctgaaaatg aattataccg atcgttgcgg tgtctgcaag gatatcgccg
     8101 gtacgttgat cgccttcctc cgcatggccg gcttcgaggc ttacccggcg atgacaatgg
     8161 cgggaagccg tgtagaaagt atccccgccg accatttcaa ccattgcgtg gcggtggtaa
     8221 aactggcgag cggtacgtat atgcctctcg atccgacttg ggttcctttc tgccgggaat
     8281 tatggagtag cgccgagcaa cagcagaact acctcccggg tgtgcccgaa ggctccgacc
     8341 tccgcttaac ccctgtctcc gccccggaga accactacgt ccgcatccac gctgacaatc
     8401 gcctagacga gaaaggcacg ctccgcggac aattcacgat caccgccgag ggacaatccg
     8461 acagcaatat ccgccgcatc ttcaccaccg gatggcaatc cgactggcag aacacgatgg
     8521 agaagcaatt gctggccgta tcccccaacg ccaaactgct tcgtgtagat tacggtaagg
     8581 acccgaagaa ctatcaagcg acccctatca agatcacgtt ctcttatgag atccccgatt
     8641 acgcgatcat cgccggagac cagatgctta tcaagccgct agtcatgaat aacctctaca
     8701 atcaagtgaa gagtttcttg cgtatcgata cgaaaccgga aaaccgtacg tatggattca
     8761 aggacgcttg ctcccgtttg gtagaacaag atgagaccat ccgcctcccc aagggctata
     8821 ccctcttgaa cgccccgaag aatgacgccc tgcaaagcaa tgcggccgat ttcgaaggtt
     8881 ccctttcgca ggaaggcgac cagaacatcg tcttgcgcca gaagctagcg ttgaagaagc
     8941 gtgtctacga cgcagccgac tggaaaggtt tccgttccgc cgtgaacgct tataagagtt
     9001 atggtgacta tttgattatc aagaaataaa aacagaaaga caatgattaa gataacctat
     9061 aaactgattc ttctcctaag cttgttagcg atgacggtta cttcattcgc cgcatcggag
     9121 gctgagtacg ggaaggtatc gaaagcttgg accttgcatg ccgacggtag ccaagaatat
     9181 cgatcctcca tggaattgac gctcttcacg cacactgcga tgaatagcac gtatggcgag
     9241 agttttatcg tctacaatcc cgatttccaa acactgaaga ttcactcatc ctatacgagg
     9301 cagaaagacg gtacgatcgt aaagacaccc gataacgcct tcgtggaggt cctgccccgt
     9361 ttcgccgccg acgctccggc ctataaccaa cttaaggaaa tggtcgtggt acacactggc
     9421 ctagaacttg gggccacgat ttacttggat tactctatta tcacaaaacc gggctactat
     9481 ccggctctgg atatcaacga gcgactacaa gagacttctc ccgtgaagga atgcaaggtc
     9541 tctatctccg taccggaaaa cagtccgctc gcttggcaat tattgggtag cgacgcaaaa
     9601 gcatcccaag ccacccgaaa cggcgctaaa gaggtaagct ggactttacg taacctgccg
     9661 gcctcttccc gggaagcttt ccttcctcaa aaccaagacg ggattccccg cctaatcgct
     9721 tcaagctata gttccaacaa ggaggcatta tctacgctaa ataagcgttt taacacttcc
     9781 ggaaactacg agagcaaaac gttcggctct tatatcacgg aaaaggctac tagcgacgaa
     9841 gagaaggtga acgccatccg gaaccatgta gtcaacaaca tgggatattg cgccatcccc
     9901 ctcgcttgta cgggttattc catccgcaat gtcgatacgg cccttcgcag cgcttacggt
     9961 acattagccg agaagaccca gcttctgaac gtcttattga acgccgtcgg cattcaatcg
    10021 gaagtagtcg ccgtatatcc cgggaatctg gatatagacg cttgtggatt aagcacgatc
    10081 aagaatcttg ccgtaaaagc gactgtcgca gggaaagaca aatacctttc ggcggtcagc
    10141 cttgcccctt ccgcattaac agcccggggt gagttagatc aggtactcac gttgaacggc
    10201 acgtcgatcg ccctacaggc agagcctacg atcatcaagg agaataaagc cgtcagcgtc
    10261 tccaagaacg aagcacaaaa cggattcgcc atctgtaccc ttccggctat aacaggcggt
    10321 atcgatagct ggggcatgag cgccttgaac agcaagcgtt ccaacttatt cgaactcccc
    10381 tccctgatca aggaagaggt aacctacacg gttactcccg ccgagggcat gaagttgcag
    10441 acctccacaa aagagcaagt gataagcaag ccttttggta aagtaacccg cacgatcaca
    10501 ccaagaggaa atacgatcga ggtagtacgt acgatcgagt tggacaagca acagttcact
    10561 cccgccgaat atagcgacgt gcgtagcttg atccatgaat ggacaaatcc ggataaccgg
    10621 gtattgctat tctccctata agatctaaaa cacggagcac acggaacaaa tccgtgccct
    10681 ccgtgtgctt cgtgtctaat tattccctac atttacgaac gttaacatta aatcatatcg
    10741 catatgaaaa cactaaagta tttactcgtc accatgctcc ttctaggcgt ttgcagccta
    10801 acgcatagcc aaaagctatc cctaggaatc ttccccgagc cccaagaggt gactatttcc
    10861 agccagacat acgcaccgtc ccatggatat accctacggg gtataaataa tccagacgca
    10921 gatgcggtca aactacttaa agaagcgcta cctttcgcta aatccggcaa atctctccct
    10981 ttagagatca agaagctaaa agacaaaact ccggaaatgc aacgctcggg tgcctatacg
    11041 ctgacgatca ctaaaaaagg catcacaatc gggatcgtag acgataattc cttgttttac
    11101 gccgcccaaa ccctcaagca attagctaaa tatgacgagg gtaagaagat cctgcctctc
    11161 tgctcgatca aggactatcc ggacgtatta ttccggggta ccgtggaagg attctacgga
    11221 cagccttgga gccacgccga ccgtatcgag caaatccact tttacggaag gatcaagctg
    11281 aacacctata tctatggtcc gaaagatgat ccttatcaca gctcccccaa ctggcgcaaa
    11341 ccttatcccg ccgaggaagc cgagcatatc aaggaactag cgaaagaggc cgcccataac
    11401 aaagtaaact tcgtttgggc catccacccg ggacaagaca ttcaatggaa cctgacagat
    11461 agcatgaaca tcctttccaa attcgagaaa atgtacgatt tgggcgtacg ctctttcgcc
    11521 gttttcttcg atgatatatc cggagaggga gcccgcccgg aaaagcaagc cggcttattg
    11581 aattacatcc ataaggaatt tatcagcaaa aaaaatgacg tgcaacccct gatcatgtgc
    11641 cctaccgaat acaaccgttc atgggctaag accgactatc tggatatcct cggtacccaa
    11701 ctcgatcccg ccatccaaat catgtggacc ggagaccgtg tcgtagccga tatcacgaaa
    11761 gaaggcgtcg agtgggtaaa cagccgtatc cgtcgtcccg cctatatctg gtggaacttc
    11821 cccgtaagcg attattgcca agatcatttg ttgatgggac ccgcctacgg cctagatacg
    11881 caagccgccg gcaccatgac cggattcgta tccaatccta tggaatacgc cgaggcttcc
    11941 aaggtcgcta tattcggcgt tggaatgtac acttggaata tcgagaatta cgatcctaca
    12001 caggcatgga aagacgcttg cgactttatt atgccggagg cctccatggc tttccgcatt
    12061 ttctgcgagc ataactgcga tccgggtccg aacggacatc aataccgccg ggaagagtcc
    12121 gctaactatg tggctcctat ccagacattc ctcgccggat ataagaaaaa cacgttcccg
    12181 gagcaaagcg cgaatcttct gggaacctta ttcgctcaaa tcacggcttc cccgagcatg
    12241 atctacagcc aaagtcccaa taaacgcctg atcgaacaaa tcaatccatg gcttatccaa
    12301 ttcgagttct taggaaaagc cggtacaagt gctctgcata tggcgcacgc ttggtatgag
    12361 aaagaccggt cttatacatg gcaacgctat ctggaaacat ctgccttgct agatagcatg
    12421 aaactgataa accgcacgct gaaccagaag gcacagccta aaggcgtgaa agtcggatcg
    12481 aaagtcttac accctttcat cgtagactta taccgccaga caggtcgtaa cctgctatct
    12541 acggacggta tcgccccaga cgaggtgaag gttagcatcc cttccatatt cacgaatatc
    12601 gaccaattga agagccagcc ttgtgccgag ggtgataata cggtcggtta cgtcccccta
    12661 ttcgaggtag tcaaggtaca gcctaatcaa tatctgggta tcggctggga aatccagaaa
    12721 gaagcggaat ccttctgttt caatcttccg aaaagcaacc aacccggacg ggtattcgag
    12781 tggtctgctg acggaaagag ctggagccta atcccccatg tctccacgga aaacgctaag
    12841 gatacgatcc gcaccatcga tccgaaagcc cgttatatcc gtatgcgaaa taactcggat
    12901 cagcaaatgg agctatatgt actaggattc acggcgacaa cccaacaaga cccgcaaatc
    12961 aatgaggctc ttatgatgta cgatatgaac ctagatacgt ataagaactt gaatccggga
    13021 gaaataattc acataaaatg cgatgatatc aacgccgtat ctttcttcct ctccggaagt
    13081 aacgagaatt tggtatctat caccggcctc tctcaagatg gtgagaaaaa catcgtctat
    13141 caagggaacg taggctacat caaattaaat aaaaccatgt tcgaggattt ctcttcttta
    13201 gaaatcacca caataggaaa agaacctata catatccacc agatcgttag ggaataataa
    13261 ataaaaaagt accgatttct ttctctctta gatggaagaa ggagatcggt acttttttat
    13321 ttattcgtac aattcgggag tatcttattt ttggcacatc tccgcttgta tattcctttt
    13381 aaaagttata cctttaggat gcgaaaaata gaaaccgaaa aaacaatgcg aaaagaagaa
    13441 agatataaag gcgtattaaa ctggtttaag gaaaacgtac cggtagccga gactgaacta
    13501 cattatgaca atccatatca attattgata gcggtaatcc tatccgcaca atgcacggat
    13561 aagcgagtga atatgatcac tcctgccctg ttcgaggctt tccctactcc cgaggtaatg
    13621 gcagctagca caccagaagt cgtctttgaa tacatacgaa gcgtatctta cccgaacaat
    13681 aaggctaaac atctggtagg tatggcgaag atgctgatag aggacttcaa aggcgtggtt
    13741 ccctccgata ttgacgagct acagaaactt ccgggtgtag gaagaaaaac tgcgaacgta
    13801 atcgcatccg ttgtatatga caagccagcc atggcggtag atacgcacgt attccgtgtc
    13861 tccaaccgga tcggcttgac caataatagc aagacaccct tggaaacgga aaaggagcta
    13921 gtgaagaaca ttccggaaga actgatcccc atcgcccatc actggttgat attacatgga
    13981 agatatgttt gcctcgcccg gaaaccgaaa tgtgaggaat gcggattaaa gccttggtgt
    14041 aaatacttcc taaagcaaac atgaggatcg gcaagaaaga cataatggca ttcattgtct
    14101 tgtttttggc tacgatcgtt tgcgtgaggt acttttacaa gaatatgagc gacgagcaat
    14161 ttgtcgccac ggtagatccc tatagcttgg tcatcccgac ccccacagca atttttgcta
    14221 tcaatcgtcc tcctgtattc gagaagatga tattacccat ggagaatata cgcaaggcgt
    14281 tctcggatca tacgcccgct attttcctct ctttaataca gcaaaatccg gatttatcgt
    14341 ctttcttgat cgcttattat ccacaagggg atatcttgta cgcccctatg gatagtcata
    14401 cggcagagcg tattttcaag caactggacg cttctttcac ttttcctgcc cagcaacgag
    14461 aggaagcttc cgttcctgtt agatactatc cggatgttga taagcatttt ctaggttgtt
    14521 actaccacga gggtatattc gtggtgtcct acaatcgaaa attattggta gagacagcga
    14581 aaaagcaaca gatgtacccc gcacagatca ttccggaatt agctgatcta attagtaaaa
    14641 aaggcaagag tggcgcgatg aatctcttca tccaaagcgc ctctttagat ttacaggttc
    14701 aaatgaatga ctcgacggag tggaaaatga aaaatcaatg gttggcaatg gatttattct
    14761 ataatgaagg aagcctttgc tgcttcaatg agcagcccta tgaagaaacc ttggagaact
    14821 tttaccccaa cttatgcgat acgataacta ctcggatcaa tcggctattc ccacaaataa
    14881 aaaccacggc acaagtcagt catgacgagg ccgtggtcta ttttacggtt tgcgggaatt
    14941 aagcttcagt cccttctttc ttgaatgctg acacaaacat a
//
