LOCUS       gnlProkkaJCIMKLNM_499   6564 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JCIMKLNM_499.
ACCESSION   gnlProkkaJCIMKLNM_499
VERSION     gnlProkkaJCIMKLNM_499
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 12:33:39
            Original ID  :: gnl|Prokka|JCIMKLNM_499
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6564
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6564
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1200:6563]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1201..6563
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..6564
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..6564
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             159..683
                     /locus_tag="ctg499_1"
                     /transl_table=11
                     /translation="MSEQQKYWFAARTRDKQEFAIRDSLEKLKTELDLNYYLPTQFVIR
                     QLKYRRKRVEVPVIKNLIFIQATKQDACDISNKYNIQLFYMKDLLTRAMLIVPDKQMQD
                     FIFVMDLDPNGVSFDNDHLSVGSRVQVVKGDFCGVEGELASEANKTYVVIRIAGVLSAS
                     VKVPKSYLRVI"
     CDS             687..1169
                     /locus_tag="ctg499_2"
                     /transl_table=11
                     /translation="MNTLTSQIEQLQSLAHELLYLGVDGAPIYTDHFRQLNKEVLEQSD
                     ALYPQRGATPEEEANICLALLMGYNATIYNQGDKEEKKQVVLNRCWDVLDQLPATLLKC
                     QLLTYCYGEVFEEELAKEAHTIIESWKDKELSEDKKEAINNLRNLEENPYPYSEME"
     CDS             1201..2094
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 268.8; E-value:
                     9.4e-82)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg499_3"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.3e-14, bitscore:
                     41.5, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGIVLAGGSGTRLYPITKGVSKQLLPIFDKPMIYYPISVLMLAG
                     IREILIISTPYDLPGFQRLLGDGSDFGVHFEYAEQPSPDGLAQAFIIGEKFIGSDSVCL
                     VLGDNIFYGQGFTRMLREAVHSAESENKATVFGYWVSDPERYGVAEFDQEGNVLSIEEK
                     PQIPKSHYAVVGLYFYPNKVVEIAQNIKPSPRGELEITTVNQQFLLDQELKVKLLGRGF
                     AWLDTGTHDSLSEASTFIEVIEKRQGLKVACLEGIAFRQGWISSEKMKVLAQPMLKSQY
                     GQYMLKMIDITTSNEE"
     CDS             2267..3571
                     /locus_tag="ctg499_4"
                     /transl_table=11
                     /translation="MIYMFTRMIRSLLSRSPLYVLALYASVLIFLVYTCAYAYRKPFTA
                     AIYEGEILWGFDVKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVAL
                     LGFALLPVPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKT
                     LGQFVMDNLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKD
                     RRKFLRQFFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFV
                     LVLMFLIVFVRNNRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMG
                     YIPFTYLIERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRT
                     ALFSGFVSVLSIFAIYCYFKQQLNSLILIPSEIHD"
     CDS             3585..4283
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg499_5"
                     /sec_met_domain="NTP_transf_3 (E-value: 8e-15, bitscore:
                     44.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             4303..5604
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg499_6"
                     /sec_met_domain="CTP_transf_like (E-value: 1.8e-13,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 6.5e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 3.2e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             5616..6563
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 56.6;
                     E-value: 3.3e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg499_7"
                     /sec_met_domain="TPP_enzyme_N (E-value: 6.8e-23, bitscore:
                     70.0, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.3e-18, bitscore:
                     54.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGVALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYA"
ORIGIN
        1 ctgtttcaaa agacgaacgt tttttgaaac acattcgttc gttctcaact tctaaaaatg
       61 tttcgtaaaa tttgacggtt tgaaagagaa aagatgtctc gttcaaaccg cgaagcgcac
      121 ccgaaataaa ggaaaatttc cccaagtata aatcgtaaat gtctgaacaa cagaagtatt
      181 ggtttgccgc ccgtacccga gataaacaag agtttgctat tcgtgactct cttgaaaaat
      241 tgaagactga acttgatctc aattactatc ttcccactca gtttgtcatc cggcagttga
      301 aatatcgtcg aaaacgggtg gaagtccctg ttattaagaa tcttatcttc atccaagcta
      361 ccaagcaaga tgcttgtgat atttctaata aatacaatat ccagcttttt tatatgaaag
      421 acctgcttac cagggctatg ctgatagttc ctgataaaca gatgcaggac ttcatatttg
      481 tcatggattt agatccgaat ggcgtcagtt tcgataatga ccatttatct gtcggtagca
      541 gggttcaggt agttaaaggt gatttctgtg gtgtcgaggg cgaacttgcc agcgaggcca
      601 acaaaactta tgttgttatt cgtattgccg gtgtattgag cgccagcgtc aaagttccta
      661 aaagttattt acgtgtcatt taagttatga atacactcac ttctcaaatt gaacaattac
      721 agagtctcgc gcacgagtta ctctatttag gtgtggatgg tgctcctatt tataccgatc
      781 atttccgtca gttgaacaaa gaagttttag aacaatccga tgcgttgtat cctcagcgtg
      841 gtgctactcc cgaagaggaa gcaaacatct gtctggcatt attgatgggt tacaatgcaa
      901 ccatctataa tcagggtgac aaggaggaga aaaagcaagt tgttctcaat cgttgttggg
      961 acgtgcttga ccaacttcct gctactttat tgaaatgcca gttgttgact tattgttatg
     1021 gtgaggtgtt tgaagaggag ttggcgaaag aggcgcatac tatcatagaa agttggaaag
     1081 ataaagaact ttctgaagat aaaaaagagg caatcaataa tttgagaaat ttggaagaga
     1141 atccatatcc ttatagtgaa atggaataga ataattctat tttttttatt tatttgactt
     1201 atgaaaggca ttgttttagc tgggggatct ggtacacgtt tatatcctat tactaaagga
     1261 gtaagcaaac aacttcttcc aattttcgat aaacccatga tttattaccc catctctgta
     1321 cttatgttag ctgggatacg agaaatacta attatctcta ctccttatga cttaccgggt
     1381 tttcaacgtt tactgggcga tggttctgat tttggagtac actttgagta tgcagagcag
     1441 ccttctcctg atggtttagc acaagcattt attattggag agaagtttat tggcagtgat
     1501 tctgtttgtt tagttttagg agataatatt ttttatgggc aaggttttac gcgcatgttg
     1561 cgtgaagcgg ttcattctgc tgaatcagaa aacaaagcaa ccgtttttgg ttattgggta
     1621 agtgatcctg aacgttatgg cgttgcagag tttgatcagg agggaaatgt actcagtatt
     1681 gaagaaaagc ctcaaatacc taaatcacat tatgctgttg taggacttta tttctatcct
     1741 aataaagtgg tggagatagc ccaaaatatc aaaccttctc ctcgtggaga attagaaata
     1801 actactgtta atcaacagtt tttgttagac caggaactga aggtcaaatt attagggcgt
     1861 ggctttgctt ggttggatac aggtacgcat gactcgctgt ccgaagcgag cacttttatc
     1921 gaagtgatag aaaaacgtca agggctgaaa gttgcttgtt tagagggaat tgcttttagg
     1981 caaggttgga tttcttccga gaagatgaaa gtattggcac aaccgatgtt aaagagccaa
     2041 tatgggcaat atatgttgaa gatgattgat ataacgacct ctaatgagga atagatgcct
     2101 gaattagata gtaaaaaaat taataatact gacttcttca aatatagcta atccttataa
     2161 ttctttattt atggataaat agatttctga gtgtttttta tttagcgaat agaatatttt
     2221 tacattcagc cctgttaaat cgtaatttga attgtcgtta cattggatga tttatatgtt
     2281 tacacgaatg attcgttccc ttctttcccg ttctcccctt tacgttttgg ctctctatgc
     2341 cagcgttttg atttttctgg tttatacctg tgcctatgcc taccgtaagc catttaccgc
     2401 agccatttat gaaggtgaga ttctttgggg attcgatgtc aaaatcctgt atgtcctttc
     2461 tgagattatc ggctacgccc tttccaagtt catcggtgtt cgtattctgc cttcgatgaa
     2521 agcgggtcac cgcatctatt atataatagg gctgcttact ttttccgaag ttgctttgtt
     2581 gggttttgca cttcttccgg ttcctttgaa ggtgtgttct atttttctga gcggtcttcc
     2641 cctgggtatg atctggggtg tcatttttag ctatatcgaa gggcgtcgta tcagtgaaat
     2701 attgaatgtc gggctgagcg ttgctttgat agtctcttcc ggtttggtga aaaccttggg
     2761 acaattcgtg atggataatt tgcatgtgtc ggaatattgg atgcctttta ccaccggttt
     2821 gttggtatac cctgttatgt tgctttgcag ctggcttctc aatcagattc cggctcccaa
     2881 tgagcaagat atcatacagc gtaccaaacg tgctccgatg gatggtaaag acaggcgtaa
     2941 gtttttgagg caattctttt ggggaatctg catgctggtt gttttttacg gtgccctgac
     3001 cgtgttccgt gagcttcgtg actctttcgc tgccgatatt tggaaggagc tccatattga
     3061 gggtgcaatg atctttactc agacagaggt tcccattgcc gtttttgtcc ttgtactgat
     3121 gttcctgatt gtgtttgtcc gtaataaccg tttggctctt aatatcattt attgcatcgc
     3181 cgtcacaggc ggttccttaa tggttttttc cacattgctt tatgtgtacg gttttctttc
     3241 tcctatttgg tggatgattc tttccggtct tggtctgtat atggggtata ttcctttcac
     3301 ttacctgata gaacgtctga ttgcttccct tagggtggtt agtacggctg tttttattat
     3361 ttatttggcc gattctttcg gctatctggg cactacgggt gtgtttatgg tcaagaactt
     3421 cgtttctgct gacatttcct ggactgtcat gctgatgcgt actgcccttt tttcaggatt
     3481 tgtttctgtc ctgtcgattt ttgcgattta ctgttatttc aagcaacaat tgaattcact
     3541 tatattaatt ccctctgaaa ttcatgatta aaacagcaat gatcatggct gccggtcttg
     3601 gtacccgctt cgggcattat accgagcttg tgcccaaagg ttttgtggag gttggtggaa
     3661 aacctatgat tatccgctct atcgaaaccc ttctttcctg cggtatcgaa cgtatcatcc
     3721 tcggtacggg ttataaaaaa gaggcttatg aagcccttca ggcggatttc ccccagatag
     3781 agacctgttt cagtccccgt tatgccgata ctaacagtat gtacactctg tacaatacgc
     3841 gtgacgtgat aggcgatgac agtttcctgc tgcttgaatc ggaccttgtt tttgaacgca
     3901 aggccatcct ctcgctgctg gatgacgaat tcccggatgt gatgcttgtc tcttctctga
     3961 ccaagttcca ggatcagtat tatgtggaat atgaccgcaa tcacatcctt acttcctgtt
     4021 cggtagacaa ggacgccctt gaggcaaaag gcgagctggt cggtatccat aaactttcca
     4081 atactttcta ccgccggatg tgcgccgatt atgcgaccat cctggagtcc cagcctaaac
     4141 tgggttacga gtacgaactg ctccgcatgt cccgttccgt ttcccctgtc cgtgtgctca
     4201 gggtggaggg tcttaaatgg tatgaaatcg atgatgaagc cgacctttcg tatgctgagg
     4261 agcacatcat ccgctattgt taaaaacaaa tcttttccat atatgaacaa gaaaatctat
     4321 gtcggtatga ccgcggatat catgcatccg gggttgatcc atattattca tgaagctact
     4381 aaatacggtg atgtcatcgt cggtctgctg acggacaagg ccatcgccga acacaaacgc
     4441 ctcccttacc ttacctatga tcagcgtaag gaggttgttg agaatatcaa aggggtttgc
     4501 gaagttgttc cccaggagga ttggagctat gttccgaatc tggaaaaact caagcctgac
     4561 tatatcatcc acggcgacga ctggaaaacg ggcccgttgc gcgaggtgcg cgaacaggtt
     4621 tttgaggtga tgaacgccca ggggggcaag gtaatcgaga tcccttatac ccggggcatc
     4681 aactcctcct cgctcgacaa ggatatcaag gcgatcggga ctacccctga cgtacgtctt
     4741 aagtccctcc gccgcctgat ccaggccaag cctgtggtcc gtatcctgga ggctcacgac
     4801 ggtctttgcg gtctgattat tgagaatctt gaagtccaga agggtgataa gcgcgaggtc
     4861 tttgacggca tgtggtccag cagcctgacc gactccacga gtaagggtaa accggatatt
     4921 gaagcggtcg accttaccac ccgtttacag gacctgaaca atatccttga atgtaccacc
     4981 aagcctatta tttttgatgg agacacgggt ggcaagatcg aacactttgt ctttactgtg
     5041 cgcactctgg aacgtcacgg catttctgcg gtaatcattg aagataaagt cggtctgaag
     5101 aagaattccc tttttggtac tgacgctatc cagactcagg atagtatcga gggtttctgc
     5161 gataagatca gagcagggaa gaatgcccag gtcaccggtg actttatgat cattgcgcgt
     5221 atcgaaagcc ttattgccgg caaacccatg agtgacgccc tcgaacgtgc ttatgcctat
     5281 gtacaggcgg gtgcggacgg catcatgatc cacagcaagg ataagtcggg tgaggatatc
     5341 cgtgagttct gccggacttt ccgcaaagaa tacgcccatg ttcccattgt cgttgttccg
     5401 acgacttatg accatgtgca cgaatccgaa cttcacgaat ggggtgccaa cgtagtcatt
     5461 tacgccaacc accttctccg tgccgcttat ccggccatga tgaacgtggc caggaccatc
     5521 cttgaaaacg agcgcgccga ggaggtccgt ccgctttgta tgcctatcaa ggaaattctg
     5581 gaattgattc cgggcactaa ataaatcaga cggttatggt aagtgtagaa aagatatacg
     5641 atacgttcct tcatcacggt gttgacttct ttacgggtgt tcccgattcc cttttgaaga
     5701 atatttgcgc ctatatcacg gatcatgctc cgcgtggtaa gcatatcatc gccgccaatg
     5761 aaggttccgc ggtgggcatt gcctgcggtt attatatggc ttctggcaac gttcctttgg
     5821 tttatatgca gaactccggt ctgggaaata cggtgaatcc cctcttgtcc cttgcggacg
     5881 agaaagtgta cagccttcct cttcttttgc tggtcggttg gcgcggtgag cccggcacga
     5941 aagacgaacc ccagcataag aagcagggcg aggttaccct tgatcttctg aaagccatga
     6001 aaattccttt tataattctg gatacggatg aagacgaggc cctggcgcag atccatgacg
     6061 cggttgtctc ggcgaagagc cggtcgattc cccatgcgat tgtcatccgt aaagatacgt
     6121 ttggaaaata taaattgcgt caggaagcct tgaatgacta tcctctgtcc cgcgaggagg
     6181 cgctaatgca ggttgtcgac cacctcgggg acaatgacat tgtggtttcc accacgggca
     6241 agctctcccg tgagcttttc gagtaccgtg aatcgaaagg gcaggggcac gcaagggatt
     6301 tcctaactgt gggttccatg ggtcacagtt cttccattgc cctgggggtt gcccttgaga
     6361 agcctgaccg ccgtgtctac tgtcttgacg gtgacggtgc ctttatcatg cacatgggtg
     6421 ctgtcagcaa tatcggcgat ctttctccca agaattacag gcacgtcctt ttcaacaacg
     6481 gtgcccatga atctgtcggc ggtcaaccca cgctcggttt ccgtctggat atcgcctcga
     6541 tagcgaaagg ttgcggttat gcgc
//
