LOCUS       c00475_gnlPro..         7188 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JCIMKLNM_475.
ACCESSION   c00475_gnlPro..
VERSION     c00475_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 11:17:38
            Original ID  :: gnl|Prokka|JCIMKLNM_475
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7188
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7188
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[52:3011]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      53..3011
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7188
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7188
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             53..2281
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg475_1"
                     /sec_met_domain="Gly_radical (E-value: 2.1e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2286..3011
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg475_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.9e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEAGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFQTVQVN"
     CDS             complement(3243..4502)
                     /locus_tag="ctg475_3"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVLTTAVSLYFLNSPVADEIRYATAPSLAVNPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLVEKRPEAVTEEKRSEPQQEAIAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSENFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVVDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(4557..5105)
                     /locus_tag="ctg475_4"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
     CDS             5293..6045
                     /locus_tag="ctg475_5"
                     /transl_table=11
                     /translation="MKKFNLITFAVILMLLPILQSCLDDANNDEWVTCPPGGILAIGTM
                     KIPNADTPRDFFIALDNGDNVLPADTADIRNRKYTVAEGQRVFVGYLQMGEEKPGYENG
                     KIFTIEDILTKEIIPLTEATADSIGDDRINVTAHALTKDYLTIEYQYLGSMNENKKHML
                     NLVQNEITGPIKDDGYIYLEFRHNAFNDSPNQLGSSLVSFKLDSIAEQLATAKGIKLRV
                     NTIYDNIQYVTIDINEDKNLKIKSFHSQ"
     CDS             complement(6150..7148)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     -3.5; E-value: 38)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg475_6"
                     /sec_met_domain="LysR_substrate (E-value: 1.1e-47,
                     bitscore: 150.8, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 4.5e-19, bitscore: 57.0,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVKLKISIFALIDSISLINQRTMTIQQLEYILAVDQFRHFAKA
                     AEYCRVTQPTLSAMIQKLEDELGVKLFDRSMTPVCPTAIGKKVLEQARKILSEVICVKE
                     IISEEQHSLSGTFRLAVLPTIAPYLLPRFFPQLMEKYPDLDIRVMEMKTPDIRKALLTG
                     EADAAIIASMLDDAALTEETLFYEQFLGYVSKKEPLFKHDVIRTSDVTGERLWLLDEGH
                     CFRDQLVRFCQMEAVKISQMAYRLGSMETFMHMVESGKGITFIPELAVMQLSEEQKELV
                     RPFAIPRPTRQIVLVTRKDFIRTSLLQVLKEEIQAAVPKEMLTLQAVQCLV"
ORIGIN
        1 gttgtaaatt tgcgcgctct aaaggataac taaccactaa ctgattaatt ttatggaact
       61 gaataagacc tttaaagatg gactttggag caaagaaatc aatgtaaggg atttcgttag
      121 taacaacatc actccttatg aaggggatgc ttctttcctg caaggaccta ccgaacgcac
      181 caaagctgtc tggaatcact gtctcaaagc tctcgaagaa gagagaaaca ataatggtat
      241 tcgtgcactc gactacacaa ctgtatctac tataacctct caccctgccg gctacatcga
      301 taaagaaaac gaactgatcg tcggactaca gaccgatcag gtgttaaaac gtgccatcaa
      361 accgtttggc ggaatcaatg tggttatgaa agcttgccgt gaaaacggag tggaagtaga
      421 tgacagagtt aaggatatct tcactcacta tcgcaagact cataatgatg gggtattcga
      481 tgtgtatacc gaagaaattc gttcgttccg ttcattggga ttccttaccg gacttcccga
      541 caactatgca cgcggacgta tcatcggtga ttatcgccgc ctggctctct atggtcttga
      601 ccgtctgata gaagctaaaa aagaagatct gcataatctg acaggtccta tgactgaagc
      661 ccgcattcgt cttcgcgaag aagtagccga gcaaatcaag gccctgaaag agattaaagt
      721 attgggcgaa tattatggtt tggatctgag ccgaccggca tataccgcac aggaagctgt
      781 acaatgggtg tatatggctt atctggctgc cgtaaaagag caggatggtg ctgccatgtc
      841 actgggtaat gtatcttcat tcctcgacat ctatcttgaa tatgaattaa gccagggtac
      901 gataaccgaa acattcgctc aggaactgat cgaccaattc gttatcaaac tccgtatggt
      961 acgccacttg cgtatgcagt cgtacaacga tatttttgcg ggtgatccca catgggtgac
     1021 cgaatctatc ggtggacgtt tcaatgatgg acgtaccaaa gttactaaaa cttctttccg
     1081 cttcttgcag acactttata acttaggccc gtcaccggaa cctaacatga ctgtactatg
     1141 gagccctgag ttacccgaag gtttcaaagc attctgtgca caagtatcta tcgacacttc
     1201 ttccgtacaa tacgaaaacg acgacctgat gcgtgaagtc cgtcaatcgg atgactacgg
     1261 aatcgcttgt tgtgtttcat atcaggaaat cggaaagcag attcagttct tcggcgcacg
     1321 tgccaacctt gccaaagccc tgctactggc tatcaacgga ggacgttgcg aaaatacagg
     1381 tacagttatg gtaaaagata tccccgtatt gaccggtgaa acactgaagt tcgaagaagt
     1441 aatggccaac tataagaaag tattgataca aattgcacgt gtatacaacg aagcaatgaa
     1501 tatcatacat tacatgcacg ataagtatta ctacgaaaag gcacagatgg ctttcgtgga
     1561 tactgatccc cgcattaacc tcgcttatgg tgtagccgga ctttcaatcg ccatcgattc
     1621 actgtccgcc atcaaatatg cacatgtgaa agcccgtcgc aacgacatcg gactgacgga
     1681 gggattcgat atcgaaggtg ctttcccttg tttcggtaat gacgatgacc gtgtagacca
     1741 cctgggtgtg gacctggtat atttctttag cgaagaatta aagaaactcc ccgtatataa
     1801 gaatgcccgt cctacccttt cgctgctgac tatcacttcg aacgtaatgt atggcaagaa
     1861 gaccggtgcc actcctgacg gacgcgccaa aggcattgcc tttgctcccg gtgccaaccc
     1921 catgcacgga cgtgataaaa atggtgcaat cgcctcactg agttctgtag ccaagttgcg
     1981 ttatcgcgac tcacaggatg gtatcagcaa tacattctcg atcgttccca aatcattggg
     2041 agcaacacaa gaggaccgta tcgacaatct ggtgaccatg atggacggat attttacaaa
     2101 aggcgcacac cacctgaacg taaacgtgct gaaccgggaa atgttgcagg atgcgatgga
     2161 acatcccgaa aagtatccgc aactgactat ccgtgtctca ggatatgccg ttaatttcgt
     2221 gaaattgagc cgcgaacatc agttggaagt aatcagccgc agtttccacg aaagaatgta
     2281 attgaatgat aaacgtacac tcatacgagt caatgggaac attcgacggg ccgggtctcc
     2341 ggctcgtcgt tttccttcaa gggtgtaact tccgttgcct ctattgtgcc aatccggaca
     2401 caattgaagc caaaggaggg acagcaaccg accctgagga aatagttcgc atggccgtca
     2461 gtcagaaagc tttcttcggt aaaaagggag gtatcacctt ttccggcgga gagcctactt
     2521 ttcaagcaaa atcgcttatc ccacttttta aaagactaaa ggaagcagga atccatattt
     2581 gccttgatac caatggcggg ctatggaaca atgatgtaga ggaactattg gaattgactg
     2641 atctggtttt attagatatc aaagaattta atccggaaca tcatcagtct ttaaccggaa
     2701 gaagcaacga gcagaccctg aaaaccgcag cctggcttga aaccaatcat aaaccattct
     2761 ggttgcgata cgtattagtg cccggctaca gtgacttcga agacgatatc cggcagctgg
     2821 gagagcatct tggaacgtac cagatgattc agcgtgtaga aatattgcct taccacacct
     2881 tgggtgttca caaatatgaa gcaatgaaca aagaatatat gctgaaagga gtgaaagaga
     2941 atactccaga gcagatagaa aaggctgaga aactattcag gcaatatttc cagaccgtac
     3001 aagtaaattg acttatataa gtgtcgaaat tcgtattcac cacagattac acgaatttac
     3061 acaaataaag atcattggca atcaatagat taagttcatt ctgcggtgaa ttatgacgac
     3121 tgcacttatt tataattttg taagtcacga tataaaagtc tccctgttct tgccttaatt
     3181 cattacaata caaggcaaga acagggagat aatgtaaccg gcatattttt tgccatccta
     3241 ctttaataag tgaatcgcaa tcctgcctgc aagttaaagt tacaaggcct ttcttttcgg
     3301 atggtttgta ctttggagcc atcatcaaag aaatacgaaa ctcccggttc cacatatagg
     3361 ccgacatgat ctgtcgcatt gaattgcgct cccacggcac cggctacaga aaattgcaaa
     3421 ggcttcacat tcaccttatc atccgccaac tttccataaa cacacttttc aaccattcct
     3481 ccggcagata catagagtgt aaaatatttc ttttccagaa aattccagtt tccacgtaca
     3541 ggaataccta tataatgcaa tttctgactc tgcatcttgg tatccccctg ccgtttgatg
     3601 tcagaagaca acaacgtata agtcaatccc gtctctacag agaaaccttt gggaagagac
     3661 ttacgtacag acagtccgaa gctaaccggc tgatgatgtt cataatccac cacggcagtt
     3721 gtattggact tcaggtaagg gactccttct tcaaatataa cttcctgatc ggcaggaata
     3781 ctcacagctc cattcatgat cgtcatcaag tcgaccctgt tagtcatcgg acggggcgca
     3841 aaattttcgg aaccatttgt cggaagtcca cctccattac cgatacccac tcccatcgac
     3901 catttaccac cacgtttagc agaagagtct ccgataggca actgatactt gtctttaccg
     3961 gacggtcgcc gcgcttcttt cctctttgga ggctgcgccg tcgcactttc ttttttctcg
     4021 attgcagcaa ttgcttcctg ttgcggctca gatctctttt cctccgtcac tgcctccggt
     4081 cgcttctcta ccagggagga tacctccgga acaatcaccg gatcagtatt ctttgccaaa
     4141 tagcccgaaa cagggttaat actcgtcgtt cctaccggct taactggttc ggatacagct
     4201 acctgtacat ccggcaaggc aggttccggc aatacatccg ggttgacagc cagtgaagga
     4261 gccgttgcat aacggatttc atcagctacc ggactattca gaaaatacaa actgacagca
     4321 gtcgttagca ccaataccac agctgccgca gcaaccgccc accggcgata cggatatatt
     4381 cgtttttcgg ttacaggcat cagttccctt tccagtcgtt cccaaccgga agcaggcatc
     4441 ggctccgaat aatcttcgag cttatccttg aatgctttaa tccatttttc gtcttctttc
     4501 atcattctat ttcatttttg aaatccaagg ttcacctcag tatctgtaaa tacatgtcaa
     4561 ccattggtca ccaaccattc tttcactttc tttgccaata cactttttgc gcgaaaaagc
     4621 tgtgaggcag aagatttttc attaatcccc aacatctgtg cgatttcctt gtgcgactta
     4681 tcttcaaagg tgtataaatt gaatacggta cgatatccgg cggggagttc attaataaat
     4741 tgcatcagca ctttctgtgg tattgcttca acagccgaag catccggttc ttcatacgtt
     4801 tcgggaactt catccagtgc cgtggtctgg ttcatcacgt cgttctttct taagaactgt
     4861 aaagccgtat tcaccattac acgctccatc catgctctca gagagccttc accccgccag
     4921 gtaaacttgt cgaaggagtc gaaaatcttc aggaaaccgt cgtgtaccaa gtcttgtgcc
     4981 atgtccctgt ctccggcata acgaagacag acaccaagca tgcgccctgc gtactgctca
     5041 taaagttctt tgcgggcacg attatctccc tgcctgcaac gttccgacag ctctaattca
     5101 ttcattctcc taaacacgtg tttaccttat aaatgcagca aagatagaaa tactgcacag
     5161 agagaacaac tcttttatca cttttgtttc tttaacattt taggatgcaa tatttcatca
     5221 cttcctgcat tttctcaata caataaagaa taaatctcaa tggataaata ggataaaacc
     5281 aacaagaaac ttatgaagaa atttaattta ataacttttg ccgtcatcct gatgttattg
     5341 cccattttac agtcatgtct ggacgatgcc aacaacgacg agtgggtcac ttgtcctccg
     5401 ggtggaatat tggccatcgg cacaatgaag attcccaatg cggacactcc aagggacttt
     5461 ttcattgcgt tggacaatgg agacaatgta cttccggcag atacggccga tatacgcaac
     5521 aggaaataca ctgttgcaga aggtcaaaga gtcttcgttg gctatctgca gatgggagaa
     5581 gagaaacccg gatatgagaa cggtaaaata ttcacgatag aagatatatt gacgaaagag
     5641 atcatcccac tgacggaagc tacagcagac agcattggag acgaccgtat caacgtcaca
     5701 gcgcatgcac ttacgaaaga ttatctgacc atcgaatatc aatatctggg cagcatgaat
     5761 gaaaataaaa aacacatgct gaacttggta caaaacgaaa taacaggtcc cataaaagat
     5821 gacggatata tctatctgga gttccggcat aatgccttta atgactcccc caatcaattg
     5881 ggttcgagcc ttgtttcttt caagctggat agtatagccg agcaattggc gacagccaaa
     5941 ggaataaaac tccgtgtgaa caccatttat gacaatatac agtatgtcac aatagatatc
     6001 aacgaagaca aaaacttaaa aataaaatca tttcattctc aataaaacaa caagaaacac
     6061 ctacacaatc aggcaaaacg agtaataatt ctgatacata aaagaaataa ggcggcgatg
     6121 tttgtgaaaa tatcgccgtc gtttttatat tataccagac actgaacggc ctgcagggta
     6181 agcatctcct tgggaaccgc cgcctgaatc tcctctttca aaacctgcag cagactggtg
     6241 cggatgaagt ctttccgggt caccaggaca atctgacggg taggccgcgg gatagcaaac
     6301 ggacggacca gttctttctg ctcttcgctc aattgcatca ctgccagttc gggaataaac
     6361 gtaatcccct taccactctc caccatatgc ataaaggttt ccatactacc cagccgatac
     6421 gccatttggc tgatcttaac cgcttccatc tgacaaaaac gaaccaactg atcgcggaag
     6481 caatgccctt cgtcaagaag ccaaagccgt tcccctgtca catcggacgt acgaatcaca
     6541 tcgtgcttga acaacggttc cttctttgag acatagccca ggaattgctc gtagaataag
     6601 gtctcttccg ttaatgcggc atcatccagc atgctggcaa tgatagcagc atccgcttca
     6661 ccggtcagca gagccttgcg aatgtcgggc gtcttcatct ccatcacccg tatatcaaga
     6721 tccgggtatt tctccatcag ttgcggaaag aaacggggca gaagataagg tgcaatcgtc
     6781 ggtaacactg ccaatcggaa tgtacccgac agggagtgct gttcttcgct gatgatctcc
     6841 ttgacacaga taacttccga aagaatctta cgggcctgtt ccagcacttt cttaccaatg
     6901 gcagtagggc aaaccggtgt catgctacgg tcgaacagtt tcacccccaa ttcgtcttcc
     6961 agcttctgga tcattgcact caaagtaggt tgcgtcaccc ggcaatactc ggcagcttta
     7021 gcaaaatgcc ggaactggtc gacagccaga atatattcca attgttgtat agtcatcgtt
     7081 ctctgattta ttaaagatat agaatctatc aatgcaaaga tagaaatttt cagtttgaca
     7141 cgaatcattt tgtgccgtat ctttgttcca gataaaaaga agaaatat
//
