LOCUS       c00444_gnlPro..         5448 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CKMDKBCG_444.
ACCESSION   c00444_gnlPro..
VERSION     c00444_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 15:26:20
            Original ID  :: gnl|Prokka|CKMDKBCG_444
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5448
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5448
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:4915]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..4915
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..5448
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5448
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             2..1288
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg444_1"
                     /sec_met_domain="HBT (E-value: 8.1e-19, bitscore: 56.4,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SVVHSISYLCNLNQNRVIMKYKLLVLDVDGTLLNDAKEISKRTLA
                     ALLKVQQMGVRIVLASGRPTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFER
                     RINPEMLPYLEKKARKNGFALFTYHDDTIITDSPENEHIQNEARLNDLQIIKEEEFSAA
                     VDFAPCKCMLVSDDEEALLGLEDHWKRRLNGALDVFRSEPYFLEVVPCAIDKANSLGAL
                     LEVLGMKREEVIAVGDGVCDVTMIQLAGLGIAMGHSQDSVKACADYVTASNEEDGVAVA
                     VEKAIISEVRAAEIPLDQLNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGI
                     LHGGATLALAETVAGLGSMILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRS
                     SHVWNVDVFTSTNKLVSSIRVVNSVMKKR"
     CDS             1285..2400
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg444_2"
                     /sec_met_domain="Chorismate_bind (E-value: 6.8e-58,
                     bitscore: 184.6, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGAVRL
                     IYDLKELNGQRGFVIAPFQVSESCPVVLIQPDQWGQPLPVDDDTEEDREIALRLQGQES
                     FLTSSTEEYAACFHTFINALRDNTFDKLVLSRHLTIDKVSGFSPLSIFRAACRRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLGIHSTENGPYPAYAGALSHLKTDFRFSLKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYRFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIKDEQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIATPPLKS"
     CDS             2420..4087
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg444_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.2e-08, bitscore:
                     22.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYAVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVVISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINVPISEPLFQFTVDALPEVRVITRYQGLNVYDRDYNDLVDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKIDQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPDYPRVWENYCKIIPEPDFAYSEMLAVGTLLKALPESCALHLANSSVVRYAQLYSIP
                     PTIEVCCNRGTSGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNINVRSNLRI
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELTEAM
                     KTFTQPETMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             4091..4915
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg444_4"
                     /sec_met_domain="ECH_1 (E-value: 4.6e-83, bitscore: 266.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             5088..5447
                     /locus_tag="ctg444_5"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILKRFCRQVEKEQGMWDKDVLCQYPSILFGLETAIWHFF
                     AGSWALSDTAFSRGEV"
ORIGIN
        1 ttctgtcgtt cactcaattt cctacctttg taatctaaat caaaatcgtg taatcatgaa
       61 gtataaatta ttggttcttg atgtagacgg aacactcctc aatgatgcga aagaaattag
      121 taagcgtaca ttggctgccc tgttgaaggt tcagcaaatg ggagtgcgta ttgtgctggc
      181 atccggcaga ccgacttatg gcctgatgcc gctggccaag atgctcgaac ttggaaacta
      241 tggaggtttt atcctttcct ataatggctg tcagattatc aacgcacaaa acggggaaat
      301 tctgtttgaa cgtcggatta atcccgaaat gttgccgtat ctggaaaaga aagcccgtaa
      361 aaacggtttt gctttattca cttatcatga tgatacgatc ataacggatt ctcctgaaaa
      421 tgaacatatc caaaatgaag cccggttgaa tgacctgcaa ataatcaaag aagaagaatt
      481 ttctgctgcc gttgattttg ctccttgtaa gtgtatgctg gtgagcgatg acgaagaagc
      541 attgcttggt ttggaagatc actggaagag acgactgaat ggggcgttgg acgtattccg
      601 ttcggagccc tattttctgg aagtagttcc ctgcgccatc gacaaagcca attctttggg
      661 tgctttgctg gaggtgctag gtatgaagcg tgaggaagtg attgctgtgg gcgacggtgt
      721 gtgtgacgta acaatgattc aattggcggg gctgggtata gctatgggac attcacaaga
      781 ctcggtgaaa gcctgtgccg attatgtgac tgcttcgaat gaggaagacg gagtggccgt
      841 tgcagtggag aaggctatca tatccgaagt gcgtgcagcc gaaattcccc ttgaccaact
      901 gaatgcgcag gcacgccacg cattaatggg aaacttggga attcaatata catacgccga
      961 cgaagaccgt gtggaagcaa cgatgccggt agaccatcgt acccgtcagc ctttcggcat
     1021 tttgcatggg ggagctactc ttgcactggc agaaacagtt gccggactcg gttcgatgat
     1081 tctttgccag ccggacgaga ttgtagtagg aatgcaggtc agcggaaacc atatatcttc
     1141 tgcacatgaa ggagatacag tgcgtgcggt aggaacagtt gtacataagg gacgttcctc
     1201 ccacgtatgg aatgtagacg tgtttacttc aacaaacaaa ctggtgtctt ccatacgggt
     1261 agtcaacagt gtgatgaaaa agagatgacc gacgaagaaa taagtaactt gacaactatt
     1321 gatgcattta ttcagcggaa gcagccgttt gctgtttacc gtattcccgg agagaaggtt
     1381 ccccgtctgt tgacgcaggc ggaaggagcg gtccgtttga tatacgattt gaaagaactg
     1441 aatggacaga gaggatttgt aattgctccg tttcaggtga gtgagtcatg tccggttgtg
     1501 ttgattcaac ccgatcagtg ggggcagcct ttaccggtgg atgatgatac ggaagaagac
     1561 cgggagatag ccttgcgcct gcaagggcaa gaatcatttc tgacttcttc tacagaagaa
     1621 tatgcggcat gctttcatac ctttataaat gcattgcgtg ataatacttt tgataaatta
     1681 gtactttcac gtcatcttac tattgataaa gtatccggtt tttctccttt gtctatattt
     1741 cgtgcagcgt gcaggcgtta tattcattca tatatatact tgtgctacac tccgcagacg
     1801 ggtatctggc tgggaagcac tcctgaaatc attttatcag gtgaaaaaga cgaatggcat
     1861 acggttgcgt tagccggaac gcagccttta caagatggca gactgccgca agtgtgggat
     1921 gaaaagaacc ggaaagagca ggattatgtc gcttcttata ttcgtcgcca actcctatcg
     1981 ttgggcattc attctacgga gaacgggcct tatccggctt atgcaggtgc tttgtcacat
     2041 ttgaaaacag atttccggtt ttctttgaaa gataacaaag gtttgggaga tctcttgaaa
     2101 gtgttgcatc caacgcctgc tgtatgcggt ttaccgaaag aagaagccta tcggtttata
     2161 ttgcagaacg aaggttacga ccgccgttat tattccggtt ttatcggttg gctggacccg
     2221 gaagggcgaa ctgacatcta tgtgaatctg cgctgtatgc atattaaaga tgaacaactg
     2281 actctttatg ccggcggtgg actactggct tcttcggaat tgaatgacga atggctggaa
     2341 acggaaaaga agttgcaaac catcaaacgg ctgattgcaa cgcctccttt aaaatcataa
     2401 actactaata attatcatca tgtatacaga caagaagaac atactccagt tggttgcgtt
     2461 gctcgaggcg cacgggatta ctaaggttgt gttgtgtccg ggtagtcgta atacgcccat
     2521 cgtacatact ttgtctaatc atccgaactt cacctgttat gcagtgacgg atgaacggag
     2581 tgccggttat tttgccattg gtcttgcgtt gaatggtggt aagcccgctg ctgtgtgctg
     2641 cacttccggt actgcattat taaatcttca tccggctgta gcggaagcat tttatcagaa
     2701 tgttccttta gttgtcattt cagcagaccg tcccgctgcc tggatcggac agatggatgg
     2761 acagacagtg ccgcaaccgg gtgtgtttca aacgctggta aagaagtcgg tcaatcttcc
     2821 ggagattcat acggaagaag atgaatggta ttgtaatcgc ctggtgaatg aagctttgct
     2881 ggagacgaat catcatggaa aagggccggt gcatatcaat gttcctattt ccgaaccgct
     2941 gtttcaattc acggtagatg cactcccgga agtacgtgtg ataacccgtt atcagggatt
     3001 gaacgtctat gaccgtgatt ataatgatct ggtcgaccgg atgaataaat atcagaagcg
     3061 catgattatc atcggtcaga tgaaccttat ttatctgttt gaaaaaagat atatcaagtt
     3121 attatataaa cattttgcct ggttgacgga acatatcggc aatcagacag ttcccggtat
     3181 tccggtaaag aatttcgatg cggcacttta tgccatgccc gaagagaaga tagaccagat
     3241 ggttcccgaa ttgctgatta cttacggagg acatgtcgtt tccaaacgat tgaagaagtt
     3301 tctccgtcag catccgccta aagagcattg gcacgtatcg ccggatggtg aggtggtcga
     3361 tttgtacggc tcattgacta cagtgatcga gatggacccg tttgaatttc tggagaagat
     3421 cgccagcctg ctggataacc ggactcctga ctatccccgt gtatgggaga attattgtaa
     3481 gattattccc gaaccggatt ttgcctattc ggagatgttg gctgtcggta cattattgaa
     3541 agctttgccg gagtcctgtg cattgcatct ggcaaacagt tcggtagttc gttatgcaca
     3601 actatattcg attcctccta caattgaagt ttgttgcaat cggggaacaa gtggtattga
     3661 agggtcgttg tctaccgctg tcggttatgc ggcagcttcc gacaaactga atttcatagc
     3721 tatcggagat ctaagtttct tctatgatat gaatgcgtta tggaatatca acgtccgttc
     3781 caacctgcgt atccttttat taaataacgg aggaggagag attttccata ctttaccggg
     3841 attggatatg tcgggcacct cacataaatt tattgcagca gttcataaaa cgtcagccaa
     3901 aggctgggcg gaggaacgtg gtttccttta tctgcaggca caaaatgatg aagagctgac
     3961 tgaagcaatg aaaactttca cccagccgga aactatggaa caacctgtgc tcctggaagt
     4021 gtttaccaac aaaaacaagg atgcacgtat gttgaagaat tattatcatc aattaaaaca
     4081 aaaataaatt atgccaacac aaagagagtg gacaaccatc agagaatacg acgatattct
     4141 ttttgattat tataatggaa ttgcccgtat cacaatcaac cgtgaacgtt atcggaatgc
     4201 atttaccccg actaccactg ccgaaatgag tgatgcattg cgcatttgcc gtgaagaagc
     4261 ggatattgat gtgattgtaa ttaccggagc cggtgataag gcattctgtt cgggaggcga
     4321 ccagaatgtg aaaggacgtg gcggatatat cgggaaagac ggtgtgcctc gtttgagcgt
     4381 attggatgta caaaagcaaa tccgtagtat tccgaaacct gtgattgctg ccgtgaacgg
     4441 ctttgccatt ggtggcggac acgtgctcca tgtagtatgt gatttatcca ttgcttcgga
     4501 aaatgccatt ttcggtcaga caggtcctcg cgtgggtagt tttgatgccg gattcggtgc
     4561 atcttatctg gcgcgtgtgg taggacagaa gaaagcacgt gaaatttggt tcctttgccg
     4621 caagtataat gcacaagaag cattggatat gggattggtg aataaggtag tacctttgga
     4681 acagctggaa gatgaatacg tgcaatgggc ggaagaaatg atgttgctta gtccattggc
     4741 tttgcgcatg attaaagcgg gattgaatgc cgaactggac ggccaggcag gtattcagga
     4801 gttggcaggt gacgcaacat tactttatta cctgacggat gaagcacagg aaggaaagaa
     4861 tgcgttcctg gagaaacgta aaccgaactt caaacaatat ccgaaattcc cttaaaatcg
     4921 gctttgccac agagtaccac agagtttgct tttcctgcgt ttaagcctga ttttctgcaa
     4981 agcaagactg tggaggagag aatggtagga gaactgcaag agatataact tattaaaact
     5041 tcgtgaggct ctgtgttatt ctgtggtgaa ttgaaaaatt attcacgatg aattgtaaaa
     5101 tagaaattat tccccgattg ctccatttca agcaacctgc cgggacttcc agaggaacat
     5161 atactacgcg taaagtttgg tatcttcatt ttacctcgcc tgacttccct ggcagagtag
     5221 gaatagggga gtgtgcgcct ttgcccgctt taagttgtga cgatcttcct gattatgaag
     5281 atatattaaa aagattttgt cggcaagtag agaaagaaca aggaatgtgg gataaggatg
     5341 ttttatgcca gtatccttcc atcttatttg gcttggaaac ggctatctgg catttctttg
     5401 cagggagttg ggctttgtcc gatacggctt tttctcgtgg agaggtag
//
