LOCUS       c00670_gnlPro..         3088 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CKMDKBCG_670.
ACCESSION   c00670_gnlPro..
VERSION     c00670_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 15:26:20
            Original ID  :: gnl|Prokka|CKMDKBCG_670
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3088
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3088
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[326:3086]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      327..3086
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3088
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3088
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             327..2555
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg670_1"
                     /sec_met_domain="Gly_radical (E-value: 8.6e-48, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2571..3086
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg670_2"
                     /sec_met_domain="Radical_SAM (E-value: 2e-30, bitscore:
                     93.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDF"
ORIGIN
        1 cggagagaca gggattcgaa ccccgggtac ctcgcagtac aacggttttc aagaccgccg
       61 caatcgacca ctctgccacc tctccaaaac ttctttatca gaagtgcttt tcgttaaaag
      121 cggtgcaaag gtacgaatat attttaaact tacaagagtt ttcacaaaaa aaaatcacga
      181 acatcatttc caaagaatag caatatctag gtattttatg ttgaaaagca taaaatagag
      241 catatttatg ctgattatat gaaaaaacgt tgtaactttg tgcgctctaa agaaaaggat
      301 aactacaacc tctaatttta tcatttatgg aattaaataa gatctttaaa gacggtcttt
      361 ggagcactga aatcaatgtc agagatttcg taagtcataa catcactccg tattatggag
      421 atgcttcttt cctcgaagga cctaccgaac gcactaaagc tgtatggaac cgctgcctcg
      481 aagcactggc agaagaaaga gccaataacg gagtacgctc actggataat gtcaccgtct
      541 caaccatcac ttctcataaa gccggatata tcgacaaaga aaacgaactg atcgtcggtc
      601 tgcagacaga cgaacttctg aaacgtgcca tcaagccttt cggaggtatc aacgtagtga
      661 gcaaagcctg ccacgaaaac ggcgtggaag tggacgaccg cgtaaaagat atcttcactc
      721 actaccgcaa gacgcacaac gacggagtat ttgacgtata taccgaagaa atccgctcgt
      781 tccgttctct gggattcctc accggacttc ctgacaacta tgcccgcgga cgtatcatcg
      841 gcgactaccg ccgtatggct ctctatggta tcgaccgatt gattgaggca aaaaaagaag
      901 atttgcgtaa ccttaccggt ccgatgaccg aagcccgcat ccgcctgcgc gaagaagtgg
      961 cagagcaaat caaggcattg aaggacatga aggtaatggg cgaatactac ggactcgact
     1021 tgagccgtcc tgcctacacc gcacaagaag cagtacaatg ggtgtacatg gcttaccttg
     1081 ctgccgtaaa agaacaagac ggtgctgcca tgtcactggg taacgtctct tcgttcctcg
     1141 acatttatat ggaatatgaa ttgagcaaag gaaccatcac agaatctttc gcacaggagc
     1201 tgatcgacca gtttgtcatc aaactgcgta tggtgcgtca cctgcgtatg caatcttaca
     1261 acgacatctt tgccggcgac ccgacatggg taaccgaatc cctgggtgga cgcctcaacg
     1321 acggacgcac gaaggtgaca aagacttctt tccgcttcct gcaaacactc tacaacctcg
     1381 gcccttcacc ggaaccgaac ctgactgtat tgtggagccc ggaactgccg gaaggattca
     1441 aagaattctg cgcaaaagta tctatcgaca cttcttccat ccaatatgaa aacgacgacc
     1501 tgatgcgtga agtgcgccag tctgacgatt acggaatcgc atgctgcgta tcttatcagg
     1561 aaatcggaaa acaaatccaa ttcttcggcg cacgttgcaa cctggcaaaa gccctgttgc
     1621 ttgccatcaa cggcggacgt tgcgaaaaca ccggtacggt aatggtgaaa aatatccccg
     1681 tactaaccag cgacacactg aacttcgaag aagtaatgag caactacaag aaggtattga
     1741 cagaaatcgc ccgtgtttac aacgaagcga tgaacatcat ccattatatg catgataagt
     1801 attactacga aaaagcccaa atggctcttg tagataccaa cccacgcatc aaccttgctt
     1861 acggtgtagc cggactttct atcgccctcg attcactgtc ggccatcaaa tatgctaaag
     1921 taaccgcacg ccgcaacgac atcggtctga cagaaggttt cgatattcaa ggcgaattcc
     1981 cttgcttcgg taatgacaac gacaaggtag accacctggg tgttgacctg gtctatttct
     2041 tcagcgaaga attgaagaaa cttccggttt acaaaaatgc ccgtcctact ttatctttgc
     2101 tcaccatcac ttccaacgtg atgtatggca agaagacagg tgctactccc gacggacgcg
     2161 ccaaaggtgt cgcttttgct ccgggagcca accctatgca cggacgtgac aagaacggtg
     2221 ccatcgcctc tttgagttcc gtagcgaaat tacgttaccg cgactcacag gacggtatca
     2281 gcaacacttt ctccatcgtc cccaaatcac tgggagctac tgaagaggat cgcgtagaaa
     2341 atctggtgac aatgatggat ggctatttca ccaaaggcgc tcaccacctg aacgtgaacg
     2401 tactgaaccg tgaaatgctt tacgatgcca tggaacatcc ggaaaaatat ccgcaactca
     2461 ccatccgtgt ttccggttat gccgtaaact tcgtgaagtt gagccgcgaa catcaattgg
     2521 aagttatcag ccgtagcttc cacgaacgta tgtaatcaga tctactcaat atgacgataa
     2581 acgtacattc atacgaaagt atgggaacat tcgacgggcc gggcttacgg ctcgtcgttt
     2641 ttcttcaagg atgcaatttc cgctgcctgt attgtgccaa ccccgacacg atagccggaa
     2701 aaggaggcac acctacccca ccggaagaaa tcgtccgcat ggctatgagc caacgtcctt
     2761 ttttcgggaa acgcggagga gtcactttct ccggaggaga acctacgttt caggccaaag
     2821 cactagtccc tttagtgcgt gaactgaaag aaaagggaat acatgtatgc atagacagca
     2881 acggcggtat ctggaacgaa gaagttgaag aacttttcaa actgacggat cttgtattgc
     2941 tggatattaa agagttcaat ccggcacgcc atcaagcact gaccggaaga agtaacgagc
     3001 aaaccatccg cacggcagca tggctggaag aaaacgagaa acctttctgg ctgcgttatg
     3061 tattagtgcc cggatacagc gatttcga
//
