LOCUS       gnlProkkaHEBFBKGL_574   3218 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HEBFBKGL_574.
ACCESSION   gnlProkkaHEBFBKGL_574
VERSION     gnlProkkaHEBFBKGL_574
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 07:49:26
            Original ID  :: gnl|Prokka|HEBFBKGL_574
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3218
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..276)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg574_1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 8.6e-15, bitscore:
                     43.2, seeds: 75, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRPEFFVNILKEQGIDFYAGVPDSLLKNICAYITDHLPAEQNII
                     AANEGGAMGIAVGYHLATGKVPVVYMQNSGEGNIINPFASLTDKDVY"
     protocluster    1..3218
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[0:2769]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..2769
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..3218
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3218
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(311..1621)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 190.9;
                     E-value: 4.6e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg574_2"
                     /sec_met_domain="CTP_transf_like (E-value: 5.1e-13,
                     bitscore: 37.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 5.9e-115, bitscore:
                     371.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 3.7e-62, bitscore:
                     198.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTVYLGMIGDIMHPGYINIINKATEYGDVIIGLFTDKAIANHR
                     RLPYLTWEQRKNVVESIRNVSRVVPQDDWSYVSNLLKYKPDYIIHGDDWQVGPDKYIRD
                     EVFKVMEKLGGEVIEIPYTQNISASGIKQEIDALGVTPQMRLSSLRRLIAAKPIVRILE
                     SHNGLTGLIAEHTKVEVNGQEREFDGMWASSLTDSTSKGKPDIEAVDLTTRLHDLNDTL
                     EVTTKPVIFDGDTGGKIEHFGFTVRTLERLGISCVIIEDKVGLKQNSLFGTDAVQTQDT
                     IEGFQAKIRAGKEAQITRDFMIVSRCESLIAGKTVDDALERCHAYVEAGTDGIMIHSKE
                     KSGEDIKEFCLRFREKNAHTPIIVVPTTYNQFTEEELAEWGINVVIYANHMLRSAYPAM
                     VKCAETILYTSRSLEASEQYCMPIKQILNLIPGTKKK"
     CDS             complement(1646..2020)
                     /locus_tag="ctg574_3"
                     /transl_table=11
                     /translation="MTYRQVGTNSFTVKYYVEKFILDMNTMKIIRVDEYRDKKKINRPA
                     GSLFSVDGEIYRVAQKCSRAYGESIFVYKTSKNFDFIKDKKVAELTGQSIVLSDGRKPI
                     LLHTYSQAGGIEVIDYRCSL"
     CDS             complement(2017..2769)
                     /NRPS_PKS="Domain: Aminotran_5 (3-189). E-value: 1.1e-20.
                     Score: 65.7. Matches aSDomain:
                     nrpspksdomains_ctg574_4_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg574_4"
                     /sec_met_domain="Aminotran_5 (E-value: 4.4e-19, bitscore:
                     57.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMLENERLVKKFANATKDSKVVFMTCSGSGGMEAAIINCLTPQDK
                     ALVINGGSFGERFVELLTLHKIPFTEIKLKYGRALKPEHLAEYESKGYTTFLMQKHETF
                     TGVHYDINLVSDFCKRNNCFLIVDIISTFLCDSFDMVAMDVGVMITGSQKALACAPGIA
                     VMLLAPSAIKRIEKVQCCCQYLDLKLALKNMERGQTPCTPAVGILRQINVRLKEIESAG
                     GAEVEIARCAELAKYFRDKLVKNNLPLF"
     aSDomain        complement(2203..2760)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg574_4_Aminotran_5.1"
                     /evalue="1.10E-20"
                     /label="ctg574_4_Aminotran_5.1"
                     /locus_tag="ctg574_4"
                     /protein_end="189"
                     /protein_start="3"
                     /score="65.7"
                     /tool="antismash"
                     /translation="ENERLVKKFANATKDSKVVFMTCSGSGGMEAAIINCLTPQDKALV
                     INGGSFGERFVELLTLHKIPFTEIKLKYGRALKPEHLAEYESKGYTTFLMQKHETFTGV
                     HYDINLVSDFCKRNNCFLIVDIISTFLCDSFDMVAMDVGVMITGSQKALACAPGIAVML
                     LAPSAIKRIEKVQCCCQYLDLKL"
     CDS             complement(2766..2867)
                     /locus_tag="ctg574_5"
                     /transl_table=11
                     /translation="MINFTVGPVQSSDAVRVIGAEQVPYLEQQSFQK"
ORIGIN
        1 ataaacgtcc ttgtcggtaa gagaggcaaa cggattaatg atattgcctt caccagagtt
       61 ctgcatataa actacaggca ccttacctgt tgccaaatga taacctacag caatacccat
      121 ggcccctcct tcgttagcag caataatatt ctgctcagca ggaaggtggt cggtaatata
      181 tgcacaaatg ttcttgagca acgaatcagg aacaccagca taaaagtcta taccttgttc
      241 cttgagtata tttacaaaaa attcgggtct taccatttgt aaaatagttt aaggggtaag
      301 aaattaatgt ttacttcttc tttgtgcctg gaataagatt aagaatctgt ttgataggca
      361 tacagtactg ctcagatgct tcaagcgaac ggctagtata gaggatggtt tctgcacatt
      421 taaccattgc aggataagca gaacgaagca tgtgattagc ataaatcact acattaatac
      481 cccattcagc aagttcctct tctgtgaact ggttataagt agtaggcaca acgatgatag
      541 gagtatgagc attcttctcg cgaaagcgca agcaaaactc cttgatgtcc tctccagact
      601 tctctttgga gtggatcatt ataccgtcag tacctgcctc tacgtaagca tgacaacgct
      661 ccaaagcatc atctacagtc ttaccagcaa tgagagactc acaacggctg acaatcatga
      721 agtcacgagt aatttgcgct tccttacctg cacgaatttt agcctgaaaa ccttcgattg
      781 tatcctgagt ctgtacagca tcagtaccaa aaagtgaatt ctgttttaaa cctaccttat
      841 cttcaattat aacgcaagag attcccaagc gctcaagggt gcgaacggta aaaccgaagt
      901 gttcaatctt gccaccagta tcgccatcga agataacagg ttttgtggtt acttcaaggg
      961 tatcattgag gtcatggaga cgggtagtca aatcgacagc ctcaatatca ggcttaccct
     1021 tagaggtaga gtcggtaagg gaggaagccc acatgccatc gaattcacgc tcctgaccat
     1081 tcacctctac cttagtatgt tctgctatga ggccagtaag accattgtga ctttcaagaa
     1141 tacgtacaat cggtttagct gcaataaggc gacggagaga agaaagacgc atctgaggag
     1201 ttacaccaag agcatcaatc tcttgtttga taccagaagc agagatgttt tgggtgtaag
     1261 gaatctcgat aacctcacca cccaactttt ccattacttt gaatacctca tcacggatat
     1321 acttgtcagg acccacctgc caatcatcac catgaatgat gtaatcaggc ttgtacttaa
     1381 ggagattaga gacatagctc caatcatcct gagggaccac ccttgatacg ttcctgatgc
     1441 tctcaacaac gttctttctt tgctcccaag tgagataagg aagtctacgg tggttagcaa
     1501 tcgccttatc agtgaacaaa ccgattatca catcgccata ctcagtagct ttgttaatga
     1561 tgttaatgta acccggatgc atgatatcac ctatcatacc aagataaact gtctttgcca
     1621 ttattattta acatttttca aattattata aagagcatct atagtcaatc acctcaatcc
     1681 ctcctgcttg agaataagtg tgcaacaaaa tcggctttct accatcactc aggactatac
     1741 tctgaccagt gagttctgct acctttttat ctttaataaa atcaaaattc ttacttgtct
     1801 tatacacaaa gatcgactct ccatatgcac gactgcattt ttgtgccact cgataaattt
     1861 ctccatctac agagaataac gaacctgcgg gacgattaat cttctttttg tcgcgatact
     1921 cgtcaaccct aattatcttc atcgtattca tgtcaagaat aaacttctca acataatact
     1981 taactgtaaa gctatttgtt ccaacctgac gataagtcaa aagagtggaa gattattctt
     2041 aacaagctta tcacgaaaat acttagccaa ttctgcacaa cgcgcaatct ccacctccgc
     2101 accacctgca gactcaattt cttttaaacg gacattaatt tgacgaagga taccaacagc
     2161 aggagtacaa ggagtctgcc ctctctccat atttttaaga gcaagcttca aatcaagata
     2221 ctgacagcaa cattggacct tttctatacg tttaatagcc gaaggagcaa gaagcataac
     2281 tgcaataccc ggagcgcaag caagagcctt ctgacttcca gtaatcatca cccctacatc
     2341 cattgccacc atatcaaatg aatcgcaaag gaaggtagag attatatcaa caatcaagaa
     2401 acaattatta cgtttgcaaa aatcagaaac aagattgata tcgtaatgta cccccgtaaa
     2461 agtttcgtgc ttctgcataa ggaaagtagt gtaacccttg ctctcatatt cagcaagatg
     2521 ttcaggcttc aaagctctac catacttaag cttaatttcc gtaaaaggaa ttttatgaag
     2581 ggtaagcaat tccacaaaac gctcaccaaa gctaccacca ttgattacaa gagccttatc
     2641 ctgtggcgta aggcaattta tgatggcagc ctccatacca ccagagccag aacatgtcat
     2701 gaaaacaacc ttactatctt tagtcgcatt agcaaatttc ttaacaaggc gctcattctc
     2761 aagcatcatt tctgaaaact ctgctgttct aaatatggta cctgttcggc acctatgacc
     2821 cgtacagcat cagaagattg caccggacct actgtaaaat taatcattta tatagattcc
     2881 tttaattatc agtaataatc aaccaatcca atcaccactc ccccttcttc ctaacctcca
     2941 gccatttttc cacccattcc ccagccaatt tcctgatttc gtcatcatcc agttcctcca
     3001 gttcatcgat atagtatcgc agaagagttt cgtatgtact aagtgaggcg ttacaccctt
     3061 ttttttaagt ctatgtacgt ggatggagaa atatgcaaat ccttgcaaag agtttcaata
     3121 gtgactccac tctgttttct cgttcgctcg ataaaaaagc ccaatgaagg gtgaataaaa
     3181 tttttgccca tttagataaa ttttaagtaa ttagatag
//
