LOCUS       c00273_gnlPro..         6652 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JJOMKGMG_273.
ACCESSION   c00273_gnlPro..
VERSION     c00273_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:33:02
            Original ID  :: gnl|Prokka|JJOMKGMG_273
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6652
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6652
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1421:4377]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1422..4377
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6652
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6652
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..509)
                     /locus_tag="ctg273_1"
                     /transl_table=11
                     /translation="MKSTFNICFYAKKDKQKANGAYPLFARITVDGVASRFNTKLDVLP
                     SIWDGKMGKATGRTSEASRINRMLDDINASLNTIYHEMQRRDNYVTAEKVKNEFLGHSE
                     SHETILTLFQKHNDDVKQLVGISKTIATYRKYEVTRRHLAEFIQSKYNVSDISIKEISP
                     MFITDF"
     CDS             1422..3650
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_2"
                     /sec_met_domain="Gly_radical (E-value: 2.8e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             3652..4377
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_3"
                     /sec_met_domain="Radical_SAM (E-value: 7.4e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(4446..6422)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 84.8; E-value: 1.1e-26)"
                     /gene_kind="other"
                     /locus_tag="ctg273_4"
                     /sec_met_domain="Fn3-like (E-value: 8.9e-28, bitscore:
                     84.8, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.1e-71, bitscore:
                     230.1, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3e-53, bitscore:
                     169.2, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNVIGVKDIRRYQELALQSRLRIPLIFGLDVIHGYRTGFPLPLA
                     EAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARWGRVMEGAGEDPYYGSRV
                     AEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVDMSMGHFANFYMPPYKAA
                     IKAGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVVSDYNSVEELVNHRYAVD
                     KKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDDAVRRILEKKYELGLFED
                     PFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLPLTAQTRKIALIGGLSKS
                     QYDMAGAWAATTDRNKVVTLYDALVRKGLEVSYAEGYDMKSNQLTGLQEALQAAEASDV
                     IVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNEIREH
                     ADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALK
                     QTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKAAGVHTEVSVKVTNTGKCAGE
                     EVVQLYVQDKVASVTRPIKELKGFRKIHLNVGESKTVAFQITDDALGFYDNEMNYMVEP
                     GDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(6438..6650)
                     /locus_tag="ctg273_5"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVR"
ORIGIN
        1 tcaaaatcgg taatgaacat cgggcttatc tccttaatgg atatgtccga tacgttgtac
       61 ttgctttgaa tgaactcggc aaggtggcgg cgggtgactt catacttgcg gtaagtcgct
      121 attgtcttgg atatgccgac caactgcttc acatcgtcat tgtgcttttg gaacagggta
      181 aggattgttt cgtggctctc gctatgacct aaaaactcgt tcttcacttt ctcggcggtc
      241 acgtagttgt cacgccgttg catttcgtgg taaatggtgt tcaaggatgc gtttatatcg
      301 tccagcatac ggtttatgcg gctggcttcc gaagtacgtc cggtagcctt gcccattttg
      361 ccgtcccaaa tagagggcag cacgtccagc ttggtattaa aacggctcgc cacgccgtcc
      421 actgtgatac gggcgaaaag ggggtacgca ccgttggctt tctgcttgtc tttctttgcg
      481 taaaagcaaa tgttgaatgt cgatttcata actcactttt taaattgcaa aactattgta
      541 attctctaaa aatgagttct atgcaggcaa gccaaatagc gacaggactt cgccaatttc
      601 ggacaatctg tacccccttt ccgtttttgt ttgtcggggg tacgagttag gttacgaact
      661 ttgtcttttt ggggcgaaaa ggtgtacttt aaggcagaca caagggaata aaaaaagtcc
      721 cacaaatcat tgaatttgtg ggacttgtct gcttactgtc cggtattgtc cggttagttt
      781 tgcggagaga cagggattcg aaccccgggt gcctcgcagc acaacggttt tcaagaccgc
      841 cgcaatcgac cactctgcca cctctccaaa acttcttttt cagaagtgct tttcttttaa
      901 agcggtgcaa agatacaaat cattttttat tctacaaact tttctgcaca aaaacagagc
      961 aattatacca aaattcttgt ttatacccac tacggaacga acataacggc atttaggcta
     1021 tttgttgaaa tttatcaagg gcaagacaga gttttataac ttactggcta tcagtatatt
     1081 tacaaagagc cacattcagt atacgattga accctattca gtatacgact gaatggcatt
     1141 cactatacga ctgaattagg ttcagaatac gactgttcct taaacctaca tcatttgtaa
     1201 ataatgcaga ttattattcg gttcttgttt caattcataa accacatata aagaaagtat
     1261 taacatctct actcccccgg acattcacta cctaataata gttattttat gctgttcaac
     1321 ataaaaagag aagtatctct tataaaatta cggaatttat tcagtatttt tgcgcgctct
     1381 aaaggataac attatctaac ctactaacaa aaacaaattt tatggaattg aacaaaacct
     1441 ttaccaatgg gctttggaat aaagaaatca atgtcagtga tttcgtcagt aaaaacatcg
     1501 caccctatac cggggatgca tctttcttgc agggacctac cgaacgtacc aaacgtatct
     1561 gggacatctg tctgaaggct ctcgaagaag aaaggaacaa caatggcgtc cgttcactgg
     1621 acaataccac cgtatctacc atcacttctc ataaagccgg gtatattgat aaggacaatg
     1681 aacttattgt cggtttgcag actgatgaac tgttgaaacg atccatcaaa cctttcggag
     1741 gcatcaacgt ggtagcaaaa gcatgccgtg aaaacggagt ggaagtggac gataaagtaa
     1801 aagacatctt cacccattac cgcaaaacac ataacgacgg cgtattcgat gtatataccg
     1861 aagaaatccg ttccttccgc tcactgggat tccttaccgg attgcccgac aactatgccc
     1921 gcggacgtat catcggcgac taccgccgat tggctctcta cggcatcgac cgtctgattg
     1981 aagccaagca ggaggacttg cgcaacctca ccggcccgat gaccgaagca cgcatccgtc
     2041 tacgcgaaga agtggcagaa caaatcaaag ctttgaagga catcaaggtt atgggcgaat
     2101 attacggact cgacctcagc catccggcta catcagctca agaggccgtg caatgggtat
     2161 acatggcata tcttgctgcc atcaaagagc aggacggagc agccatgtca ctgggtaacg
     2221 tatcttcgtt cctcgatata ttcattgaat acgatctggc tcacggaaaa atcaatgaga
     2281 cgtttgcaca ggaactgata gaccagttcg tcatcaagct gcgcatggta cgccatctgc
     2341 gtatgcaatc atacaatgat atctttgccg gtgacccgac ttgggtgacc gaagccatcg
     2401 gaggacgctt caatgacgga cgcgtgaagg tcaccaagac ttctttccgc tttttgcaga
     2461 cgctatacaa cctcggtcct tctccggaac cgaacttgac cgtattgtgg agtcccgaac
     2521 tgccggaagg cttcaaggat ttctgcgcca aagtttctgt agataccagc tccatccagt
     2581 acgaaaacga caacctgatg cgtgaagtgc gcaactgcga cgattacggc attgcctgct
     2641 gtgtatccta ccaagccatc ggcaaacaaa tccagttctt cggcgcacgt gccaaccttg
     2701 ccaaggccct gctgctcgcc atcaacggtg gacgttgcga aaataccgga actgtcatgg
     2761 tgaaaggtat tcccgtattg accggtgaga cattgaagtt tgaggaagtt atggccaact
     2821 acaagaaggt actgactgaa atagcccgtg tctacaacga ggccatgaat atcatccact
     2881 acatgcatga taagtattat tatgagaaag cccagatggc attcatcgat accgacccac
     2941 gcatcaatct ggcatacggt gttgccggcc tttccatcgc catagactca ctgtccgcca
     3001 tcaaatatgc caaagtcact gcacgccgca acgaaatcgg cctgaccgaa ggtttcgaca
     3061 ctgaaggttc attcccctgc ttcggcaata atgacgaccg tgtggaccac ctgggagtag
     3121 acctcgttta ctatttcagc gaagaactga aaaagctgcc tgtctacaag aatgcccgtc
     3181 ccaccctttc cttgctgact atcacctcca acgtgatgta cggcaagaag accggtgcca
     3241 ctccggacgg acgcgccaaa ggtgttgcct ttgctccggg tgccaaccca atgcacggac
     3301 gtgacaagaa cggtgccatc gcttccttga gctccgtagc caaattgcgc taccgcgact
     3361 cgcaggacgg catcagcaat acgttctcca ttgttcccaa atcactgggc ccgacaccgg
     3421 aagaacgtgt agagaacctg gtaaccatga tggacggcta cttcaccaaa ggtgcccacc
     3481 atttgaacgt aaacgtactg aaccgcgaaa tgctggaaga tgccatggaa catcctgaaa
     3541 agtatccgca actcaccatc cgcgtttccg gctatgccgt gaacttcgtg aagttgagcc
     3601 gcgagcatca gttggaagtc atcagccgtt cattccacga aagaatgtaa tatgataaat
     3661 gtacactcct acgaaagtat gggaacattc gacgggccgg gtctccggct cgtcgttttc
     3721 ctgcaaggat gtccgttccg ttgtctgtat tgtgccaatc cggatacgat tgatgtaaaa
     3781 ggaggcactc ccaccactcc ggaagagatt ctgcaaatgg ctatcagcca aaaggcattc
     3841 ttcgggaaga aaggaggtat tactttctcc ggcggtgagc ctaccctgca agcagaagcg
     3901 ctgattcctc ttttcaagga gctgaaggcg aacggcatcc atatctgtct ggacagtaac
     3961 ggaggcatct ggaatgaaaa ggtggaagag ctctttagcc tgactgacct cgtgctgctg
     4021 gacatcaagc aattcaatcc cgaacgccac cgtacgctga cgggacgaag caacgaacag
     4081 acactccgca ccgctgcctg gctcgaagaa catgaacacc ccttctggct gcgctatgta
     4141 ctggtccccg gttacagcga ttcggaagaa gacacccgcc tgctgggtga aagtcttggc
     4201 aaataccagc agatacaacg ggtggaaata cttccttatc accgtctggg tgttcacaaa
     4261 tacgaagcca tgggatggga ttacaaattg aaagaagtga aggaaaatac tccggaacaa
     4321 ctggaacggg cagaaaaact gttcaaagag tatttcacga atgtgataac caactaaaac
     4381 agaaaagcaa ggctgtccgg atatgattcc agacagcctt gcttttcatt tatagggtaa
     4441 gaatcctatt ctttcaagtt ataggtaatc tgctgtacat catcactact gccacccacc
     4501 ataaagacga aatcaccagg ttctaccata taattcatct cattgtcata gaatcccagt
     4561 gcatcgtcag ttatctgaaa agctacagtt ttgctttcac ccacattcag atgaattttc
     4621 ctgaagccct tcaattcctt tatcggacgc gtaacagagg ctaccttatc ctgcacatac
     4681 aattgaacta cttcctctcc tgcacactta ccggtattag tcacttttac cgacacctcc
     4741 gtatggacgc ctgcagcctt gcccggcagc agtttcaaat cagaatatcc aaaatccgtg
     4801 tacgaaagcc catatccgaa aggataggca ggctcagtgg aagcatcgat ataagatgcc
     4861 ttataggtct gtttcaatgc ggtagggcgg ccggtgctct tattctgata ataaatggga
     4921 atctgtccta cgtgtgcagg aaatgtcatg ggcaatttag ccgaaggatt atactctccc
     4981 cacaacacat tgcatatggc attgccggcc tcacttccca gccaccaagt acacagtacc
     5041 gcatcagcat gttcccttat ctcattgaag attaccggac gcccacacat gacaagagca
     5101 atgacaggtt ttccagtctt tgccagttcc gacaccagct tctgctgccc ctcgggtatg
     5161 gctatattca ctttggaagc cttctcgcca ctctgaccga atctctcgcc taccgccaca
     5221 acaatgacat ccgaagcctc agcagcctgt aatgcttcct gcaatccggt caactgattg
     5281 ctcttcatat catagccttc agcatagctc acctccagtc ctttccggac caatgcatca
     5341 tataaggtca ccactttgtt ccggtccgta gtggcagccc aagcacctgc catatcgtac
     5401 tgggattttg agagtcctcc tatcaaggct atcttcctcg tctgtgccgt caacggaagc
     5461 acagattccc tattctccag caatacaacg gaacgctcgg ccatacggag cgaagcatta
     5521 cggacttcgg gcgcattgag tattctggcc gctctttccg gattacagta gcgaaaaggg
     5581 tcctcaaaca atcccaactc atatttcttc tccaatatac gccgtactgc atcatcaagc
     5641 acactctcct ttactttccc ttcttgaaca agctccttca gataagtcaa gtagcaggta
     5701 cttaccatct ccatgtccag accagcattc agcgctttgg ctgcggcatc tttcttgtca
     5761 acagcataac ggtggttcac taattcctct accgaattat aatcggacac aacaaatccc
     5821 ttaaatcccc actgctctct caatagccgg ttgagcaaga aatcattggc agtacaagga
     5881 atattattaa aatcattgaa agcactcatc acggtagctg ctcctgcctt tatggccgcc
     5941 ttatagggag gcatataaaa attggcaaag tggcccattg acatatccac actattatac
     6001 tcctttcctg ctatcggtgc accatatccg gcaaaatgtt tcatacatgc catgacagtc
     6061 gtcgtgtctg ccaaatcgtc tccttgcaag cctctgacac gcgcctccgc tacccgagaa
     6121 ccataataag ggtcctcacc ggccccttcc atgacgcgtc cccaacgtgc gtcccacgaa
     6181 atatccacca tcggggcaaa aacccagttc aaaccatcgg cggcactttc caaagcattg
     6241 tagcgtgcag cttcctcaat catggccaaa tcgaaacttg cagcctcggc aagcggaagc
     6301 ggaaatccgg tacgatagcc gtggataaca tccagtccga aaattaaagg aatgcgcaaa
     6361 cgcgattgca aagcaagctc ttgataacgg cgtatatcct ttactcctat cacattcagc
     6421 atagagccta ttctgcctta ccggacatac tcttctttag gaatatttcc accgtcgggt
     6481 ccggtaaaat tccctcggta ggtgcattgt gcaagctggc ctatcttttc atccagcgtc
     6541 atctgcttca tcaagcttga gatgaatttc tccttctccg tagtttgtgc cgaggtaaag
     6601 gtgttaccaa gtccccacag acaaaccatc agtattatta tctttttcat ac
//
