LOCUS       c00473_gnlPro..         3364 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KLCMFMLH_473.
ACCESSION   c00473_gnlPro..
VERSION     c00473_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 11:29:15
            Original ID  :: gnl|Prokka|KLCMFMLH_473
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3364
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3364
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[459:3362]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      460..3362
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3364
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3364
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             460..2688
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg473_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
     CDS             2757..3362
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 78.5;
                     E-value: 1.9e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg473_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.1e-29, bitscore:
                     90.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGV"
ORIGIN
        1 gagtaaaggt gttttttaga ctttcaattt tactacataa aacatatgat acttcttcat
       61 agaaattaaa ttccgattta taatagtaat agcataaaaa aaatcatttt ttattttgca
      121 gattaaaaac aaacttctaa ctttgcaccg tcttagaaaa agaattaaga ctaactgatg
      181 gcggtatcgt ctagtggcct aggacgcagc cctctcacgg ctgaaactcg ggttcgattc
      241 ccggttccgc tacttttaga agctaattgg tttattgtca ccaattagct ttatttttta
      301 tggaattgtt ataaagagtc cttattgttt tttcatttat tgaccacttt atacttaaaa
      361 atgcacaagt attcgcatta tacctactaa tagtgatgca tagctcacaa aaaaggagta
      421 cctttgcagc cctaaatctc ttaataatgg aataacagta tggaaatgaa caaagttttt
      481 aaggatggat tgtggagtaa ggaaattaat gtttcagact tcgtacatac caacattacc
      541 ccttatgagg gcgatgcctc atttctggca ggccccacag agcgaactaa aaaagtgtgg
      601 aacgaatgcc tcaaggcact agaagaagaa cgtgccaata acggagttcg ttcccttgat
      661 aatgtaacag tatctaccat cacctcacat aaagcaggat acattgaccg tgaaaacgaa
      721 ctgattgtag gtcttcaaac ggatgaactt ttgcgccgtg ccatcaaacc ttatggtggt
      781 atcaaagtcg tagaaaaagc ctgccatgaa aacggtgtgg aagtagatga aaaagtaaag
      841 gatatcttta ctcattatcg caaaacccat aatgatggcg tatttgatgc ttacaccgaa
      901 gaaatccgct ctttccgttc actcggtttc ctgaccggtc tgcccgataa ttatgcacgc
      961 gggcgtatca tcggtgacta ccgccgtttg gcactgtatg gtatcgaccg actgattgaa
     1021 gcaaaacaag aagatttgca caatatcacc agtccgatga ccgatgcccg tatccgcctg
     1081 cgcgaagaag tagccgaaca aatcaaagca ctgaaagaaa taaagatatt gggtgaatat
     1141 tacggactcg acttgggtcg tcccgctact aatgcacaag aagctgttca gtgggtttat
     1201 atggcatatc ttgctgccgt gaaagaacaa gatggagcag ctatgtcatt gggcaacgtt
     1261 tcttcattcc tggatatcta tattcaatat gatttggatc atggtaaaat tgacgagtca
     1321 tttgcgcagg aactggtaga ccagttcatt atcaagttgc gtatggtccg tcatctgcgt
     1381 atgcaatcat acaacgatat ctttgcaggt gatcccacct gggtaaccga gtctatcggc
     1441 ggacgcttca acgacggacg taccaaggtt acaaaaactt ccttccgttt cttgcagaca
     1501 ctatacaatc tgggtccgtc acccgaacct aatatgactg tactgtggag tccggaattg
     1561 ccggaaggtt tcaaagaatt ctgtgcacaa gtttctatcg acacttcttc tatacaatac
     1621 gaaaacgaca cgctgatgcg cgaagtgcgt aactgtgatg attatggtat cgcctgctgc
     1681 gtttcttatc aggctatcgg caaacaaatc cagttcttcg gagcacgttg caatctggct
     1741 aaagccctat tgctggctat caatggcgga cgttgtgaga atacaggaac tgtaatggtg
     1801 aaagatattc ctgtattgac aggggacttg ctgaactttg aagaagtatg gtctaattat
     1861 aagaaagtat taatgcaaat tgcacgtgtg tataatgatg cgatgaatat cattcactat
     1921 atgcacgata aatattatta tgaaaaggct caaatggcat tcattgatac cgatccacgt
     1981 atcaatctgg catatggcgt agccggtctg tccattgcca ttgactcatt gtctgccatc
     2041 aaatacggca aagtacgtgc caaacgtaat gatataggtt tgaccgaagg ctttgatatt
     2101 gaaaatgcat atccttgctt cggtaatgat gatgaccgcg tagaccactt aggggtagat
     2161 ttggtttatt tcttcagtga agaattaaag aaacatccgg tatacaaaaa tgcccgccct
     2221 accctgtcac tgctgactat cacctccaat gtaatgtacg gcaagaaaac aggtgccact
     2281 cctgatggtc gtgccaaagg agtggctttc gctccgggtg ccaacccgat gcacggacgt
     2341 gacaagaacg gtgccatcgc ctcattgagt tctgtagcca aactacgcta tcgtgatgcc
     2401 caggacggca tcagtaacac gttctctatc gtaccaaaat cactaggggt agaccaggaa
     2461 acccgtatag agaacctggt aaccatgatg gacggttatt tcaccaaagg agcacatcat
     2521 ctgaatgtaa atgtgctgaa ccgtgaaatg ttggaagatg caatggaaca tcctgaaaaa
     2581 tacccgcaac taaccatccg cgtatcaggt tatgccgtaa acttcatcaa attgagccgt
     2641 gaacatcagc tggaagtaat cagccgttct ttccacgaaa gaatgtaaca tcacatatac
     2701 attcatcgta ggggcgggtt ttaaacctgc ccttaccagt ttaaagacaa accattatga
     2761 tcagagtcca ttcttacgag tcaatgggga cgtttgacgg tcccggttta cgcttagtcg
     2821 tatttttaca aggatgcaat ttccgatgcc tctattgtgc caaccccgac acaattgaca
     2881 ccaaaggtga aagtacggaa acaacaatag acgaaattgt gcgtatggcc gtcagccaaa
     2941 aagctttttt cggtaaaaaa ggaggtgtga ctttcagcgg cggcgagcct acattgcaag
     3001 caaaagcact gattccgtta ttccagcgat taaaagaaca aagcatccac atctgtatag
     3061 ataccaacgg cagtatatgg aatgaagaag taaaggaact gctacaatgg acagacctcg
     3121 tattattaga catcaaggaa tttaataatg tgcgccatcg gcaactgaca gaacgcagca
     3181 acgaacaaac catacgtacc gccggatggc ttgaaaaaaa cggaaagcct ttttggttgc
     3241 gctatgtatt agttccaggc tatagttcct ttgaggaaga tatacgggca ttaggagagc
     3301 acttcaagaa ttatcaaatg attcaaagag tagaaatact accttatcat acattaggcg
     3361 taca
//
