LOCUS       gnlProkkaODKNMFEK_584  13007 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ODKNMFEK_584.
ACCESSION   gnlProkkaODKNMFEK_584
VERSION     gnlProkkaODKNMFEK_584
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 08:01:40
            Original ID  :: gnl|Prokka|ODKNMFEK_584
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13007
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13007
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[965:8477]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      966..8477
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..13007
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13007
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /tool="antismash"
     CDS             complement(9..962)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg584_1"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.2e-07, bitscore:
                     21.4, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCPVYINRIASIHAESSNEKPYFSAKEPDYKEMITNANLRRRMSR
                     MIKMGVACGLECLKDISPEKVDAIITATGLGCLADTEKFMNALMDNREQMLNPTAFIQS
                     TFNTIGAQIALLLKIHAYNVTYVHRGLSFESALTDGIMSIAEGKQHVLAGAMDEITPTS
                     YIIQQRLGLLKGTTAGEGAQFFLLSAQEEEQTFAELKGVDTFITRMSAPEISNRMHHFL
                     KSHELAPEDIDWFISGKNGQEATDAVYTELEHSLFPHALHSSFKEQCGEYQTASSYALW
                     MAAKALKEEASSRYALIYNQYQGINHSIILIKRCTS"
     CDS             complement(966..2156)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (128-392). E-value:
                     1.2e-36. Score: 118.4. Matches aSDomain:
                     nrpspksdomains_ctg584_2_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 264; E-value:
                     4.2e-80)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg584_2"
                     /sec_met_domain="APE_KS2 (E-value: 1.1e-142, bitscore:
                     465.5, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKIYVTGLGVISGIGQGVKENLDSLKAGKHGMGKITLFPTVLDV
                     PVSEVKQSNEELKQILSLPSGKTYSRTALLGLLAAQEAARDAELDFTSPRIGLISSTSI
                     GGMDASERFYASFREDNRKGRLRDIISHDCGASTEMIAAYLGVKGMVTTLSTACSSAAN
                     AIMLGARLIRHGYLDTVLVGGTDALCRFTLNGFNSLMILDKEHCRPFDRTRAGLNLGEG
                     AGYLVLQSEKSLTKAPYCELSGYANANEAYHQTGSSPDGNGPFLSMSQAIASAGLTAGA
                     IDYINVHGTGTPSNDASEGKAIRRIFDTRIPPFSSVKAFIGHTLGASEGIEAVYSVLSI
                     RHGLIYPNLNFHLSDEESGLIPETIFRSGLPIRHVLSNSFGFGGNNSSLVFSTLNR"
     aSDomain        complement(981..1772)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_2_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.20E-36"
                     /label="ctg584_2_PKS_KS.1"
                     /locus_tag="ctg584_2"
                     /protein_end="392"
                     /protein_start="128"
                     /score="118.4"
                     /tool="antismash"
                     /translation="IISHDCGASTEMIAAYLGVKGMVTTLSTACSSAANAIMLGARLIR
                     HGYLDTVLVGGTDALCRFTLNGFNSLMILDKEHCRPFDRTRAGLNLGEGAGYLVLQSEK
                     SLTKAPYCELSGYANANEAYHQTGSSPDGNGPFLSMSQAIASAGLTAGAIDYINVHGTG
                     TPSNDASEGKAIRRIFDTRIPPFSSVKAFIGHTLGASEGIEAVYSVLSIRHGLIYPNLN
                     FHLSDEESGLIPETIFRSGLPIRHVLSNSFGFGGNNSSLVFS"
     CDS             complement(2153..2410)
                     /NRPS_PKS="Domain: PP-binding (16-79). E-value: 1e-10.
                     Score: 33.9. Matches aSDomain:
                     nrpspksdomains_ctg584_3_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg584_3"
                     /sec_met_domain="PP-binding (E-value: 2.5e-11, bitscore:
                     33.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEELIEKLKVELIEALNLEEITPEDIDSEAPLFGDDGLGLDSIDA
                     LEIILILERNYGIKIENPAKGKEIFYSVRTLADYITAHQK"
     aSDomain        complement(2174..2362)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_3_PP-binding.1"
                     /evalue="1.00E-10"
                     /label="ctg584_3_PP-binding.1"
                     /locus_tag="ctg584_3"
                     /protein_end="79"
                     /protein_start="16"
                     /score="33.9"
                     /tool="antismash"
                     /translation="NLEEITPEDIDSEAPLFGDDGLGLDSIDALEIILILERNYGIKIE
                     NPAKGKEIFYSVRTLADY"
     CDS             complement(2416..4146)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (127-378). E-value:
                     5.9e-24. Score: 76.5. Matches aSDomain:
                     nrpspksdomains_ctg584_4_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 162.5; E-value:
                     2.7e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg584_4"
                     /sec_met_domain="APE_KS2 (E-value: 9.2e-125, bitscore:
                     406.5, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEHHLTTYITHDTLISALGFGTQENLEAIRGYHSGITLQTDKRIA
                     DTPLLAATISQERLQQQAETAGISAYPRMEQLFILTINELIRQSGQTLEDKTCGLILST
                     TKGNIDLLARHTEHPDEAVFLWKMAENIAGYFHAEERVHVISNACISGVSALVTGKRMI
                     ENGIYRKVIVVGGDLLSHFITSGFLSFRSLSSRPCRPYDSNRDGLNLGEACGAVLLSTE
                     KTPNSIILSGGAISNDANHISGPSRTGDGLFFAIRQAMQEAGTALQNISFVNAHGTATV
                     YNDEMESKALTLAHLEQVPTHSLKPYFGHTLGASGIIESIVCMHELKQGILFGTPGYET
                     PGVPMPIPVYATHQHIPMKHCVKTASGFGGCNAAIVLSLPEYAPFKDEDNTLPEIRCTR
                     EVRIENSSVFINNELIFHSEEPDFGIFIRDTYKKSGGNNMKFYKMDDLCKLGYVAAEYL
                     LKDKTFAPLEMGMLLANATSSLHTDIRHQQLIDQDGDRAASPAVFVYTLPNVVSGEICI
                     RHKIQGENTFFITKAYQPEKLERYARIVMQKGKLNYCIIGWCELLKNTYKAVFKLIEKQ
                     "
     aSDomain        complement(3013..3765)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_4_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="5.90E-24"
                     /label="ctg584_4_PKS_KS.1"
                     /locus_tag="ctg584_4"
                     /protein_end="378"
                     /protein_start="127"
                     /score="76.5"
                     /tool="antismash"
                     /translation="MAENIAGYFHAEERVHVISNACISGVSALVTGKRMIENGIYRKVI
                     VVGGDLLSHFITSGFLSFRSLSSRPCRPYDSNRDGLNLGEACGAVLLSTEKTPNSIILS
                     GGAISNDANHISGPSRTGDGLFFAIRQAMQEAGTALQNISFVNAHGTATVYNDEMESKA
                     LTLAHLEQVPTHSLKPYFGHTLGASGIIESIVCMHELKQGILFGTPGYETPGVPMPIPV
                     YATHQHIPMKHCVKTASGFGGCNAAIVLS"
     CDS             complement(4131..4574)
                     /locus_tag="ctg584_5"
                     /transl_table=11
                     /translation="MKRKTSPQQAALTDRTTIKVRFSEIDSMQIVWHGEYVRYFEDGRE
                     AFGKRYGLDYMSIYREGYVVPIVDLSCQFKQSLSFGEEAIVETRYIHSDAAKILFEYTI
                     YRASDQNIVATGTTTQVFLNTNRELELVNPAFYIEWKKKWNII"
     CDS             complement(4571..5008)
                     /locus_tag="ctg584_6"
                     /transl_table=11
                     /translation="MNRKEIICRGDELFRLIPQRPPIVMIDRFYGIEENTSWSGLTVTP
                     DNLFCRDGVLQETGIIEHIAQSAAARVGYIYMLREEPVPLGFIGSVEKMKIFRLPSAGA
                     ELYTGITIVQEIFDITLITAEVKENDELLAECRMKIYLKKE"
     CDS             complement(5005..6075)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 113.6; E-value:
                     1.9e-34)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg584_7"
                     /transl_table=11
                     /translation="MMHKRYTKEQLTAVEAQRLAQEIAFGPVVFQVSRLMLKFGIFQLL
                     ADKRKGYTLQEISVQTGLTRYAAQILLEASLTIGTILLEEDRYVLAKAGWFLLNDKMAR
                     VNMEFNHDVNYQGLFYLEEALLNGQPEGLKVFGGWPTIYEGLSQLPEQVQKSWFGFDHF
                     YSDNSFSQALEIVFLHSPKRLLDIGGNTGRWATRCVQYNQEVEVTIVDLPQQLEMMRKQ
                     TADVPGAERIHGHGANLLDRTVPFPSGFDTVWMSQFLDCFSEEEATSILSRVAQSASKD
                     SKVYIMETLWDRQPYETGSYCLTQISLYFTAMANGNSKMFHSDDLIRCIKNAGLEVEEI
                     HDNIGLGHSILQCRLK"
     CDS             complement(6091..6975)
                     /locus_tag="ctg584_8"
                     /transl_table=11
                     /translation="MPETWKGKTRGGTFGYMFFIYMIRCLGITAAYGFLSLVVLYFIPF
                     APKATASTWQYARNRLKYGRAKAAGLLLKNYYRLGQILIDKVAIGNGMADKYHFEFENY
                     QAFLNVLNGNTGVIMIGAHVGNWEIGAPFFDEYGKKINIVMFDAEHERIKEILEKNAST
                     RDYKIIPVNEDNLTHVFRITEALERKEYVCFQGDRYLNKEKLLSSPFMGKEAEFPMGPF
                     LLASKMRVPVVFYFAMREAKKNYRFHFFIAEAVVHTKEKRAEQALLEQYTQTLEKIVRQ
                     YPEQWFNYYPFWA"
     CDS             complement(6975..7226)
                     /NRPS_PKS="Domain: PP-binding (7-74). E-value: 4.6e-13.
                     Score: 41.4. Matches aSDomain:
                     nrpspksdomains_ctg584_9_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg584_9"
                     /sec_met_domain="PP-binding (E-value: 1.1e-13, bitscore:
                     41.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNEEIIEKIRTTLAEEFEIDVELIQPDAPLMQTLELDSLDLVDM
                     VVLIDRNFGFTVTAKDFAGIRTFQDFYNLVISHIQKED"
     aSDomain        complement(7005..7205)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_9_PP-binding.1"
                     /evalue="4.60E-13"
                     /label="ctg584_9_PP-binding.1"
                     /locus_tag="ctg584_9"
                     /protein_end="74"
                     /protein_start="7"
                     /score="41.4"
                     /tool="antismash"
                     /translation="EKIRTTLAEEFEIDVELIQPDAPLMQTLELDSLDLVDMVVLIDRN
                     FGFTVTAKDFAGIRTFQDFYNL"
     CDS             complement(7248..8477)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (77-405). E-value:
                     8.2e-50. Score: 161.7. Matches aSDomain:
                     nrpspksdomains_ctg584_10_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 308.8; E-value:
                     1e-93)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg584_10"
                     /sec_met_domain="APE_KS1 (E-value: 2.3e-167, bitscore:
                     546.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVVITGMGIYSCIGRNQEEVKESLFQGKSGIGIAPARKEMGYF
                     SALTGLVERPDLKKLLDRKKRHSLAEQGEYAYMATLEAFRQARIEEKFLLENEVGILYG
                     NDSSAAPVIQAIDIIREKKNTALVGSGSIFQSMNSTITMNLSVIFHLKGINFTISGACA
                     SGSHAIGMAYLLIKSGLQDCILCGGAQEVNPYSVGSFDGLGAFSAREDEPEKASRPFDK
                     NRDGLVPSGGGASLVLESYESAVKRGATILAEVIGYGFSSNGDHISVPNVDGPRRSLQM
                     AVKDAGIPLEEIAYINAHATSTPVGDLNEAKAIAEVFGSHHPYVTSTKSQTGHEMWMAG
                     ASEVIYSTLMMNNGFIAPNLNFEEPDEASALLNIPSRRVETEFDTFLSNSFGFGGTNSS
                     LIIRKFKENN"
     aSDomain        complement(7263..8246)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_10_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="8.20E-50"
                     /label="ctg584_10_PKS_KS.1"
                     /locus_tag="ctg584_10"
                     /protein_end="405"
                     /protein_start="77"
                     /score="161.7"
                     /tool="antismash"
                     /translation="MATLEAFRQARIEEKFLLENEVGILYGNDSSAAPVIQAIDIIREK
                     KNTALVGSGSIFQSMNSTITMNLSVIFHLKGINFTISGACASGSHAIGMAYLLIKSGLQ
                     DCILCGGAQEVNPYSVGSFDGLGAFSAREDEPEKASRPFDKNRDGLVPSGGGASLVLES
                     YESAVKRGATILAEVIGYGFSSNGDHISVPNVDGPRRSLQMAVKDAGIPLEEIAYINAH
                     ATSTPVGDLNEAKAIAEVFGSHHPYVTSTKSQTGHEMWMAGASEVIYSTLMMNNGFIAP
                     NLNFEEPDEASALLNIPSRRVETEFDTFLSNSFGFGGTNSSLIIRKF"
     CDS             complement(8485..9213)
                     /NRPS_PKS="Domain: PKS_KR (3-170). E-value: 1.2e-18. Score:
                     59.5. Matches aSDomain: nrpspksdomains_ctg584_11_PKS_KR.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     220.5; E-value: 4.2e-67)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg584_11"
                     /sec_met_domain="adh_short (E-value: 2.7e-37, bitscore:
                     118.4, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYALVTGGSRGIGRAICLKLAEMGYHILINYQSNDTEAEATLQQ
                     IREQGSDGELMKFDVTDAAATHAALNGWMEQHPDTYIEVLVNNAGIRRDNLMLWMEQEE
                     WSRVLDISLNGFFNVTQPLLKNMLVKRFGRIINIVSLSGIKGMPGQTNYSAAKGGIIAA
                     TKALAQEVAKKHVTVNAIAPGFIRTDMTDGIDENEWKKHIPVGRFGEPGEVAALVGFLA
                     SGQASYITGEVISINGGLYT"
     aSDomain        complement(8704..9204)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg584_11_PKS_KR.1"
                     /evalue="1.20E-18"
                     /label="ctg584_11_PKS_KR.1"
                     /locus_tag="ctg584_11"
                     /protein_end="170"
                     /protein_start="3"
                     /score="59.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ALVTGGSRGIGRAICLKLAEMGYHILINYQSNDTEAEATLQQIRE
                     QGSDGELMKFDVTDAAATHAALNGWMEQHPDTYIEVLVNNAGIRRDNLMLWMEQEEWSR
                     VLDISLNGFFNVTQPLLKNMLVKRFGRIINIVSLSGIKGMPGQTNYSAAKGGIIAATKA
                     LAQE"
     CDS_motif       complement(9139..9201)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg584_11_0001"
                     /evalue="2.30E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg584_11"
                     /protein_end="25"
                     /protein_start="4"
                     /score="20.2"
                     /tool="antismash"
                     /translation="LVTGGSRGIGRAICLKLAEMG"
     CDS             complement(9210..10742)
                     /locus_tag="ctg584_12"
                     /transl_table=11
                     /translation="MLAENKITLASLYKILFEEEPLTLSDPCMQKLEESFNFLKTFSND
                     KIIYGINTGFGPMAQYRIEDESLSQLQYNIIRSHATGAGQPLPELYVKAAMIARLYTFL
                     QGKSGVHKELALLITEFINRGIIPYVPEHGSVGASGDLVQLAHIALTLIGEGEVFYQGK
                     KRDTASVLAENGLQPFQMHIREGLSVTNGTSVMTGIGIVNLIYARQLMQWAVGASVMMN
                     EIAASYDDFMSEPLNGAKQHEGQQEIARMMRQWVKGSLCVRKRENELYNGKHEEKIFTH
                     KVQPYYSLRCIPQILGPVYDELLNAEKVLINEINSACDNPIVDPDTQNVYHGGNFHGDY
                     VSFEMDKLKIAVTKLTMLAERQINYLFHDRINGILPPFVNMGVLGLNYGLQASQFTATS
                     TTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALMAKTVIENAYQVMAILMMGIVQAI
                     DCLDIADRISPRSQQVYKEIRAFFPVFREDTPKYKEIESMMVYLKKGRFQEK"
     CDS             complement(10971..12452)
                     /locus_tag="ctg584_13"
                     /transl_table=11
                     /translation="MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHAR
                     PAITFDILFRYLTHLGYKVRYVRNITDVGHLEHDADEGEDKIAKKARLEQLEPMEVVQY
                     YLNRYHKAMEALNVLPPSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAKYNK
                     DHHYGKLSGRNLDDVLNTTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGW
                     HCECTAMGKKYLGEHFDIHGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITING
                     QKMGKSYGNFINLDEFFHGTHKLLTQAYSPMTIRFFILQAHYRSTVDFSNEALQAAEKG
                     LERLTEAVKGLERITPSAQTTGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINT
                     VLDKKATLSAEDLEELKSVFHLFMYEILGIKEEAANNEAREEAYGKVVDMLLEQRMKAK
                     ANKDWATSDKIRDELAALGFEVKDTKDGFTWRLNK"
     CDS             complement(12843..13007)
                     /locus_tag="ctg584_14"
                     /transl_table=11
                     /translation="MSMWSYDAGQQILTVNGVELYLQRETDWEANPRTHTIVYAGYANN
                     KTYWGKKSK"
ORIGIN
        1 tacagaaact aggatgtgca tcgcttgatt aagatgattg aatgattgat accttgatat
       61 tggttataaa tcaatgcgta acgggatgaa gcttcttctt tcaaggcttt tgccgccatc
      121 cagagagcat aggatgaggc agtctgatat tctccgcatt gctctttaaa gctgctatgc
      181 agggcatgag gaaacagact gtgttccagc tctgtataaa cggcatctgt tgcttcctgc
      241 ccgttcttgc cactgataaa ccagtcaatg tcttcgggtg caagctcgtg agattttaga
      301 aaatgatgca tcctgttact gatttccgga gcactcatcc gggtgataaa agtgtccacc
      361 cctttcagtt cagcaaaagt ctgttcctct tcttgtgcgc ttaacaggaa aaattgtgct
      421 ccctcaccgg ctgtcgttcc tttcaacagt cctaaacgct gctggatgat gtagctggta
      481 ggggttattt catccatagc acctgccagt acatgctgtt tgccttcggc aatactcatg
      541 atgccatctg tcagagcact ttcaaaactt aaaccacggt gtacatacgt cacattataa
      601 gcatgtattt tgagtagcaa agctatctgt gcaccgatcg tattaaaggt ggactgaatg
      661 aaagcggtgg gattgagcat ctgttcccgg ttatccatca aggcgttcat aaacttttcc
      721 gtatcagcca gacaacccag tcccgtagcg gtgataatgg catctacctt ttccggcgat
      781 atatctttca gacattccag tccgcaagct actcccatct tgatcatgcg gctcatgcgc
      841 ctccgcagat tggcgttggt gatcatttct ttataatcag gttcctttgc ggagaaataa
      901 ggcttttcat tgctgctctc tgcatgaata gaggcgatac gattgatata tactgggcac
      961 atacgctatc tgtttagagt ggaaaatacg agagatgagt tgtttccgcc gaatccgaag
     1021 gagttggata atacatgccg tatgggaagt cccgaccgga aaatcgtttc aggaatgaga
     1081 ccgctttctt catcggacag atggaagttc agattagggt aaatcagtcc gtgacggatg
     1141 gagagtacgg aatatacggc ttctattcct tccgaggctc ccagggtgtg accgatgaaa
     1201 gccttgaccg aactgaaagg tggaatacgg gtatcgaaga tacgccggat ggctttccct
     1261 tcggaagcgt cgttactggg agtacctgtg ccatgtacat tgatatagtc tatggctccg
     1321 gctgtcagtc cggcagaggc tatggcctga ctcatggaaa ggaaaggccc gtttccatcg
     1381 ggggaacttc ctgtctgatg gtaggcttcg ttggcattgg cgtaaccgct gagttcacaa
     1441 taaggagcct ttgtaaggga tttctccgat tgaagaacaa ggtatccggc tccttctccc
     1501 aggtttaatc cggcacgtgt acggtcgaaa ggacggcagt gctctttgtc cagtatcatc
     1561 aatgaattga aaccgttgag cgtgaaacgg cataaggcat ccgttccacc tacgaggacg
     1621 gtgtccagat aaccgtgtcg aatcaggcgt gcccctaaca taatggcatt tgcagccgaa
     1681 gaacaggcgg tgctgagggt ggtcaccatt cctttcactc ctaaataagc ggctatcatt
     1741 tcggtactgg ctccacagtc atgggagata atatcacgta atcttccttt tcggttatcc
     1801 tcccggaaag aggcataaaa ccgctcgctt gcatccattc ctccgatgga agtggaagaa
     1861 atgagtccga tacgggggga agtgaaatcg agttccgcat cacgggccgc ttcttgagct
     1921 gccaacaggc ctagcagggc tgtgcgtgaa tacgttttgc cggatggaag ggacaggatt
     1981 tgctttaatt cttcattgct ctgtttcact tcggacacgg gtacgtcaag cacggtgggg
     2041 aaaagggtga ttttccccat accgtgtttt ccggctttta aggagtccag attttctttt
     2101 actccttgtc ctatgcccga aatcactccc agaccggtga catagatctt cctcatttct
     2161 gatgggcggt gatgtagtct gccagggtac gcaccgaata gaatatttcc tttcctttgg
     2221 caggattctc tattttaata ccgtaattcc tttccagaat caggataatc tctaatgcgt
     2281 caatcgagtc aagacccaat ccgtcgtctc cgaataaagg tgcttcacta tcaatgtcct
     2341 ccggagtgat ttcttccaga ttcaatgctt ctattaattc aactttcaat ttctcaatca
     2401 attcttccat attctttatt gtttttcaat cagtttaaat actgctttat aggtgttttt
     2461 caataattca caccatccga tgatacaata gttcagtttc cctttttgca tgactatacg
     2521 tgcgtatctt tccagtttct ccggctgata ggctttagtt atgaaaaagg tgttttcccc
     2581 ttgtatcttg tggcggatgc aaatctcgcc ggacactaca ttgggtagtg tgtaaacaaa
     2641 tacagccgga ctggctgccc ggtctccgtc ctggtctatc agttgctggt gtctgatatc
     2701 cgtatgtagg gatgaagtgg cgttggcaag taacatcccc atttccagtg gggcgaatgt
     2761 cttgtctttc agtaagtatt cggcggcaac atatcccagt ttgcaaaggt catccatttt
     2821 atagaatttc atattgtttc cgcctgattt tttataggta tcacgtataa atatgccgaa
     2881 atccggttct tcagaatgaa aaataagttc gttgtttatg aagacagaac tgttttcaat
     2941 acgaacctcc cgtgtacatc ggatttcggg caaggtgttg tcctcatcct tgaacggagc
     3001 atattccggt aaagacaaga caatggctgc gttacaccca ccgaatcccg aagcggtttt
     3061 tacacaatgt ttcatcggaa tgtgttggtg agttgcatag accggtatag gcataggcac
     3121 acccggtgtt tcgtaacccg gagtgccgaa caggatgcct tgtttcagtt cgtgcataca
     3181 gacaatgctt tcaatgattc ccgatgctcc caaggtatgc ccgaaatagg gttttaagct
     3241 atgtgtggga acttgttcca gatgagccag cgtgagggct ttggattcca tttcgtcatt
     3301 gtatacggtg gccgtgccat gggcattgac aaagctgatg ttttggaggg ctgttcctgc
     3361 ctcctgcatg gcttggcgga tagcgaaaaa gagtccgtct cctgtacggg aagggccgga
     3421 gatatggttg gcgtcattac ttatggctcc tccggagaga atgatgctat ttggcgtctt
     3481 ttccgtactt agtaatacgg ctccgcaagc ctctcccaag ttcagtccgt cacggttgct
     3541 gtcatacgga cggcatggac gggagctgag ggaccggaaa gacaggaaac cactggtgat
     3601 gaaatgagag agaaggtcac ctcccactac aattactttg cggtagatgc cgttctctat
     3661 catgcgtttt cctgttacaa gggctgacac tcctgatatg caggcgttgg agatgacatg
     3721 tacccgttcc tcagcatgga aataaccggc aatgttttct gccattttcc aaagaaagac
     3781 cgcttcatcg ggatgttccg tatgacgggc cagtaagtcg atgttccctt tggtggtgga
     3841 gaggatgagt ccgcaggttt tgtcttccaa agtttgtccg gactgacgga tgagttcgtt
     3901 gatagtcagt atgaacagtt gttccatccg gggatatgcg cttattcctg ctgtttctgc
     3961 ctgttgttgc aatctctctt gcgagatagt agctgccaat aggggagtgt ccgcaatccg
     4021 tttgtccgtc tgcagggtga taccgctgtg atagccccgg atggcttcca gattttcctg
     4081 tgtgccgaaa cccagcgcgc tgatcagggt atcatgggtg atataggtag tcagatgatg
     4141 ttccatttct ttttccactc tatgtaaaag gcaggattaa ccaattccag ttctctattt
     4201 gtattcagga atacctgtgt agtggttcct gttgccacaa tgttttggtc tgatgcccgg
     4261 tagatggtgt attcaaaaag gattttggca gcatcactat gaatataccg tgtctcgaca
     4321 atggcttcct caccgaatga cagcgactgt ttgaactggc agctcaggtc tacgatagga
     4381 actacatagc cttcccggta gatgctcata tagtccaagc catatcgctt tccgaaagct
     4441 tcacgtccat cttcgaaata acgcacatat tcaccatgcc atactatctg catggaatct
     4501 atttcactga agcggacctt gatggtggtc cggtctgtca gtgctgcctg ctgtggactt
     4561 gtttttcgtt tcattctttt ttcagatata ttttcatccg gcattcggcc agaagttcat
     4621 cgttttcttt cacctcggcg gtaatcagtg tgatatcgaa tatttcctgc acaatggtta
     4681 ttccggtata taattctgct ccggcagaag gaagacggaa tattttcatt ttttctacag
     4741 agccgataaa gcctagtggt acaggctctt ctcgcagcat atagatatat cctacccgtg
     4801 ccgctgctga ttgggcgatg tgttctatga ttcctgtttc ctgtaatacg ccgtcccggc
     4861 agaacagatt atcgggggtg acggtcagcc ctgaccagga tgtattttct tctatcccat
     4921 agaaacggtc tatcatgact atgggcggac gttgtggaat cagccggaac agttcgtctc
     4981 cccgacaaat gatttctttt ctgttcattt cagtctgcat tgcaggatac tgtgtcccag
     5041 tcctatgtta tcatgaatct cttccacttc cagtccggca tttttaatac agcggatcag
     5101 gtcatccgaa tggaacatct tgctgttgcc gttggccatg gctgtgaagt aaaggctgat
     5161 ttgagtcagg cagtaagagc ccgtttcgta tggctgccgg tcccacagcg tttccatgat
     5221 gtagacttta ctgtctttgc tggcggactg tgccacacga cttagaatac tggttgcttc
     5281 ctcttccgaa aagcagtcca gaaactggct catccatacg gtgtcaaagc ctgaaggaaa
     5341 gggcacggtc cgatccagca gattggcgcc gtggccatgg atgcgttctg caccgggcac
     5401 gtcggcagtt tgttttctca tcatttccag ttgttgaggc aaatccacaa tggttacttc
     5461 tacttcttga ttatattgga cgcatcgggt tgcccatctg ccggtgtttc caccaatatc
     5521 gagcagtcgt ttcggtgagt gaagaaatac aatttctaat gcctggctga acgagttgtc
     5581 cgagtagaaa tggtcaaagc caaaccagct tttctgtacc tgttccggca gttgggaaag
     5641 tccttcgtaa atggtaggcc atccgccgaa tactttcaat ccttccggct gaccatttaa
     5701 aagagcctct tccagataga aaagtccttg atagttcaca tcgtgattga attccatatt
     5761 gacacgtgcc attttatcat tcagcagaaa ccagcctgcc ttggccagta catagcggtc
     5821 ttcttccaac aggatggttc cgatggtcag cgatgcttcc agtaatattt gtgctgcata
     5881 gcgtgtcagt ccggtctgca cactgatttc ttgcagcgtg tatcctttcc gtttgtcggc
     5941 cagcaattgg aatattccga atttcaacat cagacgtgaa acttggaata ccacgggacc
     6001 gaaagcgatc tcttgcgcca gtctttgtgc ctctacagct gtcaattgct cttttgtata
     6061 tcttttatgc atcataatcc agatttgtat ttatgcccag aacgggtagt aattgaacca
     6121 ttgttccgga tactgccgga cgattttttc cagcgtttgt gtatattgct ccaataatgc
     6181 ttgttcggcc cgcttctctt tggtatgtac caccgcctcg gcaataaaga aatggaaacg
     6241 atagtttttt ttcgcttcac gcatggcaaa atagaatacg acaggtactc tcattttgga
     6301 agccagcagg aaaggaccca tcggaaattc ggcttctttt cccatgaacg gactgctgag
     6361 taatttctct ttattcagat agcggtcacc ttgaaagcag acatattctt tgcgctccag
     6421 tgcctctgta atacggaata catgggtcag attgtcttca tttacgggaa ttattttata
     6481 atcgcgtgtg gaggcattct tttccagaat ctcttttatc cgttcgtgtt cggcatcaaa
     6541 catgacaata ttgatttttt tgccgtattc atcgaagaaa ggagcaccta tttcccaatt
     6601 acctacatgg gcgcctatca taattacgcc ggtgtttcca ttcagtacgt tcaaaaatgc
     6661 ctgatagttc tcgaactcga aatgatattt gtctgccatc ccattgccga tggctacttt
     6721 gtcaatcaat atctgcccca gccggtagta atttttcaat aacagtccgg cggctttggc
     6781 acgtccgtat ttcagtctgt tccgtgcgta ttgccaggta ctggcggtag ctttcggtgc
     6841 gaaaggtata aaatagagga cgaccaatga cagaaagccg taagccgcag tgatacccag
     6901 acagcgaatc atgtagatga agaacatgta tccgaatgtg cctcctcttg ttttaccttt
     6961 ccatgtttcg ggcatcagtc ttctttttgt atgtgcgaaa ttaccagatt atagaaatcc
     7021 tggaaggttc tgataccggc aaagtctttg gctgtcacgg tgaaaccgaa attcctgtca
     7081 atcagtacta ccatatctac caaatcaagg ctatccaatt ccaaggtttg catcaaaggg
     7141 gcgtccggct gaatcaattc tacatcaatc tcaaattctt cggccagggt ggttctgatc
     7201 ttttcgatga tttcttcgtt ggtcataaat ttatatattg gtttaaatta attgttttct
     7261 ttgaatttgc ggataatcag tgaggaattg gttcctccga atccgaatga gttggataag
     7321 aatgtgtcaa attccgtttc tacccttcgg gaagggatat tcagtagggc agaggcttca
     7381 tcgggctcct caaaattgag gttgggtgcg atgaatccgt tgttcatcat caaagtggag
     7441 taaataactt cactggctcc tgccatccac atctcatgtc cagtctgtga tttggtggat
     7501 gtgacgtagg gatgatggct cccgaatact tccgctatgg cctttgcctc gttcaaatca
     7561 cctacaggag tggatgtggc atgtgcattg atgtatgcaa tctcttccaa aggaataccc
     7621 gcatccttga ctgccatttg tagagagcgt ctggggccat ccacattggg tacggaaatg
     7681 tgatctccgt tggatgaaaa gccgtatcct atgacttctg ccaggatagt cgctccgcgc
     7741 tttacggctg actcataact ttccagcacc agactggcgc ctccgccgct aggtactagt
     7801 ccgtcacggt ttttatcgaa tggacgggat gctttttccg gttcgtcttc ccgggcggag
     7861 aaggctccca acccgtcgaa actacccacg gaataagggt tcacttcctg tgctcctccg
     7921 cacagaatgc agtcctgaag tcctgacttg ataagcagat aagccatgcc gatggcgtgt
     7981 gaaccactgg cacaggctcc cgatatagtg aagttaattc cttttagatg gaaaataacc
     8041 gaaagattca tggtaatggt ggaattcatg gactggaaaa tagaaccgga accaaccaat
     8101 gccgtattct ttttctcgcg gataatatct attgcttgaa taaccggagc agcactactg
     8161 tcattgccat acagtattcc gacttcgttc tccaacagga acttttcttc tatccgggct
     8221 tgccggaatg cttccagggt agccatatag gcgtattctc cctgctcggc aaggctatgg
     8281 cgtttcttgc ggtccaataa tttcttcaaa tcgggacgtt cgacaagacc ggttaacgcc
     8341 gagaaatagc ccatttcttt gcgagccgga gcgattccga tgcctgactt tccttgaaac
     8401 agtgactctt tcacttcctc ttgattcctg cctatgcacg aatagatgcc cattcctgta
     8461 ataactactc ttttcatctt ttgcttacgt atataatcct ccattgatgg aaatcacttc
     8521 tccggtaatg taggaagctt gcccggatgc caggaagcct accagtgcgg ctacttcccc
     8581 gggctcgccg aagcgtccta ccgggatgtg ttttttccac tcgttctcat caattccgtc
     8641 tgtcatatct gtacggataa atcccggtgc tatggcattt accgtcacgt gttttttggc
     8701 aacttcctgc gccaacgctt tggtagcggc tatgattcca cctttggctg ccgaatagtt
     8761 ggtctgcccc ggcatccctt tgatgcccga cagggaaacg atattgataa tacgtccgaa
     8821 tcgctttacc agcatattct tcagcaaggg ttgggtgacg ttaaagaaac cgttcaggct
     8881 gatatccagt acccggctcc attcttcctg ttccatccac agcatcaggt tgtcacggcg
     8941 tataccggca ttgttcacca gtacctcaat gtaagtatcg ggatgctgtt ccatccatcc
     9001 attcagtgcg gcgtgtgtgg ctgctgcatc tgtcacgtca aatttcatca gttcgccatc
     9061 cgagccttgt tcccgtattt gttgcagggt ggcttcagct tccgtatcgt tgctttggta
     9121 gtttatcagt atatgatatc ccatttcggc cagtttcaga caaatggctc gtccgattcc
     9181 ccggcttcct cctgttacta atgcgtattt catttttctt gaaacctccc ttttttaaga
     9241 tataccatca tgctttctat ctctttatat ttaggtgtat cttcccggaa aacagggaaa
     9301 aatgctcgta tttctttata tacttgttgt gaccgggggc ttatccggtc ggctatatcc
     9361 agacaatcaa tggcttgtac aatacccatc atcaggatag ccatcacctg ataagcattt
     9421 tcaatgactg tttttgccat caaggcggaa ttggtaccca tgcttacaat gtcctggttg
     9481 tcattgttgt tggggatgct atgcacatac atcggattgg acaaagtctg acattctgcc
     9541 gtggtagagg tggctgtgaa ctgggaggcc tgcaaaccat aattcagccc cagcactccc
     9601 atattgacaa agggggggag aatgccgttg atgcggtcgt gaaacaggta attaatctgc
     9661 cgttcggcaa gcatcgtcag tttggtgact gctattttca acttgtccat ttcaaaagat
     9721 acataatctc cgtggaagtt accaccatga tatacattct gtgtgtcagg gtccactatc
     9781 gggttgtcac aagctgaatt tatttcgttt atcaatactt tttcagcatt gagtaattca
     9841 tcataaaccg gccctagaat ttgtggaatg caacgtagcg aataataggg ctgcacttta
     9901 tgggtgaaga tcttctcttc gtgcttccca ttataaagtt cattctctct tttgcgtaca
     9961 cacaggctgc cttttaccca ttggcgcatc atgcgggcta tctcttgttg tccttcatgc
    10021 tgtttggccc cgttcagcgg ttcagacatg aagtcatcgt atgaggcggc tatttcattc
    10081 atcataacag atgcacctac cgcccactgc ataagttggc gtgcgtaaat cagattgaca
    10141 atacctatac ctgtcatgac tgaagtgccg ttggtcactg aaagcccttc gcggatgtgc
    10201 atttgaaaag gttgcaatcc gttttccgcc agcacagagg ccgtatctct tttcttgcct
    10261 tggtaaaaga cttctccttc tccaatcaga gtgagggcga tatgggcgag ttgtaccaga
    10321 tcaccactgg cccccacact gccgtgttcg ggtacgtatg gaataatccc ccggttgata
    10381 aactcggtaa tgagcagcgc cagttcttta tgtacgccgg attttccttg cagaaaagta
    10441 tagagacgcg ctatcatggc agctttgaca tagagttccg gaaggggttg ccctgctccg
    10501 gtagcatggc tgcggatgat attatactgt aattgtgaca aggattcatc ttcaatacgg
    10561 tattgggcca tcggtccaaa gccggtattg atgccataga tgatcttgtc gttggagaat
    10621 gtttttagaa aattgaaact ctcttccaat ttttgcatac atgggtcgga gagtgtcagt
    10681 ggctcttctt caaacaaaat cttatacagg gacgctaaag ttatcttatt ctcagctagc
    10741 attggattgg tgtgataagc agttttgtat aatttctgca aaaatagtta tttaaaaata
    10801 agtattacca tccgaaggga gatttttcag ttttgatggt tatctgaaag gttcaccaca
    10861 gattatacaa ataaacgcag atgcttctta aatcattgat tgttaggttg gataatctgt
    10921 gtgaatctgt atagtctgtg gtgaaaaaat atagtaccgc tctacgtatt ctatttattc
    10981 agtctccatg tgaaaccatc tttggtatct ttcacttcaa agccaagagc ggctagctca
    11041 tcacggattt tatcacttgt agcccagtct ttattggctt tggctttcat acgttgttcc
    11101 agcagcatat ctactacttt gccatatgcc tcttcacgtg cctcgttatt ggcggcttct
    11161 tcttttatac ccaagatttc atacataaag agatggaaca cacttttcag ctcttccaga
    11221 tcttcggcgg aaagggtcgc ttttttatca agcactgtgt tgatcatacg ggcgccgtca
    11281 aacagatggg cgatgacaat cggtgagttc aggtcatcgt tcatggctgt atagcatttt
    11341 tcacgtaagt cttttacgcc ttctatgccg gtggtttgtg cgcttggggt gatgcgttca
    11401 agacctttta cagcttctgt tagtcgttcc aatccttttt cggctgcttg taatgcttcg
    11461 ttactgaagt ccactgtact gcggtaatgt gcctgaagga tgaagaaacg gatggtcatg
    11521 gggctgtagg cttgtgtcag taacttgtga gtaccgtgaa agaactcatc caagttaata
    11581 aagttgccgt atgattttcc catcttctgc ccattaatgg taatcatgtt gttgtgcatc
    11641 caatagtgaa ccatgtcatc tccttgtgaa gccacgcttt gtgcgatctc acattcgtgg
    11701 tgtgggaaaa ttaagtccat tccccctcca tgaatatcga aatgctcgcc caaatatttt
    11761 ttgcccattg cggtacattc acaatgccaa ccgggaaaac cgttactcca cgggcttggc
    11821 cagcgcatga tatgttcggg ttgtgcacat ttccacagag cgaaatcggc aggattacgc
    11881 ttctcgcttt gaccgtccag ttcgcgggtg gtgttcagca catcgtccag gttacggccg
    11941 gacagtttgc cgtaatgatg gtctttgttg tattttgcta catcgaaata aacggaacct
    12001 tcactttcat aagcatagcc gtttttcaga atttcttcta ccagctctat ctgttcaatg
    12061 atatgacctg atgcatgtgg ctcgatactg ggtggaagta cattcaaggc ttccattgcc
    12121 ttgtggtagc gattgaggta atattgtact acttccatgg gttccaattg ttccaaacgg
    12181 gcctttttgg cgattttatc ttcaccttcg tctgcatcgt gttccagatg accgacatcg
    12241 gtaatgttac ggacataacg taccttgtat cccagatggg taagataacg gaacaggata
    12301 tcgaacgtga tggcgggacg tgcgtgtcct aaatgggcgt cgccatatac ggtaggaccg
    12361 catacataca tacctacatg aggggcatgc aagggaacga aaagttcctt ttgacggctt
    12421 aatgtattgt aaatggttag tttattttcc ataattcatt ctattagaat tgcaaagtta
    12481 taaattaaag tgtgtatttg gctaataatt aacaaaaaat ctgatgctgg tgtcctctgc
    12541 ttgtctacaa atgatgtttg ttggtttaag gaatagagat ttatggtgat aaataaaagt
    12601 aagaattagg ataaaaggtt gtctttgaac gtaaaagaac aaaataatga ttttttgtat
    12661 ggagattagg gaacctaact ctttttcctt ttcatgaaat aggtttctct tgtatctgtt
    12721 taatgatctt taatttctgc tattgacttg taaaacagga ccactgcccc ataaacattg
    12781 gtgttgtttt gaggggcagt ggtcctgttt ctatatgaag tttgcgtgcg ggagcgggaa
    12841 tgttatttgg atttctttcc ccaatacgtc ttgttgttgg catagccggc atagacgatg
    12901 gtatgggtac gtggattggc ttcccaatcg gtttcacgtt gcagataaag ttccactccg
    12961 ttaacagtca gaatctgttg gccggcgtca tagctccaca tactgcc
//
