LOCUS       c00300_gnlPro..         5929 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JIEFPDNF_300.
ACCESSION   c00300_gnlPro..
VERSION     c00300_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:17:25
            Original ID  :: gnl|Prokka|JIEFPDNF_300
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5929
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5929
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3428:5927]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3429..5927
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5929
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5929
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(27..434)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HBT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg300_1"
                     /sec_met_domain="HBT (E-value: 1e-09, bitscore: 27.7,
                     seeds: 132, tool: rule-based-clusters)"
                     /sec_met_domain="HBT_2 (E-value: 1.6e-10, bitscore: 30.5,
                     seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYIFKLTDKVRDYECDLQGVVNNSNYQHYMEHTRHEFLESLGE
                     NFGKMHEKGVDGFVSRVEIQYKTSLKSGDSYISCLNVYKKGVKLVFEQDIYRASDNALA
                     TKGIVESVIVENGKLTRGEYFDEMLKRIEHT"
     CDS             complement(434..1669)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_U32"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg300_2"
                     /sec_met_domain="Peptidase_U32 (E-value: 6.9e-70, bitscore:
                     223.7, seeds: 96, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPVGSYESLMAAIQGGADSIYFGIEGLNMRSRSSNNFTTDDLRK
                     IVALCREHGIKSYLTVNTVIYGEDLPLMREIIDAAKEAEVSAIIAADVAAMNYANSIGQ
                     EVHLSTQLNISNAEALKFYARFADVVVLARELNLKQVHEIYRQIVDQQITGPKGELIRI
                     EMFAHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYSVKDKDSNIELDIENQYIM
                     SPKDLKTIHFMNKMMDAGVRVFKIEGRARGPEYVRLVTECYKEAVRAYCNGTFDEKKVA
                     AWDERLRSVFNRGFWDGYYLGQRLGEWSSKYGSGATKKKVYVAKGIKYFDKIGVAEFEM
                     ESGNLKVGDEILITGPTTGAVMQTIEEIRVDLKPVDETVKGERFSLKVNEKIRPSDRLY
                     KMEKVQLEKVFE"
     CDS             1923..2063
                     /locus_tag="ctg300_3"
                     /transl_table=11
                     /translation="MISAYHFTYIIDSMTFVLFIDQLLHFLVDILLSLFLAGNQYLCPR
                     H"
     CDS             2074..3105
                     /locus_tag="ctg300_4"
                     /transl_table=11
                     /translation="MKIGTIDLGERPIFLAPMEDVTDISFRLMCKRFGADMVYTEFVSA
                     DALIRSVNKTQQKLNVADEERPVAIQIYGRDVPTMVEAAQICEAAHPDILDINFGCPVK
                     RVAGKGAGAGMLRNIPLMLDISREVVKAVNIPVTVKTRLGWDADNRIIVDLAEQLQDCG
                     IAALSIHGRTRAQMYTGEADWTLIGEVKNNPRIQIPIIGNGDVTSAEICKQRFEQYGVD
                     AVMIGRGSIGRPWIFREVRHYLDTGETLPAESFSWYLDILKKQVEQSVERLDERRGILH
                     IRRHLAATPLFKGIADFKQTRVAMLRAETVEALFDILDSIPDKFHTGYGFQSDSQVSES
                     IDC"
     CDS             complement(3078..3236)
                     /locus_tag="ctg300_5"
                     /transl_table=11
                     /translation="MKKTHCKYKVYLLNTQEFVITFYCKFATFCFKIDTVCGFTLLLYQ
                     QSIDSDT"
     CDS             complement(3429..4184)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_6"
                     /sec_met_domain="Fer2_3 (E-value: 5.3e-27, bitscore: 83.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.6e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.7e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKGPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANAIPISKADADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFISAKLQD"
     CDS             complement(4212..5927)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 155.8; E-value:
                     6e-48)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_7"
                     /sec_met_domain="FAD_binding_2 (E-value: 6.8e-49, bitscore:
                     155.8, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.9e-39,
                     bitscore: 123.5, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AQGGINAAKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAEVS
                     NSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRQIG
                     LGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERFAAHAVVVATGGYVNTFFLSTN
                     AMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKLTLMSESLRNDGRIWVPK
                     KLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNS
                     TGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDDPYETPMMIYPALHYSMGGLW
                     VDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQVP
                     RFSTDLPEFVEAEKAINARIAKLMNVKGKETVDSIHRKLGHIMWEHVGMARDAEGLKEA
                     IQMLKDLKKEFWSNVFIPGEANSLNVELDKALRLADFIEIGALMAHDALDRAESCGGHF
                     RTEHQTEEGEAMRHDDKFMYVSCWEYQGEDKDPVMRKEDLNYQFVVPQTRNYKK"
ORIGIN
        1 gccgatttgg taaatccgtt tgtcagtcat gtatgttcta ttctttttag catttcgtcg
       61 aaatattcac cgcgtgtcaa ctttccgttc tcaacgataa ccgactcgac gattcctttc
      121 gtcgccaatg cattgtcaga agccctatat atatcctgct cgaatacgag cttgactcct
      181 tttttataga cattgaggca agagatatag ctgtcgccgc tcttgagcga ggtcttatac
      241 tggatttcta cccgcgatac aaatccgtca accccttttt cgtgcatctt tccgaagttc
      301 tcgccaagcg actccaagaa ttcatgccga gtatgttcca tataatgctg ataattggag
      361 ttgttgacca ctccttgcag gtcgcattcg tagtcacgga ccttgtctgt cagtttaaaa
      421 atatactctt tcattattca aatacctttt caagttgaac cttttccatt ttatacaatc
      481 ggtcagacgg ccgaatcttc tcgttcacct tgagagagaa ccgttcacct ttaacggtct
      541 cgtccaccgg cttcaagtcc acacggattt cttctatcgt ctgcataacc gcacccgttg
      601 tcggtccggt aatcaggatc tcatcaccca ctttcaggtt accggattcc atttcgaact
      661 ccgccacgcc gatcttgtcg aaatatttga ttcctttggc aacatacact ttcttcttag
      721 tcgcgccgga gccatatttg ctactccact cacccaaccg ttgccccaaa taatagccgt
      781 cccagaatcc tcggttgaac acactgcgca ggcgttcgtc ccaagcggca actttctttt
      841 catcgaacgt gccattgcaa taagcacgca cagcttcctt atagcattcg gtcaccaggc
      901 ggacatattc gggaccgcgg gcacgtcctt cgatcttgaa tacccgtaca cctgcatcca
      961 tcatcttatt catgaaatga attgtcttaa ggtctttagg cgacataata tactggtttt
     1021 cgatatccag ttcgatattg ctgtctttat cttttaccga ataagcacgc cgacaaatct
     1081 gcatgcaagc tccccggttg gcggaagcgt tcatttcgtg caggctcagg taacatttac
     1141 cggaaacggc catacagaga gctccgtggg caaacatttc gatacggatc agttctcctt
     1201 tcggtcccgt aatctgctgg tcgacgatct gccgatagat ttcatgcacc tgtttcaaat
     1261 tcagctcacg cgccaagacg acaacatcgg caaaacgggc atagaatttc agtgcttccg
     1321 cattggaaat gttgagctgt gtggaaagat gcacctcctg tccgatgctg ttggcatagt
     1381 tcatagctgc cacatcggcc gcaatgatcg ctgaaacttc cgcctccttc gccgcatcga
     1441 taatctcgcg catcagaggc aggtcttccc cataaataac cgtattgacg gtcaaatagc
     1501 tcttaatacc gtgctcacga caaagcgcaa cgatcttgcg caaatcgtct gtcgtaaagt
     1561 tattggaaga gcgcgaacgc atattcaatc cttcgatacc gaaatagata gaatcggctc
     1621 ccccttggat agccgccatc agtgattcgt acgaaccgac aggcgccatt atttcaaaat
     1681 ctttctcgtt caagttctta tacatttact ggttcgttcg caaaggtagg aataatatcc
     1741 gagaagaatt gcatgggtat ccgtttctca gagcatcaaa atcgtgatca aataccattt
     1801 tctataaaga tttgcaaaac aaccatcata ctgtcattgt tgtcctttag aacctgttat
     1861 tcaatacctt aagacatgat agtttctatt tttaactatc atactactat catgtaacta
     1921 tcatgatttc agcttatcat tttacctata tcattgacag tatgacattt gtacttttta
     1981 ttgatcaatt actacatttt ttagtagata tccttttatc cctgttttta gcaggaaatc
     2041 agtacctttg tccccgtcat tgataatata aatatgaaaa taggtacaat agatttaggc
     2101 gaacgcccca tcttcttagc tccgatggaa gacgttacag atatttcttt ccggttgatg
     2161 tgcaaacgtt ttggtgccga tatggtttat accgaattcg tttcagcaga cgccctgatc
     2221 cgcagtgtaa ataaaacaca gcaaaagctg aatgtggcgg atgaagaacg tccggtcgcc
     2281 atacaaatat atggccgtga tgttccgaca atggtcgaag ccgcgcaaat ttgcgaagcc
     2341 gctcatccgg acattctcga tatcaatttc ggttgccctg tcaaacgagt ggcaggcaaa
     2401 ggagcaggcg ccggcatgct gcgcaatatt ccactgatgc tggacatcag ccgtgaagtc
     2461 gtcaaagcgg tgaacatccc tgtcaccgta aaaacccgct tgggctggga tgccgacaac
     2521 cggatcatcg tagacttggc cgaacagcta caggattgcg gtatcgcagc cctttccatc
     2581 cacggacgta cccgggcaca gatgtacaca ggagaggcag actggacact gatcggtgaa
     2641 gtaaaaaaca atccccgcat acaaattccc atcatcggca atggcgatgt gacttccgcc
     2701 gaaatctgca aacaacgttt cgaacaatat ggcgtggatg ccgttatgat tggccggggc
     2761 agcataggcc gcccctggat tttcagagaa gtgaggcatt atctggatac aggcgagaca
     2821 ttgcctgccg aatctttttc atggtatctg gacatactga aaaagcaggt ggaacaaagc
     2881 gtggaacgtt tggatgagcg ccggggaatt ctccacattc gccgccattt ggctgcaact
     2941 ccgctgttta aaggtattgc agacttcaag cagacacgcg tcgccatgct acgtgcggaa
     3001 acagtggaag cattattcga cattctggat tccatccccg acaaatttca tacaggatac
     3061 ggatttcaaa gtgactctca agtgtcagaa tcgatcgatt gttgatataa taacagtgtg
     3121 aatccgcaca cagtgtcaat tttaaaacaa aaagtagcaa atttgcaata aaatgtaatt
     3181 acaaactctt gcgtattcag taaatatact ttatatttgc agtgagtttt tttcatagta
     3241 ttttagatct aaggttaaca aagtctttgc agagagggaa tcgagagact gtttcactgg
     3301 aaagcaacaa aaaagccgca tccactttta ttatagggag tgcggctttt ttgtgggttt
     3361 gaacgaacat aaagaaagcc cgttgccatt ggcgacgagc ttttccatat acatatatat
     3421 ataatgtatt agtcttgtaa cttagcagag atgaactcgc ggttcaagcg agcgatgttg
     3481 ctaacggaga tgttcttcgg acattccatt tcgcaagcac gagtgttcgt acagttacca
     3541 aagcccagtt catccatctt agcaaccatt gcttttgcac ggcgagcagc ttccacctta
     3601 ccctgaggca aaagagccag ctggctaacc tttgcagaga cgaacagcat agctgaaccg
     3661 ttcttacaag cagcagcaca agcaccgcag ccgatacaag cagcagcatc catagccaaa
     3721 tccgcatcag ccttagaaat aggaattgca ttagcatcag gaaccccacc tgtgtttaca
     3781 gaaacgaaac cacctgcctg gataatctta tcataagcat aacggtctac catcaagtcg
     3841 cggattaccg ggaaaccggc agaacgccac ggctcgatcg taatcgtctc accatcatta
     3901 aaacggcgca tatagagctg acaggttgtt gcacctgttg ccggtccgtg cggatgtccg
     3961 tttacataca atgaacacat accgcagata ccttcacggc agtcgtggtc gaatacgata
     4021 ggttctttgc cttcgctaac caaacgttca ttcaaaatgt ctaacatttc caagaaagaa
     4081 acactctgat tgattttgtc gatttggtaa gtctcaaact gtcctttatc tttcggacct
     4141 ttctgacgcc aaactttaag tgttaccttt atatctttat ccatgattct aatttttgtg
     4201 attgatgatt attacttctt gtagttacgt gtttgaggaa caacgaactg atagttcaaa
     4261 tcttccttcc gcataaccgg gtctttgtct tcaccctggt attcccaaca agagacgtac
     4321 atgaacttat cgtcatgacg catagcttcg ccttcttcgg tctgatgttc cgtacggaag
     4381 tggccaccac aagattctgc gcggtctaac gcatcgtgtg ccatcaatgc accgatttcg
     4441 ataaagtcag ccaaacgcaa tgccttgtcc aattccacgt tcaagctgtt ggcctcaccc
     4501 ggaatgaata cattgctcca gaattccttc ttcaggtctt tcaacatttg gatagcttct
     4561 ttcaagcctt ccgcgtcgcg tgccataccg acgtgttccc acatgatatg tcccagctta
     4621 cgatggatag aatcgacagt ttctttaccc ttcacgttca tcaacttcgc gatacgggcg
     4681 ttaatagcct tttctgcttc cacaaattca ggcaggtcag tgctgaagcg cggaacctgg
     4741 atctggtcag acagatagtt ctggatcgtg taaggcaata caaagtaacc atcagccaat
     4801 ccctgcatca aagcagaagc acccagacgg ttagcgccgt ggtcagagaa gtttgcttca
     4861 ccgattgcga acagccccgg aatagaagtc atcaattcat agtctaccca caaaccaccc
     4921 attgagtagt gcaatgcagg ataaatcatc atcggagttt cgtacggatc atcgttcgtg
     4981 atctcttcgt acatatcgaa caggttacca tatttctgtt taactacttc cttacccaga
     5041 cggttgatag cgtcggagaa atccaggaat acagccaagc ccgtgctatt tacaccgaaa
     5101 ccggcatcac aacgttcttt agcggcacgt gaagccacat cacgaggaac caagttaccg
     5161 aatgccggat aacgacgttc cagatagtag tcgcgatctg cttcaggaat atccttacct
     5221 ttttttgttc cggcctgcaa tgccttcgca tcttccagtt tcttcggaac ccagatgcga
     5281 ccatcgttac gcagtgattc agacatcaaa gtcagcttag actggaagtc acctttcacc
     5341 ggcacacaag tcgggtggat ctgcaccata caagggttag caaagtaagc ccctttcttg
     5401 tagcactgcc atgcagcaga accgttagac gccatcgcgt tagtagacaa gaagaatgta
     5461 tttacataac cacctgtcgc aacaacgact gcgtgagcgg caaaacgttc gatcttaccg
     5521 gtaatcaaat tacgggcaat aataccgcga gcacgtccgt caaccttcac aacatccagc
     5581 atttcatggc gtgtgtacat cttcaccttt cccaaaccaa tctgacggct caatgcagag
     5641 taagcaccca atagcagctg ttgtccggtc tgaccacggg catagaacgt acgggatacc
     5701 tgagcaccac cgaatgagcg gttatccaac agaccaccat attcgcgagc gaatggtaca
     5761 ccctgtgcca cacactggtc gataatagag tttgacactt cagccagacg atagacgttt
     5821 gcttcgcggg cacggtagtc accacctttg attgtatcgt agaacagacg ataaaccgag
     5881 tcaccatcat tctgataatt ctttgccgca ttgataccgc cctgtgcag
//
