LOCUS       c00420_gnlPro..         3455 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JIEFPDNF_420.
ACCESSION   c00420_gnlPro..
VERSION     c00420_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:17:25
            Original ID  :: gnl|Prokka|JIEFPDNF_420
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3455
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3455
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[762:3455]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      763..3455
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3455
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3455
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(3..755)
                     /locus_tag="ctg420_1"
                     /transl_table=11
                     /translation="VVCELPQQKRIMDKDKSKIGEFHFVTEPYLLDFRGRVTIPMIGNY
                     LIHVASSHAAERGFGFNDMSERHTAWVLSRLAIEMIEYPAMSEPITLYTWVDEVGRLFT
                     SRCFELVDGNGKTFGYARSIWAAIDVETRRPTLLDIARLSAYVTDRPCPIEKPGKIAAV
                     ENETEGVPYFIKYSDLDINGHLNSIKYMEHLLDLFDLDLFKTKDIKRFEIAYQSEGKYG
                     MKLMLYKREADNGKYDMAICNEGKAICRA"
     CDS             complement(763..1314)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg420_2"
                     /sec_met_domain="ALS_ss_C (E-value: 2.3e-18, bitscore:
                     54.4, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKKLYTVIIFSENTVGLLNQITIIFTRRQLNIETLSVSPSAIQ
                     GIHKFTITTFADEDMIDKVVKQIDKRVDILKAYYNTDEDLVFQEIALYKLSTELFIKMG
                     TVEDLIRKYNARILEMNETCVVLEKSGHYDETQALFRELSETIGVLQFIRSGRVAITKS
                     RVERLSDMLAEMERKLQEKK"
     CDS             complement(1327..3027)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg420_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.3e-52, bitscore:
                     163.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQKITGSEALLKSLIAEGVDTIFGYPGGQAIPIYDSLYDYRDK
                     LKHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGPGATNTITGIADALMDSTPMVVIAG
                     QVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLD
                     LAKDAQIGFVEYEYKKVDYIRSYQPEPDINQDRIKAAAALINKAEKPFCLVGQGVLLGG
                     AEEELKAFLLKNDIPAGSTVLGLSALPSDFPLNKGMLGMHGNVGPNRKTNECDVLIAIG
                     MRFDDRVTGDLKTYAKQAKVIHLDIDNSEIGKNVPVDVKVLGNAKHTIPMITALLEERK
                     RPEWNAEFEPDAQEEYEKVIEKELYPMNGSLKMGEVVRKVSEATGNDAVLVTDVGQNQM
                     MGVRYFKYKRTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELG
                     TIMQEKLDVKIIILNNNFLGMVRQWQELFFHERYSNTIMENPDFVAIAKAYGIAGRTVE
                     KREELDEAIAEMLNHNGAFVLVANVETCGMVYPMVPAGGSVTNMILGDK"
     CDS             complement(3051..3455)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg420_4"
                     /sec_met_domain="ILVD_EDD (E-value: 3.1e-53, bitscore:
                     169.1, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGGTSGLS
                     IGHISPEAAAGGAIGLVKDGDIIEIDIPERTINVKVSDEELAERRKAEEARGTKAFTPP
                     HRQRVVSKALRAYGKMVSSADKGGVRVVED"
ORIGIN
        1 gcagcacggc aaatcgcttt accttcatta cagatcgcca tatcatattt tccgttatca
       61 gcctccctct tgtacaacat gagtttcatc ccatatttgc cttcagactg gtaggcgatt
      121 tcgaaacgtt tgatatcttt tgtcttgaac aggtcaagat caaacaggtc tagaagatgt
      181 tccatatact tgatactgtt caggtggccg ttgatatcaa ggtcgctgta cttaatgaaa
      241 taaggaaccc cttccgtctc attctccaca gccgcaatct ttcccggctt ttctatcggg
      301 caggggcgat ccgtgacata agcgctcaac ctggcgatat ccaacaaggt cggccggcgt
      361 gtttccacat cgatagccgc ccaaatagaa cgggcgtaac cgaatgtctt accgttgcca
      421 tccaccagct cgaaacaacg gctggtaaag agacggccga cttcatccac ccaggtataa
      481 agagttatag gttcggacat ggctggatat tcgatcattt cgatcgccag acgggaaaga
      541 acccaagccg tatgccgttc tgacatatcg ttgaaaccga atccgcgttc agcagcatgg
      601 ctggatgcga catgtatcag atagtttccg atcatcggta tcgttacacg tccacgaaaa
      661 tccaacaggt acggttccgt gacgaaatga aattccccta ttttgctttt atctttatcc
      721 atgattcttt tctgttgggg cagttcgcaa accacccgta cgttattttt tttcctgcaa
      781 cttacgttcc atttcggcca acatatcgct caaacgctcg acacggcttt ttgtgatcgc
      841 aacgcgaccg gaacggatga actgcaagac accgatcgtt tcgctcaact ccctgaaaag
      901 agcttgcgtc tcatcgtaat gaccactctt ttcgagaacg acacacgttt cgttcatttc
      961 cagaatacgg gcgttatatt tgcgaatcag atcctcgacc gtccccatct tgataaacaa
     1021 ctcggtactc agcttataaa gggcgatctc ctggaaaacc agatcttcat ccgtattata
     1081 gtaagccttc aagatatcca cccgtttgtc gatctgcttc acaaccttgt cgatcatatc
     1141 ttcatcggca aatgtcgtga ttgtaaattt atgtataccc tgtatggcgg aaggtgaaac
     1201 ggaaagcgtc tcgatgttca actgccgacg cgtaaagata atcgtaatct ggttcagaag
     1261 acctactgta ttctctgaaa agataataac ggtatataat ttttttgttg tcatagcttc
     1321 acctccttac ttatctccta atatcatgtt cgttacgctt cctccggcag gcaccatcgg
     1381 atataccata ccacacgttt ccacattggc gaccaacaca aacgctccgt tatggttcaa
     1441 catctcggca atagcctcat ccagttcctc ccgtttttca acggtacggc cagcgatacc
     1501 gtacgctttc gcgatcgcca cgaaatccgg gttctccata atcgtattcg aataacgttc
     1561 gtggaagaac aactcctgcc actgacgtac cattcctaag aaattattat tgaggatgat
     1621 gattttcaca tccagttttt cctgcataat ggttcccaac tcctgaattg tcatctgcat
     1681 cccaccgtca ccggtaaaga agcagaccgt acggtcaggt gcaccgaact tggctcccat
     1741 agcagcggga aggccaaagc ccatcgtccc cagaccacct gaagtaacca cgctacgagt
     1801 gcgtttatat ttgaaatagc ggacacccat catctggttc tgtccgacat ccgtaactaa
     1861 aacagcatcg tttccagtcg cctcggacac ttttcgaacg acttcaccca ttttcaaaga
     1921 tccgttcatc ggatacaact ccttctcgat caccttttca tactcttcct gtgcatccgg
     1981 ctcaaactca gcattccact ccggacgttt ccgttcttcc aacaacgcag ttatcatcgg
     2041 aatggtatgt ttggcatttc cgagaacctt cacatcgacc ggtacattct tcccgatctc
     2101 cgagttgtcg atatccaaat ggatcacttt cgcctgcttg gcgtatgtct tcaaatcgcc
     2161 agtaacacga tcgtcgaaac gcataccgat ggcgatcaac acgtcacatt cattggtctt
     2221 cctgttcgga ccgacattgc cgtgcatacc caacataccc ttattcaaag gaaaatcgga
     2281 aggcaacgcc gatagaccga gaacagtcga accggcagga atgtcattct tcagcaggaa
     2341 tgctttcagc tcctcttcag cacctcccaa caaaaccccc tgtccgacca ggcagaacgg
     2401 tttctccgct ttattgatca acgcggcagc cgctttaatc cgatcctggt tgatatccgg
     2461 ttccggctga tagctgcgga tatagtctac tttcttatat tcatactcca caaatccgat
     2521 ctgagcatcc tttgccaaat cgagtacaac aggccccggc cgtccggtag aagcgatata
     2581 aaaagcacgt gaaacagccc acgctatctc ttccggcttg cgaatctggt acgcccattt
     2641 ggtgatcggt tgcgtaatgc cgatcacatc gacttcctgg aaagcatctg tcccaagcaa
     2701 tggagaaggg acctgtccgg ctatgaccac cataggcgtg ctgtccatca gtgcatcggc
     2761 aatgccggtg atggtatttg ttgcgccggg tcctgaagta accaacgtca cccctacttt
     2821 accggataca cgggcatacc cttgtgcagc atgcgtcgcc ccttgttcat gacgcaccaa
     2881 tacatgcttc aatttatccc tgtaatcgta caaactatcg taaataggaa tcgcttgccc
     2941 gccgggataa ccgaaaatcg tatctactcc ttcagctatc agtgacttca gcaaagcttc
     3001 cgaaccggtt atcttctgtt tctccatatc tctttatctt agtcttgtgt ttaatcttct
     3061 actactctca ctccaccctt atcggccgaa ctgaccatct taccgtaagc tctcaaggct
     3121 tttgagacga cacgctggcg atgaggcggc gtaaaggctt ttgtgccgcg ggcttcttcc
     3181 gctttacggc gttctgccaa ctcttcatcg cttaccttca cattaatcgt acgttcaggg
     3241 atatcaatct cgattatatc gccgtctttc accagtccaa tcgctcctcc tgccgcagct
     3301 tcgggagaaa tatgaccgat agaaagaccg gaagtaccgc cactgaaacg accgtctgtg
     3361 atcagcgcgc actccttgcc taaatggcgg ctttttatat aagaagtagg ataaagcatt
     3421 tcctgcatac ccggaccacc cttcggtcct tcgtg
//
