LOCUS       c00225_gnlPro..         5957 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PLIOLNOL_225.
ACCESSION   c00225_gnlPro..
VERSION     c00225_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 15:13:23
            Original ID  :: gnl|Prokka|PLIOLNOL_225
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5957
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5957
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[37:5955]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      38..5955
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5957
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5957
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             38..1837
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_1"
                     /sec_met_domain="ILVD_EDD (E-value: 1.4e-217, bitscore:
                     712.0, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQLRSSFSTQGRRMAGARALWAANGMKKNQMGKPIIAIVNSFT
                     QFVPGHVHLHEIGQLVKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIADS
                     VEYMVNAHKADAMVCISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGEWNGQHLDLID
                     AMIKSADESVSDQEVANIEQNACPTCGCCSGMFTANSMNCLNEAIGLALPGNGTIVATH
                     ENRTQLFKDAAELIVKNAKLYYEEGDESVLPRSIATRQAFLNAMTLDIAMGGSTNTVLH
                     LLAIAHEAEVDFKMDDIDMLSRKAPCLCKVAPNTQKYHIQDVNRAGGIIAIMDELAKGG
                     LIDTSVRRVDGMSLAEAINEYSITSPNVSEKAIKKYSSAAGNRFNLVLGSQGMYYKELD
                     KDRANGCIRDLEHAYSKDGGLAVLKGNIAQDGCVVKTAGVDESIWKFTGPAKVFDSQEA
                     ACEGILGGRVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGG
                     TSGLSIGHISPEAAAGGNIGKIVDGDIIEIDIPARKINVRLTDEELAARPMTPVTRDRY
                     VPKSLKAYASMVSSADKGAVRII"
     CDS             complement(1762..1911)
                     /locus_tag="ctg225_2"
                     /transl_table=11
                     /translation="MTVENEWFTIIALSAAQLTITYQLYYIILTAPLSAELTMLAYAFR
                     LFGT"
     CDS             1952..3646
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 7.9e-48, bitscore:
                     150.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDLITGAEALMRSLEHQGVTTIFGYPGGSIMPVFDALYDHQNIL
                     NHILVRHEQGAAHAAQGYARVSGDVGVCLVTSGPGATNTVTGIADAMIDSTPIVVIAGQ
                     VGTGFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAVARAFYIARSGRPGPVVLDF
                     AKNAQVEKTKYEPTKVDFIRSYVPVPDMDEETVQAAAELINNAERPLVLVGQGVELGNA
                     QSELREFIEKADMPAGCTLLGLSALPTEHPLNKGMLGMHGNLGPNINTNKCDVLIAVGM
                     RFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDVAVLGDCKETLASVTKLLKKNTH
                     TEWIDSFKEYEKVEEEKVIRPELHPATNSLSMGEVVRAVSDATHHEAVLVTDVGQNQMI
                     SARYFKYTKERSIITSGGLGTMGFGLPAAIGATFGAPERTVCVFMGDGGLQMNIQELGT
                     IMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKVASAYDIPSKRAFT
                     REELKEAIAEMLATDGPFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             3697..4254
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_4"
                     /sec_met_domain="ALS_ss_C (E-value: 1.2e-19, bitscore:
                     59.1, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDIIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQENP
                     EASKLIRRYNARIVEVNPVFSIVEKNGMSEDITSLYGELKALNCVLQFVRSGRVAITTS
                     CFERVNEFLDGREAMYNQSKNQ"
     CDS             4260..5003
                     /locus_tag="ctg225_5"
                     /transl_table=11
                     /translation="MNEENRIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHANDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYENFSVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVNYVCDEPCPIEKPSRIKVTSDQSIATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYKTKRIRRFEMAYVAESYFGDELSFFCDEV
                     NANEFHVEVKKNGNEVVCRSKVIFE"
     CDS             5071..5955
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_6"
                     /sec_met_domain="IlvN (E-value: 2.7e-47, bitscore: 149.1,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 4.9e-32, bitscore: 99.9,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTTETVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQREGKTYDKAVADGWVPGETLFGIEEACEKGTIVMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWNDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGKAYERTIALGIGIGSGYLFETTFQREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHSPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLY"
ORIGIN
        1 gtgtgaggaa ggctctttaa ttaaaagata gattgaaatg aaaaagcaat tacgcagttc
       61 gtttagtaca caaggccgtc ggatggcggg agcccgtgcg ttatgggcag ccaacggcat
      121 gaagaagaat cagatgggta aacccattat tgctatcgtc aattcgttca cccagtttgt
      181 tccgggacat gtgcatctgc atgaaatcgg tcagctggtg aaggcagaga ttgagaagct
      241 gggatgcttc gctgcggaat tcaatacaat agctatcgac gacggtatag caatgggaca
      301 tgatggtatg ctttactcgc ttccttcacg tgacatcatt gcagacagtg tagaatatat
      361 ggttaatgcg cacaaggcag atgcaatggt atgtatcagc aattgcgata agattactcc
      421 cggaatgttg atggcggcta tgcgtttgaa tattcctgcc gtatttgtat cgggcggtcc
      481 gatggaagcc ggagaatgga atggtcagca cctggatttg atcgacgcga tgattaaatc
      541 ggctgatgaa agtgtcagcg atcaggaagt agcgaatatc gagcaaaatg cttgtcctac
      601 ttgcggatgt tgttccggta tgtttaccgc caattcaatg aactgcctga atgaagcaat
      661 cggactggct cttcccggaa acggaaccat cgtagctaca cacgagaatc gtacgcagct
      721 tttcaaagat gcagcagaac tgatcgtaaa aaatgcaaag ttatattatg aagaaggaga
      781 cgaaagtgtg cttcctcgta gcattgctac ccgtcaggct ttcttgaatg ccatgacgct
      841 ggatatagca atgggtggtt ctactaatac agttcttcac ctgttggcta ttgctcatga
      901 agcagaggta gatttcaaga tggatgatat cgatatgctt tcccgcaagg ctccttgtct
      961 ctgcaaggtg gctcccaata cacagaaata tcatattcag gatgtaaacc gtgccggtgg
     1021 tattattgct atcatggatg aactggcaaa aggcggtctg atagatacat ctgttcgacg
     1081 ggtagacgga atgtcattgg cggaagctat taacgaatat tcaatcacca gcccgaatgt
     1141 aagcgaaaag gcaattaaaa aatattcgag tgcagccgga aatagattca atctcgtact
     1201 tggttcacag ggcatgtact ataaggaact ggataaagac cgtgcaaacg gatgtatccg
     1261 tgatttggaa catgcttata gcaaagacgg tggattggcc gtattgaaag gcaatattgc
     1321 ccaagacggt tgtgtggtaa aaacagcagg tgtagacgaa agtatctgga aatttaccgg
     1381 acctgccaaa gtatttgatt cgcaagaagc agcctgtgaa ggcatcctcg gaggccgagt
     1441 cgtcagtggc gatgtagtcg tcattacgca cgaaggtccg aagggtggtc ccggtatgca
     1501 ggaaatgctt tatcctacct cttatatcaa atctcgtcat ctcggaaaag aatgtgcttt
     1561 gattaccgac ggacgtttca gtggtggaac ttccggattg agtatcggac atatttctcc
     1621 tgaagcggca gctggaggca atatcggaaa aattgtagac ggagacatta tcgaaatcga
     1681 tattccggcc cggaagatta acgtgcgact gacggatgaa gaactggcgg cccgtccgat
     1741 gactcctgtc actcgtgacc gttatgtacc gaagagcctg aaggcgtatg ccagcatggt
     1801 aagctctgcc gataagggag cagtgagaat tatataatat agttgatagg taatagttag
     1861 ctgcgcagca cttagcgcaa tgattgtaaa ccattcattt tcaactgtca tttctcaact
     1921 atcatttatc atttatcaac tatcaactaa tatgaaagat ttaataacag gtgcagaggc
     1981 tttgatgcgc tccttggaac atcagggagt gactaccatc tttggttacc ccggtggttc
     2041 tattatgccg gtattcgatg ccttgtacga tcatcaaaat atattgaacc atatcttggt
     2101 tcgtcacgag cagggagccg cccatgccgc acaaggttat gcccgtgtat cgggtgatgt
     2161 aggtgtttgc ctggtgacga gcggtcccgg agcaaccaat acggtaacag gcattgcgga
     2221 tgcgatgatc gatagtacgc ctatcgtagt gattgccgga caggtcggaa caggctttct
     2281 tgggacggat gcttttcagg aagttgactt ggtaggtatc acccagccaa tcactaaatg
     2341 gagttatcag attcgccgtg cggaagatgt agcctgggcg gtagcacgtg cgttttacat
     2401 tgctcgtagt ggtcgtccgg ggccggtggt actggacttt gccaaaaatg cgcaagtaga
     2461 gaaaacgaaa tacgaaccga cgaaagtcga ttttatccgt agctatgttc ctgttcccga
     2521 tatggacgag gagactgtac aggctgctgc cgaactgatt aataacgctg aacgtccgct
     2581 tgtattggtt ggacaaggtg ttgaactggg aaatgcacag agcgaattgc gcgagtttat
     2641 cgaaaaagcg gatatgcctg cgggttgtac gctgctcgga ctttcggctt tgccgacaga
     2701 acatccgttg aataaaggta tgttgggtat gcacggcaat ctgggaccga atatcaatac
     2761 caataaatgt gatgtgctga ttgctgtcgg tatgcgtttt gacgaccgtg tgaccggtaa
     2821 tttggctact tatgccaaac aggcaaaagt gattcatttt gatatcgatc ctgcggaagt
     2881 taataagaat gtaaaggtag atgtagctgt attgggagat tgcaaggaaa cactggcttc
     2941 tgtgacgaag ctcctgaaaa agaacactca taccgaatgg attgatagct tcaaagaata
     3001 tgagaaagta gaggaggaaa aagtaatccg tcctgaactt catcctgcca caaactcgtt
     3061 gagtatgggg gaagtggtac gtgcggttag tgatgctact caccatgaag ctgttttggt
     3121 aacagatgta ggtcaaaatc agatgatatc tgcacgttac tttaaatata cgaaagaacg
     3181 cagtatcatt acttccggag gacttggaac aatgggcttc gggcttcccg cagctatcgg
     3241 ggcaactttc ggggcaccgg agcgtaccgt atgcgtattt atgggagatg gcggactgca
     3301 aatgaatatt caggaactgg gtacgattat ggagcagaag gctccggtga agattatctg
     3361 cctgaacaat aattacctcg gaaacgtacg tcagtggcaa gctatgtttt tcaaccgtcg
     3421 ctattcgttt accccgatgt tgaatccgga ttatatgaaa gtggcctcgg cttatgatat
     3481 tccttccaaa cgtgctttca ctcgtgaaga actgaaggaa gctattgctg aaatgctggc
     3541 aacggacggt ccgttcctgc tcgaagcttg cgtggtggaa gaaggtaatg ttttgccgat
     3601 gactcctccg ggtggttcgg taaaccagat gttgctggag tgctaggaga agttgagaat
     3661 ggagagttga gagttcgcat ttaaaaaatt agagttatga gtgataaaac gttatataca
     3721 atcattgttc attcggagaa tatagctgga ttgctgaatc aggtaactgc tgtatttacc
     3781 cgtcggcaaa taaatattga gagtctgaat gtatctgctt cttctattaa aggtgtacat
     3841 aagtatacaa taacagcctg gactgataag gacatcattg agaaggtagt gaaacagatc
     3901 gaaaagaaga tcgacgtgat tcaggcacat tatttcacag aggatgagat ttatttccac
     3961 gagattgcat tatataaggt gtctactcct gcttttcagg agaatccgga agcatccaag
     4021 ctgattcgta gatacaatgc acgtatagtg gaagtcaatc cggtattttc tattgtcgag
     4081 aaaaatggaa tgagcgaaga tataacttcg ctctatggtg aactgaaggc tttgaattgt
     4141 gttttgcagt ttgttcgttc aggacgtgta gcgattacta ccagctgttt tgaacgggtg
     4201 aatgagtttc tcgatggacg ggaagctatg tataaccaaa gtaaaaacca ataagaataa
     4261 tgaatgaaga aaatagaata gggacttatc agtttgtggc agaaccgttc catgttgact
     4321 tcaacggacg tctgactatg ggggtactgg gaaatcatct actgaattgt gcaggttttc
     4381 acgccaatga ccgtggtttt ggtattgcaa cgctgaatga ggacaattat acatgggtgc
     4441 tttcacgtct ggctattgag ttggatgaaa tgccttatca gtatgagaat ttctcggttc
     4501 agacgtgggt ggaaaatgtc taccgtttgt ttacggaccg taattttgct attattgata
     4561 aggatgggaa gaagattggc tacgcacgtt ccgtatgggc aatgattaat cttaataccc
     4621 gcaagcctgc cgacttgttg actttgcatg ggggaagtat cgtaaactat gtctgtgatg
     4681 agccttgccc gattgaaaag ccttcacgta tcaaagtgac aagcgatcag tcgatagcta
     4741 ccctgacggc aaagtacagt gatatagata tcaatggtca tgtaaacagc atccgttaca
     4801 tcgaacatat ccttgatttg ttcccgattg aattgtataa gaccaaacgt atccgtcgtt
     4861 ttgaaatggc gtatgtggcg gaaagctatt ttggtgatga actctctttc ttctgcgatg
     4921 aagtgaatgc aaacgagttt cacgtagaag tgaagaagaa tggcaacgaa gttgtttgcc
     4981 gctcgaaagt gatatttgaa taaatgtgag aatataaaat caattaataa cagatggcgt
     5041 aagtcatcaa aaactaaaaa caaaaagaaa atggcacagt tgaattttgg aggtactact
     5101 gaaactgtag taatccgtga cgaatttcca ttggaaaaag ctcgtgaagt attgaaaaat
     5161 gaaacaatcg ctgtaatcgg ttatggcgta cagggtcctg gacaggcact gaacttgcgt
     5221 gataacggtt tcaatgtaat cgttggtcaa cgtgaaggaa aaacatacga taaggcagta
     5281 gcagacggat gggttccggg tgaaactttg ttcggtatcg aagaagcttg tgagaaaggt
     5341 actattgtaa tgtgtctgtt gtctgacgca gcagtaatgt cagtatggcc tactatcaag
     5401 ccttacctga ctgccggaaa agctctttat ttctcacacg gttttgctat cacttggaac
     5461 gaccgtacag gtgtagttcc tcctgctgat atcgacgtta tcatggttgc tcctaaaggt
     5521 tcgggtacat ctttgcgtac tatgttcctc gaaggacgtg gcttgaactc ttcttatgct
     5581 atctatcagg atgctacagg caaggcttac gaaagaacta tcgcactagg tatcggtatc
     5641 ggttccggtt atctgttcga aacaactttc caacgtgaag ctacttctga cttgacaggt
     5701 gaacgtggtt cattgatggg tgctatccaa ggtttgttgc tggcacagta cgaagtattg
     5761 cgtgaaaacg gtcatagtcc ttctgaagca ttcaatgaaa cagtggaaga actgacacag
     5821 tcattgatgc cgttattcgc aaagaacggt atggactgga tgtacgctaa ctgctctact
     5881 actgctcaac gtggtgctct cgattggatg ggcccattcc acgatgctat caaaccggta
     5941 gttgaaaagt tgtataa
//
