LOCUS       c00898_gnlPro..         5292 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OIKMCNEA_898.
ACCESSION   c00898_gnlPro..
VERSION     c00898_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 15:48:31
            Original ID  :: gnl|Prokka|OIKMCNEA_898
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5292
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5292
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[217:3176]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      218..3176
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5292
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5292
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..100)
                     /locus_tag="ctg898_1"
                     /transl_table=11
                     /translation="MGRSQRAVITSKYTFLIFFCGIICKLKNDSYLC"
     CDS             218..2446
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg898_2"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2451..3176
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg898_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEAGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
     CDS             complement(3409..4668)
                     /locus_tag="ctg898_4"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVVTTAVSLYFLNSPVADEIRYATAPSLAVNPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLVEKRPEAVTEEKRSEPQQEAIAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSENFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVVDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(4723..5271)
                     /locus_tag="ctg898_5"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
ORIGIN
        1 tgcaaaggta cgaatcattt tttaacttgc aaattattcc gcaaaaaaat attagaaaag
       61 tatatttgga agtaataacc gccctctggc ttctccccat aaataggtat ttttatgcta
      121 ttcagcataa aataggaccc ttttatatac ctaaaccgaa aaaaggttgt aaatttgcgc
      181 gctctaaagg ataactaacc actaactgat taattttatg gaactgaata agacctttaa
      241 agatggactt tggagcaaag aaatcaatgt aagggatttc gttagtaaca acatcactcc
      301 ttatgaaggg gatgcttctt tcctgcaagg acctaccgaa cgcaccaaag ctgtctggaa
      361 tcactgtctc aaagctctcg aagaagagag aaacaataat ggtattcgtg cactcgacta
      421 cacaactgta tctactataa cctctcaccc tgccggctac atcgataaag agaacgaact
      481 gatcgtcgga ctacagaccg atcaggtgtt aaaacgtgcc atcaaaccgt ttggcggaat
      541 caatgtggtt atgaaagctt gccgtgaaaa cggagtggaa gtagatgaca gagttaagga
      601 tatcttcact cactatcgca agactcataa tgatggggta ttcgatgtgt ataccgaaga
      661 aattcgttcg ttccgttcat tgggattcct taccggactt cccgacaact atgcacgcgg
      721 acgtatcatc ggtgattatc gccgcctggc tctctatggt cttgaccgtc tgatagaagc
      781 taaaaaagaa gatctgcata atctgacagg tcctatgact gaagcccgca ttcgtcttcg
      841 tgaagaagta gccgagcaaa tcaaggccct gaaagagatt aaagtattgg gcgaatatta
      901 tggtttggat ctgagccgac cggcatatac cgcacaggaa gctgtacaat gggtgtatat
      961 ggcttatctg gctgccgtaa aagagcagga tggtgctgcc atgtcactgg gtaatgtatc
     1021 ttcattcctc gacatctatc ttgaatatga attaagccag ggtacgataa ccgaaacatt
     1081 cgctcaggaa ctgatcgacc aattcgttat caaactccgt atggtacgcc acttgcgtat
     1141 gcagtcgtac aacgatattt ttgcgggtga tcccacatgg gtgaccgaat ctatcggtgg
     1201 acgtttcaat gacggacgta ccaaagttac taaaacttct ttccgcttct tgcagacact
     1261 ttataactta ggcccgtcac cggaacctaa catgactgta ctatggagcc ctgagttacc
     1321 cgaaggcttc aaagcattct gtgcacaagt atctatcgac acttcttccg tacaatacga
     1381 aaacgacgac ctaatgcgtg aagtccgtca atcggatgac tacggaatcg cttgttgtgt
     1441 ttcatatcag gaaatcggaa agcagattca gttcttcggc gcacgtgcca accttgccaa
     1501 agccctgcta ctggctatca acggaggacg ttgcgaaaat acaggtacag ttatggtaaa
     1561 agatatcccc gtattgaccg gtgaaacact gaagttcgaa gaagtaatgg ccaactataa
     1621 gaaagtattg atacaaattg cacgtgtata caacgaagca atgaatatca tacattacat
     1681 gcacgataag tattactacg aaaaggcaca gatggctttc gtggatactg atccccgcat
     1741 taacctcgct tatggtgtag ccggactttc aatcgccatc gattcactgt ccgccatcaa
     1801 atatgcacat gtgaaagccc gtcgcaacga catcggactg acggagggat tcgatatcga
     1861 aggtgctttc ccttgtttcg gtaatgacga tgaccgtgta gaccacctgg gtgtggacct
     1921 ggtatatttc tttagcgaag aattaaagaa actccccgta tataagaatg cccgtcctac
     1981 cctttcgctg ctgactatca cttcgaacgt aatgtatggc aagaagaccg gtgccactcc
     2041 tgacggacgc gccaaaggca ttgcctttgc tcccggtgcc aaccccatgc acggacgtga
     2101 taaaaatggt gcaatcgcct cactgagttc tgtagccaag ttgcgttatc gcgactcaca
     2161 ggatggtatc agcaatacat tctcgatcgt tcccaaatca ttgggagcaa cacaagagga
     2221 ccgtatcgac aatctggtga ccatgatgga cggatatttt acaaaaggcg cacaccacct
     2281 gaacgtaaac gtgctgaacc gggaaatgtt gcaggatgcg atggaacatc ccgaaaagta
     2341 tccgcaactg actatccgtg tctcaggata tgccgttaat ttcgtgaaat tgagccgcga
     2401 acatcagttg gaagtaatca gccgcagttt ccacgaaaga atgtaattga atgataaacg
     2461 tacactcata cgagtcaatg ggaacattcg acgggccggg tctccggctc gtcgttttcc
     2521 ttcaagggtg taacttccgt tgcctctatt gtgccaatcc ggacacaatt gaagccaaag
     2581 gagggacagc aaccgaccct gaggaaatag ttcgcatggc cgtcagtcag aaagctttct
     2641 tcggtaaaaa gggaggtatc accttttccg gtggagagcc tacttttcaa gcaaaatcgc
     2701 ttatcccact ttttaaaaga ctaaaggaag caggaatcca tatttgcctt gataccaatg
     2761 gcgggctatg gaacaatgat gtagaggaac tattggaatt gactgatctg gttttattag
     2821 atatcaaaga atttaatccg gaacatcatc agtctttaac cggaagaagc aacgagcaga
     2881 ccctgaaaac cgcagcctgg cttgaaacca atcataaacc attctggttg cgatacgtat
     2941 tagtgcccgg ctacagtgac ttcgaagacg atatccggca gctgggagag catcttggaa
     3001 cgtaccagat gattcagcgt gtagaaatat tgccttacca caccttgggt gttcacaaat
     3061 atgaagcaat gaacaaagaa tatatgctga aaggagtgaa agagaatact ccggagcaga
     3121 tagaaaaggc tgaaaaacta ttcaggcaat acttccggac cgtacaagtg aattgactta
     3181 tataagtgtc gaaattcgta ttcgccacag attacacgaa tttacacaga taaagattca
     3241 ttggcaatca acagattaag ttcattctgc ggtgaattat gacgactgca cttatttata
     3301 attttgtaag tcacgatata aaagtctccc tgttcttgcc ttaattcatt acaatacaag
     3361 gcaagaacag ggagataatg taaccagcat attttttgcc atcctacttt aataagtgaa
     3421 tcgcaatcct gcctgcaagt taaagttaca aggcctttct tttcggatgg tttgtacttt
     3481 ggagccatca tcaaagaaat acgaaactcc cggttccaca tataggccga catgatctgt
     3541 cgcattgaat tgcgctccca cggcaccggc tacagaaaat tgcaaaggct tcacattcac
     3601 cttatcatcc gccaactttc cataaacaca cttttcaacc attcctccgg cagatacata
     3661 gagtgtaaaa tatttctttt ccagaaaatt ccagtttcca cgtacaggaa tacctatata
     3721 atgcaatttc tgactctgca tcttggtatc cccctgccgt ttgatgtcag aagacaacaa
     3781 cgtataagtc aatcccgtct ctacagagaa acctttggga agagacttac gtacagacag
     3841 tccgaagcta accggctgat gatgttcata atccaccacg gcagttgtat tggacttcag
     3901 gtaagggact ccttcttcaa atataacttc ctgatcggca ggaatactca cagctccatt
     3961 catgatcgtc atcaagtcga ccctgttagt catcggacgg ggcgcaaaat tttcggaacc
     4021 atttgtcgga agtccacctc cattaccgat acccactccc atcgaccatt taccaccacg
     4081 tttagcagaa gagtctccga taggcaactg atacttgtct ttaccggacg gtcgccgcgc
     4141 ttctttcctc tttggaggct gcgccgtcgc actttctttt ttctcgattg cagcaattgc
     4201 ttcctgttgc ggctcagatc tcttttcctc cgtcactgcc tccggtcgct tctctaccag
     4261 ggaggatacc tccggaacaa tcaccggatc agtattcttt gccaaatagc ccgaaacagg
     4321 gttaatactc gtcgttccta ccggcttaac gggttcggat acagctacct gtacatccgg
     4381 caaggcaggt tccggcaata catccgggtt gacagccagt gaaggagccg ttgcataacg
     4441 gatttcatca gctaccggac tattcagaaa atacaaactg acagcagtcg ttaccaccaa
     4501 taccacagct gccgcagcaa ccgcccaccg gcgatacgga tatattcgtt tttcggttac
     4561 aggcatcagt tccctttcca gtcgttccca accggaagca ggcatcggct ccgaataatc
     4621 ttcgagctta tccttgaatg ctttaatcca tttttcgtct tctttcatca ttctatttca
     4681 tttttgaaat ccaaggttca cctcagtatc tgtaaataca tgtcaaccat tggtcaccaa
     4741 ccattctttc actttctttg ccaatacact ttttgcgcga aaaagctgtg aggcagaaga
     4801 tttttcatta atccccaaca tctgtgcgat ttccttgtgc gacttatctt caaaggtgta
     4861 taaattgaat acggtacgat atccggcggg gagttcatta ataaattgca tcagcacttt
     4921 ctgtggtatt gcttcaacag ccgaagcatc cggttcttca tacgtttcgg gaacttcatc
     4981 cagtgccgtg gtctggttca tcacgtcgtt ctttcttaag aactgtaaag ccgtattcac
     5041 cattacacgc tccatccatg ctctcagaga gccttcaccc cgccaggtaa acttgtcgaa
     5101 ggagtcgaaa atcttcagga aaccgtcgtg taccaagtct tgtgccatgt ccctgtctcc
     5161 ggcataacga agacagacac caagcatgcg ccctgcgtac tgctcataaa gttctttgcg
     5221 ggcacgatta tctccctgcc tgcaacgttc cgacagctct aattcattca ttctcctaaa
     5281 cacgtgttta cc
//
