LOCUS       c00009_gnlPro..         7359 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LJHAIICF_9.
ACCESSION   c00009_gnlPro..
VERSION     c00009_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 11:46:59
            Original ID  :: gnl|Prokka|LJHAIICF_9
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7359
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7359
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[415:3395]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      416..3395
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             416..2656
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg9_1"
                     /sec_met_domain="Gly_radical (E-value: 5.6e-46, bitscore:
                     144.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1e-282, bitscore:
                     927.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVERKKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRLGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLVDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             2673..3395
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg9_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(3398..3703)
                     /locus_tag="ctg9_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCKGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             3980..4546
                     /locus_tag="ctg9_4"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     NEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             4551..6116
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg9_5"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1e-48, bitscore:
                     154.9, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSAMKDQLSDIFSGLVSQYVFDIQVAPDHESRQELIDLLGDVA
                     SCSDHLSCSVADGEGLQFTIVKDGMPTGIRFRAVPNGHEFTSLLLAVLNSDGKGKNIPD
                     ESICARVRALNGPIKLTTYVSLTCTNCPDVVQALNVMATLNSRITHETVDGAINQKEVE
                     AMKVQGVPSVFADGKLINVGRSDLGELLSKLEAQYGINEGGVDNSVEKPVKNYDVLIVG
                     GGPAGAASAIYSARKGLNVAVIAERIGGQVKETVGIENLISVPQTTGKQLANDLLMHIN
                     DYPVDILEHRRVDKVELDGSAKLLTTSTGERFSAPALIVATGASWRKLNVPGEADYIGK
                     GVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEK
                     AKSLPNVEVFLHSQSLEVLGNGDKVTGLRVKDRKTDAERVIDLDGIFVQIGLAANSGVF
                     RDVVETNKPGEIVIDAHCRTNVPGIYAAGDVSTVPYKQIIIAMGEGAKAALSAFEDRMR
                     GLIG"
     CDS             complement(6396..7067)
                     /locus_tag="ctg9_6"
                     /transl_table=11
                     /translation="MEKSEELQIATSGKNMDIHRMELLPVVCLSVYKEIIKEMAELIED
                     KQIREIFCSLSLGKDLLCVSQITGISSKNLVSMYKRGIRQIHLKWKPYSELKRELDHIY
                     IKCRNNELLLTHSEEVSKKNFRYVKIVLKEQDIPTEYVDLLSTPLSKLDINSRTLRAFR
                     KYNIYQLEDLLRFIKYNGFEALYQMPGIGTKSIEQLYEKLKDKKILVDQDTCFLFPYLF
                     V"
     CDS             complement(7078..7359)
                     /locus_tag="ctg9_7"
                     /transl_table=11
                     /translation="TNSIKELANLCHISERSFNRKFHSCFGESPYKWIQKKKAEQIQEM
                     INDPEFSFKEIAEELGFSSSPHLTTYCQRMFGMTPSQLREKNKDKWIK"
ORIGIN
        1 aaaaaacaat actgtctatc gtaagggtgg ttagcgaacc acccttacac aggcacaatg
       61 acattttata atcaaataat tctagacaac ctgcgtgaaa ccgtatgtaa gaggttttct
      121 tgatctatgg tttcaattac cctgcgacag gcgaacaagc ggttggattt aatattaata
      181 atactaaagt aatgaaatta aaagttctac tgcttttccc tctgtgaaga ccggacggca
      241 gacaaaaaac aggccccggc ggtattcgta ctgccggggc ttcgttttta tttcccttat
      301 ccctatttgt aggtattttc tccggtttaa catccctcgt cctttacaat ccttttcaga
      361 tagggggaaa agacgtatct ttgcgtgcct aaaccgaaat agacaagata agcttatgaa
      421 gtttgaaaac gagaactggg cgcagttcaa aggagaaacc tggaaaaagg agatcaatgt
      481 aagagacttt atccagaaca attacaaacc ttataacgga gacgatacgt tcctcgtacc
      541 ctcttcggag aaaacgaaga aagtgtggaa caaactaacc gagatgttta aggttgaaag
      601 gaagaaagga gtatatgatg ccgaaacgaa gcttccccag gggattgaca catacggacc
      661 cggttacatc gataaggaaa acgaagtaat cgtcggcttg cagacagacg ccccgttgaa
      721 acgcggtatc ttcccgaaag gcggtatccg catggtcgaa aacagtctgg aagcctatgg
      781 ctatcatctg gacccgatga caaaagaaat cttcaccaaa tacagaaaga cgcacaacga
      841 aggcgttttt tcagcctata cagacgagat gattgcggca cgtcgttccg ccatcattac
      901 cgggttgcct gatgcctacg gacgcggacg tatcatcggt gattaccgtc gtgtacctct
      961 gtacggaacc cagatcctga tcgaagaaaa gaaacgtttc cagaaacgtc tcgatataca
     1021 ggaactgaca gaagaaatca ttcagagtcg tgaggagata acggagcaga tcaaagcgct
     1081 gaaagcattt gagaaaatgt gtgccggtta tggtttcgat gtgacacgtc cggcaaagga
     1141 tgcccgcgaa gccgtgcagt tcttgtatct ggcttacctg gcagccgtaa aggatcagga
     1201 tggagcagcc atgtctctcg gacgtacttc tactttcctc gatatctata tcgaaaaaga
     1261 tatttgcgaa ggcaagctga cggaagaaga ggcacaggaa ttgatcgacc agtttattat
     1321 caaactgcgt attgtccgtt tcctgcgtac tccggaatac aacgacctgt tctccggcga
     1381 ccctgtttgg gtgactgaat cgctgggcgg acaaggtgtt gacggccgtt cgctggtgac
     1441 aaagacatct taccgttatc tgcatacctt gtataacctc ggcccggcac ccgaacccaa
     1501 cctgacggtg ctttggttca acaatgctcc ggataactgg aaaaacttct gcgcaaaagt
     1561 atcgatcgat acctctgcca tccagtacga gaacgacgat ctgatgcgtc cggactatgg
     1621 tgacgattac ggtatcgcct gctgtgtgtc tccgatgaag atcggtaagc agatgcagta
     1681 tttcggagct cgtgccaacc tggcaaagtg cttgttgtat gcgatcaatg gcggacgtga
     1741 cgagaaatcg ggtgtgcagg tggctccccg tttcgaaccg atcaccagcg agtatctgga
     1801 ttacaacgaa gtgatggaga aatacgagca gatgatgcgc tggttggcaa aagtttatgt
     1861 caatgctctg aagatcattc actacatgca cgacaaatat gcgtatgaag cattcgagat
     1921 ggggcttcat gacggagatg tggaacgtat ccgcgcaacc ggtatcgccg gcctttctat
     1981 cgttgccgac tcgctggctg ccatccgtga cacgaaagtg aaagtgatcc gtgacgaacg
     2041 tggtttggct gtcgacttcg aacgcgaagg cgaatatgta cctttcggaa acaatgacga
     2101 ccgcacggac agtattgctg tcgacattac cgagaagttc atggaatatc tgcgccagca
     2161 tcagacatac cgcaaggcaa ctccgacaca atctatcctg acgatcacat caaacgtggt
     2221 ttacggaaag aaaaccggaa cgactcccga cggccgtctg ggtggtacgc cgttcgctcc
     2281 gggagccaac ccgatgaacg gacgcgacac gaaaggggct gtcgccgctt tggcttctgt
     2341 tgccaaactg cctttccagc atgcgcatga cggtatctcc tatacatttg ccgtttcacc
     2401 ggctacattg ggtaaggagc gcgatattca gataaataat ctggtgagcc tgttggatgg
     2461 ctatttcacg cctgacggcg gccagcatct gaatgtgaac gtgttcgata aggacctgtt
     2521 ggtcgatgcg atggagcacc cggagaaata cccgcagctt acgatccgtg tttctggcta
     2581 tgccgtcaac ttcgtgaagc tgacacgcga acagcagctt gacgtcatct cgcgcaccat
     2641 caacagcagt ttataattgt ccattgatta atatgttggg gcgtatacat tctttagaga
     2701 gtttcgggac cgttgacggt cccggaattc gctttgtcgt ttttatgcag ggatgtccgc
     2761 ttcgttgtct gtattgccac aatccggata cctgggaggt aaagcgtgaa acgccttatc
     2821 tgctggaacc tgaagcgttg cttgccgaag tgctgcgcta taagaatttt atcgctaaag
     2881 gtggtgttac cgtgaccggt ggcgagcctt tgttacaacc tgaattcctt aaagagtttt
     2941 tccgcctttg ccgtgagaac ggtattcata cggcgttgga cacttccggc tatatctgtt
     3001 ccggtaaagc attggaggtg ctcgaacagg tcgatctggt tctgttagat atcaagacga
     3061 tcgacgccgg tcttcatccc cgtttgacgg ccgtaaaact cgataatact ttacgctttc
     3121 ttgatgaact ggagaaacgc ggcatccctg tatggattcg ccacgtgatc gtgcccggcc
     3181 tgacggacga tgatgaagcc ttgagcaagc tggccgaata tatcagttca tataacgtgg
     3241 tccagaaagc cgaacttctg ccttatcata caatgggagc ttacaaatat gaggcgcaag
     3301 gcctggacta taagctgaaa ggtgtggaac cgctttcagc agaacgtctg gccaatgcga
     3361 aagcgatctt tatgaaacac ggggtaaccg tataaaccta atcgagtgtc tgctctttga
     3421 tgatgatcgg tttacggctg atccggttga ctgtgcggac gctgtcgctt tcaatactgg
     3481 taacatctcc cttgcagttg gtgaaataga tgtaatgtcc catatccatg aaacgataga
     3541 ccttacaccc ttcgtgttcg aacagatatt cgacttgata agtggaattg tttaccggtt
     3601 tttcctttgt aaggggtatt tgtgagacac atgaagaaag acatgcgttg caaacgagca
     3661 atagacttgc tgaaaatgta ttatacaaac tagctcttct catcttcgaa tatttttaag
     3721 ttaacatcca aagttaacaa ttaaagaatc acatggtatg atcgaaaggt attttaccta
     3781 caattaggta tatttttatt gttccggaaa acattacttt gcttcctatt gtcatagtag
     3841 ataagtatgc ttgcagatag aaatccgtct atcggcagat aaaaattatc aatttgaaaa
     3901 attctttcaa tcagtatctt acgatttacc tttgtagggt aaacaaaaaa tagtagtaac
     3961 aaattaaata gtaaaaatca tggaaccaat tatcaattca caagtacccg aattcaaagt
     4021 acaggctttt cagaatggta gtttcaagac agtaagtagc gaagatatca agggcaaatg
     4081 ggctatcttt ttcttctatc ctgccgactt tacgttcgta tgtccgaccg aattggtcga
     4141 tatggctgac aagtacggca aatttcagga aatgggtgtc gaagtatatt ccgtaagtac
     4201 agactcgcac tttgtacaca aagcatggca tgatgcttcc gaaacgatcc gtaagatcaa
     4261 atatccgatg ctggcagacc cgacaggggc tttaagccgt gcgttcggtg tgatgattga
     4321 agaagagggg atggcttatc gcggtacgtt cctggtaaat cccgagggaa agatcaagat
     4381 tgccgaaatc cacgataacg gtatcggccg taatgcagac gaattgcttc gtaaagtcga
     4441 agctgcccag ttcgttgctt ctcacccgaa cgaagtttgt cctgcaaagt ggaaaaaagg
     4501 cgaagcaacc ttgaagccga gcatcgactt ggttggtaag atctaaagtt atgttagact
     4561 cagcaatgaa agaccagctt tcggatattt tctccggact ggtatctcaa tatgtatttg
     4621 atatacaagt cgctcccgat cacgaaagcc gtcaggagct aatagacctg cttggtgatg
     4681 tggcatcatg ctcggatcat ttgtcatgca gcgttgcgga tggcgaagga cttcagttta
     4741 caattgtaaa agacggtatg cctaccggta tccggtttcg tgcagtacct aacggacatg
     4801 agtttacatc cttgttgtta gctgtcctga atagtgatgg aaaaggtaaa aatataccgg
     4861 acgaaagtat ctgtgctcgt gtcagagcct tgaatggtcc gatcaagctg acgacttatg
     4921 tttctcttac ttgcaccaat tgtccggatg tagtgcaggc actcaatgtc atggctactc
     4981 tcaatagccg gatcacccac gaaacggtgg atggcgctat caaccaaaaa gaagttgagg
     5041 caatgaaagt acagggagtc ccttctgttt ttgccgatgg caaactgatc aatgtggggc
     5101 gtagtgattt gggagaattg ctttccaaac tcgaagccca atacggaatt aatgaagggg
     5161 gtgttgataa ctctgttgaa aaacctgtta agaactatga tgtgttaata gtggggggtg
     5221 gacctgccgg tgcagcttct gctatctact cggcacgtaa aggcttgaat gtcgcagtga
     5281 tagccgaacg tatcggagga caggtcaaag aaacggtcgg tatcgagaac ctgatttccg
     5341 ttcctcagac tactggaaag cagttggcga acgaccttct gatgcacatc aacgactatc
     5401 cggtcgatat actggagcat cgccgtgtgg ataaagtcga actggatgga tcggcgaaac
     5461 ttctgaccac ttctaccggt gaacgttttt ctgctcctgc ccttatcgtg gcgacaggtg
     5521 caagctggcg caagttgaat gttccgggcg aagcggacta tatcggaaaa ggtgtcgctt
     5581 tttgccctca ctgcgatggt ccgttctata aaggtaagca cgtagcggtt gtcggcggtg
     5641 gaaattcggg tatcgaagcg gctatagatt tggctggcat ttgttcgaaa gtgacggttc
     5701 tggaatttat ggacgaactg aaagccgacc aagtgttgca ggaaaaagca aagagcttgc
     5761 cgaatgtgga agttttcttg cattcccaat ctttggaagt gctcggtaat ggcgataagg
     5821 tgacaggtct tcgtgtaaaa gatcgtaaga cggatgcgga gcgggtgatc gacctggacg
     5881 gaatctttgt ccaaatcggc ttggcagcca acagtggtgt tttccgcgat gttgtcgaaa
     5941 cgaacaaacc gggtgagatt gtaatcgatg cgcattgtcg gacgaatgtt cccggcatct
     6001 atgcggctgg cgacgtctcg accgtaccct acaaacagat aatcatagca atgggagaag
     6061 gagcgaaagc agctctgtct gcttttgaag accgtatgag aggtttaatc ggttaatatt
     6121 cgtatttgct atgaatgagg ggaggatatg tttccgttct ctatgcctaa taccttcttc
     6181 tgtgagtatc ccttttcgag ccataaccag agttgattac accaatagct ggtaagtagt
     6241 tgtaacggtg cttggaggaa gggctgaaga gaagattaga gataatagta actatttcac
     6301 aagttttttg tttccgatgc cgacttatcc gatactacga aagactgtgc attgtctacc
     6361 tacaaaatgt attattgtat atcagtgtga catatctata caaaaagata agggaataaa
     6421 aaacaagtgt cttgatctac taaaattttc ttatccttca gcttttcata taattgctca
     6481 atagatttag tgcctatgcc aggcatttgg tataaagctt caaatccgtt atatttaatg
     6541 aaccttaaaa gatcttccaa ttggtagata ttatattttc tgaatgcacg taaagtcctg
     6601 gagtttatat ccagtttact taaaggggta cttaatagat ccacatattc cgttggaata
     6661 tcctgctctt tcaaaacaat ctttacatac ctgaaattct tttttgaaac ctcttcagaa
     6721 tgtgtcaaaa gcaattcatt atttcggcat ttgatatata tgtgatctag ttctcgtttt
     6781 aattctgaat aaggtttcca cttcaaatgg atctgtcgga tccccctctt atacatggaa
     6841 accagattct tggacgaaat gcctgttatc tgtgaaacac atagaagatc ttttcctaaa
     6901 gatagtgaac aaaatatttc ccgtatctgt ttgtcctcaa tcaattctgc catctctttt
     6961 ataatctctt tgtaaaccga taagcatacc actggcaata gctccatacg gtgtatatcc
     7021 atattctttc ctgaagttgc tatttgaagc tcttccgatt tttccataaa aaacaattta
     7081 tttaatccat ttatctttat ttttttctct taattggctg ggggtcatcc caaacatcct
     7141 ttgacaatat gttgttagat gtggcgaaga actgaagccc agttcttcgg ctatttcctt
     7201 aaaagaaaat tccggatcat taatcatctc ctgtatttgt tccgcttttt tcttctggat
     7261 ccatttataa gggctttccc caaaacaaga atgaaacttc cggttgaaag atcgttctga
     7321 tatatggcat aagttagcaa gttccttgat tgaatttgt
//
