LOCUS       gnlProkkaLCIAKKDL_459   4541 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LCIAKKDL_459.
ACCESSION   gnlProkkaLCIAKKDL_459
VERSION     gnlProkkaLCIAKKDL_459
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 13:09:55
            Original ID  :: gnl|Prokka|LCIAKKDL_459
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4541
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4541
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2284:3529]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2285..3529
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..4541
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4541
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /tool="antismash"
     CDS             2..1528
                     /locus_tag="ctg454_1"
                     /transl_table=11
                     /translation="MMIADKSINLDTLHKVLFDNEKLELSEECIRKVEESFDFLQSFSS
                     DKIIYGINTGFGPMAQYRIEDQSLIDLQYNIIRSHSTGAGKPLPELYVKAAMIARLYTF
                     LQGKSGVHLELVSLLCEFINRGIYPFIPEHGSVGASGDLVQLAHIALTLIGEGEVFYQG
                     KLCNAATVLQENGLKPFSMRIREGLSVTNGTSVMTGIGIVNLIYAKKLLRWSVAASVMM
                     NEIAASYDDFMAQALNEAKHHKGQQEIAAMMREWVAGSKCVLQRENELYNQVHKEKIFE
                     HKVQPYYSLRCVPQILGPIYDELENAEEVLINEINSACDNPIVDPDTQNIYHGGNFHGD
                     YISFEMDKLKIAVTKLTMLCERQINYLFHDRINGILPPFVNLGVLGLNYGLQASQFTAT
                     STTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALLAKTVIENSYQVMAIQFMGMAQA
                     IDYLKIQDRLSSKSRQVYEEIRSFFPVFTNDTPKYKEIEMMIDYLKKEDK"
     CDS             1530..2258
                     /NRPS_PKS="Domain: PKS_KR (3-169). E-value: 8.1e-16. Score:
                     50.3. Matches aSDomain: nrpspksdomains_ctg454_2_PKS_KR.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     214.9; E-value: 2.1e-65)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg454_2"
                     /sec_met_domain="adh_short (E-value: 1.8e-35, bitscore:
                     111.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYALVTGGSRGIGRAVSCKLAEMGYFILINYQNNDAEAEKTLQL
                     VQEKGSNGELMKFDVTDPAAITLALGNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEE
                     WSKVLDISLNGFFNVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQTNYSAAKGGVIAA
                     TKALAQEVAKKKVTVNAVAPGFIRTDMTEGIDENEWKKHIPAGRFGTPEEVADLVGFLA
                     SPASSYITGEVISINGGLYT"
     aSDomain        1539..2036
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg454_2_PKS_KR.1"
                     /evalue="8.10E-16"
                     /label="ctg454_2_PKS_KR.1"
                     /locus_tag="ctg454_2"
                     /protein_end="169"
                     /protein_start="3"
                     /score="50.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ALVTGGSRGIGRAVSCKLAEMGYFILINYQNNDAEAEKTLQLVQE
                     KGSNGELMKFDVTDPAAITLALGNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEEWSK
                     VLDISLNGFFNVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQTNYSAAKGGVIAATKA
                     LAQ"
     CDS_motif       1542..1604
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg454_2_0001"
                     /evalue="2.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg454_2"
                     /protein_end="25"
                     /protein_start="4"
                     /score="13.5"
                     /tool="antismash"
                     /translation="LVTGGSRGIGRAVSCKLAEMG"
     CDS             2285..3529
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (65-404). E-value:
                     1.4e-53. Score: 174.1. Matches aSDomain:
                     nrpspksdomains_ctg454_3_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 295.7; E-value:
                     9.8e-90)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg454_3"
                     /sec_met_domain="APE_KS1 (E-value: 3.3e-160, bitscore:
                     521.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVVITGMGIYSCIGKNLDEVKDSLYHGKSGIGIDPVRKELGYF
                     SALTGILERPDLKKLLDRRKRLCLPEQGEYAYLATLEAFRNAGIDENFLESNEVGILYG
                     NDSSAAPVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACA
                     SGSHAIGMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAAPEKASKPFDK
                     RRDGLIPSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQM
                     AIKDAGIALEQISYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAG
                     ASEVIYSTLMMNNGFIAPNLNFEEPDEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNST
                     LIIRKINXXXXKTDI"
     aSDomain        2480..3496
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg454_3_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.40E-53"
                     /label="ctg454_3_PKS_KS.1"
                     /locus_tag="ctg454_3"
                     /protein_end="404"
                     /protein_start="65"
                     /score="174.1"
                     /tool="antismash"
                     /translation="RLCLPEQGEYAYLATLEAFRNAGIDENFLESNEVGILYGNDSSAA
                     PVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACASGSHAI
                     GMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAAPEKASKPFDKRRDGLI
                     PSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQMAIKDAG
                     IALEQISYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAGASEVIY
                     STLMMNNGFIAPNLNFEEPDEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNSTLIIRK"
     CDS             3526..3777
                     /NRPS_PKS="Domain: PP-binding (7-75). E-value: 3.8e-14.
                     Score: 44.9. Matches aSDomain:
                     nrpspksdomains_ctg454_4_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg454_4"
                     /sec_met_domain="PP-binding (E-value: 3.4e-15, bitscore:
                     44.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNEEIIEKIRTTLAEEFEVDIDVIQPDAPLMETLELDSLDLVDM
                     VVLVEKNFGFNVTGQDFAGIKTFQDFYDLVITRMQEAK"
     aSDomain        3547..3750
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg454_4_PP-binding.1"
                     /evalue="3.80E-14"
                     /label="ctg454_4_PP-binding.1"
                     /locus_tag="ctg454_4"
                     /protein_end="75"
                     /protein_start="7"
                     /score="44.9"
                     /tool="antismash"
                     /translation="EKIRTTLAEEFEVDIDVIQPDAPLMETLELDSLDLVDMVVLVEKN
                     FGFNVTGQDFAGIKTFQDFYDLV"
     CDS             3780..4541
                     /locus_tag="ctg454_5"
                     /transl_table=11
                     /translation="MSTWKGKTRGGTFGYLFFIYLIKYLGITAAYIFLSLVVLYFIPFA
                     PKATKSTWFYARHILKYNRIRSLGMLLRNYYRLGQILIDKVAIGNGKVDQYRFEFERYP
                     EFLQLLNSEQGVIMIGAHVGNWEIGVPFFDDYGKKINIVMYDAEHRRIKEILEKNGQDK
                     DFKIIPVNEDNLTHVFRITEALNKKEYVCFQGDRYLNKEKLLTGTLLGQKAPFPAGPFL
                     LGSRMKVPVVFYFAMREPGRTYRFHFIRTEPV"
ORIGIN
        1 gaaaatgata gctgacaaaa gtataaattt agataccctt cataaagtat tgtttgataa
       61 tgagaagctg gaactctctg aagaatgtat tcgaaaagta gaggaaagct tcgattttct
      121 gcaatccttt tccagcgata agattattta tggtatcaat acggggttcg gcccaatggc
      181 acaatacaga atagaagatc agtcattgat cgaccttcag tataatatca tccgaagcca
      241 ttccaccggt gccggcaaac cgcttcccga actttatgta aaagcagcta tgattgcccg
      301 tttgtacact tttctacaag ggaagtcagg agtgcatctg gaactggttt ctctcctctg
      361 tgaatttatc aaccgcggaa tttatccgtt catacccgaa cacggaagtg taggtgccag
      421 cggcgatctc gtacaactgg cccatatcgc cctgacgtta ataggggaag gggaagtttt
      481 ttatcagggt aaattgtgta acgcagctac ggtgcttcag gaaaacggcc tgaaaccttt
      541 ttccatgcgt attcgtgaag gtttatccgt tacaaacggt acttctgtaa tgacaggcat
      601 cggtattgtc aatctgattt atgcaaaaaa actactccgt tggtcggtgg ctgcctctgt
      661 aatgatgaat gagattgccg cctcttatga tgattttatg gcacaggcat taaacgaggc
      721 caagcatcat aaaggtcaac aagagatagc tgctatgatg agagaatggg tggcaggcag
      781 taaatgcgtg cttcaaagag agaacgagct atacaaccag gtgcataaag agaaaatctt
      841 cgaacacaaa gtacagccct attattcctt gcgatgtgtt ccgcaaatac tcggtcctat
      901 ttacgatgaa ctggagaatg cggaagaagt attaataaac gaaataaatt ccgcctgtga
      961 caatccgatt gtcgatccgg atacacaaaa tatttatcat ggcggcaact tccacggaga
     1021 ttacatttct ttcgaaatgg acaagttgaa aattgccgtg accaagctga ctatgctttg
     1081 cgaaagacag attaactatc tgttccacga ccgtatcaat ggcatcctgc ctccgtttgt
     1141 aaatttggga gtgcttggat tgaactatgg tttacaggct tcgcaattca ctgcaacctc
     1201 caccacagcg gagtgtcaga cattatcaaa tccgatgtat gtacacagta tccccaacaa
     1261 caatgataat caggatattg tcagcatggg aaccaactcg gctctattag caaaaacagt
     1321 cattgagaat tcttatcagg tgatggctat ccagttcatg ggaatggcac aagctatcga
     1381 ctacctgaaa atacaggatc gcctaagttc caaaagcagg caggtttatg aagaaatacg
     1441 cagtttcttc cctgtattta ccaatgacac acctaaatat aaagagatag aaatgatgat
     1501 agactatctc aaaaaagaag ataaataaaa tgaaatatgc attagtaacc ggaggaagcc
     1561 gaggtatcgg ccgtgctgtc agttgtaaac tggcggaaat gggctacttt atactgataa
     1621 attatcagaa taacgacgca gaagcggaga aaacgttgca gttagtacag gaaaaaggaa
     1681 gtaatggcga gttgatgaag ttcgatgtga ccgaccctgc cgctatcacc cttgctttgg
     1741 gcaactgggc ttcccaacac cctgacgaat atatcgaggt actgataaac aatgcaggta
     1801 tccgcaaaga caacctgatg ctctggatga caggagaaga atggagcaaa gtgctcgata
     1861 tcagcctgaa cggattcttc aacgtgacac agcctttatt gaagaatatg ctggtcaaac
     1921 gttatggacg tatcgtaaat atcgtgtcat tgtcgggcat tcagggaatg cccggccaaa
     1981 ctaattactc cgcagccaaa ggtggcgtaa tagccgctac caaagcactg gcacaggaag
     2041 ttgccaagaa gaaagtaaca gtcaatgcag tagctccggg attcatccgt acggatatga
     2101 cggaaggaat tgatgagaac gaatggaaga aacatattcc cgcgggacgt ttcggcactc
     2161 cggaagaggt tgccgacctg gtcggtttcc tggcatctcc agcctcatcc tacatcacag
     2221 gagaagtgat ttccatcaac ggaggactat atacttaaaa taaaagcata gaatagacaa
     2281 aataatgaaa agagttgtta tcacgggaat ggggatttac tcttgcatcg gcaagaatct
     2341 ggatgaagta aaagactcac tatatcatgg gaaatcggga attggcatag acccggtacg
     2401 gaaagaattg ggctattttt ctgcattaac ggggatattg gaacgtcccg acttgaagaa
     2461 gttgttggac cgccgtaaac gtctctgtct accggagcag ggagaatatg cttatctcgc
     2521 tacattagaa gctttccgta atgcagggat tgacgagaat tttctcgaat ccaacgaagt
     2581 gggtatcttg tatggcaatg atagtagcgc cgctcctgtt attaacgcag tggacatcat
     2641 tcgtgaaaag aaaaacactg cactagtggg ttccggttct attttccagt cgatgaattc
     2701 caccgtgaca atgaacctat cggttatttt caaacttaga ggggtcaact tcactattgc
     2761 cggagcctgt gcaagcggtt ctcacgctat tggtatgggt tacctgctta taaaatcggg
     2821 attacaggat tgtattcttt gcggtggcgc acaagaagtc aatccttatg cagtaggaag
     2881 tttcgacgga ctaagcgcat tttccaccca ggaagcagcc cccgagaaag cctcgaaacc
     2941 ttttgataaa cgacgtgacg gactgattcc gagtggagga gccgccagtc tggtattaga
     3001 gagttatgaa tcggctgtaa aaaggggagc tcccattctg gccgaagtca tcggttacgg
     3061 tttttcatcc aatggagatc acatctccgt gcctaatgtg gacggcccga aacgttctct
     3121 tcaaatggct atcaaagatg cagggattgc actcgaacag atcagttata tcaatgcaca
     3181 cgccacctca actccggtag gtgacttgaa cgaggcgaaa gccattgcgg aagtatttga
     3241 agggcatcac ccgtatgtga cttccacgaa gtctatgaca ggacatgaaa tgtggatggc
     3301 cggtgccagt gaagtgattt attccaccct catgatgaac aacggtttca tcgctcccaa
     3361 cctcaatttc gaggaaccgg acgaggcctc tgcacaatta aatataccta cccaacgggt
     3421 aaacctggaa tttgatacgt tcctttccaa ctcattcgga ttcggaggaa ccaattccac
     3481 gctaatcata cgcaaaatca atcnnnnnnn nnngaaaaca gatatatgac aaacgaagaa
     3541 atcatcgaga aaataagaac cactctggca gaagagtttg aagtagacat cgacgttatc
     3601 cagccggatg caccgttaat ggaaactctt gaactggaca gcctggactt ggtcgatatg
     3661 gtggtactgg tagaaaagaa cttcggtttc aacgtaaccg gacaagattt tgcgggtatc
     3721 aaaaccttcc aggacttcta cgatctcgtt atcacccgta tgcaggaagc caagtaaata
     3781 tgtcgacgtg gaaaggcaaa acccggggag gaacattcgg atacttattc tttatttacc
     3841 tgattaagta tctgggcatt actgccgctt atatatttct aagtctggta gtattgtact
     3901 tcataccgtt tgctccgaaa gccaccaaaa gcacctggtt ttatgcccgc cacattctga
     3961 aatacaaccg aatacgctct ttgggtatgt tgctacgcaa ctattaccgg ttagggcaaa
     4021 tcctgattga caaagtagct attggcaacg gaaaagtcga tcaataccgg ttcgagttcg
     4081 aacggtatcc ggagttcctg caattgctga acagcgaaca gggagtgatt atgattggcg
     4141 cccatgtggg aaactgggaa ataggcgttc ctttcttcga cgattacgga aagaaaatca
     4201 atatcgtgat gtatgatgcc gaacatcgta gaatcaaaga gatattggag aaaaatggtc
     4261 aggacaagga ctttaagatt atccccgtca acgaagataa cctgacacac gttttccgta
     4321 ttaccgaggc gttaaacaag aaagaatatg tatgcttcca gggcgaccgt tatctgaata
     4381 aagagaaact gctgacagga acactcttgg gacagaaagc tcctttccct gccggtcctt
     4441 tcctgcttgg ttcgcgaatg aaagttccgg tggtattcta ctttgccatg agagagccgg
     4501 gcagaaccta ccgttttcat tttataagga cggaaccagt c
//
