LOCUS       c00335_gnlPro..         6294 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LCIAKKDL_340.
ACCESSION   c00335_gnlPro..
VERSION     c00335_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 12:10:53
            Original ID  :: gnl|Prokka|LCIAKKDL_340
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6294
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6294
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4982:6293]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4983..6293
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6294
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6294
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             26..160
                     /locus_tag="ctg335_1"
                     /transl_table=11
                     /translation="MENSCNDELFCSLKNELREVTAQINVHSEKLMELLDQKKESSVN"
     CDS             169..414
                     /locus_tag="ctg335_2"
                     /transl_table=11
                     /translation="MSHLTPVTIEYRGNPKQYVSVVLDAINRGRLTYDGIANCEQTFRA
                     LASVVDVISPKNGKTLSVETLVSYEKKKRAGEFEEK"
     CDS             471..803
                     /locus_tag="ctg335_3"
                     /transl_table=11
                     /translation="MKREIIAYKGYFKEFFDRLDTGTQDKILYVLMLLQTQDRIPLKFM
                     KLIEEGLYELRIEYQSNIYRIFFCFDEGRIVILFNGFQKKTEKTPKKEIEKAKILRKEY
                     YGSKNK"
     CDS             784..1089
                     /locus_tag="ctg335_4"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTVTRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCRIEFSMSL"
     CDS             1107..1196
                     /locus_tag="ctg335_5"
                     /transl_table=11
                     /translation="MVGNLKKLTVFFKFPTVFLRKKEEEKGKR"
     CDS             complement(1217..3313)
                     /locus_tag="ctg335_6"
                     /transl_table=11
                     /translation="LQPQIKKNKPTXXXXFYRVIAEMTEEEIVSAISNGTYRETVEDVR
                     RIFAEQGEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARIL
                     ALAKKCAHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQL
                     CGVEVDTSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTA
                     PDNNPLTEQVALNYHSSHTSLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQE
                     EAAAFIKSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRMLQIEQHIKENYDTRY
                     NEVLHIMEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSI
                     TYHPIREYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNH
                     TVLLLCSEIQNIGKTTFINNLLPPELRAYLSTGLINPSNKDDLAKIAQAMLINLDEFEG
                     MSGRELNIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCF
                     HVDSMEFIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLT
                     HIRKPERFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRY
                     TXXX"
     CDS             complement(3369..3599)
                     /locus_tag="ctg335_7"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNPPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             3793..4269
                     /locus_tag="ctg335_8"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGXXXRCV"
     CDS             4358..4525
                     /locus_tag="ctg335_9"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             4512..4955
                     /locus_tag="ctg335_10"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDEDGKAADTRTSRQKESLHRLVRELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(4983..5711)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg335_11"
                     /sec_met_domain="Radical_SAM (E-value: 4.5e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5727..6293)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg335_12"
                     /sec_met_domain="Gly_radical (E-value: 3.4e-48, bitscore:
                     152.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 7.8e-18, bitscore:
                     53.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IRDSDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVM
                     YGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIV
                     PKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLNREMLYDAMEHPEKYPQLTIRVSG
                     YAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tataatcaat taaatgaaaa gttttatgga aaattcatgt aatgatgaat tgttttgttc
       61 attgaaaaat gaacttcgtg aagtaaccgc acaaatcaat gtgcatagtg aaaaattaat
      121 ggagctgctc gaccaaaaaa aagaatcgtc agtcaactga ctcgagagat gtcgcatctc
      181 acgcctgtca ccattgagta tcggggaaat ccgaagcaat atgtctctgt tgtgctcgat
      241 gccatcaatc ggggtaggtt gacgtatgac ggaatagcaa attgcgaaca gacttttcgt
      301 gcgttagcaa gcgttgtaga tgtgataagc cctaagaatg ggaaaacgct tagtgtagag
      361 acgctggtgt cttatgagaa gaaaaagcgt gccggagaat ttgaggaaaa ataatataaa
      421 ataaaaagac gaatgttgta tagttgaaca acaattcata tatttgcagt atgaaaagag
      481 aaataatagc atataaagga tatttcaaag agttctttga tcgtctggat actggcaccc
      541 aagataagat attatatgtg ttaatgctat tgcaaacgca ggacagaata cctttgaagt
      601 ttatgaagtt gatagaagaa ggactttatg aacttcgtat tgagtaccag agtaatatat
      661 atagaatatt cttttgtttc gatgaaggac gtattgtgat actctttaac ggattccaaa
      721 agaaaacaga aaagacccca aagaaagaaa ttgaaaaagc aaagatatta agaaaggagt
      781 attatggaag caaaaacaaa tgaggaattc tttaacgtca gcgcattaat agatgagcgt
      841 tttggtaagg aaggaactgt tactcgtgcg gaagcagaag aaaaagcgta tgctttctac
      901 accgggcaaa ttatcgaaga cgcaaggaaa aaggctaaga ttactcaagc agagttagcc
      961 cgacgtatcg gttccgaccg ttcttatatt tcaagagtgg aaagtggtca gacagagcct
     1021 aaggtttcta ccttctatcg catcatgaat gcgttaggtt gtaggatcga attttcgatg
     1081 tctttataag aagcgattaa aaaaacatgg tgggaaattt gaaaaagtta actgtctttt
     1141 tcaaatttcc caccgttttt ttgaggaaga aagaggaaga aaaaggaaaa aggtgagggt
     1201 aggtgagggt aaatttctan nnnnnnnnng tgtatcttct cccgtttttt cccttatgaa
     1261 attcgaagcc gtgtttggac atcacttttc ctagttgggc tttggttccg tgagaatatt
     1321 gataccccgt ccgttcgcga atcaattccg caatttcagt gaccgtcaga taatgcacct
     1381 tttcgaaacg ttccggtttg cggatatgtg tcaacaccag ttcttcttcc ggtgagtgga
     1441 aaatgaaatc ctcgttgttc tcttcaatcc gtgagttttc cgactgggtg aaccaatact
     1501 gataccccgg cttgttgagt agatacttga tttgtgcata caactgcgcg tagtttattt
     1561 tgataaactc catcgagtcg acatgaaagc agagaaaacg gcggttacct gtggtgtcgt
     1621 gaagcacttc ctggtaattg catgtgccgg caaaagaagc cgtatgagga aagttctgcg
     1681 aacgccgcgc gtatggcagg cggatgctga ttactttgcg ggtgactaaa tccttgaaga
     1741 tattcagttc ccgtccgctc atcccttcaa actcatccag attgataagc atcgcctgcg
     1801 ctattttagc cagatcatct ttgttgctcg ggttgataag accggtagaa agataggcgc
     1861 gcaattcggg cggcagcagg ttgttgataa acgtagtctt tccgatgttc tgtatttcgc
     1921 tgcaaagcaa caaaacagtg tggttcacca catcatcttg tgtggcggca gcgcacattc
     1981 ccaccagata acgttccaga cattctaccc aaaatttctg gtgactagta tgcacgctgt
     2041 tggcaaggat accgatataa tccgttccgt cccattcagg cagcgagtct aaatactcgc
     2101 gaataggatg gtaggtgatg ctgaagtcgg agtagatcag attctgaatc gtcttgacgg
     2161 tgcacgaata gcccagttcg ttcatctcca tccaaatgct gttttccatc atttcgtcga
     2221 gaatatggaa aggttccggt tgctccgtgt ccgttttcct tctgcgatat tccatgatat
     2281 gaagcacttc gttgtagcgt gtatcgtaat tttctttgat atgttgctca atctgtaaca
     2341 ttcgcttctg cgtactattc agcttgcggg tgtcgaattc ctcgttgtgt ccgtaagcac
     2401 tcccgatcag tgcgtccatt tcatccgcgg gaagatcggt gaactggctc ttgataaaag
     2461 ctgccgcctc ttcctggggg acgccgtagc gattgtacat acaagccagg caatgcaggt
     2521 agttgttccg gttgccggtg acgtactttt cggatttatt gtgataatag ttcagagtca
     2581 ccattaagga agtgtgcgag gaatggtagt tgagggccac ttgttcggtg aggggattgt
     2641 tgtctggtgc tgtcgtcttt ttctttttgc cggatgccct tttcttcttt tgagacttga
     2701 agaacatcgg tggctgttcc acgatgacag ctgtggcatc gggattgaaa taaatatccg
     2761 gatcgtaaga gaacaggcaa gtgcggctaa catcctgccc ggaagtgtcg acctcaactc
     2821 cgcagagctg ggcataaaag gaagcgattt tgtgataagc agcatgatgg aaatcttcaa
     2881 tttcctgaat tgtttcgggc aatgtcccgt ctgtccggca tacacgtacc acgattttca
     2941 gtcccattcc tttggggctg atgaaagcga tgcgggtgtg ggcacacttt tttgccagtg
     3001 cgaggatgcg tgccaatgct tcctgtgtct gatggtcgat gtctaccacg atgtatccca
     3061 gatacttgac gagagttgcg tttgaacgtc ctccccggta gttggctgaa aaggtgattt
     3121 ctggtagttc tattttcttt tcgttggctg ctttctcgcc ttgttccgcg aagattcggc
     3181 gcacgtcctc caccgtttcc cggtatgtgc cgttgctgat ggccgatacg atttcttctt
     3241 ctgtcatttc ggctataacg cggtaaaagn nnnnnnnnna tgttggtttg ttctttttaa
     3301 tttgcggttg caaagaaatt tgtttgtaaa ataatagaat ggaacttttc gtttttttga
     3361 ttaagttcct agggttctcc caaatagcgt atgatcgttg ccacttgccg gggagagaga
     3421 aggcggttgc gggcacgata gccttcttgt tcgagttctt gcaggagcgg agggttgtag
     3481 acaatccaac gacgtagtat ttgtaaggct actttaaggc atacgttggg gttgtaaaga
     3541 tgagctagtt ccgacttttt ataagtgcgg atatgaaacg gctggtccgg ataatacatg
     3601 atgaataatg tttggtttat gctataaagt tactgaaaat taatggttta ttaaaagaat
     3661 aacgtcatta ttattcatag agtatcagga aacttcgttg gggaatccgg aggatggaaa
     3721 atgtgttatc tttatgtcgt taacaaataa ggaacgcgtt cgaaatgtgt gaaatcttaa
     3781 aattatttta ttatggcaat gacagtaact tattcagtag tgccgcgcaa gaatcctgcc
     3841 aaaaaggatg agtcggcaaa gtattacgcc caggcacagg cttcgggaga gttggatttt
     3901 gaagagttgt gcgaaggaat caccagtcgg tctacctgta cggagacaga tgtgcgtgcc
     3961 gccatttcgg gtattcttta tgaggcgaag cgtgcgttga aagcgggaag aatagtccgg
     4021 ctgggtgatt tgggcagttt gcagatcggg ttgaacagtg aaggagctgt gtcggtcaag
     4081 gagttttcaa gttcgctgat tacggctgcc catattattt tccgtcccgg aaagacgttg
     4141 gcggacatca cgaagattct tagctaccag caggtaacga cgcgtgcggt ggctcaaacg
     4201 ggcggtagcg gcaatgaagg agaagacgat gacaaaggtt ccggtggnnn nnnnnnnaga
     4261 tgtgtataag agacaggggt tccggtggtg gttcagacgg tggtggaagc ggagaagctc
     4321 cggatccggc agcatgataa ctttaatatt ggatcaaatg aaagaaattg taactaagat
     4381 tttggatgta attatgttcc ttgtgccttt cttcggaaaa agaaagcgga acagaattgt
     4441 gagagaggtg cgcttcaatg ctacccataa ggaagtgtgt aatgtgaaaa cgacggaaag
     4501 ggagaaggac gatgagaaag attagtctga ttgtgattca ctgctctgct acccgtgctg
     4561 atcgcgactt cacggcgaag gatgtggaca ctgcccatcg ctttcggggc ttttcgtgtt
     4621 gggggtatca ttactacatt cgcaagtcgg gacagataga gccgatgagg gatgaagata
     4681 cggtgggtgc tcatgcacgc ggttttaatg cgattagttt aggggtgtgt tatgaaggcg
     4741 gactggatga ggacggaaag gcagcggata cgcgtacttc ccgccagaaa gaatctttgc
     4801 atcgcctggt gcgcgaactg ttgcagcgtt atccggatgc aaaagtggtg ggacaccgtg
     4861 atttgagtcc tgacaccaat tataatggaa ttgttgaccc ttgggagcgg atcaaagagt
     4921 gtccctgttt cgaggtgaaa gcagagacat ggtgattttt atcctattta ttactgtggt
     4981 ggttaattga ccaccacagt agtgaaatac tctttaaata cctccgcagc tttttcaagt
     5041 tgttcggggg tattttcttt tacgtctttc agcttgtatt cctgctccat cgcttcatac
     5101 ttatgtacgc ctaatgtatg ataaggcagg atttccactc gttgcaccat cttatatttt
     5161 cccagagctt ctcctaaacg gcggatgtct tcctcgaaat cgctgtatcc gggcactaat
     5221 acataacgca gccagaaagg tttctcgttt tcttccagcc atgctgccgt gcggatggtt
     5281 tgctcgttac ttcttccggt cagtgcttga tggcgtgccg gattgaactc tttaatatcc
     5341 agcaatacaa gatccgtcag tttgaaaagt tcttcaactt cttcgttcca gataccgccg
     5401 ttgctgtcta tgcatacatg tattcccttt tctttcagtt cacgcactaa agggactagt
     5461 gctttggcct gaaacgtagg ttctcctccg gagaaagtga ctcctccgcg tttcccgaaa
     5521 aaaggacgtt ggctcatagc catgcggacg atttcttccg gtggagtagg tgtgcctcct
     5581 ttcccggcta ttgtgtcagg gttggcgcaa tacaggcagc ggaaattgca tccttgaaga
     5641 aaaacgacga gccgtaagcc cggcccgtcg aatgttccca tactttcgta tgaatgtacg
     5701 tttatcgtca tattgagtag atctgattac atacgttcgt ggaagctacg gctgataact
     5761 tccaattgat gttcgcggct caacttcacg aagtttacgg cataaccgga aacacggatg
     5821 gtgagttgcg gatatttttc cggatgttcc atggcatcgt aaagcatttc acggttcagt
     5881 acgttcacgt tcaggtggtg agcgcctttg gtgaaatagc catccatcat tgtcaccaga
     5941 ttttctacgc gatcctcttc agtagctccc agtgatttgg ggacgatgga gaaagtgttg
     6001 ctgataccgt cctgtgagtc gcggtaacgt aatttcgcta cggaactcaa agaggcgatg
     6061 gcaccgttct tgtcacgtcc gtgcataggg ttggctcccg gagcaaaagc gacacctttg
     6121 gcgcgtccgt cgggagtagc acctgtcttc ttgccataca tcacgttgga agtgatggtg
     6181 agcaaagata aagtaggacg ggcatttttg taaaccggaa gtttcttcaa ttcttcgctg
     6241 aagaaataga ccaggtcaac acccaggtgg tctaccttgt cgctgtctct tata
//
