GCA_024653545.1 - 16 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaLNBFJIIK_1 (original name was: gnl|Prokka|LNBFJIIK_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	other	120,672	162,090	bacilysin	Other	100%

gnlProkkaLNBFJIIK_4 (original name was: gnl|Prokka|LNBFJIIK_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	PKS-like	1	27,293	butirosin A/butirosin B	Saccharide	7%
Region 4.2	terpene	110,091	130,831

gnlProkkaLNBFJIIK_5 (original name was: gnl|Prokka|LNBFJIIK_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	transAT-PKS	99,700	205,870	difficidin	Polyketide	100%
Region 5.2	T3PKS	321,372	362,616

gnlProkkaLNBFJIIK_6 (original name was: gnl|Prokka|LNBFJIIK_6)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	transAT-PKS	1,517	89,747	macrolactin H	Polyketide	100%

gnlProkkaLNBFJIIK_7 (original name was: gnl|Prokka|LNBFJIIK_7)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS,transAT-PKS,betalactone	81,825	169,430	fengycin	NRP	80%

gnlProkkaLNBFJIIK_8 (original name was: gnl|Prokka|LNBFJIIK_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	RiPP-like,NRP-metallophore,NRPS	22,627	74,418	bacillibactin	NRP	100%

gnlProkkaLNBFJIIK_12 (original name was: gnl|Prokka|LNBFJIIK_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS	72,189	97,618	surfactin	NRP:Lipopeptide	47%

gnlProkkaLNBFJIIK_14 (original name was: gnl|Prokka|LNBFJIIK_14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	transAT-PKS,NRPS,T3PKS	1	77,339	bacillaene	Polyketide+NRP	92%

gnlProkkaLNBFJIIK_16 (original name was: gnl|Prokka|LNBFJIIK_16)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	terpene	3,140	25,023
Region 16.2	NRPS	52,009	73,764	plipastatin	NRP	30%

gnlProkkaLNBFJIIK_19 (original name was: gnl|Prokka|LNBFJIIK_19)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	lanthipeptide-class-ii	1	18,430	loseolamycin A1/loseolamycin A2	Polyketide	8%

gnlProkkaLNBFJIIK_22 (original name was: gnl|Prokka|LNBFJIIK_22)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	NRPS	12,716	39,749	surfactin	NRP:Lipopeptide	47%

gnlProkkaLNBFJIIK_25 (original name was: gnl|Prokka|LNBFJIIK_25)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	NRPS	1	13,110	fengycin	NRP	20%

gnlProkkaLNBFJIIK_26 (original name was: gnl|Prokka|LNBFJIIK_26)

Region	Type	From	To	Most similar known cluster		Similarity
Region 26.1	NRPS	1	10,767	surfactin	NRP:Lipopeptide	8%

Compact view

gnlProkkaLNBFJIIK_1 - Region 1 - other

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Location: 120,672 - 162,090 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaLNBFJIIK_4 - Region 1 - PKS-like

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Location: 1 - 27,293 nt. (total: 27,293 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)

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gnlProkkaLNBFJIIK_4 - Region 2 - terpene

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Location: 110,091 - 130,831 nt. (total: 20,741 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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No matches found.

gnlProkkaLNBFJIIK_5 - Region 1 - transAT-PKS

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Location: 99,700 - 205,870 nt. (total: 106,171 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 99699 - 205870:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal - mal) + (ohmal - mal) + (mal) + (ccmal - ccmal - ohmal - ccmal) + (ohmal - mal) + (Me-ccmal) + (mal)
Non-colinear ordering:: ctg5_144 + ctg5_145 + ctg5_146 + ctg5_147 + ctg5_148 + ctg5_149 + ctg5_150

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg5_139

PKS_AT (4..281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.7
inactive: 30.9
Methoxymalonyl-CoA: 29.3
Methylmalonyl-CoA: 21.7
Propionyl-CoA: 16.5
2-Methylbutyryl-CoA: 15.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg5_142

AMP-binding (15..363): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IGNPTGTIFK
Nearest Stachelhaus code(s):: 2,3-dohHda IGNPTGTIMK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaLNBFJIIK_5 - Region 2 - T3PKS

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Location: 321,372 - 362,616 nt. (total: 41,245 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaLNBFJIIK_6 - Region 1 - transAT-PKS

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Location: 1,517 - 89,747 nt. (total: 88,231 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 1516 - 89747:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal) + (ccmal) + (ccmal - mal) + (ccmal - mal) + (ohmal) + (ohmal - mal)
Non-colinear ordering:: ctg6_24 + ctg6_25 + ctg6_26 + ctg6_27 + ctg6_28 + ctg6_29 + ctg6_30

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg6_23

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 91.9
inactive: 31.2
Methoxymalonyl-CoA: 26.1
Methylmalonyl-CoA: 19.6
Propionyl-CoA: 16.9
fatty_acid: 13.9
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaLNBFJIIK_7 - Region 1 - NRPS,betalactone,transAT-PKS

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Location: 81,825 - 169,430 nt. (total: 87,606 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 81824 - 169430:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Tyr) + (Ile) + (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 81824 - 159075:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 141989 - 169430:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg7_110

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AMP-binding (267..674): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1789..2188): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg7_111

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code(s):: Tyr DALSVGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1779..2174): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3286..3683): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4325..4732): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg7_112

Search NORINE for peptide: strict or relaxed

CAL_domain (36..478): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 623.2
NH2: 18.6
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2956..3351): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg7_113

PKS_AT (6..290): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.7
inactive: 30.0
Methoxymalonyl-CoA: 20.4
Propionyl-CoA: 11.8
Methylmalonyl-CoA: 11.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg7_132

AMP-binding (12..445): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -GLHIAAPVK
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (56% 8Å match); Ile DGLFIGIPVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg7_136

Search NORINE for peptide: strict or relaxed

AMP-binding (473..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code(s):: Ile DAFFYGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg7_137

Search NORINE for peptide: strict or relaxed

AMP-binding (146..538): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code(s):: Tyr DGTITAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaLNBFJIIK_8 - Region 1 - NRP-metallophore,NRPS,RiPP-like

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Location: 22,627 - 74,418 nt. (total: 51,792 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 22626 - 74418:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 22682 - 74418:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg8_51

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (100% 8Å match); diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg8_53

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaLNBFJIIK_12 - Region 1 - NRPS

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Location: 72,189 - 97,618 nt. (total: 25,430 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 72188 - 97618:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg12_91

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code(s):: Leu DAFFLGCVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaLNBFJIIK_14 - Region 1 - NRPS,T3PKS,transAT-PKS

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Location: 1 - 77,339 nt. (total: 77,339 nt) Show pHMM detection rules used

Region on contig edge.

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T3PKS: cds(Condensation and (AMP-binding or A-OX))

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 77339:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg14_5

Search NORINE for peptide: strict or relaxed

AMP-binding (492..896): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNMAIIYK
Nearest Stachelhaus code(s):: Ala DVSNMAIIYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg14_8

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 26.7
NH2: 9.4
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg14_13

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 89.6
inactive: 28.2
Methoxymalonyl-CoA: 21.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg14_14

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 31.9
inactive: 26.1
Methoxymalonyl-CoA: 16.2
Benzoyl-CoA: 14.0
Methylmalonyl-CoA: 9.1
Isobutyryl-CoA: 8.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg14_15

PKS_AT (8..289): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 100.0
inactive: 46.8
Methoxymalonyl-CoA: 38.9
Methylmalonyl-CoA: 25.3
Propionyl-CoA: 23.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaLNBFJIIK_16 - Region 1 - terpene

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Location: 3,140 - 25,023 nt. (total: 21,884 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

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regulatory genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaLNBFJIIK_16 - Region 2 - NRPS

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Location: 52,009 - 73,764 nt. (total: 21,756 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

gnlProkkaLNBFJIIK_19 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 18,430 nt. (total: 18,430 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

Lanthipeptide predictions

Lanthipeptide(s) for ctg19_5

ctg19_4

Leader:

MSEKQIERTIRAWKDPEFRKTLSDVADHPSGSIEARQLSMLGS

Core:

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

Core with dehydrations:

EENVNPMDhbDhbPVDhaPAPDhbVVIRVDhaLKVCysPDhbFKIKCysKV

MIBiG 3.1 matches for ctg19_4

14.3% BGC0000553 (SSGG_RS09925): Lanthipeptide: SRO15-2212

20

RVSLKVC

26

+VSL VC

4

QVSLLVC

10

antiSMASH-DB 4.0 matches for ctg19_4

100.0% NZ_CP039297.1and 2 others:

NZ_CP040378.1 Lanthipeptides: D1120_RS00435_lanthipeptideNZ_CP059318.1 Lanthipeptides: HZT45_RS00565_lanthipeptide

Lanthipeptides: E4T61_RS00570_lanthipeptide

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

97.1% NZ_CP040672.1and 1 other:

NZ_CP087137.1 Lanthipeptides: LOK79_RS00585_lanthipeptide

Lanthipeptides: FGE23_RS05605_lanthipeptide

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

E+NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

1

EDNVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

42.9% NC_018499.1and 5 others:

NC_018499.1 Lanthipeptides: BCK_RS27840_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27835_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27845_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27840_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27835_lanthipeptide

Lanthipeptides: BCK_RS27845_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+VNP TTP +P ++ VSL +CPT K K

2

DVNPETTPATP---TIVAVSLGICPTTKCTSK

30

with 43 more sequence matches

42.9% NZ_CP023481.1and 2 others:

NZ_JALARN010000006.1 Lanthipeptides: MOE03_RS17410_lanthipeptideNZ_JAQCPU010000013.1 Lanthipeptides: PG077_RS20425_lanthipeptide

Lanthipeptides: COP00_RS24795_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +RV S+K CP+FK KC

1

NDEITPQSTVVCVSLRVCKWSIKYCPSFKRKC

32

42.9% NZ_PIJH01000018.1: Lanthipeptides: CV632_RS18055_lanthipeptide

9

TPVSPAPTVV---IRV---SLKVCPTFKIKC

33

T VSP T+V +R+ SL+ CP+FK+KC

2

TEVSPQSTIVCVSLRICNWSLRFCPSFKVKC

32

40.0% NC_009328.1: Lanthipeptides: GTNG_RS18990_lanthipeptide

9

TPVSPAPTVV---IRV---SLKVCPTFKIKC

33

T VSP T+V +R+ SL+ CP+FK++C

2

TEVSPQSTIVCVSLRICNWSLRFCPSFKVRC

32

40.0% NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09365_lanthipeptide

Lanthipeptides: AOX59_RS09365_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFK

30

+V+P TTP S A I VS CPT K

2

DVDPQTTPSSVA--CGIAVSALFCPTTK

27

40.0% NZ_CP015591.1and 15 others:

NZ_CP078085.1 Lanthipeptides: KU891_RS29355_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29350_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29345_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29340_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29335_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29930_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29925_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29920_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29910_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29930_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29925_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29920_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29910_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23050_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23050_lanthipeptide

Lanthipeptides: A7A70_RS27550_lanthipeptide

3

NVNPMTTPVSPAPTVV---IRVSLKVCPTFK

30

+VNP TTP + PT+V I ++ +CPT K

2

DVNPETTPAT--PTIVEATIAATVSICPTTK

30

40.0% NZ_CP036019.1and 1 other:

NZ_CP036019.1 Lanthipeptides: EXW52_RS27205_lanthipeptide

Lanthipeptides: EXW52_RS27205_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+V+P TTP +P T+V+ +S+ +CPT K K

2

DVSPETTPATP--TIVVAISVGLCPTTKCTSK

31

40.0% NZ_JALAJI010000006.1and 3 others:

NZ_JALAOO010000008.1 Lanthipeptides: MOC69_RS16910_lanthipeptideNZ_JALAXE010000008.1 Lanthipeptides: MOF11_RS14910_lanthipeptideNZ_JALAXI010000013.1 Lanthipeptides: MOE73_RS14185_lanthipeptide

Lanthipeptides: MOB90_RS08905_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +R+ S+K CP+FK KC

1

NDEITPQSTVVCVSLRLCKWSIKYCPSFKKKC

32

40.0% NZ_JALANL010000003.1and 3 others:

NZ_MUEI01000003.1 Lanthipeptides: BV582_RS23575_lanthipeptideNZ_NIJN01000002.1 Lanthipeptides: CHCC5027_RS22460_lanthipeptideNZ_NIMF01000037.1 Lanthipeptides: CHCC14814_RS22115_lanthipeptide

Lanthipeptides: MOC63_RS03255_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +R+ S+K CP+FK KC

1

NDEITPQSTVVCVSLRICKWSIKYCPSFKRKC

32

40.0% NZ_NUML01000015.1: Lanthipeptides: COD66_RS20140_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+V+P TTP +P T+V+ +S+ +CPT K K

2

DVSPETTPATP--TIVVGISVGLCPTTKCTSK

31

38.5% NZ_CP009280.1and 1 other:

NZ_CP009280.1 Lanthipeptides: P40081_RS37160_lanthipeptide

Lanthipeptides: P40081_RS37160_lanthipeptide

3

NVNPMTTPV--SPAPTVV--IRVSLKVCPTFKIKCK

34

+VNP TTP+ S P V+ + +S +CPT K K

3

DVNPETTPLTTSSWPCVIGAVDISANLCPTTKCTSK

38

37.1% NZ_CM000721.1and 9 others:

NZ_CP015591.1 Lanthipeptides: A7A70_RS27555_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29360_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29940_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29935_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29940_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29935_lanthipeptideNZ_NUBO01000052.1 Lanthipeptides: CN575_RS29785_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23040_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23040_lanthipeptide

Lanthipeptides: BCERE0010_RS29355_lanthipeptide

3

NVNPMTTPVSPA-PTVVIRVSLKVCPTFK

30

+VNP TTP +P I V+ +CPT K

2

DVNPETTPATPTIIGATIAVTASLCPTTK

30

37.1% NZ_CP036019.1and 7 others:

NZ_CP036019.1 Lanthipeptides: EXW52_RS27180_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27175_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27170_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27190_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27180_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27175_lanthipeptideNZ_CP036019.1 Lanthipeptides: EXW52_RS27170_lanthipeptide

Lanthipeptides: EXW52_RS27190_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+V+P TTP +P T+V+ +S+ +CPT + K

2

DVSPETTPATP--TIVVAISVGLCPTNRCTSK

31

37.1% NZ_FOHE01000009.1: Lanthipeptides: BMX40_RS20255_lanthipeptide

2

ENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKC

33

E V P +T V + + + S+K CP+FK KC

2

EEVTPQSTVVCVSLRLC-KWSIKYCPSFKRKC

32

37.1% NZ_MLKL01000042.1: Lanthipeptides: BKK45_RS27405_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPT

28

+VNP TTP +P ++ VS+K+CPT

2

DVNPETTPATP---TIVAVSVKLCPT

24

34.3% NZ_CACZYW010000004.1and 3 others:

NZ_CACZYW010000004.1 Lanthipeptides: HUF10_RS02820_lanthipeptideNZ_FNHR01000005.1 Lanthipeptides: BLQ37_RS05585_lanthipeptideNZ_FNHR01000005.1 Lanthipeptides: BLQ37_RS05585_lanthipeptide

Lanthipeptides: HUF10_RS02820_lanthipeptide

8

TTPVSPAPTVVIRVSL----KVCPT

28

TTP +PA V++ VS K CPT

1

TTPATPATPVIVAVSAYVSDKTCPT

25

34.3% NZ_CP036019.1and 1 other:

NZ_CP036019.1 Lanthipeptides: EXW52_RS27195_lanthipeptide

Lanthipeptides: EXW52_RS27195_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPT

28

+V+P TTP +P T+V+ +S+ +CPT

2

DVSPETTPATP--TIVVAISVGLCPT

25

34.3% NZ_JAMDLZ010000027.1: Lanthipeptides: M5W82_RS15520_lanthipeptide

2

ENVNPMTTPVSPAPTVVIRVSLKVCPTFK

30

+ P TP+ TV I +SL CPT K

1

SEIQPKWTPL----TVTIPISLAGCPTTK

25

34.3% NZ_JAQQWR010000009.1: Lanthipeptides: PQ473_RS20190_lanthipeptide

2

ENVNPMTTPVSPAPTV--VIRVSLKVC

26

+VNP TTP+ A +V I S+K C

1

SDVNPETTPLCVAYSVGATIVFSVKHC

27

34.2% NC_006322.1and 7 others:

NC_006322.1 Lanthipeptides: BLI_RS20290_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptide

Lanthipeptides: BLI_RS20290_lanthipeptide

2

ENVNPMTTPVSPAP----TVVIRVSLKVCPTFK

30

+VNP TTP + + T + VS +CPT K

1

NDVNPETTPATTSSWTCITAGVTVSASLCPTTK

33

31.4% NZ_JAHHYI010000012.1: Lanthipeptides: KM925_RS25405_lanthipeptide

3

NVNPMTTP--VSPAPTVVIRVSLKVC

26

+V P TTP V TV I +S +C

2

DVQPETTPACVGAGITVGIAISKLLC

27

30.8% NZ_CP009288.1and 3 others:

NZ_CP009288.1 Lanthipeptides: PDUR_RS29295_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptide

Lanthipeptides: PDUR_RS29295_lanthipeptide

3

NVNPMTTPVSPAP----TVVIRVSLKVCPTFK

30

+VNP TT ++ + T + VS +CPT K

3

DVNPETTILTTSSWTCITAGVTVSASLCPTTK

34

28.6% NZ_WIXN01000006.1and 1 other:

NZ_WIXN01000006.1 Lanthipeptides: GHD25_RS06900_lanthipeptide

Lanthipeptides: GHD25_RS06900_lanthipeptide

15

PTVVIRVSLKVCPT

28

PT +I VSL VCPT

9

PTTIIPVSLAVCPT

22

25.7% NZ_BOSN01000007.1and 5 others:

NZ_BOSN01000007.1 Lanthipeptides: KJS43_RS18000_lanthipeptideNZ_JAHHXV010000040.1 Lanthipeptides: KM914_RS20955_lanthipeptideNZ_JAHHXV010000040.1 Lanthipeptides: KM914_RS20955_lanthipeptideNZ_LT745762.1 Lanthipeptides: B2C77_RS21505_lanthipeptideNZ_LT745762.1 Lanthipeptides: B2C77_RS21505_lanthipeptide

Lanthipeptides: KJS43_RS18000_lanthipeptide

15

PTVVIRVSLKVCPTFK

30

P +I VSL +CPT K

8

PATIIPVSLAICPTTK

23

25.7% NZ_CP028922.1: Lanthipeptides: allorf_4058819_4059013_lanthipeptide

7

MTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

+ TP S + K CPT+ KC +

7

ICTPGSCDGDRCWLTTFKGCPTYGPKCDI

35

25.7% NZ_CP067069.1and 13 others:

NZ_CP085413.1 Lanthipeptides: LIS80_RS25350_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26960_lanthipeptideNZ_JH792240.1 Lanthipeptides: IIK_RS26240_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23985_lanthipeptideNZ_NTUF01000024.1 Lanthipeptides: CN379_RS26680_lanthipeptideNZ_NVCZ01000001.1 Lanthipeptides: COK75_RS01180_lanthipeptideNZ_NVDK01000008.1 Lanthipeptides: COK95_RS05665_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00010_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08190_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20675_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19560_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14435_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15685_lanthipeptide

Lanthipeptides: JCR32_RS24620_lanthipeptide

2

ENVNPMTTPVSPAPTVVIRVSLKVC

26

+V P TTP+ + I S+K+C

1

SDVQPETTPLCVGVIIGITASIKIC

25

25.7% NZ_JACSMH010000008.1and 2 others:

NZ_JACSMX010000006.1 Lanthipeptides: KNQ03_RS15615_lanthipeptideNZ_JACSMZ010000005.1 Lanthipeptides: KNQ06_RS16025_lanthipeptide

Lanthipeptides: KNP89_RS14470_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVC

26

+V P TTP V + +SL C

2

DVQPETTPFCVGVIVGVTISLSKC

25

25.7% NZ_JACYTN010000030.1: Lanthipeptides: allorf_08178_08354_lanthipeptide

7

MTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

+ TP S + + K CPT+ KC +

7

ICTPGSCSGDSCWLTTFKGCPTYGPKCDI

35

25.7% NZ_LDKE01000067.1: Lanthipeptides: ABB04_RS19960_lanthipeptide

2

ENVNPMTTPVSPAPTVVIRVSLKVC

26

+V P TTP+ + I S+K+C

1

SDVKPETTPLCVGVIIGITASIKIC

25

25.7% NZ_NIST01000001.1: Lanthipeptides: CD001_RS09870_lanthipeptide

5

NPMTTPVSPAPTVVIRVSLKVCPT

28

+ TP T+ RVSL C T

2

GGICTPSCLTGTLNCRVSLTFCRT

25

23.8% NZ_CP010976.1: Lanthipeptides: UB51_RS20985_lanthipeptide

3

NVNPMTTPVSPAPTVVI

19

+VNP TTP + PT+VI

2

DVNPETTPAT--PTIVI

16

22.9% NZ_CP016020.1and 34 others:

NZ_CP067069.1 Lanthipeptides: JCR32_RS24625_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25380_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25375_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25370_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25365_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25360_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25355_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19940_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19935_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23995_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23990_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00005_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08185_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08180_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08175_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20670_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20665_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20660_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20655_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20650_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20645_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19555_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19550_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19545_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19540_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19535_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19530_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14445_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14440_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15680_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15675_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15670_lanthipeptide

Lanthipeptides: A9C19_RS17970_lanthipeptide

2

ENVNPMTTPVSPAPTVVIRVSLKVC

26

+V P TTP+ + + S+K+C

1

SDVQPETTPLCVGVIIGLTTSIKIC

25

22.9% NZ_CP036019.1and 1 other:

NZ_CP036019.1 Lanthipeptides: EXW52_RS27165_lanthipeptide

Lanthipeptides: EXW52_RS27165_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTF

29

+VNP T P + P ++ ++ ++CP+

2

DVNPETIPATATP-ILANLTKELCPSI

27

22.9% NZ_CP063142.1and 1 other:

NZ_CP063142.1 Lanthipeptides: HPB58_RS21460_lanthipeptide

Lanthipeptides: HPB58_RS21460_lanthipeptide

16

TVVIRVSLKVCPT

28

TV I +SL CPT

8

TVTIPISLAACPT

20

22.9% NZ_CP063142.1and 5 others:

NZ_CP063142.1 Lanthipeptides: HPB58_RS21455_lanthipeptideNZ_JALAMS010000001.1 Lanthipeptides: MOC86_RS01475_lanthipeptideNZ_JALAMS010000001.1 Lanthipeptides: MOC86_RS01475_lanthipeptideNZ_LWAI01000057.1 Lanthipeptides: A4U60_RS22255_lanthipeptideNZ_LWAI01000057.1 Lanthipeptides: A4U60_RS22255_lanthipeptide

Lanthipeptides: HPB58_RS21455_lanthipeptide

16

TVVIRVSLKVCPTFK

30

TV I +SL CPT +

8

TVTIPISLAACPTTQ

22

22.9% NZ_CP101913.1and 1 other:

NZ_CP101913.1 Lanthipeptides: NP440_RS09140_lanthipeptide

Lanthipeptides: NP440_RS09140_lanthipeptide

15

PTVVIRVSLKVCPTFK

30

PTV I +S +CPT +

8

PTVTIPISAAICPTTQ

23

22.9% NZ_KE384107.1: Lanthipeptides: G586_RS26715_lanthipeptide

11

VSPAPTVVIRVSLKVCPTFK

30

V+P ++I S CPT K

6

VAPVVPIIIAASTAFCPTIK

25

22.9% NZ_KQ040792.1and 1 other:

NZ_KQ040792.1 Lanthipeptides: WH45_RS23705_lanthipeptide

Lanthipeptides: WH45_RS23705_lanthipeptide

15

PTVVIRVSLKVCPTFK

30

P +I VSL +CPT +

9

PATIIPVSLAICPTTR

24

22.9% NZ_LDFC01000091.1: Lanthipeptides: ABE61_RS23570_lanthipeptide

13

PAPTVVIRVSLKVC

26

P PTVV VS+ +C

6

PPPTVVSSVSIWIC

19

22.9% NZ_LT985747.1: Lanthipeptides: C8268_RS22865_lanthipeptide

7

MTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

+ TP S + + + + CPT+ KC +

7

ICTPGSCSGNNCVLTTWRGCPTYGPKCDI

35

22.9% NZ_NUML01000015.1: Lanthipeptides: COD66_RS20280_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTF

29

++NP T P +P P +++ ++ ++CP+

2

DMNPETIPATPTP-ILVNLTKELCPSI

27

20.0% NZ_BQME02000001.1and 5 others:

NZ_BQME02000001.1 Lanthipeptides: MFF31_RS04080_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04090_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04090_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04085_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04085_lanthipeptide

Lanthipeptides: MFF31_RS04080_lanthipeptide

16

TVVIRVSLKVCPTFK

30

T++I +SL CPT +

6

TIIIPISLAACPTTR

20

18.9% NZ_CM001792.1and 1 other:

NZ_CM001792.1 Lanthipeptides: C794_RS17975_lanthipeptide

Lanthipeptides: C794_RS17975_lanthipeptide

9

TPVSPAPTVVIRVSLKVCPT

28

+P++P+ + +SL CPT

8

SPLTPSSGSCVAISLAFCPT

27

17.1% NZ_MJEJ02000054.1and 3 others:

NZ_MJEJ02000054.1 Lanthipeptides: BH683_RS28810_lanthipeptideNZ_QJSP01000005.1 Lanthipeptides: DFR67_RS30760_lanthipeptideNZ_QJSP01000005.1 Lanthipeptides: DFR67_RS30760_lanthipeptide

Lanthipeptides: BH683_RS28810_lanthipeptide

21

VSLKVCPTFK

30

+S+ +CPTFK

12

LSVALCPTFK

21

allorf_00083_00283

Leader:

MSCLFCVFLHTLYNM

Core:

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

Core with dehydrations:

RNVDhbICysLFAKAGFMLVQDhaGNNRDRAIQLKNFAKHLIVRLKDhaNIVKVNEGGG

antiSMASH-DB 4.0 matches for allorf_00083_00283

100.0% NZ_CP059318.1: Lanthipeptides: allorf_0101959_0102159_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

98.0% NZ_CP039297.1: Lanthipeptides: allorf_0102024_0102224_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVN+GGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

96.1% NZ_CP040378.1: Lanthipeptides: allorf_0072224_0072424_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKH IVRLKSNIVKVN+GGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHFIVRLKSNIVKVNKGGG

51

with 4 more sequence matches

96.1% NZ_CP040672.1: Lanthipeptides: allorf_1096958_1097158_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAK LIVRLKSNIVKVN+GGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKRLIVRLKSNIVKVNKGGG

51

15.7% NZ_CP011130.1: Lanthipeptides: allorf_4095947_4096132_lanthipeptide

9

AKAGFMLVQSGNNRDR

24

AKA + +SG +RDR

9

AKATWPTCRSGASRDR

24

15.7% NZ_CP023465.1: Lanthipeptides: allorf_4137774_4138001_lanthipeptide

9

AKAGFMLVQSGNNRDRAI

26

A+A + +SG +RDR I

8

AEATWPTCRSGASRDRDI

25

11.3% NZ_VITV01000003.1: Lanthipeptides: FBZ87_RS11200_lanthipeptide

6

CLFAKAGFMLV

16

C AKAGFM++

49

CDSAKAGFMVI

59

allorf_00984_01184

Leader:

MPSYMQVSAYS

Core:

FWNFPGMHRIPAVNFSQDRRNLAVERIVTFRTAETPCFSQLLKRHTAFLTSCALL

Core with dehydrations:

FWNFPGMHRIPAVNFDhaQDRRNLAVERIVDhbFRDhbAEDhbPCysFDhaQLLKRHDhbAFLDhbDhaCysALL

antiSMASH-DB 4.0 matches for allorf_00984_01184

10.9% NZ_CP072597.1: Lanthipeptides: J5226_RS01600_lanthipeptide

30

FRTAETPCFSQLLKRHTAFLTSCALL

55

F A+ ++++ R+T+ T C +

20

FAEADIAGDNEIISRNTSACTGCGTI

45

10.9% NZ_CP101655.1and 1 other:

NZ_JAHHZH010000008.1 Lanthipeptides: allorf_106741_106917_lanthipeptide

Lanthipeptides: allorf_2707176_2707352_lanthipeptide

1

FWNFPGMHRIPAVNFS

16

FWN G H+I +

29

FWNTRGKHKIGMTGLT

44

9.1% NZ_CP035501.1: Lassopeptides: ETR14_RS17865_lassopeptide

3

NFPGMHRIPA

12

+F G+ R+PA

8

DFEGLQRVPA

17

allorf_01010_01210

Leader:

MRYDVSAAEKNRAFRLCGMLRYVLQPNYADLAKSSQREYGA

Core:

CRENSKTNRRKPACKTANGNVKKRT

Core with dehydrations:

CysRENDhaKDhbNRRKPACysKDhbANGNVKKRDhb

antiSMASH-DB 4.0 matches for allorf_01010_01210

24.0% NZ_JAAOYX010000002.1: Lanthipeptides: allorf_534411_534545_lanthipeptide

10

RKPACKTANGN

20

AC TANG+

10

SHSACSTANGD

20

21.6% NZ_BNCF01000005.1: Lanthipeptides: allorf_096655_096867_lanthipeptide

8

NRRKPACKTANGNVKKRT

25

+RRKPA + ++ V +RT

1

DRRKPASRPSSDVVARRT

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg19_4 - Class II

Cleavage pHMM score: -3.30

RODEO score: 0

Monoisotopic mass: 3716.1 Da

Molecular weight: 3718.5 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3736.5 Da N = 1; 3754.5 Da N = 2; 3772.5 Da N = 3; 3790.6 Da N = 4

allorf_00083_00283 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 5583.1 Da

Molecular weight: 5586.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5604.6 Da N = 1; 5622.6 Da N = 2

allorf_00984_01184 - Class II

Cleavage pHMM score: -6.90

RODEO score: 0

Monoisotopic mass: 6261.3 Da

Molecular weight: 6265.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 6283.2 Da N = 1; 6301.3 Da N = 2; 6319.3 Da N = 3; 6337.3 Da N = 4; 6355.3 Da N = 5; 6373.3 Da N = 6

allorf_01010_01210 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 2787.5 Da

Molecular weight: 2789.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2807.2 Da N = 1; 2825.2 Da N = 2

gnlProkkaLNBFJIIK_22 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 12,716 - 39,749 nt. (total: 27,034 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 12715 - 39749:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg22_30

Search NORINE for peptide: strict or relaxed

AMP-binding (158..560): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1204..1601): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code(s):: Leu DAFMMGMVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaLNBFJIIK_25 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 13,110 nt. (total: 13,110 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 13110:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Orn) + (Tyr - D-Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg25_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTLTAEVAK
Nearest Stachelhaus code(s):: Tyr DGTLTAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1509..1905): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg25_3

Search NORINE for peptide: strict or relaxed

AMP-binding (147..552): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEVGSIDK
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaLNBFJIIK_26 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 10,767 nt. (total: 10,767 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 10767:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - Asp - D-Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg26_2

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1502..1904): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2547..2936): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)