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Overview
1.1
1.2

### Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaLIIIEPEI_1 (original name was: gnl|Prokka|LIIIEPEI_1)

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | hglE-KS | 224,605 | 267,133 |
Region 1.2 | lassopeptide,lanthipeptide-class-iii | 1,828,313 | 1,859,587 |

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | hglE-KS | 224,605 | 267,133 |
Region 1.2 | lassopeptide,lanthipeptide-class-iii | 1,828,313 | 1,859,587 |

No secondary metabolite regions were found in these records:
  gnlProkkaLIIIEPEI_2 (original name was: gnl|Prokka|LIIIEPEI_2)

Compact view
gnlProkkaLIIIEPEI_1 - Region 1 - hglE-KS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 224,605 - 267,133 nt. (total: 42,529 nt) Show pHMM detection rules used
hglE-KS: (hglE or hglD)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 224604 - 267133:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (mal)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_182: mal
  PKS_AT (38..333): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 54.2%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 53.5
    Methoxymalonyl-CoA: 42.7
    Methylmalonyl-CoA: 21.4
    inactive: 13.9
    2-Methylbutyryl-CoA: 13.9
    Propionyl-CoA: 11.0
    Isobutyryl-CoA: 10.1
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0

gnlProkkaLIIIEPEI_1 - Region 2 - lanthipeptide-class-iii,lassopeptide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,828,313 - 1,859,587 nt. (total: 31,275 nt) Show pHMM detection rules used
lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)
lanthipeptide-class-iii: (micKC and not PKS_KS)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Lanthipeptides
Lasso peptides
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Lanthipeptide predictions
Shows the possible core peptides for each biosynthetic enzyme.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.
Lanthipeptides - No core peptides found.
Lasso peptide predictions
Lists the possible core peptides for each biosynthetic enzyme. Each core peptide shows the leader and core peptide sequences.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.

#### Lassopeptide(s) for ctg1_1550

ctg1_1550
Leader:
MKQYIAPTVETIASFREATN
Full core:
G L W F G K Y V D I G G A K A P F P W G S N
Putative macrolactam:
G L W F G K Y V D

#### MIBiG 3.1 matches for ctg1_1550

11.8% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A
12
G A K A P F P W G S N
22
G K G N
10
G C K T G A L M G C N
20

#### antiSMASH-DB 4.0 matches for ctg1_1550

100.0% NZ_CP006714.1: Lassopeptides: B2258_RS10665_lassopeptide
1
G L W F G K Y V D I G G A K A P F P W G S N
22
G L W F G K Y V D I G G A K A P F P W G S N
1
G L W F G K Y V D I G G A K A P F P W G S N
22
86.4% NZ_CP062951.1 and 1 other:
NZ_CP071248.1 Lassopeptides: BLI009_RS05010_lassopeptide
Lassopeptides: BL1347_RS03935_lassopeptide
1
G L W F G K Y V D I G G A K A P F P W G S N
22
G+W F G Y V D+G G A K A P F P W G S N
1
G V W F G N Y V D V G G A K A P F P W G S N
22
50.0% NZ_KI912489.1: Lassopeptides: allorf_513617_513742_lassopeptide
1
G L W F G K Y V D I G G A K A P F
17
G L W+G K+ D I G A K F
1
G L W Y G K F R D I F G A K T Y F
17
with 13 more sequence matches
47.8% NZ_NCVL01000001.1 and 1 other:
NZ_VMMF01000036.1 Lassopeptides: HT717_RS09510_lassopeptide
Lassopeptides: B7694_RS10650_lassopeptide
1
G L W F G K Y V D I G G A K A P F P
18
G L W F G K+ D I G +A F
1
G L W F G K F T D I F G G R A V F Q
18
40.9% NZ_KI912489.1: Lassopeptides: allorf_513868_514041_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G+W F G K+ D I G +A
1
G V W F G K W T D I F G G R A
15
39.1% NC_012468.1 and 9 others:
NZ_CGXY01000023.1 Lassopeptides: AQD87_RS12295_lassopeptide NZ_CLXO01000005.1 Lassopeptides: AFA99_RS11895_lassopeptide NZ_CMLU01000007.1 Lassopeptides: AEP96_RS12330_lassopeptide NZ_CP014326.1 Lassopeptides: AXK38_RS11095_lassopeptide NZ_CRCU01000004.1 Lassopeptides: AYW54_RS12245_lassopeptide NZ_FDQP01000002.1 Lassopeptides: A1S42_RS12075_lassopeptide NZ_FHTX02000015.1 Lassopeptides: A0693_RS10110_lassopeptide NZ_FZBS01000003.1 Lassopeptides: CFY35_RS11490_lassopeptide NZ_RXXR01000008.1 Lassopeptides: EKA98_RS05595_lassopeptide
Lassopeptides: SP70585_RS13230_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G L W G K+ D I G +A
1
G L W T G K F R D I F G G R A
15
39.1% NC_021023.1 and 26 others:
NZ_BJED01000004.1 Lassopeptides: FFR60_RS05785_lassopeptide NZ_CABGPX010000005.1 Lassopeptides: FN189_RS04890_lassopeptide NZ_CABGTR010000001.1 Lassopeptides: FN307_RS01775_lassopeptide NZ_CABGXT010000046.1 Lassopeptides: FN373_RS15575_lassopeptide NZ_CP045045.1 Lassopeptides: F9293_RS05110_lassopeptide NZ_CP046111.1 Lassopeptides: GKQ56_RS05625_lassopeptide NZ_CP063980.1 Lassopeptides: ISN59_RS06410_lassopeptide NZ_CP097069.1 Lassopeptides: M2912_RS09265_lassopeptide NZ_CP110068.1 Lassopeptides: OLM02_RS09865_lassopeptide NZ_CP116962.1 Lassopeptides: PO880_RS04740_lassopeptide NZ_GG670372.1 Lassopeptides: EFDG_RS21040_lassopeptide NZ_GL457197.1 Lassopeptides: HMPREF9517_RS19205_lassopeptide NZ_JADOEP010000002.1 Lassopeptides: IW492_RS01515_lassopeptide NZ_JADPEP010000046.1 Lassopeptides: I4U96_RS13395_lassopeptide NZ_JTKS01000001.1 Lassopeptides: QI17_RS14045_lassopeptide NZ_JTKX01000023.1 Lassopeptides: QR19_RS15990_lassopeptide NZ_KB932331.1 Lassopeptides: Q9A_RS18260_lassopeptide NZ_KB944632.1 Lassopeptides: WMI_RS18340_lassopeptide NZ_KB944658.1 Lassopeptides: WMM_RS18400_lassopeptide NZ_KB944694.1 Lassopeptides: WO3_RS18855_lassopeptide NZ_KB944828.1 Lassopeptides: WOM_RS18345_lassopeptide NZ_KE351156.1 Lassopeptides: D924_RS19170_lassopeptide NZ_KE352620.1 Lassopeptides: D350_RS18085_lassopeptide NZ_NAQY01000001.1 Lassopeptides: B6A11_RS15210_lassopeptide NZ_SCHR01000003.1 Lassopeptides: EQ847_RS03410_lassopeptide NZ_SMGV01000009.1 Lassopeptides: EYY48_RS11015_lassopeptide
Lassopeptides: ENT_RS07390_lassopeptide
1
G L W F G K Y V D I G G A K A P F P
18
G L W G K+ D+ G +A F
1
G L W T G K F R D V F G G R A L F Q
18
39.1% NZ_AULE01000001.1: Lassopeptides: H509_RS27430_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G L W G K Y D+ G +A
1
G L W T G K Y T D V F G G R A
15
39.1% NZ_KI912489.1: Lassopeptides: allorf_514674_514802_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G L W G K+ D I G +A
1
G L W T G K W T D I F G G R A
15
36.4% NZ_CP053549.1 and 3 others:
NZ_JADKSM010000003.1 Lassopeptides: ITJ61_RS18070_lassopeptide NZ_PSXX01000017.1 Lassopeptides: C5E05_RS18625_lassopeptide NZ_PSXZ01000003.1 Lassopeptides: C5E07_RS02640_lassopeptide
Lassopeptides: HMI60_RS00570_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G+W F G++ D I G +A
1
G V W F G R W R D I F G G R A
15
36.4% NZ_CP106992.1: Lassopeptides: NDS46_RS23370_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G L W G K+ D+ G +A
1
G L W T G K F K D L F G G R A
15
36.4% NZ_JAMDLY010000010.1 and 4 others:
NZ_JAPDMW010000034.1 Lassopeptides: OF778_RS29475_lassopeptide NZ_JMLT01000016.1 Lassopeptides: BP95_RS33025_lassopeptide NZ_LS992241.1 Lassopeptides: PBLR_RS07315_lassopeptide NZ_SADY01000011.1 Lassopeptides: C7Y44_RS26425_lassopeptide
Lassopeptides: M5X04_RS09885_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G+W F G+Y D+ G +A
1
G I W F G R Y R D V L G G R A
15
36.4% NZ_NGKY01000001.1: Lassopeptides: A5819_RS19010_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G+W F G++ D+ G K A
1
G V W F G R W K D V F G G K A
15
34.8% NZ_FILX01000006.1 and 1 other:
NZ_JAGFVF010000023.1 Lassopeptides: allorf_10827_10955_lassopeptide
Lassopeptides: allorf_42148_42276_lassopeptide
1
G L W F G K Y V D I G G A K A
15
G L W G K+ D+ G +A
1
G L W T G K F R D V F G G R A
15
31.8% NZ_CP009452.1 and 1 other:
NZ_NISJ01000009.1 Lassopeptides: CDQ91_RS21055_lassopeptide
Lassopeptides: LH20_RS23250_lassopeptide
4
F G K Y V D I G G A K A P
16
F G V+I G G + P
3
F G P P V E I G G H E L P
15
27.3% NZ_LMUL01000017.1: Lassopeptides: allorf_410478_410597_lassopeptide
5
G K Y V D I G G A K A P F P W G
20
G +V+ K P P G
4
G D F V E T A L T K Q P I P M G
19

#### Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine
Lanthipeptides
Lasso peptides
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists the number of lanthionine bridges, possible molecular weights, and the scores for cleavage site prediction and RODEO.
No core peptides found.
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists the number of disulfide bridges, possible molecular weights, and the scores for cleavage site prediction and RODEO.

ctg1_1550 - Class II
  Cleavage pHMM score: -8.10
  RODEO score: 18
  Monoisotopic mass: 2348.2 Da
  Molecular weight: 2349.6 Da
  Number of bridges: 0