LOCUS       gnlProkkaPAMIAFPB_2    14314 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PAMIAFPB_2.
ACCESSION   gnlProkkaPAMIAFPB_2
VERSION     gnlProkkaPAMIAFPB_2
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 13:04:56
            Original ID  :: gnl|Prokka|PAMIAFPB_2
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 3089740
            Orig. end    :: 3104054
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14314
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:9314]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14314
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14314
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             393..971
                     /locus_tag="ctg2_2287"
                     /transl_table=11
                     /translation="MILRKKKSLNAGPINGTGEGVAHSKRWYVALVRMHHEKKVSEHLD
                     KMGIENFVPVQQELHQWSDRRKLVTSVLLPMMVFVHADPKERMEVLSFSTVSRYMVMRG
                     ESSPAVIPDEQMARFRFMLDYSEESISMNSSPLARGEKVRVIKGPLTGLVGELVNVDGR
                     SKIAVRLNMLGCACVDMPVGYVEPIMAAV"
     CDS             1367..3778
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Poly_export"
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 30;
                     E-value: 3.6e-09)"
                     /gene_kind="transport"
                     /locus_tag="ctg2_2288"
                     /sec_met_domain="Poly_export (E-value: 2.6e-16, bitscore:
                     57.6, seeds: 183, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIQRLFSILLFCLMLGSGSLMAQSMSDSQVLEYVKDGIRQGKEQ
                     KQLASELARKGVTKEQAMRVKQLYEQQNNVNTSQSTGTDINESRLREETKENTSDMLED
                     HPTTQDLARGDQVFGRNIFNTRNLTFEPSVNLATPSNYRLGPGDEVIIDIWGASQNTIR
                     QQISPEGTINIQKIGPVNLSGMTVSAANDYLKNALNKIYNGLNNTTDPTSDIRLTLGNI
                     RTIQINVMGEVVQPGTYALSSFSTVFHALYRAGGVSDIGSLRNVQLVRNGKNIATIDVY
                     EFIMKGNTLDDIRLQEGDVVIVPAYDVLVKISGKVKRPMRFEMKKDENLATLIKYAGGF
                     EADAYTRSLRVVRQNGEEYEVNTVKDMDYSIYTMRNGDVVTAEAILNRFTNKLEIRGAV
                     YRPGIYQLSGKLNTIRELVHEAQGLTGDAFLNRAVLYRQREDLTSEVVQIDIRSIMDGT
                     SPNLALMKNDILYIPSIHDLEDRGNVTVYGEVAQPDSYPYADNMTLEDLIIQAGGLKEA
                     ASTVRIDVSRRIKNPRSTADNDTIGQMYTFSLKDGFVIDGQPGFILQPYDEVYVRRSPG
                     YQAQQNVVIDGEILFGGNYAMTSREERLSDLVNKAGGPTNLAYLRGAKLTRVASAGEKK
                     RMGDVIRLMSRQLGEAMIDSLGIGVEDTFTVGIDLEKALTNPKGSADLVLREGDVISIP
                     KNTNTVTINGAVMVPNTVSYMKGKNVDYYLNQAGGCSDNARKSKKFIVYMNGQVTKVKG
                     SGKKQIEPGCEIIVPGKAKKKGNIANILGYATSFSSLGMMIASIANLIKK"
     CDS             3789..4937
                     /locus_tag="ctg2_2289"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVNKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVFFGKSLLASLRKELKNKEIEQDK"
     CDS             5001..5720
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_2290"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.3e-14, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             5741..7042
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 208.9;
                     E-value: 1.5e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_2291"
                     /sec_met_domain="CTP_transf_like (E-value: 7.7e-11,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 7.9e-119, bitscore:
                     394.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 8.9e-61, bitscore:
                     203.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             7059..8183
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_2292"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.2e-18, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.6e-18, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             8193..9314
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg2_2293_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_2293"
                     /sec_met_domain="Aminotran_5 (E-value: 6e-29, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        8283..9290
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg2_2293_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg2_2293_Aminotran_5.1"
                     /locus_tag="ctg2_2293"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             9311..9454
                     /locus_tag="ctg2_2294"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             9657..11042
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg2_2295"
                     /sec_met_domain="Polysacc_synt (E-value: 5e-29, bitscore:
                     99.8, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENLKQKTVSGIMWSAIERFSLQGVQFIIQIILARLLLPSDYGMI
                     GMLAIFLQVAQVFIDSGFTNALIQKKDRTEEDFATVFYFNILVAVLFYGILFFSASLIA
                     DFYNMPTLVLVIRFIALSLIINALSAIHRTKLIISVNFKTQSKISLGAAFISGVIGIWM
                     AYVGCGVWALVWQTLLNSIILTILFYCLVHWKPLHTFSKSSFERLFNFGSKLLISSLIN
                     TVYRNLYTIVIGKKFSVVELGYYTRAEQFAIFPSNNLNALVSRVAYPILSSIQDDDKCL
                     TNAYRNYIRLSSYIIFPLMVGLLVLANPIISLLLTDKWSGVVILLQILCLDWMFDHLSQ
                     INLNLLWVKGRSDLSLRLEIIKKTIAIFILFVSIPFGLEVMCWGRVIYSLIATYLNTYY
                     TNSLIDLTLKLQVKDVFPSLILSFIMGGIVFICISFFETDIVKIVIGCITGMSFYILLS
                     FLF"
     CDS             11096..12355
                     /locus_tag="ctg2_2296"
                     /transl_table=11
                     /translation="MKILKKIFQVIVKIINHCVPVDSRSLYFIPHPNCRTDKYDIINYS
                     SDNVLVLLNYLLKKDSAEYYKIYVEIYDSSRISEYQEYIYGINPNIRCTFLCACVGRFF
                     FNKVSFKDMLYAFFCFCRASKCFTATFYYDFSFKKRSQQIICLGYYTPFKDDYHMGDHS
                     YDKFRNTTCNSFDYSIATSCLSARIISIDCGISYDKFKVLGFPRNDLLISKNNCNVIKR
                     EISKFAGYDVTKYIVYTPTFRDYETVTEGNLRSILGYVDCDLLKLSKILLKFNAALILK
                     LHPLQNKTVLKKDLPKGILVFEQTYKYGLYDLLSFSDGMITDYTSTYFDFLLVNKPVIF
                     NFYDIEEYRRVRGFSFEPIEFFCAGDIVYNYNELIDAVMGLLAGKDIHAEHRRHISLLM
                     NQFQDDNSTKRICDIFLNEI"
     CDS             12364..13113
                     /locus_tag="ctg2_2297"
                     /transl_table=11
                     /translation="MNYAFVVQARLGSTRLPGKILKPFYGNQSILDLMVHKLSAISNIP
                     VIIATTNSVINEPIEKKALALGVKCFRGEENDVLKRFIDVAEYFDIQGIFRICSDNPFL
                     DVHAARQLVEIAMKSCNDYISFDIDGTPSIKTHFGFWGEFVTLDALKRVIGFTDDLLYR
                     EHVTNYIYSHPELFNIQWISGSPVVSKHHNIRLTIDTLEDFSVAQRIYRDLQEKRVEIS
                     IEVIVDYVRRHAEYIQLMKQQIKKNSK"
     CDS             13113..14153
                     /locus_tag="ctg2_2298"
                     /transl_table=11
                     /translation="MKNTYIIGEIGQNHNGSVDLAKLIIELISRPVHEDVFGMDFMPMN
                     AVKMTKRDLSEELAVSQMNQIYDNPNSFGRTYGEHRAFLELDDQAHFEIYKYAKSLGLD
                     FIETLCAKGCLSLLKLFTPDRLKVASRDLTNLPLLEALAETHIPIILSTGMAGQRELDN
                     ALDVITRYHSNIAILHCVSQYPTHPNNLNLRTITYLKKHYNKFEIGFSDHTIGISAATA
                     AVAMGAEIIEKHVTIDRHMKGTDQLGSLGPDGVNRMIRDIRIAECWLGTEDLYIEKGVE
                     KSKIKLERSIATRKYIPAGSIIQESDIHLLSPGDGYKWIDKKNVIGHFAKQDISANEII
                     YPDFIE"
ORIGIN
        1 tgaaccggat gaatttgtct tacgtaaagc ggtgcggagt aggtcagtgt atctgattta
       61 gaagcttgat atacagtgtt tatcggtgtg cgttacttct taggtaacgg aatgattcat
      121 ggcgcaaaga tggacatatt tatgaaaata cgcaaacaaa aatgaaatta atttcttaaa
      181 atgaatcaaa agtcggcata acatacggaa aatgacaata tccgtgttat gtcttttttt
      241 tattccaatt catgaaaaac accttcactt ctgctttatt tcctgaatac cagtcctctt
      301 ctgatgtttt cgttgaaaaa agttcccttt tctgcttgcc ctatgcttgt tcgttaccta
      361 agaagtaacg gataaacatg gggtaaaaaa atatgatttt aagaaaaaag aaatcactca
      421 atgctgggcc tattaatggg acaggggaag gcgtagcaca ctcaaaacgc tggtatgttg
      481 ctttggttcg gatgcaccat gaaaagaagg tttccgagca tttggacaag atgggaatcg
      541 agaatttcgt tcccgttcaa caggaacttc accaatggag cgaccgtcgt aagctggtca
      601 cttccgtctt gcttcctatg atggtgtttg tacacgccga tcctaaggaa cgcatggaag
      661 tcctgagttt ttctactgtc agtcgttaca tggtgatgcg cggtgagagt agtcctgctg
      721 taattcctga cgagcagatg gctcgtttcc gtttcatgct tgattattcg gaagaatcga
      781 taagcatgaa cagttctcct ttggcccgtg gcgaaaaggt tcgcgtgatt aaaggtccgt
      841 tgacgggtct tgttggcgaa ctggttaatg tggatggcag aagtaagatt gctgttcgct
      901 tgaatatgct gggatgcgcc tgtgttgata tgcctgtcgg ttatgtagag ccgattatgg
      961 cggctgttta aggcaaggga tatggatgct attattttac aagaaaacat agagggttta
     1021 ttaagtttgg tacgtatgct gcttcctggt ggggggagtg caggctgcgt gtatctggat
     1081 gatctttcag cattacaagg atccatccac gaaaagatta acgatttata ttctcaacgg
     1141 gggagacacc ggagcaggat gctaccctgt gtttggccat ccttcaggga tataatgtgt
     1201 cgatgtacgc caatccggaa gacgaagaaa gaaaacaggc tgttttgacc cgttctctgt
     1261 ctcttctcga tgttcttccc ccttctcttc ttaagcaaca gttatctgcc gtctgttacg
     1321 ggatgcagga actgtgtgaa atcaactgaa acttttttat ttgtcaatga aaatacaaag
     1381 actttttagc attctactct tctgtttgat gctgggaagt ggctcgctta tggctcagtc
     1441 catgagtgat tcacaagtac tggaatatgt aaaggacggt atccggcaag gcaaggaaca
     1501 gaagcagctt gcttccgaac ttgcacgtaa aggtgtaacc aaggaacagg ctatgcgtgt
     1561 gaaacagcta tacgagcagc agaacaatgt aaacacttcc caatccaccg gtacggatat
     1621 caacgagtcc cgccttcgtg aagagactaa ggaaaatact tccgacatgc tggaggacca
     1681 tcccaccact caggaccttg cccgtggcga tcaggtcttc ggacgtaaca tcttcaatac
     1741 ccgcaacctg acatttgaac ccagtgtcaa tctcgccact ccctccaatt accgtcttgg
     1801 tcccggtgat gaagtcatta ttgatatctg gggtgcgagc caaaacacga ttcgccagca
     1861 aatatctccg gaaggtacca tcaacattca gaagatcggc cctgtcaacc ttagcggtat
     1921 gacagtctct gccgccaatg actatctgaa gaacgccctg aataaaatat ataatggtct
     1981 gaataatact accgacccta cttccgacat ccgtctgact ttgggtaata tccgtactat
     2041 tcagatcaat gtgatgggag aagtcgtaca gcccggtact tatgcccttt cttctttctc
     2101 taccgtcttc catgcgcttt atcgtgcagg tggtgtgagt gatatcggta gcctccgaaa
     2161 cgttcaattg gtacgcaacg gaaagaatat tgccaccatc gacgtctacg aattcatcat
     2221 gaaaggaaat acactggatg acattcgcct tcaggaaggt gacgtcgtga ttgttcctgc
     2281 ttatgatgtc ttggtaaaga tcagcggcaa ggtaaaacgt cctatgcgtt ttgaaatgaa
     2341 gaaagacgag aaccttgcta ctctcatcaa atatgccggc ggctttgaag ctgacgccta
     2401 cacccgttca ttacgtgttg tccgtcagaa tggtgaggaa tacgaagtca acacagtcaa
     2461 ggatatggac tacagtatct acacaatgcg taacggtgac gttgtaacag ccgaagccat
     2521 ccttaaccgc ttcacaaaca aactggaaat ccgtggcgct gtctatcgtc ccggcatcta
     2581 ccagctcagc ggtaaactga acacgatccg cgaacttgtc catgaagccc agggcctgac
     2641 cggtgacgct ttcctgaatc gtgccgtcct ttatcgccag cgtgaggatt tgaccagtga
     2701 agttgttcag atagacatca ggtctatcat ggacggtact tccccgaacc tcgccctgat
     2761 gaagaatgac atcctttaca ttccgagcat tcacgatctg gaagatcgtg gcaatgttac
     2821 agtttacggt gaggtcgccc aaccggattc ctatccctat gctgataaca tgacacttga
     2881 ggatctgatc attcaggccg gtggcttgaa agaagccgcc tccactgtcc gcattgatgt
     2941 gtcgagacga atcaagaatc cccgtagtac cgcggataat gatactatcg gtcagatgta
     3001 taccttctct ttaaaggatg gttttgtcat tgacggtcag cccggtttta tccttcagcc
     3061 ttatgatgaa gtttatgtcc gtcgcagtcc gggttatcag gcacaacaga atgtagtcat
     3121 tgatggtgaa atccttttcg gaggaaatta tgccatgacc agccgtgagg aacgtctttc
     3181 cgatctggtg aataaagcgg gtggtcctac taaccttgcc tatcttcgtg gtgcaaagct
     3241 gacccgtgtg gcaagtgccg gtgagaagaa acgtatgggt gatgtgattc gcctgatgag
     3301 cagacaattg ggagaggcca tgattgactc tttgggtatt ggtgttgagg atacctttac
     3361 ggtaggcatt gatctggaga aggctttgac taatcctaag ggtagtgccg accttgtctt
     3421 acgtgaaggt gatgtgatct ccatacctaa gaatactaat actgtaacta tcaatggtgc
     3481 tgtcatggtt ccgaatacgg tttcttatat gaagggaaag aatgtggatt attacctgaa
     3541 tcaggccggt ggctgttctg ataatgcaag gaagagtaag aagttcattg tttacatgaa
     3601 tggtcaggtg acgaaggtta agggtagtgg taagaaacag attgagccgg ggtgtgagat
     3661 tattgtgcct ggtaaggcta agaagaaagg gaacatagct aatattttag gttatgctac
     3721 ttccttcagt tctttgggaa tgatgattgc ttctattgct aatttgataa agaaataaat
     3781 gacctattat gattgaaaag caatcttatc aagaaccgtt ttctgaagtg aataaaagga
     3841 aagatgatga aatggaaata gacttgatgg ctatttttta taagattatt actgttcgtg
     3901 gagtactatg caaagctgct ggagtgggag taataattgc tatagtggtg gcattgagca
     3961 ttcccaagca atatacagta aaggttaccc tttctcctga aatgggtaat gcgaagggaa
     4021 gtaatggttt agccggactt gctgcctcgt ttttgggaga tggggctcta ataggggaaa
     4081 gcacagatgc cttgaacgct tctctttcag cagatattgt ttcttcaact ccttttttat
     4141 tggaactttt agaaatggaa gtctccgttt ctaaaaagga tgataaaatg actttggggt
     4201 cttatctcga tgaagagtca tctccttggt gggattatct tataggtttt ccgggcatag
     4261 taataggagg agttaaatca ttattcggag aagatacggt tcctgccagt ggagggcggc
     4321 aaggaactat cgaattgacg aaagaagtaa atgagaagat taattttctt aaaaagaaga
     4381 ttagtgcatc cattgataag aaaaccgcca taacgagcat tacggtcacc ttacaaaatc
     4441 cgcagatcac tgcggttata gcagattctg tcgttcataa attacaggaa tatattattg
     4501 ggtatcgaac ttcaaaagta aaagaagact gtgcgtattt ggaacggttg tttaaagaac
     4561 gtcagcagga atattatgct gctcaaagaa aatatgcgga ttatgtagac gcccatgata
     4621 atgtggttct tcaaagtgta cgtgctgagc aggagcgttt acagaacgac atgagtttgg
     4681 cttatcagat atatagtcag gttgctaatc agttgcaggt atccagagcc aaagtacagg
     4741 aggaaaagcc agtctttgca gtagttgagc ctgccgttgt acctttgaaa ccttcaggta
     4801 tgggactcaa aatttatatt ttattatttg tgttcctgtc cgtatctggt actctcgtgt
     4861 gggtgttttt tggcaaaagt ctgctggcta gcttgagaaa agaactgaaa aataaggaga
     4921 tagaacagga taaatgatgc taaaacgtcg tttacaacat tcttacatca attaaattca
     4981 cttactatat tattaaacga atgatacgta cagctatgat aatggctgcg ggcatgggaa
     5041 cccgttttgg ccaatataca gaattgatac ctaaagggtt cgtcaaagtg ggaggtattc
     5101 caatgatagt tcgttctatt gatactttgt tatcatgtga cattgagcga attgtaatcg
     5161 gtacaggata taaacaagaa gtttatgaag aattgaagac agactatcct atgcttgaaa
     5221 cctgttttag tcctcgttat gctgaaacaa atagtatgta tactttatat aatacgcggg
     5281 agattctggg caatgatgat tttttattgt tagagtcaga tttgattttt gagaaacaag
     5341 caataatgag cttattggag tgtcctgcgg cagatgcaat gctaataact ccagtgacaa
     5401 agtttcaaga tcagtattat gtggaacatg atgataattt tcgcctttct tcatgttctg
     5461 tcaataagaa taaactaaac gctaaaggcg aattggtcgg catccataaa ctatccggtt
     5521 ccttttacaa aataatgtgt gctgattatg cgagtattgt tgatgaacaa cccaatttgg
     5581 gatatgagta tgaattacta cgaatatctt gttctcaatc tcctgtttat gtgcataaag
     5641 tagaagggct gaaatggtat gaaatcgatg acatatcaga tttggaatat gcagagaaat
     5701 atattgttcc gtattgttaa ttaaaataat atatcaaatt atgagtaaga cagtttatat
     5761 tgggatgact gcggatatta tgcatccagg acttatccgc attattaatg aggctactaa
     5821 atacggtgat gttataattg gtcttcttac agataaagct attgcggagc ataaacgcct
     5881 tccttatctg acctatgaac aacgaaaaga ggtagttcag aatattaaag gcgtatgtaa
     5941 agttgttcct caagaagaat ggagctatgt tgagaatctc aaacgcatta aacctgacta
     6001 tataattcat ggtgacgact ggaaaaccgg gcctttacgt gaagaacgtg tgcgcgtatt
     6061 tgaagtgatg aatgaacagg gaggaaaggt tatcgaaatt ccatatactt tgggaattaa
     6121 ttcatcttct ttggataaag atattaaggc tataggtaca acccctgatg tacgtttaaa
     6181 atcacttcgt cgtttgataa atgccaaacc cgtagttcgt attttggaag ctcatgatgg
     6241 actatgtggc ttgataattg aaaatcagga aatattaaaa ggggataaga gggaagtatt
     6301 tgacggaatg tggtctagta gccttacgga ttcaaccagt aaaggtaaac ctgatattga
     6361 ggcggttgat ttaactaccc gattgcaaga tttgaataat attctggagt gtactactaa
     6421 acctattatt tttgatggag atacaggtgg taaaattgaa catttcgtat ttacagtacg
     6481 cactttagaa cgtcatggaa tttcagctat aataattgaa gataaagttg gattaaaaaa
     6541 gaattcattg tttggtacag atgttattca aactcaagat acaatcgaag gtttctgtaa
     6601 taagatcaaa gcaggaaagg catctcagat tactgatgat tttatgatta tcgctcgtat
     6661 tgagagcctt atagcgggaa agccggtaag tgatgcttta gagagagctt ttgcatatgt
     6721 ccaagcggga gcggacggta ttatgataca tagcaaaaat aagtcgggtg aagatataaa
     6781 agaattttgt ttggcgtttc gaaaacagta tgctcatgta cctatcgtgg ttgtgcctac
     6841 tacatatgat catatttatg agtctgaatt gtgtgattgg ggtgtgaata taattattta
     6901 tgctaatcat atgcttcgtg ccgcttatcc tgctatgatg aatgtcgcaa agataatatt
     6961 agagaatgaa cgtgctttag aggtgcgcga actgtgtatg cccataaaag aaatattgga
     7021 actgattcca ggtactaaat aaaaagagga atatcattat gttaagcgta gaaaaagttt
     7081 atgaaacgtt tctgagtcat gacgttgatt tttttacagg agttccagac tcattactaa
     7141 aaaatatttg tgcctatatc acagaccata cttcacgtga gaaacatatc atagctgcta
     7201 atgaaggtgc ggctgtagga attgcttcag gttattatat ggcatcaggt aaggttcctg
     7261 tggtttatat gcaaaattcc ggcttgggta atactgttaa tcctctgctg tctcttgcag
     7321 atgaacaagt atacagtttt ccaatgcttt tgatgattgg ctggcgtggt gaaccgggaa
     7381 caaaagatga acctcagcat aaaaaacagg gtgaggtaac tttagatttg ttgaaagcaa
     7441 tgcggattcc ttatataatt cttgattcaa atgagaatga tgcatttatt cagctccatg
     7501 atatcataca atcagcaaaa actaagaata ttcctcatgc aataattatt cggaaagatg
     7561 tttttggcaa atataaactt caaaaagagt tcggatgtaa ttatccttta tctcgtgagg
     7621 atgctttgca aatagttgtg gattatctgc ctgaaaattc agtggtcgtt tctactactg
     7681 gcaagctttc tcgtgagtta tttgaatatc gagaaatgaa agagcaaggt catgaacatg
     7741 attttctcac ggtaggctct atgggacata gttcatcaat ctctttgggt attgctatag
     7801 caaagcaaga tagacctgtt tattgcttgg atggtgatgg agcatttatt atgcatttag
     7861 gggcaattag taacattggc gatttatctc ctaaaaacta ttatcatatt ttgtttaaca
     7921 atggtgctca cgagtctgta ggaggacagc ctactttagg atttagttta gatattccgg
     7981 ctattgtccg ggggagcgga tataagcata cttatactgt ttgtacaaaa gttgaaattg
     8041 aagaagcaat gaagcagctt ccaaagttat gtggtcctgt tttgttggaa ataaaggtta
     8101 aaatagattc tcgggagaat ttgggacgtc ccacgactac tcctatagag aataaggagc
     8161 attttatgga ttttttaaaa taaatatatt cgatgaaaat agaaagaaaa atattattaa
     8221 atcctgggcc tgccactact actgacagtg taaaaatggc acaagttgtt ccggatattt
     8281 gtcctcgcga gaaagaattc gcaggtatga tgaaacaact tcgggacgat ttggtcagag
     8341 ttgctcatgg gaatttggaa aaacataccg ctgtattatt ttgtggctca ggaaccatca
     8401 atattgatat atgtctaaat tctcttcttc ctgaagacaa aagggtgctt atagtgaata
     8461 atggggcata tagtacccgt gctgtagaag tgtgtcaata ttatggtttg cctcatatta
     8521 atttggaatt ttccgtttac gaacgtcctg atttatcggt ggtggaaaat gtgttgcaag
     8581 aaaatcctga tatagctatg gtttatacta ctcatcacga gacgggtaca ggaattctga
     8641 accctattcg cgagatgggc gctatagctc ataaatatca ggctaaattt gtcgtcgata
     8701 ctacttcttc tttgggcatg attccttttg atatagagaa agataatgtg gatttttgta
     8761 tggcatctgc tcaaaaagga cttcaagcga tgacaggtct ttcttttatt ataggcaatg
     8821 aggaacagat taagttttca aaaaaatatc ccaagcgttc ttactattgt aatctttatt
     8881 tgcagtatga gaattttgag agaacaggtg aaatgcattt tactccaccg gtacaaacta
     8941 tttacgccac tcgtcaggca ttaaatgaat attttgaagt gggtgaagaa gctaagtttg
     9001 cccgtcacaa aagagttttt gaggctattc attcaggtat aaatgaaatt ggtcttaaaa
     9061 gtgtaataaa gcgtgaatgg cagtcaggac tggttgtctc agttcaatac ccagaagatc
     9121 ccaactggga ttttgagaaa atacatgatt attgttatga gcgtggattt acgatttatc
     9181 caggtaagat atcaactgag gatactttta gaatctgtgc tttaggagag atagaagtag
     9241 atgatattga gaacttcttt aaagtactaa aagctgcatt aaatcattat aacgtcacat
     9301 taccgatgtc atgattgcag aaatattatt gaagattcgt catttttatt tttcccttcg
     9361 tggtatacac ttccatccgg aaagtgttat ttctggagtg cctattcgta gctttcgctt
     9421 agtaggcaaa tatggcaata ttaatttgag gtgatgcatt aggagttctt tattcttata
     9481 aaaaggcatc ggtagttata ggagataatg tttgtgtagg gtaatttgct tttagtggga
     9541 gagaccatat tagaattgcg gtaaggggcg aagaagataa taaccaatta gtgagaatat
     9601 tggaaaaata taattgatat cggtaaatgt agttgttttt atactgtatt taagtaatgg
     9661 aaaatctaaa gcagaaaact gtatcaggaa taatgtggag cgctatagaa cgtttctctt
     9721 tacaaggagt acagtttata attcaaataa tattagctcg tttgttacta ccatcggatt
     9781 atggtatgat cgggatgtta gctatttttc ttcaagtcgc tcaagtattt atagatagcg
     9841 gttttacaaa tgctttaatc cagaaaaaag atagaactga agaggatttt gccactgtct
     9901 tttattttaa tattcttgtc gcagtattat tctatggtat attgtttttt tcggcttctt
     9961 tgattgcaga cttttataat atgccgactt tagttcttgt aattcgtttt atagcattgt
    10021 cgttgataat aaatgcttta tcggctattc acagaacgaa attaattatt tctgtaaact
    10081 ttaaaactca atctaaaatt tcattaggtg cagcttttat atcaggcgtg attggaatat
    10141 ggatggctta tgtcggttgc ggggtttggg ctttagtttg gcaaactctc ttaaatagta
    10201 taattttgac aattttgttt tactgtttag ttcattggaa acctttacat actttctcca
    10261 aaagttcttt tgaaagatta ttcaatttcg gatctaaatt attgatctca agtcttatca
    10321 atacagtata tcgtaatctg tataccattg taataggaaa aaagttttct gttgtagagc
    10381 ttggttacta tacaagagca gaacaatttg ctatttttcc ttccaataat ttaaatgctt
    10441 tagtttctag agtagcgtat cctattttaa gttccattca agatgatgat aaatgtttaa
    10501 caaacgcata tcgaaattat atcagattat cctcatatat aatttttcct ttaatggtag
    10561 gtttacttgt tttggctaat cctattatta gtctattgtt aacagataaa tggtctgggg
    10621 ttgttattct tttacagata ttatgtttgg attggatgtt tgatcatttg agccaaatta
    10681 atttgaattt attatgggtt aaaggaagat ctgatctgtc gttacgtctt gaaataataa
    10741 aaaagacgat tgctatattc attctttttg tgtcaattcc atttgggctt gaggtgatgt
    10801 gttggggtag agttatttac tctctgatag cgacatattt gaatacatat tatactaatt
    10861 ctcttattga tttgacctta aagttgcaag ttaaagatgt atttccaagt ttaatactct
    10921 ctttcataat gggggggatc gtttttatat gtatttcttt ctttgaaact gatatagtaa
    10981 aaattgtgat tggatgtatt actgggatgt ccttctatat tttactttct tttctttttt
    11041 aagctagatt cttttttttg tattttaaca ttgatcatta aaactaaaaa catagatgaa
    11101 aatattaaaa aagatttttc aagtaatagt aaagataatt aaccattgtg tgccagttga
    11161 tagtagatca ttgtatttta ttcctcatcc taattgtaga acagataaat atgatattat
    11221 aaattattcg tcagataatg ttctcgttct gttaaattat ttgctaaaaa aagatagtgc
    11281 tgaatattat aaaatatatg ttgaaattta tgattcttct cgtatttcag aatatcaaga
    11341 atatatttat ggcataaatc ctaatattag gtgtactttt ttatgtgcat gtgttggacg
    11401 ttttttcttt aataaagtat cttttaagga tatgttgtat gccttttttt gtttttgtag
    11461 agcaagtaaa tgttttacag ctacttttta ttatgatttt tcatttaaaa aacgctctca
    11521 acagattatt tgtttggggt attatactcc attcaaagat gattatcata tgggagatca
    11581 ttcttatgat aaatttcgta atacaacatg taactcattt gattactcta ttgctacttc
    11641 ttgtttaagt gctagaataa tttcaataga ttgtggaatc tcatatgata aattcaaagt
    11701 cttaggattt cccagaaatg atttgttgat atcgaaaaat aattgcaatg ttattaaaag
    11761 agaaatttcg aaatttgcgg gatatgatgt aactaaatat atagtttata ctccaacttt
    11821 tcgtgattat gagactgtta ctgaaggtaa tttgcgttca atactcggat atgttgattg
    11881 tgatttgttg aagttatcga aaatactatt gaagtttaat gcggctttaa ttcttaaatt
    11941 acatcctttg cagaataaaa cagtcttaaa aaaagattta cccaaaggta tacttgtttt
    12001 tgaacaaaca tataaatatg gattgtatga tcttttgtca ttttcagatg gaatgattac
    12061 tgattatact tctacctatt ttgatttttt attggtaaat aagccagtca tatttaattt
    12121 ctacgatatt gaagaatatc gtagagtaag aggtttctca tttgagccaa tagaattttt
    12181 ttgtgcaggt gatattgttt acaattataa tgaattgata gatgctgtga tgggacttct
    12241 ggctgggaaa gatattcatg ctgaacatcg acgtcatatt tctttattga tgaatcaatt
    12301 tcaagatgat aattcaacaa agagaatatg tgacatattt ttaaatgaaa tataataaat
    12361 aaaatgaatt atgcatttgt ggtgcaggca cgtttaggtt ctacacgttt gcctggaaaa
    12421 atattgaaac ctttctatgg taatcaatcg attcttgact taatggttca taagctttct
    12481 gctatatcga atattccagt aattattgct acaacaaatt ctgttatcaa tgaacctatt
    12541 gaaaagaagg cacttgcatt aggggtaaag tgcttcagag gtgaggagaa tgatgtttta
    12601 aaacgattca ttgatgttgc tgaatatttt gatatacagg gtatattccg aatatgttct
    12661 gataatccat ttttagatgt acatgcggca aggcaattgg tcgaaatagc aatgaaatct
    12721 tgtaacgatt atataagttt tgatattgat ggtactccat caattaaaac tcatttcggt
    12781 ttttggggag aatttgtaac gcttgatgcg ctgaagcgag ttataggatt tactgatgat
    12841 ttattgtatc gtgaacatgt tactaattat atatatagtc atcctgaact ttttaatatt
    12901 caatggattt caggatctcc agtcgtgtca aaacatcata atattcgact cacgattgat
    12961 acgttagaag atttttctgt agcacagaga atatataggg atttacaaga aaaaagagtg
    13021 gaaatatcga tagaagtgat tgtagactat gttcgaagac atgcagaata cattcaactg
    13081 atgaaacaac aaataaaaaa aaattctaaa taatgaagaa tacttatatt attggtgaaa
    13141 tagggcagaa tcataatggt tctgttgact tggctaagtt gattattgaa ttaatctcac
    13201 gacctgtaca tgaggatgtt tttggtatgg atttcatgcc aatgaatgct gttaaaatga
    13261 ctaaaagaga tttatcagaa gaattggctg tttctcaaat gaatcaaata tatgacaatc
    13321 ctaattcttt tgggcgtact tatggtgaac atcgtgcttt tcttgagctt gatgatcaag
    13381 cgcattttga gatttataaa tatgctaagt cattaggatt agattttatc gagacgttat
    13441 gtgccaaagg atgtctgtct ttgcttaaac tgttcacacc ggataggcta aaagttgcta
    13501 gtcgtgattt gactaactta ccattgcttg aagcattagc agaaactcat attcctatta
    13561 tattatcgac aggaatggct ggtcaaagag aacttgacaa tgcattagat gtgataacga
    13621 gatatcatag taatatagct attctacatt gtgtatctca gtatccaact catccaaata
    13681 atttgaatct acgtacaata acatatttga agaagcatta caataagttt gagataggtt
    13741 tttctgatca tacaataggt atttctgctg ctacagctgc tgtagcgatg ggagcggaaa
    13801 ttatagaaaa gcatgtaaca attgatcggc atatgaaagg aacagaccag cttggctctt
    13861 taggtccaga tggggttaat cgtatgattc gagatattcg catagcagag tgttggcttg
    13921 gtactgaaga tctttatata gaaaaagggg tggagaagtc taagataaaa cttgaacgtt
    13981 ctattgccac tcgaaaatat attccagcag gtagtattat tcaagagtcg gacattcatt
    14041 tactgagtcc tggtgatggt tataaatgga tagataaaaa aaatgtaatt ggtcattttg
    14101 ctaaacaaga tatatcagcg aatgaaataa tatatcctga ttttattgaa tagatatatt
    14161 cctactgcaa cttgtattac ggtatagagt actgcctgct ataagctaag tagtgattaa
    14221 taaataaggg taaaatacta aaaaaatgaa agagataaag cttatactta cagatattga
    14281 tggcgtttgg actgatggcg gaatgtttta tgat
//
