LOCUS       gnlProkkaNLDOMKLD_4    12823 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NLDOMKLD_4.
ACCESSION   gnlProkkaNLDOMKLD_4
VERSION     gnlProkkaNLDOMKLD_4
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 09:10:03
            Original ID  :: gnl|Prokka|NLDOMKLD_4
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 3448829
            Orig. end    :: 3461652
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12823
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7823]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7823
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..12823
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12823
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="2"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             85..774
                     /locus_tag="ctg4_2860"
                     /transl_table=11
                     /translation="MKLGIMQPYFMPYIGYFQLIKAVDKYVVYDDVNYIKGGWVNRNNI
                     LVNGEKKMFTITLKGASAYKHFNEIEIGDDFRKFMKTLELNYSKAPFYHETMELLYRII
                     MFPNRQLALFLANSFKEILDYLNIQTEILLSSDLPKDCSLKGKDKVLHICKLLSADTYY
                     NAIGGKQIYNKEDFMQNGINLKFVDTIIKRYPQQSKEFVPYLSIIDVLMNNSKEETCML
                     LDSFSLQ"
     CDS             774..1736
                     /locus_tag="ctg4_2861"
                     /transl_table=11
                     /translation="MSIGGYFELEIREGKHFHQGAIKLSTARQCFEYILKARRYTKVYI
                     PYYTCDVIIEPLMKLNVKYEYYHINSLLDPVFDKKIESSEAFLYTNYFGLKQDTVLYLS
                     RFYPNLIVDNAQAFFAKPIEGVDTFYSARKFFGVPDGAYLYTTALLDIDFPIYNPIDNI
                     NYLIGRITDSAEGYYKDFQESEERMSNQEIHRMSVLSDKILSSLDYVSIIERRRFNYHL
                     LYSALTAFNKFDFSISENQNVPLIYPFYTSKAGLRQQLILEHVYVPQYWQNVVNKIDAK
                     CLEYSIANNMIALPIDQRYDEKTMNILIEKISNLNNHLS"
     CDS             1733..1921
                     /locus_tag="ctg4_2862"
                     /transl_table=11
                     /translation="MKKKIIIFGATGNTGSYLLEYALNFFNKEEYEIIASGRRETTFFF
                     QKGNTILFCGHYEGRRF"
     CDS             1953..2729
                     /NRPS_PKS="Domain: NAD_binding_4 (23-160). E-value:
                     3.4e-07. Score: 21.4. Matches aSDomain:
                     nrpspksdomains_ctg4_2863_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 88;
                     E-value: 1.3e-26)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg4_2863"
                     /transl_table=11
                     /translation="MLLSAQIPSYMDTYCPIKYLDSIVIGGFNVLEYCRKCHVDRIIYT
                     QTVFDISLYSHDTILKPDMKPNFSYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRL
                     PTIYSYSPYPYYFPNGVKTKRPIYQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQMLC
                     KAVEVEQESGFYNVGTGMPITLEEQIRTIISVFSPKESPSEVIYRPDKVCGGGYIMNIE
                     NAKKELGYVPQYDCRKLFEDYKSEMEIKRFAELRLK"
     aSDomain        2022..2432
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg4_2863_NAD_binding_4.1"
                     /evalue="3.40E-07"
                     /label="ctg4_2863_NAD_binding_4.1"
                     /locus_tag="ctg4_2863"
                     /protein_end="160"
                     /protein_start="23"
                     /score="21.4"
                     /tool="antismash"
                     /translation="VIGGFNVLEYCRKCHVDRIIYTQTVFDISLYSHDTILKPDMKPNF
                     SYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVKTKRPI
                     YQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQ"
     CDS             2730..3662
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg4_2864"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.4e-23,
                     bitscore: 80.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGSITVSICCLTYNHEKYIRQCLDGLIMQKTEFDFEILVHDDAS
                     SDATQSIINEYVVAYPNLIKPILQIRNQYSQGISPLRQILFPLVKGKYVALCEGDDYWT
                     DPLKLQKQVNAMESDHSLAGCAHQSLVFYENGTPSHMFHHVKKQILKTKDLLDVRLFHT
                     ASFLFKKEVLDYIKDFNSSLYAGDRFLFLACSLKGNILYMPDVMCCYRKNAMGVTSRVS
                     YSLHVLDLKVPEYFHNKDLKFPYYRYLAFVHRAIFSESLDISLKKYIYHFTLFVKYSFS
                     YFPKNLKDIYKALYVSFWWLTKGQKYRIK"
     CDS             3659..5038
                     /locus_tag="ctg4_2865"
                     /transl_table=11
                     /translation="MIAIIFLILFLIQILISNRLIIDSFSRKISFIYLLWWGTAIVIST
                     FDPAELYSVSMKTYMLLLINVWAFNWGFLCFGKIKKIRLKVTTDNSFYNLVIGNNKMIL
                     LLMLACIVTYYYAMKFALVIELYGAGEGRMMRFEAGELFTSNLEVYLYDFLIKPFTQFV
                     MLIVACMLLSKIKRRILLLSVLFLFLYASVGNGRGGLIVFLFYCFIIYYLRRELYYINL
                     LASKRKKWNFGVVSFVCLFVGVGIIVVVISYMTAQRMGFDTFSAEVVKIGFEELKSQAV
                     WYFLGPFRALDYSFRANYESITGFWYGRATMGGIDNLLKLVIHYLGGSYTIAHDEIGHL
                     LQNSKISVGNGIIFNYAYTNIIFHYLDFGYVGVLLFPFLYGFIVRYSIYKFTQKPTLLT
                     LGIMIYLVYISMFNIFSWEFQKPSSIIYVLLLLFFQYVYSHKFVILKLNGKKKGLCRHE
                     C"
     CDS             5001..6305
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 202.6;
                     E-value: 1.3e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_2866"
                     /sec_met_domain="CTP_transf_like (E-value: 5.6e-10,
                     bitscore: 37.5, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.8e-117, bitscore:
                     388.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 9.3e-62, bitscore:
                     206.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKKVYVGMSADIIHPGHLNIIHEASKLGHVTVGVLTDSAIASY
                     KRLPYLNFDQRSLIVLNLKGVDEVIPQTTLDYVPNLELIRPNFVVHGDDWKEGIQQETR
                     QRVIDTISKWGGKVIDVPYTKGISSTLLNTRLKEIGTTPEIRLKRLKRLIAAKSIVRIC
                     ESHSGLTGLIIENTSVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDA
                     LECTTKPVIFDGDTGGKIEHFVFTVRTLERLGISAVIIEDKIGLKKNSLFGTDAVQTQD
                     TIEGFCDKIQAGKRAQITDDFMIIARIESFIAGKGLDDALGRALAYIKAGADGIMIHSK
                     DKSGEDIKNFCLALRKIHKSIPIVVVPTTYNHISEDELASWGVNVVIYANHMLRSAYPA
                     MLNTAKSILANARSYEANDLCMPIKEILELIPGTK"
     CDS             6337..7251
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 45.3;
                     E-value: 8.9e-14)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_2867"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.6e-15, bitscore:
                     53.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.4e-12, bitscore:
                     45.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPNIFVDSLGAMQMDFFTGVPDSLLKNICAYLTDHLASEHNII
                     AANEGAAIGLATGYYLATRKIPVVYMQNSGLGNAVNPLMSLTDKEVYNIPLLLLIGWRG
                     EPGVKDEPQHIKQGKVTLPLLEAMGIRYAVLSQDEKKFISQLNVAKSYMDETREPFAFV
                     IQKDTFLEYSSENCEDERLSLKREAAIHFIAASLTEKDIIISTTGMISRELFEYRESTS
                     LEHERDFLTVGSMGHASQIALGIALKKRDCKVYCFDGDGSTLMHMGSLAIVGSLHPENY
                     VHVVFNNGAHDSVGGTTNSRFRY"
     CDS             7476..7823
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_2868"
                     /sec_met_domain="Fe-ADH (E-value: 5.5e-19, bitscore: 66.1,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRFFLGESSIKELIPILQEYTPDHIFLVRGKNSYEKCGAQAVL
                     ESVFVKLACGVTEFFDFEANPKIEDMEKGLSLLKEYPTNMIIAIGGGSVLDMAKLLRFF
                     YSYSGNPTGGV"
     CDS             8305..8568
                     /locus_tag="ctg4_2869"
                     /transl_table=11
                     /translation="MRYNIGNSDILLNPNINPYLYKIKMQKMLQWLNMECVDVAIAMKE
                     YIRNLGLDIDISQIDISKIVSSVNIQRLINNPYKVEAICLEE"
     CDS             8553..9269
                     /locus_tag="ctg4_2870"
                     /transl_table=11
                     /translation="MSRGISEKEQRWLLVLSQVIEVLDKNNLLYFIDTGTLLGAVRDKM
                     FIPWDNDIDLAVIGGDTSTTNLLKVSKLLYALGFYVTLTKKEIAICSRDGVNLGIKLYK
                     KQASHYEACMGKVYRHRLFYSINLYLSGEVLYKKGFSFKYKAISLFVKFSQVFKLKLSC
                     IWQMKLLFWAKYDEKTVIIPFEFVDAIIDYNFYNIIVKVPNKPERYLAYRYGENWKVPK
                     QDYDYMIDDKSIINID"
     CDS             9424..10539
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg4_2871"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2e-10, bitscore:
                     38.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNILINCSNLKKGGGLQVADSVCSCLNTIPQHDFFLVLSSCMDDT
                     ADRVSEYKNVTVVRHDIRNSWYTLFWGRDKAMDNLVTRHNIGGVLTIFGPARWKPKCKH
                     LCGFARSHLVLNDSPYFQRMSWKELMIEKARNLYLYYYFKRGVTAFWTENPYISEKVKH
                     LFPTIKAYTVSNYYNQVFDDENTQIEHALPSFDGCTLLSINAPYPHKNLAIAIEVARYL
                     KKHYSEFSFRFVFTVTENDLPPIPSYLREHFCLIDKVSVAECPSLYRQADIMFQPTLLE
                     CFTATYVEAMRMQVPILTTDLPFAHGLCGNAAVYYSPLDAEDCAKSIYKMAVDTSFQDY
                     LVGEGIKQLKSFDNYNRRAEKLIDLISNLDD"
     CDS             10554..11627
                     /NRPS_PKS="Domain: NAD_binding_4 (10-144). E-value:
                     2.6e-07. Score: 21.9. Matches aSDomain:
                     nrpspksdomains_ctg4_2872_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 105;
                     E-value: 8.3e-32)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg4_2872"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.5e-106,
                     bitscore: 353.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-06, bitscore:
                     24.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFKDKVLLITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQD
                     DMRHHLQNPKVKFYIGNVRDRQSVDGAMMGVDYVFSAAALKQVPSCEFFPLQAVQTNVI
                     GTDNVLESAIAHGVKNVVVLSTDKAAYPINAMGISKAMMEKVAIAKGRALGEEAGTTIC
                     CTRYGNVMASRGSVIPLWVEQIKNGRPITVTDPNMTRFMMTLDDAVDLVIYAFEHGHNG
                     DLFVQKAPAATLDVLAQALKETYAQVDPKYGETEVKTIGTRHGEKLYETLVTREEMAKA
                     VDMGDYYRIPCDTRDLNYDKFFTEGNEDIARIEDYHSHNTARLDVEGMKKLLLKLRFIR
                     EDLGMEEKAKSAEIKSE"
     CDS_motif       10581..10637
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_2872_0001"
                     /evalue="1.70E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg4_2872"
                     /protein_end="28"
                     /protein_start="9"
                     /score="11.1"
                     /tool="antismash"
                     /translation="LITGGTGSFGNAVLRRFLD"
     aSDomain        10584..10985
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg4_2872_NAD_binding_4.1"
                     /evalue="2.60E-07"
                     /label="ctg4_2872_NAD_binding_4.1"
                     /locus_tag="ctg4_2872"
                     /protein_end="144"
                     /protein_start="10"
                     /score="21.9"
                     /tool="antismash"
                     /translation="ITGGTGSFGNAVLRRFLDSDIKEIRIFSRDEKKQDDMRHHLQNPK
                     VKFYIGNVRDRQSVDGAMMGVDYVFSAAALKQVPSCEFFPLQAVQTNVIGTDNVLESAI
                     AHGVKNVVVLSTDKAAYPINAMGISKAMME"
     CDS             11728..11949
                     /locus_tag="ctg4_2873"
                     /transl_table=11
                     /translation="MAATVLNSVQQHLLKMFAFDGSEERLNEVKEVLTKYFSQKLDDRL
                     NDLWDSGVLNQEKLDELRKIDVRTLPKR"
     CDS             11946..12350
                     /locus_tag="ctg4_2874"
                     /transl_table=11
                     /translation="MNRIVLDTNSLIQCISPKSRYRKIWDCFLDGTNQLCVSDEILNEY
                     EEILERLAGVEIAKLVIDVILNNPYTLFFTPYYNFNLIKSDPDDNKFVDCAIVANAKFI
                     VTEDHHFHILKQYNFPKVSFIGLDDFCHSL"
ORIGIN
        1 aatggcaaaa agtgtaatct gcttgccaat gtattgtgga ttgtcgaatg aagatgttga
       61 aagaatttta aatctgatat tgaaatgaaa ttaggtataa tgcaacctta ttttatgcct
      121 tatataggtt atttccagct aattaaggct gttgataaat acgttgtata cgatgatgtt
      181 aattatatta aaggagggtg ggtgaatcgt aacaatatat tggtaaatgg tgaaaagaaa
      241 atgtttacta tcactttgaa gggggctagt gcctataaac atttcaatga aatagagata
      301 ggagatgatt ttcgtaaatt tatgaaaaca ttggaattga attactctaa agctcctttc
      361 tatcatgaaa caatggaatt gttgtatagg ataattatgt ttccaaatag acaattggct
      421 ctcttcttgg ctaatagttt taaagaaatt ttagattatc taaatattca gactgaaata
      481 ttgctatctt cagatttacc taaagattgc tctttaaaag gaaaggataa agttcttcat
      541 atttgtaagt tactatcggc agatacttat tataatgcta taggtggaaa acaaatctat
      601 aataaggaag actttatgca aaatggtatt aatttgaaat ttgtagatac gataataaaa
      661 aggtatcccc agcaatctaa agaatttgtt ccatatttat caataataga tgtactaatg
      721 aataattcta aagaagaaac ttgtatgtta ttagattcat ttagtttaca atgatgtcaa
      781 taggtggcta ttttgaactg gaaatacgtg aaggaaaaca ttttcatcaa ggtgcaataa
      841 aactgagtac tgctcgtcaa tgctttgagt atatattaaa agctagacgg tatacaaaag
      901 tgtatattcc atattacact tgtgatgtga taatagaacc tctcatgaaa ttaaatgtaa
      961 agtatgaata ttatcatata aatagtttac tggatcctgt gtttgataaa aaaatagaaa
     1021 gctcagaggc ttttctatat actaattatt tcggcttaaa acaagatact gttctttatt
     1081 tatctcggtt ttatcctaac ttaatagttg ataatgcaca agcttttttt gcaaaaccaa
     1141 ttgaaggggt agatacattt tattctgccc gtaaattttt tggagttcca gatggcgctt
     1201 atttgtacac aactgcgtta ctggatattg atttcccgat ttataatcct attgataata
     1261 ttaactactt gatagggcgt ataactgact ctgcagaggg gtattataaa gattttcaag
     1321 aatctgaaga aaggatgtca aatcaagaga tacatagaat gtctgtattg tctgataaaa
     1381 ttttgtcttc tttggattac gtctcaataa ttgaaaggcg tcgttttaat tatcatcttc
     1441 tctacagtgc attaactgca tttaataaat ttgattttag tatttctgaa aatcaaaatg
     1501 tcccgttgat atatcctttt tatacgagca aagctgggct tcgccaacaa ttaatattag
     1561 agcacgtata tgtacctcaa tattggcaaa acgtagtgaa taagatagac gctaaatgtc
     1621 tggaatactc aatcgcgaat aatatgattg cattgcctat agatcagaga tatgatgaaa
     1681 aaacaatgaa tatattaatt gaaaaaatat ctaacttaaa taaccattta tcatgaaaaa
     1741 gaaaattatt atttttggag caaccggtaa tacagggtct tatttgttag aatacgcatt
     1801 aaatttcttt aataaggaag agtatgaaat aatagcttca ggacgtcgcg aaactacttt
     1861 ttttttccaa aaggggaata ccatattatt ctgtggacat tacgaaggaa gaagattttg
     1921 ataagcttcc aatggaaaat atacatgcag ttatgctact ctctgctcaa ataccttctt
     1981 atatggatac ttattgcccc ataaagtatt tagattctat tgtaatagga ggatttaatg
     2041 tattagagta ttgccgcaaa tgtcatgtag ataggataat ttatactcaa accgtttttg
     2101 atatttcatt gtattctcat gatactattt taaaacccga tatgaaacca aattttagct
     2161 atacgggtga tcatgctata tatgtaattt ccaaaaatac tgctttagac ttgatagagc
     2221 attattatca agaatacggt ttgaaaaaat tcatttttag gcttccaaca atatatagct
     2281 atagccctta tccgtactac tttcctaatg gagtcaagac gaagcgacct atttaccaaa
     2341 tgattgaaaa tgctcagaaa ggactaccta tagagatatg gggagatcct cattatgcaa
     2401 aggatatggt tcatgttaac gattttagtc aaatgttgtg taaagctgtt gaggtggaac
     2461 aagaaagtgg cttttataat gtgggtacag gaatgccaat tacattagaa gaacaaattc
     2521 gcactattat tagtgttttt tcgcctaagg agtcaccatc tgaagttata tatagaccag
     2581 acaaagtttg tggtggtggg tacataatga acattgaaaa tgcaaagaaa gaattaggat
     2641 atgtgcctca atatgattgt cgtaagttgt ttgaggatta taaaagtgaa atggaaataa
     2701 agcgttttgc tgaattaaga ttaaaataaa tgaacggttc aataacagtt agcatatgtt
     2761 gcttgacgta taatcatgaa aaatatataa ggcagtgttt agatggcctt ataatgcaaa
     2821 aaacagaatt tgattttgaa attcttgttc atgatgatgc gtcttctgat gctactcaga
     2881 gtattattaa tgaatatgtt gtggcatatc ctaatcttat taaaccaatt cttcagattc
     2941 gaaaccagta ttctcaaggt atttctccgt tacgtcaaat attatttcct ttagtaaaag
     3001 gtaaatatgt tgcgctttgt gagggggatg attattggac tgatccgttg aagttacaaa
     3061 aacaagtaaa tgcaatggag agtgatcatt cgttagcagg ttgtgcgcat caatctttag
     3121 ttttttatga gaatggaact ccatcccata tgtttcatca tgtgaaaaaa cagattttaa
     3181 aaactaaaga cttattggat gtaagacttt ttcatacagc gtctttcctt tttaaaaaag
     3241 aagtacttga ttatattaaa gatttcaata gtagtttata tgctggagat cgttttctgt
     3301 ttttggcttg ttcgttgaaa ggaaatattt tatatatgcc agatgtaatg tgttgttata
     3361 ggaagaatgc aatgggagtt acttctagag tttcttattc attacatgta ttagacctaa
     3421 aagttcctga atattttcat aataaagatt tgaaatttcc ttattacaga tatttggcat
     3481 ttgttcatag agctatattt tcagaatcac ttgacatttc cttaaagaaa tatatatatc
     3541 attttacttt atttgtgaaa tattcttttt cttattttcc taagaatctg aaagatatct
     3601 ataaggcttt atatgtttct ttctggtggt tgacaaaggg acaaaagtat agaatcaaat
     3661 gattgctata atttttctta ttctattctt aattcagatt ttgatatcta ataggcttat
     3721 aattgattct ttttcaagaa aaataagttt catatattta ttgtggtggg gaactgctat
     3781 tgttatttct acttttgatc cagctgaact atattctgtg tcaatgaaaa catatatgtt
     3841 attattgatc aatgtttggg cgtttaattg gggatttctt tgttttggaa aaataaaaaa
     3901 aatacgattg aaggttacaa cagataatag tttttataat ctggttattg gcaataataa
     3961 gatgatatta cttcttatgt tggcatgtat tgttacttat tattacgcaa tgaagtttgc
     4021 tctagttatt gaactatatg gagcgggcga aggtcggatg atgagatttg aggctggtga
     4081 actttttacg agtaatttag aagtatatct atatgatttt ctaataaaac cttttacaca
     4141 gtttgttatg ctaattgtag cgtgtatgct actttctaag attaaacgta gaattctgtt
     4201 gctatcggtg ctttttctgt ttttatacgc atctgttggt aatgggcgtg ggggattgat
     4261 tgtttttcta ttttattgtt ttatcattta ttatttaaga agagaattgt actatatcaa
     4321 tttgttagcc agcaaaagga aaaaatggaa ttttggtgta gtttcttttg tatgcttgtt
     4381 cgttggcgtt ggaataatag ttgtggttat ttcatatatg acagctcaaa gaatgggatt
     4441 tgatacattt agtgctgagg tcgtaaaaat aggttttgaa gaactaaaaa gtcaggccgt
     4501 atggtatttt ttaggtccat ttagagcttt agactattca tttagagcta attatgaatc
     4561 gataaccggg ttttggtatg gaagggcaac aatgggtgga attgataatt tattaaaact
     4621 tgtaatccat tatttaggag gaagttatac cattgctcat gatgaaatag gtcatctttt
     4681 gcaaaattca aaaatttcag ttgggaatgg aatcatcttt aattatgcat acactaatat
     4741 aatatttcat tatttggatt ttggttacgt tggcgttctt ttattcccat ttttatatgg
     4801 ctttatcgta cgatatagta tttataaatt tactcaaaaa ccaacattac tgactcttgg
     4861 tattatgata tatttggtct atatatcaat gttcaatatt ttttcgtggg aatttcagaa
     4921 gccttcctca attatttatg ttttgttgtt gctatttttt caatatgttt attctcataa
     4981 attcgtaata ttaaaattaa atggaaaaaa aaaaggttta tgtcggcatg agtgctgata
     5041 ttattcatcc aggtcatttg aatatcattc atgaggcttc aaaattgggg catgtaacgg
     5101 ttggtgtatt aacagattca gctattgcca gttacaagcg tttgccttac ttgaattttg
     5161 atcagcgctc attgatagtt ctgaatttga agggtgttga tgaagtaata cctcaaacta
     5221 ctcttgatta tgttccgaat ttggaattga ttaggcctaa ctttgtagta catggtgatg
     5281 actggaaaga gggtattcaa caagaaactc gccagcgtgt gattgatact atctctaaat
     5341 ggggtggaaa agtgatagat gttccttata cgaaaggtat ttcttccact ttattgaata
     5401 ctaggttgaa ggaaatagga accactcccg aaattcgttt gaagcgactt aaacgcttaa
     5461 ttgctgctaa aagtattgtt cgtatctgtg aatctcatag tgggttaaca ggtttgataa
     5521 tagaaaatac cagtgtagaa gtcaacggag ttaagcgtga atttgatggg atgtggtcca
     5581 gtagtcttac tgattcgact tctaaaggta agcctgatat tgaagccgtt gacttgacaa
     5641 ctcgtcttca tgatttgaat gatgctttgg agtgtactac caaaccagta atttttgatg
     5701 gtgatacagg tggtaaaata gaacattttg tatttactgt tcgcacgctg gaacgtttag
     5761 ggatatcagc tgttattatc gaggataaaa taggtctgaa gaagaactcg ctttttggaa
     5821 cagacgctgt tcagacccag gatacaattg aagggttttg tgataaaatt caagcgggta
     5881 aacgtgctca gataacggat gattttatga taatagctcg tattgaaagt tttattgctg
     5941 gaaaaggttt agatgatgct ttaggacgtg cattagcata tattaaagca ggtgcggatg
     6001 ggattatgat ccatagcaaa gataagtctg gtgaggatat aaagaacttc tgtcttgctt
     6061 tacgcaaaat acataaatcc attccgatag tggttgttcc aactacttat aaccatatat
     6121 cagaagatga attggcttct tggggagtaa atgttgtaat atatgcaaat catatgctta
     6181 gaagtgcata tcccgctatg cttaatacgg cgaagtccat tcttgccaat gcacgctctt
     6241 atgaagccaa tgacttatgc atgcctatta aagaaattct ggaattgatt ccagggacta
     6301 agtaaataga ctctttaaaa ttaaattctt attattatga tttctcctaa tatttttgta
     6361 gactctcttg gcgctatgca gatggatttt tttactggag ttcccgattc tttattgaag
     6421 aatatatgtg catatttaac agatcacctt gcttctgaac ataacattat tgccgctaat
     6481 gaaggtgctg ctataggttt ggctacagga tattatttgg ctacgaggaa aattccagtt
     6541 gtttatatgc agaattcagg tcttggaaat gcagtaaatc ctttaatgtc attgacggat
     6601 aaagaagttt ataatattcc attgttattg ctaattggtt ggcgtggtga acctggtgtg
     6661 aaagatgagc cacaacatat taagcaggga aaagtcacac ttccattact tgaggcaatg
     6721 ggtatcagat atgctgtttt atctcaagat gaaaagaagt ttatatctca acttaatgtt
     6781 gcaaaatctt acatggatga gacaagagag ccttttgctt ttgtaattca gaaagacact
     6841 ttcttagagt atagttctga aaattgtgaa gatgaacggt tgtctttgaa acgcgaagct
     6901 gctattcatt tcatagcagc ttcattaaca gaaaaagata ttataatatc gacaacaggt
     6961 atgatatcac gtgaattatt tgaatatcgt gaatctactt ctttagaaca tgagagagat
     7021 tttttgactg taggttctat ggggcacgcg tcgcagatag cattgggaat agctcttaaa
     7081 aagagagact gtaaagttta ttgctttgat ggagatggtt ccactttgat gcatatgggt
     7141 tcgttagcaa ttgtcggttc tttacatccg gaaaactatg tacacgtagt ctttaataat
     7201 ggcgctcacg attctgtggg ggggacaacc aacagtcgct ttagatattg atttatgtaa
     7261 aattgctcaa gcttgtggtt atgaaatagt attgtctgtt tctgataacg aaggtttatg
     7321 tgaagctctt aaaaagatac agcaaatacg cggccctatt ttgttggaag taaaggttct
     7381 gaaagggagc tcgtaagaat ttgggaagac cagccaacac tcctatacaa aataaagagg
     7441 cattcatgct tttcttagat aatttataga gaattatgga acagcgtttt tttttaggag
     7501 agtcttctat aaaagaattg attcctatct tgcaagaata tactcctgat catatatttc
     7561 ttgtaagagg aaaaaattct tatgagaaat gtggtgcaca ggctgtgtta gaatctgttt
     7621 ttgttaaatt agcttgtggc gtgacggaat tctttgattt tgaagcaaat cctaaaattg
     7681 aagatatgga aaaaggactt tcattattga aagaatatcc gacaaatatg attattgcta
     7741 taggtggagg tagtgtatta gatatggcaa aattgcttcg ttttttttat tcctattctg
     7801 gtaatcctac ggggggagtt tgaaaagaag cgacaattaa ttccattaat aacacttcct
     7861 actacagctg gtacaggtag tgaagcaact catttttctg ttttatataa agataaagta
     7921 aaatattcag tagaacatga tgctattctt ccggatgtag cgattgtata tccaaaattt
     7981 acttatggaa attcaaaata tctaacatct tgtacaggct ttgatgctct agcgcaggcg
     8041 atagaagctt attggaatgt gaatgctacg gaagagtcgg ataaatatgc ggcgaaagca
     8101 attaaactct tatggcctaa tttgccattg gttgtaaatt ctcctacagc ggaggctagg
     8161 aataaggttt cggaaggctc ttattgggct ggtcgtgcaa ttaatataac taagactaca
     8221 gctccacatg ctttttcgta ttcatttaca actgattatg gatatccgca tggacatgca
     8281 gtagcattaa catttccctt ttttatgaga tataatattg gaaattcgga tatcttattg
     8341 aatcctaata taaatcctta cttatataag attaaaatgc aaaaaatgtt acagtggtta
     8401 aatatggaat gcgtagatgt tgcaatcgct atgaaagagt atataagaaa cttgggatta
     8461 gatattgaca tatctcaaat tgacatatcg aaaatcgtat cttctgttaa tattcaaaga
     8521 ttaataaaca atccatataa agttgaagcg atatgtctag aggaataagt gaaaaggagc
     8581 aacgatggtt gttagtatta tcacaagtta ttgaagttct tgataaaaat aatttattat
     8641 attttattga tactggtact cttttaggag ctgttagaga taaaatgttt attccttggg
     8701 ataatgatat tgatttagca gttattggag gagatacatc tactactaat ttgcttaaag
     8761 tgtcaaaatt gttatatgct ttaggctttt acgtcacttt gacaaagaaa gaaattgcta
     8821 tctgttctag agacggggta aatttaggaa ttaaattata taaaaagcaa gcaagtcatt
     8881 atgaagcatg tatgggaaaa gtttatagac atagactatt ttattctata aatttatacc
     8941 tctctggaga ggtcctgtat aaaaaaggct tttcttttaa gtataaagct atctcactct
     9001 ttgtgaagtt tagtcaagtc tttaagttaa aattgtcttg tatttggcaa atgaagttat
     9061 tgttttgggc taagtatgat gaaaagacgg tgattattcc ttttgagttt gtagatgcaa
     9121 ttattgatta taatttttat aatataattg tgaaagtgcc aaataagcca gaaagatatc
     9181 tagcatatag atatggtgaa aattggaagg ttcccaaaca agactatgat tatatgattg
     9241 atgacaagtc tattataaat attgattaaa aaaatatgaa aacaaatttc aacataagga
     9301 gtattattgt aattaataaa attataatca actttttctt tagacaatat agatggtatt
     9361 tataaatagc actttcattt atacaccaaa attgggcact tatatattat attctcggaa
     9421 caaatgaaca ttctaataaa ttgctccaat ctcaagaaag gtggagggct tcaagtcgct
     9481 gattcggttt gttcttgttt gaatacaatt ccccaacatg attttttttt agtattatcc
     9541 tcatgtatgg atgatactgc tgatagggta tcggaataca aaaatgtcac tgtagtgcgt
     9601 catgatatac gaaacagttg gtatacattg ttttggggac gtgataaggc tatggataat
     9661 ttggtaacca gacacaatat tggtggagtt cttaccatat tcggacctgc aagatggaag
     9721 ccgaaatgta agcatctatg tggatttgcc agatcccatc tcgttttgaa cgattctccc
     9781 tattttcaac gtatgtcttg gaaggaacta atgatagaaa aggcgagaaa tctttattta
     9841 tattattact tcaaacgtgg tgtgactgct ttttggactg agaatcctta tatttcagag
     9901 aaagtaaagc acttgttccc taccataaaa gcctatactg tatcgaatta ttataatcag
     9961 gttttcgatg atgaaaatac tcaaatagaa catgccttac cgtcttttga cggttgtact
    10021 ttactgtcca taaatgctcc ttatcctcat aagaatttag caattgctat cgaagtcgct
    10081 cgatatctta aaaaacatta ttccgaattt agttttcgat tcgtttttac ggtgacagag
    10141 aatgacttgc cacctattcc ttcttatttg cgagagcatt tctgtctgat tgataaagtt
    10201 agtgtagcag aatgcccttc cttatatcgt caggcagata taatgtttca accaacttta
    10261 cttgaatgtt tcactgccac atatgtcgaa gcgatgcgaa tgcaagtgcc aattttaact
    10321 acagatttgc catttgctca tggattatgt ggaaatgcag ctgtgtacta ttcgccgtta
    10381 gatgcagaag actgtgccaa atcaatttat aaaatggctg tagacacatc ctttcaagat
    10441 tatctggtcg gagaaggaat aaaacaactt aagtccttcg ataattataa tcggagagca
    10501 gaaaaattaa tcgatttgat atcgaatttg gatgattaat acatattata tttatgtcag
    10561 tatttaaaga caaagtcctc cttataaccg gaggtaccgg cagttttggt aacgctgtat
    10621 tgcgtcgttt ccttgattct gatatcaagg agattcgtat ttttagtcgt gacgagaaaa
    10681 agcaggatga catgcgtcat cacctgcaaa atcccaaagt aaaattttac attggtaatg
    10741 tccgtgaccg ccagtctgta gacggtgcca tgatgggagt ggattatgtg ttttccgccg
    10801 ccgctttgaa gcaggttccc agctgcgagt tctttccgtt gcaggctgtg cagaccaatg
    10861 tgatcggtac ggacaatgta ctggaatccg ccatagcgca tggggtgaaa aacgtagtag
    10921 tgctttctac cgacaaggcg gcttacccca tcaatgccat gggtatcagc aaagccatga
    10981 tggagaaggt tgccatagcc aagggccgtg cgttgggtga ggaagccggt acaaccatct
    11041 gctgtacccg ttacggcaat gtgatggcaa gccgcggttc tgtaattccc ttgtgggtgg
    11101 agcagataaa gaacggcagg cccattacgg taacggatcc taacatgacc cgcttcatga
    11161 tgacgctgga cgatgccgtg gatctggtaa tctatgcgtt tgagcacgga cataacggtg
    11221 acctgttcgt gcagaaggct cctgccgcca ccttggatgt actggcgcag gccttgaagg
    11281 aaacatacgc gcaggttgat ccgaaatacg gtgagacgga ggtgaagacc atcggcacgc
    11341 gccacggcga gaaactgtat gagacactgg taacccgcga agagatggca aaggctgtcg
    11401 acatgggcga ctactaccgc atcccctgcg acacccgtga tctgaactat gacaagttct
    11461 tcacggaagg caatgaggat attgcccgta tagaggacta tcacagccat aacactgccc
    11521 gcctggatgt ggagggcatg aaaaagcttc ttttgaaact ccgtttcatc cgcgaagacc
    11581 tcggaatgga ggaaaaggcg aagagcgcgg aaatcaagag tgaataagtc tttaatttga
    11641 ataatacaac aggaaagaaa gagggaatgt attgttcctt tagaaaaaaa aactatcttt
    11701 gccatattga aactaataat tgagaatatg gcagcaacag tattaaattc agttcaacaa
    11761 catcttttga agatgtttgc ttttgatggt tcggaggagc gattgaatga agttaaagaa
    11821 gtgttgacca aatatttttc tcagaagtta gatgaccgct tgaatgactt atgggattcg
    11881 ggagttttaa accaagaaaa acttgatgaa ctgagaaaaa ttgatgtccg tacacttccg
    11941 aagagatgaa tagaattgta ttagatacaa atagcttgat acagtgcatt tctcccaaaa
    12001 gcaggtaccg taagatttgg gattgctttt tggatggaac gaatcagtta tgcgtttctg
    12061 atgaaattct taatgaatat gaggaaatat tagaacgttt ggcaggtgtg gaaatagcta
    12121 agctggtgat tgatgtcatt ctcaataatc cttatacttt gttctttact ccttattata
    12181 acttcaatct gataaaatca gatccggatg ataataagtt tgtggactgt gcaattgtgg
    12241 ctaatgccaa gtttattgtt acggaagatc atcattttca tatattgaag caatataatt
    12301 tcccaaaagt aagttttata ggtttggatg atttctgtca ttcactatga atatcttctc
    12361 ttttctgtga caaacagaac ccattagttt atgaaaatct tagtgaccgg ctcccgaggg
    12421 tttgtcggcc ggaaccttgt atcccagctg cacaacatcc gtgacgggaa agcccgcaat
    12481 tacggcattg caggtgagga acttaccatt tacgaatatg acatgaacag tgaccctgcc
    12541 gaactggatg tctattgcaa acaggctgac tttgtcttca atctggcagg cgtgaaccgt
    12601 ccccaggatg cgtctgagtt tatgaaaggg aacttcggct tcgcctccac gctgcttgat
    12661 acattgaaga agtacggcaa tacctgtccg gtaatgattt cctcttccac gcaggccgca
    12721 ctcgacaacc cttacggtga atcgaagcgg gcaggggagc agctgttgtt cgagtactcc
    12781 agggaaacgg gtgcaaaggt actggtgtac cgcttcccga atg
//
