GCA_024794045.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaMDIJOJFD_2 (original name was: gnl|Prokka|MDIJOJFD_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	resorcinol	1	34,633

gnlProkkaMDIJOJFD_4 (original name was: gnl|Prokka|MDIJOJFD_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	resorcinol	138,565	179,707
Region 4.2	RRE-containing	3,152,456	3,172,800
Region 4.3	NRPS	5,668,946	5,713,301
Region 4.4	phosphonate	6,092,768	6,107,081	polysaccharide B	Saccharide	13%
Region 4.5	RRE-containing	6,508,445	6,528,729

Region	Type	From	To	Most similar known cluster	Similarity
Region 2.1	resorcinol	1	34,633
Region 4.1	resorcinol	138,565	179,707
Region 4.2	RRE-containing	3,152,456	3,172,800
Region 4.3	NRPS	5,668,946	5,713,301
Region 4.4	phosphonate	6,092,768	6,107,081	polysaccharide B	Saccharide	13%
Region 4.5	RRE-containing	6,508,445	6,528,729

Compact view

gnlProkkaMDIJOJFD_2 - Region 1 - resorcinol

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Location: 1 - 34,633 nt. (total: 34,633 nt) Show pHMM detection rules used

Region on contig edge.

resorcinol: DarB

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaMDIJOJFD_4 - Region 1 - resorcinol

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Location: 138,565 - 179,707 nt. (total: 41,143 nt) Show pHMM detection rules used

resorcinol: DarB

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaMDIJOJFD_4 - Region 2 - RRE-containing

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Location: 3,152,456 - 3,172,800 nt. (total: 20,345 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaMDIJOJFD_4 - Region 3 - NRPS

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Location: 5,668,946 - 5,713,301 nt. (total: 44,356 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

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NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 3, location 5668945 - 5713301:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg4_4875

AMP-binding (8..447): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYFGSGSLIK
Nearest Stachelhaus code(s):: Ala DMGGFGCLIK (50% 8Å match); Leu DYFTFGLIIK (41% 8Å match); Hpr DYQYCGHLIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg4_4876

AMP-binding (607..1026): X

nrpys: Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMTNMGEVAK
Nearest Stachelhaus code(s):: Asn DMTKVGEVGK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

gnlProkkaMDIJOJFD_4 - Region 4 - phosphonate

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Location: 6,092,768 - 6,107,081 nt. (total: 14,314 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaMDIJOJFD_4 - Region 5 - RRE-containing

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Location: 6,508,445 - 6,528,729 nt. (total: 20,285 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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