LOCUS       c00021_gnlPro..         8114 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FKAABEMD_24.
ACCESSION   c00021_gnlPro..
VERSION     c00021_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 15:25:30
            Original ID  :: gnl|Prokka|FKAABEMD_24
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8114
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8114
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2708:5825]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2709..5825
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8114
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8114
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(192..1112)
                     /locus_tag="ctg21_1"
                     /transl_table=11
                     /translation="LKTINLGHSNIQASSVALGIMRMDALDVAQATKVIDTAADLGINY
                     VDSADIYGGGQSSTIFGQALKQAHVSRDQLYIQSKGGIVPGKRYDFSKQHILAAVDGEL
                     QRLGVDYLDTFLLHRPDALMEPEEVAAAFDKLQTSGKVRHFGVSNFNPMQVDMLQAALN
                     QRLIINQLQFGIMHTGAIDFGLHTNMQDERSLNHDGEMIEYSRLHKMTIQAWSPYQYGN
                     FAGIFLDNPKFPELNNAMQALADEKQVTKSAIATAWILRHPAHFQVILGTMNPEHLAQN
                     AAGADVTLSRQEWYDIYFAAGNDLP"
     CDS             2155..2484
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FeS_assembly_P"
                     /gene_functions="other (smcogs) SMCOG1048:Iron-sulfur
                     cluster assembly protein (Score: 66.9; E-value: 4.7e-21)"
                     /gene_kind="other"
                     /locus_tag="ctg21_2"
                     /sec_met_domain="FeS_assembly_P (E-value: 5.3e-22,
                     bitscore: 66.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGRDTMTTTFKDRAFEALGHVIDPELGIDLVNLGLIYDAQLTAE
                     GTAEITMTLTIAGCPLTEWLAKDIHDELIKLPEVNNIEIEIVWEPAWNESMMSREARME
                     LGIHS"
     CDS             complement(2508..2693)
                     /locus_tag="ctg21_3"
                     /transl_table=11
                     /translation="MTKTPAIQSSTDGCVTGVFLLARRIHVLLAGISTAARKKGAASLN
                     QQRLSLIMPYPMTVSP"
     CDS             complement(2709..3530)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74; E-value:
                     4.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-28, bitscore:
                     88.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCH
                     MRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAFWGKTGGITVSGGESLVQIDFILDL
                     FEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLTQYG
                     NENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYH
                     TLGVKKYHEMKIKYRLEGIESPTQDRVANAEKLLHTADYNGYKTWMPLPKL"
     CDS             complement(3567..5825)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_5"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-40, bitscore:
                     126.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.8e-247, bitscore:
                     810.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFL
                     AGPTAATKTLNDKVLELKKQERAAGGVLDADTKVVATITSHGPGYIQKDLEKIVGLQTD
                     KPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRKARH
                     YKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQ
                     DQYRALADMKKMAASYGYDISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTM
                     DIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEEYNSLFSGDPIWATLSMCGLG
                     VDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEN
                     DDLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGP
                     AYEPITSEYIDYDEFMKKLDKQMDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLD
                     RTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADNDYPRYGNNDDRADDIAK
                     WLVKELYSKMDTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAY
                     GAEKSGALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKND
                     GMHLNINVFNKDTLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVISRTFFESM"
     CDS             6243..7232
                     /locus_tag="ctg21_6"
                     /transl_table=11
                     /translation="MRMLEKIEQYIDSCIEAGDIYGASFSLITPKGINQYYHGKQGRDE
                     FAVGLDPAMIYDLASVTKVVGTTTRIFQLLSDHTIHLTDSVARFLPGFTHPEITIKNLL
                     LHNSGLPADIDNLDKMQREDLIKAVYDAPLVTAPGTTYVYSDLGFILLGWIIRAVDGSL
                     ARSIQDHVCYPLAMTNTGYNLNRPKIRFVPTEFDPRRGQIQGQVHDYKAFLLNGESGHA
                     GLFSTLTDLSVFIEMMLNFGEYQGKRVLDENVFDWLGEYDENGRTLGWERRAGRHQYFH
                     TGFTGPAVAFDLDRQIGLVVLTNRVYPTSDNQVWNQDRKRVFDLFFES"
     CDS             complement(7400..7915)
                     /locus_tag="ctg21_7"
                     /transl_table=11
                     /translation="MLAWLPFFLISLMFIVLLYACARSARTNIQRFKQLSLIAFLWVLT
                     AFCYTPTSYNFGTQLVIQYIQWGPVKLNVIPFQQLDIEFWLNVLLTVPLGGLLAWNFPD
                     LPWRRFIWLGFLTGLTLELGQFILDWLVNLKRWVDIDDVITNWAGVIIGAAGYKIARHV
                     PGLRWLQK"
ORIGIN
        1 cagctcctaa aagatgggtt tgtggtaaac accattttaa aggaagctga tcttttttgt
       61 ccgaacagcg ttcggattaa ttttacaatc taccaaacgt ctaaaaaacg acgacctgaa
      121 aatcaatcct gatttggggt cgtcgttttg attgtaaaaa ctaatcgtca attctgttag
      181 gtcgatcgtg attaaggtaa atcgttgcca gctgcaaagt aaatgtcgta ccattcttgc
      241 cgagaaagcg taacgtctgc tccagcagca ttttgagcta gatgttccgg attcatcgta
      301 ccaaggatga cttggaagtg ggctgggtgc cggagaatcc atgcggtcgc aatagctgat
      361 ttagtgacct gtttctcatc ggcgagtgct tgcatggcat tattaagttc tggaaacttc
      421 ggattgtcta agaagatacc cgcgaagtta ccgtattgat aaggcgacca agcttgaatc
      481 gtcatcttat ggagccgtga gtattcgatc atctcgccgt cgtggttaag actacgttcg
      541 tcctgcatgt tcgtgtgcaa gccaaagtcg atcgcaccgg tatgcatgat accaaattgg
      601 agttggttga tgatgagccg ttggtttagt gccgcttgaa gcatgtcaac ttgcatggga
      661 ttgaagttag agacgccaaa gtggcgcacc ttgccgctag tttgcaactt gtcaaaagcg
      721 gcagcaactt cttcgggttc catcagggcg tccggtcgat ggaggaggaa ggtgtcgaga
      781 taatcaacac ctaagcgttg aagctcgcca tccacggctg ccagtatgtg ctgtttggaa
      841 aagtcgtaac gcttgccagg aacaatgccg ccttttgact gaatatagag ctgatcacgc
      901 gatacgtgag cctgcttgag tgcttgaccg aagatggtcg atgattgacc accaccgtag
      961 atatcagctg aatcaacgta attgatgcct aaatcagcag cggtatcgat aaccttggtg
     1021 gcttgagcga catccaaggc gtccatgcgc atgatgccaa gcgccacgct ggaagcttga
     1081 atattgctgt gacctaaatt aatcgttttc aaaactaaag tcctcctcaa tgtgcaactt
     1141 gatttaaatt cagtatacgg ttttcgtaag aggatgtaaa cggttacgtc tggtgccgac
     1201 tgttggtaaa tctgatttta gagcaaaaaa atagaaccca aggcttctct gcttgagttc
     1261 tacttatgag gaataatcct gattagcaat gaaaaattgt tcagtgttaa tatgatacac
     1321 cttcatgcta ttcaatttta cgcgtgtcca acttctaacg gactaacccg tatttattgt
     1381 aactatctgc tatagtcgat ctaaagtaag gttgttactt gattgcgact cgttagacgg
     1441 gttttaacga aagtcaaact cgcgtttaaa acttgggaat gtcttcgtaa cagacatgca
     1501 cctccgttta aactttgtaa tacctgagtc tacaaatgga actggtaatg tggaactaac
     1561 aactggtcat cggagacacc tgtttccaac taaaacttag ttaaaatctg ttcgtcattc
     1621 taatgccaat taattctttg cggttatcgt ttatctaagg gtcaacgttc agtcatccaa
     1681 cgtaaatctc acgacactcg ctagaccgat aacgcttcac ggcgcttaaa acggtttcga
     1741 taacgaccca aactgtgata ctagctgtct aacattacag cgacagccaa actcttttga
     1801 gaagctttgc taatcgagga ttaattcctt tcctcactta gtataatagc ataaaccgca
     1861 aacgcttaca acgaaaacaa ccgtgaattc gcaataaaaa tcaatagtga ctcaaaacgc
     1921 tgctatatcg gggattatag tgaatttttt tcgagaaata gtcgcataat ttgctgattg
     1981 accgaccgtt tgaaaagtcg gttaattagc gctaataatt gattgaatat gggctgacat
     2041 acggtacaat agaagtagac aagaaagcgc tttatataac gatgacgtgt atcaagtgtg
     2101 gtggcactta taccgcgaaa cgttggcgtg attaatcagt gaattcaagc tggtatggaa
     2161 ggacgagata cgatgacaac aacgtttaaa gaccgggcct tcgaggcgct aggacatgtc
     2221 attgaccctg aattaggaat tgatctagtt aacttggggt tgatctatga tgcacaattg
     2281 acggcggagg ggaccgctga aattaccatg acgttgacca ttgcgggctg tcctttgacc
     2341 gaatggttgg ctaaggacat tcacgatgaa ctgatcaaat taccagaagt taacaatatc
     2401 gagattgaga ttgtgtggga acctgcttgg aacgaaagta tgatgtcccg cgaagctcga
     2461 atggaactag gcattcatag ttagtggccg gcctgttcgc agctagttca agggctgacg
     2521 gtcattggat agggcatgat taaggaaaga cgttgctggt ttagactcgc ggcgcctttt
     2581 ttgcgtgccg ctgtactaat tccagccagc aatacgtgta tgcgtcgagc cagcaaaaaa
     2641 acaccagtga cacaaccatc cgtagacgat tgtatcgctg gtgttttagt catgctagaa
     2701 ataccgattt aaagttttgg caatggcatc catgtcttgt acccgttgta atcagcagtg
     2761 tgcagtagct tttcggcatt tgccacccga tcttgggttg gagactcgat tccttcaagc
     2821 cggtacttga tcttcatttc atgatatttt ttaacgccca aagtatggta cggcaatact
     2881 tcgacttttt gaacgacgtc gtttggaatt ttagcaatgt aatcgccgag tttcttcaag
     2941 tcttcatcgt aatcagtccg ttggggaacc aggacgtgac gaatccacat atcatcgtgg
     3001 tgttgggcca tgtactgaat catatccaag atattttcgt tcccatactg ggtcaactgc
     3061 ttgtgtttgg cagaatcgat gtgcttaatg tcgaccaacg aaatgtccgt gaccttcatt
     3121 agacgttcga acttgtcaaa gaaaggttgt tctcgggtaa aaggctgtcc agaggtatcc
     3181 agacaagtac tgatattcat cgccttggct ttttcgaata agtctaagat gaagtcgatt
     3241 tgtaccagtg attcaccacc actgactgtg atgccacccg tcttgcccca gaaagcccgg
     3301 tatttagccg cgtcttcgag aatctcgtcg gccgtcattt gatcgccaac gttgagtttc
     3361 caagtatcag ggttgtgaca gtattggcaa cgcatgtggc atccttgaag gaatgccacg
     3421 taacggatac ctggtccgtc aacggagcca aacgtttcga tggagtgaac gtagcctatc
     3481 aaaggctcct ttgccgccgc ttgcgttgtt gaaacttgtt tgttttccat gagcctttcg
     3541 ccttcttaac tattaaagat taaatgttac attgattcga agaatgtccg tgaaataacg
     3601 tcatcttgtt gttccttggt taaatctgcg aagtagacac agtagccaga aacccgaacc
     3661 gttaatgttg ggtattcttc agggtgtttc tgagcatcaa tcaaagtgtc tttattgaag
     3721 acgttgatgt tcaagtgcat cccatcgttc ttcatgtaac cgtctaacat gtttactaac
     3781 gtgtctttcc gtgattcgag gtcatggcct aacgtgttag gggtaacgcc gaacgtgttg
     3841 gaaatcccgt cagttgcgta acggtatggt aatttggcag ttgaaagaag tgaagctaat
     3901 gcaccactct tttcagcacc gtatgcaggg ttagcacctg gtgagaatgg ttcgcctttt
     3961 tgacggccat ttggtgtggt accagtgttc ttaccataaa caacgttgga ggtaatcgtc
     4021 aaaacagaag ttgaaagttt ggcattccga tagaggtgat gcgtgtccat cttgctgtat
     4081 aattctttga ctaaccattt agcaatgtca tcagcgcgat cgtcattgtt cccataacgt
     4141 gggtagtcat tgtcggcttt gaagtcaacg gcgataccac gttcgtcacg aattacttta
     4201 acgtgaccat acttgatagc tgagattgaa tccgcggcat gtgataagcc agaaatccca
     4261 gttgcaaagg tccgatcaag atcagtattc ttcaaagcta attgggcagc ttcatagtag
     4321 tacttatcat gcatataatg aattgcattc agtgagttaa cgtaagtgtc agctaaccaa
     4381 tccatttgct tgtctaattt cttcatgaat tcgtcgtaat cgatgtattc tgaagtaatt
     4441 ggttcgtaag cagggccaac ttgatctcca gcaatttcgt cgcggccacc attgatggca
     4501 taaagaatcg ctttggctaa gtttgcccgg gcaccgaagt actggattcc atcagcaatt
     4561 ggttgtgcgg aaacacagca agcaatgcca taataatcgg taccccattg tacccgcatc
     4621 aagtcatcat tttcatactg aatggttgaa ctgtcgattg aaacttcagt tgcgtaacgt
     4681 ttgaagtctt ctggtaagcg gtctgaccat aaaatcgtga tgtttggttc tggtgcggcg
     4741 cccatgttgt ccaaagtctt taaaatccgg aaagcggtct tagtcacatg gtgttgaccg
     4801 tcgacgccta aaccacacat tgataaggtt gcccagattg ggtcaccaga gaagagagaa
     4861 ttgtattctt cagtacggat gaaccgaacc atccgtagtt tcatgacgaa ttgatcaatt
     4921 aattcttgag cttggctttc gtccagaaca ccattgtcca agtcacgttg gatgaagatg
     4981 tccatcgttg tatcaatccg gccaacggac attgcagcgc cgttttgggt cttgatagca
     5041 gctaagtaag cgaagtacat ccattgaata gcttcttgag cattagttgc aggcttgcta
     5101 atatcgtagc cataactggc agccatcttc ttcatatctg ctaaagcacg gtattgatct
     5161 tgaacttctt cacggaggcg aataacatca tcagtcaatt caccatcacc aatgtgggca
     5221 aagtcattag ctttgtcttc cattaaacga tcgatcccat aaacagcgac ccgtggaaga
     5281 tcaggaatga gacggccacg tgcgtatgca tctggtagtc cggtgatgat cttgtagtga
     5341 cgtgctttcc gcatgtcagg agtataaaca tcgaagacgc cttggttatg agtcttgcga
     5401 tattcagtga aaatcttgtc gttttcttca tcaggggtat aaccgtatga tttcaaagcg
     5461 tcatcagcca ttcgaatacc accaaatggc atgaaggccc gcttcaaagg cttgtcagtc
     5521 tggagaccaa caatcttttc gagatctttt tgaatataac cagggccgtg tgaagtaatc
     5581 gttgcaacga ctttagtatc agcgtccaac acaccaccag cggcacgttc ttgtttcttt
     5641 aattctaaga ctttgtcatt caaggtctta gtagcggctg ttggtccggc caggaagctt
     5701 tcgtcgccat tgtactgtgt gaagttattt tgaataaaat cacgaatgtt gatttcttcc
     5761 tgccagtgcc cgcctttgaa acctttccaa gctgctggtt ttgttgtctt ttctgatgta
     5821 atcattttat cgcctccata atttgaatcc aatcaaatga ttgataaaat tctatatttg
     5881 aatcataaca ttttttgctg gatttgtaac cgctttcagg tgatttattt cacgtttggg
     5941 ttattttttt gcaatctatt attgaaaagt tgactattaa cgttgatata acagtactta
     6001 catagctaga taggcattgg taattcactt aattatattt agaaaacggc taaaaacaag
     6061 acttgttttg atatataagt ggaatattgt ccataatgta acaagtgttt aactagttaa
     6121 ttaataaaaa gtgcaacgag gtgtggaggg tgttaaaagc gtttgccgtt gattttatga
     6181 ggaagttcgt tatcataaag aagataagca gaaactagtg aatgtagtga aaggtgggcg
     6241 taatgcggat gctcgaaaaa attgaacaat atattgacag ttgtattgaa gctggcgaca
     6301 tttatggggc gtcgtttagc ttgatcaccc ctaaaggtat caatcaatac tatcatggta
     6361 agcagggccg cgatgagttt gccgtgggct tagatcctgc gatgatttat gacctagcat
     6421 cggtaactaa ggttgtaggg accacgacga ggatctttca gttgttatcg gaccacacga
     6481 ttcatttaac ggactcggtt gcccggtttt tacctgggtt cactcatcct gaaataacga
     6541 ttaaaaactt gttacttcat aatagtgggc taccggcaga tattgataac ctggataaga
     6601 tgcaacgtga agatctgatc aaagcggttt atgatgcgcc acttgtcacg gcaccaggga
     6661 caacgtatgt ctattcagac ctgggattta ttttattggg atggattatt cgtgcggttg
     6721 acggctcatt ggctcggagt attcaagacc acgtttgcta tccactagcg atgaccaata
     6781 cgggttataa cttaaatcgt ccgaagattc ggtttgtgcc gacggagttt gatccgcggc
     6841 ggggtcagat tcaaggacag gtgcatgatt ataaagcctt tttgttgaat ggtgagagtg
     6901 gtcacgcagg cttgttctca acgctgacgg atttatccgt atttattgaa atgatgttaa
     6961 actttggtga atatcaaggc aaacgggtgc tggatgaaaa tgtctttgac tggttaggcg
     7021 agtatgacga aaatggccgg acgttaggtt gggaacgacg tgcgggacga catcaatatt
     7081 tccatactgg ttttactggc ccggcagttg cttttgattt ggatcgtcaa attgggctgg
     7141 ttgtgttaac aaatcgagtt tatccaacta gtgacaatca ggtctggaac caagatcgta
     7201 agcgagtatt cgatctgttt tttgaatcct gatgatggca gcttcattaa tagttagtag
     7261 ttaaattaaa taagcgccgc agccacttaa ggctgcgacg cttatttaat tacgatttca
     7321 tgacagctag accgctgaaa tgtttggcac ttgtgggtgc taaacaaacg ctattttgct
     7381 ttttgcttct atatatacgt tatttttgca accagcgaag tccgggaaca tgacgtgcaa
     7441 ttttataacc agcggcaccg ataatgacac cagcccaatt cgtgatgaca tcatcgatat
     7501 caacccaacg tttgaggtta accaaccagt caaggatgaa ttgtcccagc tcgagtgtta
     7561 gccccgtaag aaatcccaac caaataaaac gacgccaagg caagtcggga aaattccatg
     7621 ccagtaaacc gcccaatgga acagtgagga gaacgttgag ccaaaattca atgtcaagtt
     7681 gttggaaagg aatcacgttc agcttaacgg gtccccattg aatatattga ataacgagtt
     7741 gggtaccaaa attgtaactg gtaggtgtat agcaaaaagc agttaagacc cagagaaagg
     7801 caattaaact gagttgctta aaacgctgga tatttgtgcg cgctgatcgc gcacaagcgt
     7861 atagtagaac aataaacatt aatgaaatca aaaagaatgg tagccaagct agcataattt
     7921 agtcgctcct ttaattaaat agtatagaga aagaaagctg gtttgactat taataaaaag
     7981 aaattcagaa aaagtgttga cactttttac ttcaattggt atattagtaa acgttgctgc
     8041 tacggcagat gcatctcacc accaaataat tattgaaatt agttgttgac aacttcttca
     8101 gtaattggta agat
//
