GCA_026123105.1 - 13 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaEIJMBHOK_1 (original name was: gnl|Prokka|EIJMBHOK_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	PKS-like	66,639	107,883	butirosin A/butirosin B	Saccharide	7%
Region 1.2	terpene	189,885	210,625
Region 1.3	transAT-PKS	868,062	972,827	difficidin	Polyketide	100%
Region 1.4	T3PKS	1,088,278	1,129,378
Region 1.5	terpene	1,192,866	1,214,749
Region 1.6	NRPS,betalactone,transAT-PKS	1,239,137	1,363,456	fengycin	NRP	100%
Region 1.7	transAT-PKS,NRPS,T3PKS	1,417,321	1,527,441	bacillaene	Polyketide+NRP	100%
Region 1.8	transAT-PKS	1,750,068	1,838,286	macrolactin H	Polyketide	100%

gnlProkkaEIJMBHOK_2 (original name was: gnl|Prokka|EIJMBHOK_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	1	12,690	fengycin	NRP	20%

gnlProkkaEIJMBHOK_11 (original name was: gnl|Prokka|EIJMBHOK_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	other	303,107	344,525	bacilysin	Other	100%
Region 3.2	RiPP-like,NRP-metallophore,NRPS	872,939	924,733	bacillibactin	NRP	100%

gnlProkkaEIJMBHOK_12 (original name was: gnl|Prokka|EIJMBHOK_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	lanthipeptide-class-iii	21,437	44,052	andalusicin A/andalusicin B	RiPP:Lanthipeptide	100%
Region 4.2	NRPS	139,149	203,654	surfactin	NRP:Lipopeptide	82%

Compact view

gnlProkkaEIJMBHOK_1 - Region 1 - PKS-like

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Location: 66,639 - 107,883 nt. (total: 41,245 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)

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NRPS/PKS domains

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaEIJMBHOK_1 - Region 2 - terpene

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Location: 189,885 - 210,625 nt. (total: 20,741 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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No matches found.

gnlProkkaEIJMBHOK_1 - Region 3 - transAT-PKS

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Location: 868,062 - 972,827 nt. (total: 104,766 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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core biosynthetic genes

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 3, location 868061 - 972827:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - mal - mal) + (ohmal - mal) + (mal) + (ccmal - ccmal - ohmal - ccmal) + (ohmal - mal) + (Me-ccmal) + (mal)
Non-colinear ordering:: ctg1_932 + ctg1_933 + ctg1_934 + ctg1_935 + ctg1_936 + ctg1_937 + ctg1_938

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_927

PKS_AT (4..281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 86.6
inactive: 30.5
Methoxymalonyl-CoA: 28.9
Methylmalonyl-CoA: 21.4
Propionyl-CoA: 16.1
2-Methylbutyryl-CoA: 14.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg1_930

AMP-binding (15..363): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IGNPTGTIFK
Nearest Stachelhaus code(s):: 2,3-dohHda IGNPTGTIMK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaEIJMBHOK_1 - Region 4 - T3PKS

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Location: 1,088,278 - 1,129,378 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaEIJMBHOK_1 - Region 5 - terpene

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Location: 1,192,866 - 1,214,749 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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gnlProkkaEIJMBHOK_1 - Region 6 - NRPS,betalactone,transAT-PKS

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Location: 1,239,137 - 1,363,456 nt. (total: 124,320 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 6, location 1239136 - 1363456:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Orn) + (Tyr - D-Thr) + (D-Tyr) + (Ile) + (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 1239136 - 1303283:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Orn) + (Tyr - D-Thr) + (D-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 1286200 - 1363456:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_1289

AMP-binding (463..746): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFG---K
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (56% 8Å match); Gln DAWHFGSVEK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg1_1290

Search NORINE for peptide: strict or relaxed

AMP-binding (263..668): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEVGSIDK
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1291

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTLTAEVAK
Nearest Stachelhaus code(s):: Tyr DGTLTAEVAK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1509..1905): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1292

AMP-binding (468..751): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFG---K
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (56% 8Å match); Gln DAWHFGSVEK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg1_1293

Search NORINE for peptide: strict or relaxed

AMP-binding (299..691): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code(s):: Tyr DGTITAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1294

Search NORINE for peptide: strict or relaxed

AMP-binding (473..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code(s):: Ile DAFFYGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1298

AMP-binding (12..445): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -GLHIAAPVK
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (56% 8Å match); Ile DGLFIGIPVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg1_1317

PKS_AT (6..290): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.4
inactive: 30.7
Methoxymalonyl-CoA: 20.8
Propionyl-CoA: 12.4
Methylmalonyl-CoA: 11.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_1318

Search NORINE for peptide: strict or relaxed

CAL_domain (36..478): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 616.7
NH2: 18.0
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2956..3351): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1319

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code(s):: Tyr DALSVGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1779..2174): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3286..3683): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4325..4732): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1320

Search NORINE for peptide: strict or relaxed

AMP-binding (267..674): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1791..2190): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaEIJMBHOK_1 - Region 7 - NRPS,T3PKS,transAT-PKS

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Location: 1,417,321 - 1,527,441 nt. (total: 110,121 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T3PKS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 9, location 1417320 - 1527441:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_1422

Search NORINE for peptide: strict or relaxed

AMP-binding (492..896): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNMAIIYK
Nearest Stachelhaus code(s):: Ala DVSNMAIIYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1425

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 27.0
NH2: 9.4
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_1430

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.5
inactive: 28.1
Methoxymalonyl-CoA: 23.4
Methylmalonyl-CoA: 11.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_1431

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 37.3
inactive: 27.1
Methoxymalonyl-CoA: 19.8
Benzoyl-CoA: 11.5
Methylmalonyl-CoA: 9.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_1432

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.5
inactive: 47.1
Methoxymalonyl-CoA: 40.1
Methylmalonyl-CoA: 25.3
Propionyl-CoA: 23.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaEIJMBHOK_1 - Region 8 - transAT-PKS

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Location: 1,750,068 - 1,838,286 nt. (total: 88,219 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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NRPS/PKS domains

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 10, location 1750067 - 1838286:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal) + (ccmal) + (ccmal - mal) + (ccmal - mal) + (ohmal) + (ohmal - mal)
Non-colinear ordering:: ctg1_1688 + ctg1_1687 + ctg1_1686 + ctg1_1685 + ctg1_1684 + ctg1_1683 + ctg1_1682

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_1689

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 93.5
inactive: 32.0
Methoxymalonyl-CoA: 28.9
Methylmalonyl-CoA: 20.9
Propionyl-CoA: 17.3
fatty_acid: 13.9
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaEIJMBHOK_2 - Region 1 - NRPS

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Location: 1 - 12,690 nt. (total: 12,690 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 12690:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X) + (Pro - Gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg2_1

AMP-binding (623..1016): X

nrpys: Ala, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWIGGTFK
Nearest Stachelhaus code(s):: Ala or Val DVFWIGGTFK (97% 8Å match); Val DVFWIGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg2_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..866): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1500..1901): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGSVEK
Nearest Stachelhaus code(s):: Gln DAWHFGSVEK (100% 8Å match); Glu DAWHFGSVEK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaEIJMBHOK_11 - Region 1 - other

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Location: 303,107 - 344,525 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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gnlProkkaEIJMBHOK_11 - Region 2 - NRP-metallophore,NRPS,RiPP-like

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Location: 872,939 - 924,733 nt. (total: 51,795 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 872938 - 924733:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 872996 - 924733:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg3_900

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (97% 8Å match); diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg3_902

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaEIJMBHOK_12 - Region 1 - lanthipeptide-class-iii

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Location: 21,437 - 44,052 nt. (total: 22,616 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

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Lanthipeptide predictions

Lanthipeptide(s) for ctg4_27

ctg4_28

Leader:

MNDVLELQKLEKETDGKGIAV

Core:

DATITTTWTVTTTSGFVSSVSNHC

Core with dehydrations:

DADhbIDhbDhbDhbWDhbVDhbDhbDhbDhaGFVDhaDhaVDhaNHCys

MIBiG 3.1 matches for ctg4_28

37.5% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

9

TVTTTSGFVSSVSNHC

24

TVTTT + S++SN+C

8

TVTTTGAWASTISNNC

23

37.5% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

TVTTTSGFVSSVSNHC

24

TVTTT + S++SN+C

8

TVTTTGVWASTISNNC

23

antiSMASH-DB 4.0 matches for ctg4_28

100.0% NC_022081.1and 19 others:

NZ_CP016913.1 Lanthipeptides: BARD7_RS19060_lanthipeptideNZ_CP022654.2 Lanthipeptides: CHN56_RS21570_lanthipeptideNZ_CP032144.1 Lanthipeptides: D3C60_RS19850_lanthipeptideNZ_CP034203.1 Lanthipeptides: EBA29_RS19705_lanthipeptideNZ_CP040378.1 Lanthipeptides: D1120_RS19620_lanthipeptideNZ_CP068563.1 Lanthipeptides: JKJ03_RS01160_lanthipeptideNZ_CP069391.1 Lanthipeptides: JR311_RS02265_lanthipeptideNZ_CP073635.1 Lanthipeptides: KEF49_RS01155_lanthipeptideNZ_CP083764.1 Lanthipeptides: LAZ98_RS01175_lanthipeptideNZ_CP085388.1 Lanthipeptides: LIS75_RS01165_lanthipeptideNZ_CP088300.1 Lanthipeptides: LQ763_RS01075_lanthipeptideNZ_CP097586.1 Lanthipeptides: M8971_RS04245_lanthipeptideNZ_CP107556.1 Lanthipeptides: OF857_RS01165_lanthipeptideNZ_CP116014.1 Lanthipeptides: PF996_RS01170_lanthipeptideNZ_JAGFPJ010000003.1 Lanthipeptides: J5M77_RS15590_lanthipeptideNZ_JAQEMJ010000010.1 Lanthipeptides: PGW53_RS13095_lanthipeptideNZ_LMAG01000010.1 Lanthipeptides: ASQ47_RS20350_lanthipeptideNZ_PVRP01000003.1 Lanthipeptides: C6355_RS19570_lanthipeptideNZ_SUKE01000008.1 Lanthipeptides: FAZ22_RS20105_lanthipeptide

Lanthipeptides: BASU_RS20300_lanthipeptide

1

DATITTTWTVTTTSGFVSSVSNHC

24

DATITTTWTVTTTSGFVSSVSNHC

1

DATITTTWTVTTTSGFVSSVSNHC

24

95.8% NZ_JALAQD010000016.1and 1 other:

NZ_JALAUL010000008.1 Lanthipeptides: MOE42_RS14800_lanthipeptide

Lanthipeptides: MOD08_RS18745_lanthipeptide

2

ATITTTWTVTTTSGFVSSVSNHC

24

ATITTTWTVTTTSGFVSSVSNHC

1

ATITTTWTVTTTSGFVSSVSNHC

23

50.0% NZ_FOHE01000012.1and 1 other:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20540_lanthipeptide

Lanthipeptides: BMX40_RS20545_lanthipeptide

7

TWTVTTTSGFVSSVSNHC

24

TWTVT F+S+VSNHC

4

TWTVTI---FLSTVSNHC

18

with 10 more sequence matches

37.9% NZ_CP089924.1: Lanthipeptides: LVB77_RS02735_lanthipeptide

9

TVTTTSGFVSSVSNHC

24

+V TSG SSVSNHC

5

SVNCTSG--SSVSNHC

18

37.5% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

5

TTTWTVTTTSGFVSSVSNHC

24

T WT+ TT S++SN C

1

TAGWTLLTTLIGTSTISNSC

20

34.5% NZ_CP025227.1: Lanthipeptides: allorf_0999194_0999376_lanthipeptide

8

WTVTTTS--GFVSSVSNHC

24

WTV TTS G V ++S C

2

WTVPTTSTIGLVCTLSWEC

20

33.3% NZ_WEHU01000003.1and 3 others:

NZ_WEHU01000003.1 Lanthipeptides: F9279_RS25000_lanthipeptideNZ_WEHU01000003.1 Lanthipeptides: F9279_RS24995_lanthipeptideNZ_WEHU01000003.1 Lanthipeptides: F9279_RS24990_lanthipeptide

Lanthipeptides: F9279_RS25005_lanthipeptide

16

FVSSVSNHC

24

FVSS+SNHC

15

FVSSISNHC

23

25.0% NZ_BBYG01000022.1: Lanthipeptides: AOQ37_RS40945_lanthipeptide

18

SSVSNHC

24

SS+SNHC

4

SSISNHC

10

25.0% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

16

FVSSVSNHC

24

F S++SNHC

15

FASTISNHC

23

25.0% NZ_CP018933.1and 24 others:

NZ_CP018933.1 Lanthipeptides: BEI49_RS29525_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29520_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29515_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29510_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29505_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29500_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29495_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29490_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29485_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29480_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29475_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04325_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04320_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04315_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04310_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04305_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04300_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04295_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04290_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04285_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04280_lanthipeptideNZ_NTVN01000027.1 Lanthipeptides: CN415_RS28750_lanthipeptideNZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31080_lanthipeptideNZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31075_lanthipeptide

Lanthipeptides: BEI49_RS29530_lanthipeptide

16

FVSSVSNHC

24

+ S+VSNHC

14

WASTVSNHC

22

25.0% NZ_GL545262.1: Lanthipeptides: HMPREF0798_RS11830_lanthipeptide

9

TVTTTSGFVSSVSNHC

24

+++T F+S++S HC

1

SISTLVAFLSTISQHC

16

21.2% NZ_MIFN01000022.1and 1 other:

NZ_MIFN01000022.1 Lanthipeptides: BHL26_RS15355_lanthipeptide

Lanthipeptides: BHL26_RS15355_lanthipeptide

4

ITTTWTVTTTSGFVSS

19

+ TT +T SG++SS

6

LATTGALTAVSGYISS

21

17.4% NZ_QVFA01000004.1: Lanthipeptides: DW882_RS14120_lanthipeptide

5

TTTWTVTTTSGFVSSVSN

22

TTT ++ S VS++SN

14

TTTAIISAISSVVSAISN

31

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg4_28 - Class III

Cleavage pHMM score: 3.30

RODEO score: 0

Monoisotopic mass: 2299.2 Da

Molecular weight: 2300.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2318.5 Da N = 1; 2336.5 Da N = 2; 2354.5 Da N = 3; 2372.5 Da N = 4; 2390.6 Da N = 5; 2408.6 Da N = 6; 2426.6 Da N = 7; 2444.6 Da N = 8; 2462.6 Da N = 9; 2480.7 Da N = 10; 2498.7 Da N = 11

gnlProkkaEIJMBHOK_12 - Region 2 - NRPS

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Location: 139,149 - 203,654 nt. (total: 64,506 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 139148 - 203654:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - Leu) + (D-Leu) + (Val - Asp) + (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg4_154

Search NORINE for peptide: strict or relaxed

AMP-binding (465..867): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1511..1908): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code(s):: Leu DAFMMGMVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg4_155

Search NORINE for peptide: strict or relaxed

AMP-binding (1..339): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg4_156

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1502..1904): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg4_158

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code(s):: Leu DAFFLGCVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)