LOCUS       gnlProkkaNFBAHKHO_54    5380 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NFBAHKHO_54.
ACCESSION   gnlProkkaNFBAHKHO_54
VERSION     gnlProkkaNFBAHKHO_54
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 13:22:25
            Original ID  :: gnl|Prokka|NFBAHKHO_54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5380
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1125
                     /locus_tag="ctg54_1"
                     /transl_table=11
                     /translation="MYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALL
                     GFALLPVPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTL
                     GQFVMDNLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDR
                     RKFLRQFFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFVL
                     VLMFLIVFVRNNRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGY
                     IPFTYLIERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTA
                     LFSGFVSVLSIFAIYCYFKQQLNSLILIPSEIHD"
     protocluster    1..5380
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1138:5379]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1139..5379
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5380
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5380
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             1139..1837
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_2"
                     /sec_met_domain="NTP_transf_3 (E-value: 5.8e-15, bitscore:
                     44.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKNALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             1857..3158
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_3"
                     /sec_met_domain="CTP_transf_like (E-value: 6.4e-13,
                     bitscore: 37.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.6e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.3e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVFEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             3170..4306
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 71.8;
                     E-value: 7.9e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_4"
                     /sec_met_domain="TPP_enzyme_N (E-value: 7.5e-23, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2e-21, bitscore:
                     64.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGVALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             4303..5379
                     /NRPS_PKS="Domain: Aminotran_5 (30-293). E-value: 5.9e-34.
                     Score: 109.4. Matches aSDomain:
                     nrpspksdomains_ctg54_5_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.6; E-value:
                     1.8e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_5"
                     /sec_met_domain="Aminotran_5 (E-value: 3.7e-32, bitscore:
                     100.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVM"
     aSDomain        4393..5181
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg54_5_Aminotran_5.1"
                     /evalue="5.90E-34"
                     /label="ctg54_5_Aminotran_5.1"
                     /locus_tag="ctg54_5"
                     /protein_end="293"
                     /protein_start="30"
                     /score="109.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIK"
ORIGIN
        1 ctgtatgtcc tttctgagat tatcggctac gccctttcca agttcatcgg tgttcgtatt
       61 ctgccttcga tgaaagcggg tcaccgcatc tattatataa tagggctgct tactttttcc
      121 gaagttgctt tgttgggttt tgcacttctt ccggttcctt tgaaggtgtg ttctattttt
      181 ctgagcggtc ttcccctggg tatgatctgg ggtgtcattt ttagctatat cgaaggacgt
      241 cgtatcagtg aaatattgaa tgtcgggctg agcgttgctt tgatagtctc ttccggtttg
      301 gtgaaaacct tgggacaatt cgtgatggat aatttgcatg tgtcggaata ttggatgcct
      361 tttaccaccg gtttgttggt ataccctgtt atgttgcttt gcagctggct tctcaatcag
      421 attccggctc ccaatgagca agatatcata cagcgtacca aacgtgctcc gatggatggt
      481 aaagacaggc gtaaattttt gaggcaattc ttctggggaa tctgcatgct ggttgttttt
      541 tacggtgccc tgaccgtgtt ccgtgagctt cgtgactctt tcgctgccga tatttggaag
      601 gagctccata ttgaaggtgc aatgatcttt actcagacag aggttcccat tgccgttttt
      661 gtccttgtac tgatgttcct gattgtgttt gtccgtaata accgtttggc tcttaatatc
      721 atttattgca ttgccgttac aggcggttcc ttaatggttt tttccacatt gctttatgtg
      781 tacggttttc tttctcctat ttggtggatg attctttccg gtcttggtct gtatatgggg
      841 tatattcctt tcacttacct gatagaacgc ctgattgctt cccttagggt ggttagtacg
      901 gctgttttta ttatttattt ggccgattct ttcggctatc tgggcactac gggtgtgttt
      961 atggtcaaga acttcgtttc tgctgacatt tcttggactg tcatgctaat gcgtactgcc
     1021 cttttttcag gatttgtttc tgtcctgtcg atttttgcga tttactgtta tttcaagcaa
     1081 caattgaatt cacttatatt aattccctct gaaattcatg attaaaacag caatgatcat
     1141 ggctgccggt cttggtaccc gcttcgggca ttataccgag cttgtgccca aaggttttgt
     1201 ggaggttggt ggaaaaccta tgattatccg ctctatcgaa acccttcttt cctgcggtat
     1261 cgaacgtatc atcctcggta cgggttataa aaaagaggct tatgaagccc ttcaggcgga
     1321 tttcccccag atagagacct gtttcagtcc ccgttatgcc gatactaaca gtatgtacac
     1381 tctgtacaat acgcgtgacg tgataggcga tgacagtttc ctgctgcttg aatcggacct
     1441 tgtttttgaa cgcaaggcca tcctctcgct actggatgac gaattcccgg atgtgatgct
     1501 tgtctcttct ctgaccaagt tccaggatca gtattatgtg gaatatgacc gcaatcacat
     1561 ccttacttcc tgttcggtag acaagaacgc ccttgaggca aaaggcgagc tggtcggtat
     1621 ccataaactt tccaatactt tctaccgccg gatgtgcgcc gattatgcga ccatcctgga
     1681 gtcccagcct aaactgggtt acgagtacga actgctccgc atgtcccgtt ccgtttcccc
     1741 tgtccgtgtg ctcagggtgg agggtcttaa atggtatgaa atcgatgatg aagccgacct
     1801 ttcgtatgcc gaggagcaca tcatccgcta ttgttaaaaa caaatctttt ccatatatga
     1861 acaagaaaat ctatgtcggt atgaccgcgg atatcatgca tccggggctg atccatatta
     1921 ttcatgaagc tactaaatac ggtgatgtca tcgtcggtct gctgacggac aaggccatcg
     1981 ccgaacacaa acgcctccct taccttacct atgatcagcg taaggaggtt gttgagaata
     2041 tcaagggggt ttttgaggtt gttccccagg aggactggag ctatgttccg aatctggaaa
     2101 aactcaagcc tgactatatc atccacggcg acgactggaa aacgggcccg ttgcgcgagg
     2161 tgcgcgaaca ggtttttgag gtgatgaacg cccagggggg caaggtaatc gagatccctt
     2221 atacccgggg catcaactcc tcctcgctcg acaaggatat caaggcgatc gggactaccc
     2281 ctgacgtacg tcttaagtcc ctccgccgcc tgatccaggc caagcctgtg gtccgtatcc
     2341 tggaggctca cgacggtctt tgcggtctga ttattgagaa tcttgaagtc cagaagggtg
     2401 ataagcgcga ggtctttgac ggcatgtggt ccagcagcct gaccgactcc acgagtaagg
     2461 gtaaaccgga tattgaagcg gtcgacctta ccacccgttt acaggacctg aacaatatcc
     2521 ttgaatgtac caccaagcct attatttttg atggagacac gggtggcaag atcgaacact
     2581 ttgtctttac tgtgcgcact ctggaacgtc acggcatttc tgcggtaatc attgaagata
     2641 aagtcggtct gaagaagaat tccctttttg gtactgacgc tatccagact caggatagta
     2701 tcgagggttt ctgcgataag atcagagcag ggaagaatgc ccaggtcacc ggtgacttta
     2761 tgatcattgc gcgtatcgaa agccttattg ccggcaaacc catgagtgac gccctcgaac
     2821 gtgcttatgc ctatgtacag gcgggtgcgg acggcatcat gatccacagc aaggataagt
     2881 cgggtgagga tatccgtgag ttctgccgga ctttccgcaa agaatacgcc catgttccca
     2941 ttgtcgttgt tccgacgact tatgaccatg tgcacgaatc cgaacttcac gaatggggtg
     3001 ccaacgtagt catttacgcc aaccaccttc tccgtgccgc ttacccggcc atgatgaacg
     3061 tggccaggac catccttgaa aacgagcgcg ccgaggaggt ccgtccgctt tgtatgccta
     3121 tcaaggaaat tctggaattg attccgggca ctaaataaat cagacggtta tggtaagtgt
     3181 agaaaagata tacgatacgt tccttcatca cggtgttgac ttctttacgg gtgttcccga
     3241 ttcccttttg aagaatattt gcgcctatat cacggatcat gctccgcgtg gtaagcatat
     3301 catcgccgcc aatgaaggtt ccgcggtggg cattgcctgc ggttattata tggcttctgg
     3361 caacgttcct ttggtttata tgcagaactc cggtctggga aatacggtga atcccctctt
     3421 gtcccttgcg gacgagaaag tgtacagcct tcctcttctt ttgctggtcg gctggcgcgg
     3481 tgagcccggc acaaaagacg aaccccagca taagaagcag ggcgaggtta cccttgatct
     3541 tctgaaagcc atgaaaattc cttttataat tctggatacg gatgaagacg aggccctggc
     3601 gcagatccat gacgcggttg tctcggcgaa gagccggtcg attccccatg cgattgtcat
     3661 ccgtaaagat acgtttggaa aatataaatt gcgtcaggaa gccttgaatg actatcctct
     3721 gtcccgcgag gaggcgctaa tgcaggttgt cgaccacctc ggggacaatg acattgtggt
     3781 ttccaccacg ggcaagctct cccgtgagct tttcgagtac cgtgaatcga aagggcaggg
     3841 gcacgcaagg gatttcctaa ctgtgggttc catggggcac agttcttcca ttgccctggg
     3901 ggttgccctt gagaagcctg accgccgtgt ctactgtctt gacggtgacg gtgcctttat
     3961 catgcacatg ggtgctgtca gcaatatcgg cgatctttct cccaagaatt acaggcacgt
     4021 ccttttcaac aacggtgccc atgaatctgt cggcggtcaa cccacgctcg gtttccgtct
     4081 ggatatcgcc tcgatagcga aaggttgcgg ttatgcgcat gtactgactg cctccgacaa
     4141 ggaagggctt tcgtgtgcgc ttgagaagct gtccggcctg tccggtccgg ttcttcttga
     4201 aattaaggtc cggatcgatt cacgtgatga tctgggtcgc cctacgacta ctccggtaga
     4261 gaataaagag cattttatgg catttttaaa agatacgcat tcatgaatat cgaaagaaag
     4321 atccttttga atccgggtcc cgccaccacg acggatacgg tgaagctggc gcaggttgtt
     4381 cccgatatct gtccgcgtga gaaagagttt gcgggtatga tgaaacagtt acgtgacgac
     4441 ctggtccgcg ttgcgcatgg agacctctcc aaacatacgg ccgtcctgtt ttgcggctcg
     4501 ggtacgatca atatcgatat ctgcctgaac tctcttcttc ctgcggataa gaaagttctg
     4561 gttgtcaata acggtgccta cagcacccgt gcggtggaag tctgcgaata ttacggtctt
     4621 ccccatatcg atttgaagtt ttccgtttac gaacttcccg acctgtcggt aatcgaaaag
     4681 acgctttccg acaatccgga catcgctttg gtctatacca cgcaccatga aacgggtacg
     4741 ggtatcttga accccatccg tgagatcgga aaaatagccc attcgcacgg tgccgttttt
     4801 gttgtcgaca ccacttcctc cctgggtatg attccttttg atatcggaaa ggataatgtt
     4861 gacttttgta tggcttcggc tcagaaaggc cttcaggcca tgacggggct ttcctttatc
     4921 ataggtgatg aatccctgat acgtgcttca agggattatc cgaaacgctc ctactattgt
     4981 aacctctatt tacaatacga ttactttgag aagacgggtg agatgcattt tactcctcct
     5041 gttcaaaccg tttacgctgc ccgtcaggct cttgatgaat attttgcagt aggtgaagag
     5101 gctaaattcg cccgtcacaa gagggtcttt gaagccatcc ataaaggttt gcaagagctt
     5161 ggtcttcagt atgcgatcaa acgtgaatgg cagtcgggtc ttgttgtttc tgtattgtat
     5221 cctgatgatc ccctctggga ttttgataag gttcatgatt actgctatga acgtggcttt
     5281 acgatctacc cgggtaagat ttccacaacg aatacattcc ggctttgtgc gttgggagag
     5341 attgatgtgg aggatattga gaggttcttt gaggtgatga
//
