LOCUS       c00061_gnlPro..         7070 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LNNCGPNK_61.
ACCESSION   c00061_gnlPro..
VERSION     c00061_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 18:04:17
            Original ID  :: gnl|Prokka|LNNCGPNK_61
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7070
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7070
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[173:3165]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      174..3165
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7070
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7070
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(174..926)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.1e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(913..3165)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_2"
                     /sec_met_domain="Gly_radical (E-value: 6.3e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.4e-267, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             complement(4362..4835)
                     /locus_tag="ctg61_3"
                     /transl_table=11
                     /translation="MILQFFAAFIGAAGFGFLFHLQFKHIFPAALGGVLTWVIYVIATI
                     WGFDVFISSLFASAFAAIYSEVIAKIRKAPTTLFLIISVVVLIPGGSLYYTMSYAVQKK
                     WAEAAAYGSQTLQCALGLAIGMSLIWIFHDMARKXXXXASSGYSTIWQGRFTI"
     CDS             complement(4832..5593)
                     /locus_tag="ctg61_4"
                     /transl_table=11
                     /translation="VNPPNAILHELLNLAEIMLTNGAEVSRVEETLNRMGHAYGATQMN
                     VFAITSSIVVTMVFEEGEEYTQTRRITTPVGTDFFKLEQANALSRRCCSSPLSLADLKY
                     EINKISKISPAPYTIYLGSMLGAGGFSVFFGGTILDGAIAVIFAFLLCFLQRHLNSICS
                     NTLILQFFCSLFVGSGICVAAHFFPVIHADKVMIGDIMLLIPGILMTNSIRDILIGDTI
                     SGVMRLVESLLWAGALACGFMCAIWFVGGVL"
     CDS             5658..7010
                     /locus_tag="ctg61_5"
                     /transl_table=11
                     /translation="LSYTWYEVFWFFLLYSFAGWVIGTSGAALKEKKFIDVGFLFGPWC
                     PSYGFGAVGFALFLPELKDQPVFLFAGGVILSFIITSFTGVTLERIFHQKWKDYSRRRF
                     NFGGYVNLPYTVVWGAAALLCIWIVNPFLSKIIGILPYGLGKVILIIAYVIIIIDFIGT
                     VSGILSVHIRLARLGKLVLVEEVADNLQKAADYMGNGLTGWVLKHLEKSHEGLEIKEII
                     RSKLQRREKAQEMTGVFAAGCGFYKLFWLFFLGSFLGDITETIFCWWKMGTITSRSSVV
                     YGPFSLVWGIGCFFLTYMLYQYRDRSDSFIFIFGTVLGGAYEYICSVVLELVFGTVFWD
                     YSKIPFNLGGRINLLYCFFWGIAAVVWMKILYPKLSGFIEKIPKKIGVPLTWFLVVFMV
                     FDMAISGLALNRYHERHQSAKTPETAITKILDTRFPDTRMERIYPSAKHVR"
ORIGIN
        1 ctacctaaaa gggagcatac catttctcta aatcttgcaa aaaatcctgc aaattaatgg
       61 aacaaaatac aaccttagaa gaatccctct gttagaagtc atcgatgcaa tccgaccata
      121 aatatctcca cagctgcgtt tgtcatcaac tttacaaatc agaaattgat agatcagata
      181 gcaagaatct tcttagcgtt ctccactcgt tcttttgttg gtggctcaat tccctccagt
      241 ggatactcta atccaagttc tttccattta taaattccca tcgtatgata aggcaggatt
      301 tccacctttt ccacattatc aagagagtga atgaaatcag ctaatctagt cagatattca
      361 tctttatcgc ttcgttccgg aacaaggaca tgacgaatcc aaattggttt tttgatatca
      421 gaaagatagc gaatcatgtc gagaatattt tcgttgcttc gaccagttaa agtcttgtga
      481 cattcagtat caatatgttt gatatccact aataacaggt ctgtatattt catcaattcc
      541 tgaaatttag aaaagaaagg ttcctctctt gtaaaaggtc ctccagcagt atcaataaca
      601 gtgtggattc ctttttcttt tgccttttta aaaaactcta gcataaaatc aatctgtaaa
      661 agaggctcac caccacttac ggtaataccg ccgctatttt tccagtaagt acgataacgg
      721 agtgcctgat ttaacagctc atccgcactt ctccacaccg caccattttc ctctgtcatc
      781 ttccatgtat ctggattgtg acagaactga catcgcaaag gacaaccctg taaaaaaaca
      841 ataaaacgaa ctcccggccc atccaccgaa ccaaaactct ccgtagaatg aacaagagcc
      901 atcgcatttt tattacattc tgtcatggca tgttctggca attacatcta actgctgttc
      961 ttttgtcaga gagatgaatt taactgcata acctgaaaca cggatcgtaa agtttgcgta
     1021 ttcttctttt tccggatgtt ccatagcatc aatcagtttc tcaacaccaa atacatttac
     1081 gtttaggtgg tgagcaccct gtgcgaagta gccgtccata acatttacta agttattgat
     1141 acgttcttct tcggaatgac cgagtgcatc agggttgatt gtctgtgtat tggaaatacc
     1201 atctaatgcc cattcgtaag gaagttttgc tacagagttt aaggaagcta aaagtccgtt
     1261 ctgctcagca ccataacttg ggtttgctcc tggggaaaga ggttctccgg cctttcttcc
     1321 gtctggaagg gaaccagttg ccttaccata tactacgttt gatgtgatag taaggatgga
     1381 agttgtaggt tcagaatcac ggtaagtatg gaatttttca agcttcttca taaatgtctg
     1441 aagtaaccat acagcgatat catcagcgcg gtcatcatca tttccgtatt tagggaagtc
     1501 gccagttgtc tcataatcaa caacaagtcc ttcttcattg cggactgttt ttactttggc
     1561 atatttaatc gcacttaagg aatctactac atgtgagaat cctgcgatac ctgtagcgaa
     1621 tgtacgacgt acatttgtat cgattaaggc catcaaagca tattcatagt aatatttgtc
     1681 atgcatgtac tggatgagat ttaatgtatt tacatataaa cctgctaacc agtccatcat
     1741 gatatcatat ttgtgcatta cttcatcata gtcaagatat tcggaagtaa tcggtttata
     1801 ttccggtcca acctgctgtc cggtcttttc atccacacca ccattgatag cgtaaaggag
     1861 acattttgca aggttagcgc gagcaccgaa gaactgcatt tccttaccag tctgtgtagc
     1921 agaaacacag cagcagatgg agtaatcatc gccccatact ggacgcatta catcatcatt
     1981 ttcatactgg attgaacttg ttgtaataga aatatgagct gcgtaatctc taaatgcttt
     2041 tggaagacgc tcagagtaaa gtactgtgag gtttggctct ggggatggtc ccatattttc
     2101 taatgtgtgg aggaaacggt agtcagtttt tgtaaccatg gaacgaccgt cctgtccaag
     2161 tccgccaact tcaagtgttg cccatacagg gtcaccggag aagagctggt tgtaagctgg
     2221 gatacgtccg aacttaacca tacggaactt catagtcata tggtcgatta attcctgcgc
     2281 ttctttctct gtaaggattc ccttatccat atcacgtttg atataaatgt caaggaaagt
     2341 agcgatacgt ccgatactca tagcagcacc gttctgtgtc ttgattgctg caaggtatcc
     2401 gaaatacgtc cactgaacag cttcctttgc gttagaagca ggttcggaaa tatcatagcc
     2461 atagctttca gccataactt tcatatcttt taatgcttta atttgcatgg agatttcttc
     2521 tcttaaacgg ataacgtcat ctgtcattgt tccgtcaccg cagtttgcga aatctttttg
     2581 cttgtcagca actaataggt caataccgta aagagctaca cgacggtagt caccaacgat
     2641 acgtccacgt ccgtaagtat ctggaagacc agtaatgatc ttattgtgac gagctttctt
     2701 catttcagga gtataagaat caaatactgc ctggttatgt gtcttatgat aatctgtaaa
     2761 aatcttgtgg aattctggat ttggtgtgta accataagtt gtacaagctt cttcagcagt
     2821 acggatacca ccaaatggca taaatgcacg ttttaaaggt ttatctgtct gaagacctac
     2881 gatctgttct ttctctttta aagactcaga aatgtaaccc ggtttatggg aagtaaggct
     2941 tgatacgata tcagtatcca tatcaagaac accacccttt tcacgctctg ctttctgcag
     3001 cttctgcagt tcgccccaca gtgtgtcagt agcttcggta ggaccttcga ggaaagaagc
     3061 atctccatca tatggagtgt agttatcctg gatgaactct cttaaattaa tttcttctct
     3121 ccagattcgt ccattaaatc cctgccattg ttctttttgt aacatcttaa ttgacctccc
     3181 tgtttgttaa aaaaatcctt aagtcaaggt tttgaaatat gtttgctaaa aaatcaacaa
     3241 aattgttcag atgatttact gtaattagtt ttaacttatt taagttttaa tcatctaaca
     3301 acattattaa catgattttt ggcaatcgtc aaacaatttt ttgatattat gtgttctttt
     3361 ttttgaacat atacacaaaa atgcttgtac agcctttaaa ataaagggtt ttagagcgtt
     3421 gataaaatat tatcaaatcg tacaaatgta ctgatataag tacaataatg acaagcatac
     3481 aactataatt ttatttttcg ctctttagag catgtttgga aaaacatctt ctttagcaca
     3541 tgacttaata acgatttaaa gttacttctt atcagggata ttggatagaa tattatctta
     3601 tcagaaataa aaaagccagc atccagacaa gaattgccca gacggtcggc tataagagtc
     3661 ttatacttca tgtggttcat tccacttact tccgtcagtc gtataagtat cctaactata
     3721 aacaaaatag aacaaaatgt caaggggtta ctatttaatt cattaagaga actacagacg
     3781 aagccattgc ctccaataag gcatatttgt gattttagtc gcggcgcgtt gaatgttcgc
     3841 tccatgcgct cgcatccaac acttgctccg agaccacaaa tatgccttat tggaggcaat
     3901 ggcttctgtg ctatggctgg agaagatgaa ttaaacagag ggaggggatt tggggacaga
     3961 aactgatgtc ttctgcttac tcaagaatga ctgggaacag ggggatgatt tagctttttt
     4021 aatggttatc tcagcaatat ttcaataatt tcagaataaa aagcagactg ttgttatcaa
     4081 tatcaattca ttacctcttc tatttaaata caaaacatct attcacaata ttttaagagc
     4141 aagcagaaaa cttctaccct ctcccccaaa tccccacaac cactatttaa ttcaaacaaa
     4201 atccagccaa agacagcccg ggaagagaac cgatacccta tatttgtggc ctcggagcaa
     4261 ggtttagatg cgagcgcata aagcgagcat ttaaaccgca gcgactaaag tcacaaatat
     4321 agggtatcgg ttctcttccc gggcgtctgt ctcttaatga attaaatagt aaaccttcct
     4381 tgccatatcg tggaatatcc agatgaggct nnnnnnnnnn ccttccttgc catatcgtgg
     4441 aatatccaga tgaggctcat tccaatggca agtcctaaag cacactgtaa tgtttgggag
     4501 ccgtaagctg ctgcttctgc ccattttttc tgaacagcat aactcatcgt gtaatagaga
     4561 gagccgccgg gaattagaac gacaacggaa atgattaaaa ataaggtggt aggggctttt
     4621 cttatttttg cgatgacttc ggagtaaatt gcggcgaaag cactggcaaa tagagaagaa
     4681 atgaaaacat caaatcccca gatagttgcg atgacataga taacccaggt cagtacacca
     4741 cctaatgcgg cagggaaaat gtgcttaaat tgcaaatgaa ataagaagcc gaagccggca
     4801 gctccaataa aggctgcaaa aaattgtaat atcataacac acctccaaca aaccagatag
     4861 cgcacataaa tccacaggca agtgctcccg cccaaagaag cgattctaca agacgcatca
     4921 cgcctgaaat cgtgtcaccg attaaaatgt cacggataga gtttgtcata aggattccag
     4981 gaatcagcag cataatgtca ccgatcataa ctttgtctgc atgaattacc ggaaagaaat
     5041 gtgcagctac acaaatgccg gagccgacaa aaagggagca gaaaaactgc aggattaaag
     5101 tgttgctgca tatagagttt aaatgccttt gtaaaaagca aaggagaaag gcaaagataa
     5161 cggcaatggc tccgtctaaa attgttcctc caaaaaagac agaaaagcca cctgcaccaa
     5221 gcatacttcc caggtaaatg gtataaggag caggactgat ttttgaaatt ttatttatct
     5281 catatttcaa atctgcaagg gaaagaggag aagaacaaca gcgcctgctg agtgcatttg
     5341 cctgttctaa tttaaagaaa tctgtaccga caggggtagt gattcttcgg gtttgtgtat
     5401 attcttcgcc ttcttcaaag accatagtga caacgatact tgaagtgatt gcaaatacat
     5461 tcatctgggt ggcaccgtag gcatgcccca ttcggtttag cgtttcttcc acacggctga
     5521 cctcggcacc gttggtcagc ataatttccg caagatttag taattcatgt aaaatagcat
     5581 ttggtggatt cacatacttt ctcctttcac aaaaatatga tatagtatat cacaaattca
     5641 aaaaaaggag aaaaattttg agctatacct ggtatgaagt gttctggttt tttctgttat
     5701 attcctttgc aggatgggta ataggaactt ctggagcagc attaaaagaa aaaaagttta
     5761 ttgacgtagg atttttattt ggcccgtggt gtccatccta cggatttgga gccgttggtt
     5821 ttgcattatt tttaccagaa ttaaaagatc agcctgtatt tttatttgca ggaggagtga
     5881 ttctttcatt tattatcaca tcttttacag gagtgaccct ggaacgtatt ttccatcaga
     5941 aatggaaaga ttattccaga cggagattta actttggtgg ctatgttaac ttaccttata
     6001 cggtagtatg gggtgcggcg gcactgttgt gtatttggat tgtaaatcca tttttaagta
     6061 aaattattgg aatacttcca tatggattag gaaaagtcat tttgattatt gcctatgtaa
     6121 taatcattat tgattttatc ggaactgttt caggaattct gagtgtacat atccgcctgg
     6181 cacgattagg aaaactagtc cttgtagaag aagtagctga taacttacaa aaagctgcag
     6241 attacatggg aaatggtctg actggctggg tgttaaaaca tcttgaaaaa agccatgagg
     6301 gtctcgaaat caaggaaatc atccgaagta aactgcaaag acgagaaaaa gcacaggaaa
     6361 tgactggtgt atttgcagca ggatgtggat tttacaaact gttctggctg ttctttcttg
     6421 gctcttttct tggcgatatt acagaaacta tcttttgctg gtggaagatg ggcactatca
     6481 caagccgaag cagtgtagtt tatggcccat ttagccttgt ctggggaatt ggctgttttt
     6541 tcctcaccta tatgctctac caatatcgcg atagaagcga cagcttcatc tttatattcg
     6601 gaacagtcct cggcggtgct tacgaatata tatgtagtgt cgtattagag cttgtctttg
     6661 gaaccgtatt ctgggattac agcaaaattc catttaatct gggtggaaga atcaacttat
     6721 tgtactgttt cttctgggga atcgcagccg tagtctggat gaaaatatta tatccgaaac
     6781 tatcaggatt tattgagaag ataccaaaga aaatcggggt accattaacc tggttcttgg
     6841 ttgtattcat ggtattcgat atggcaatct caggtcttgc attaaaccgt tatcacgaaa
     6901 gacaccaaag tgcaaagaca cccgaaaccg ccataacgaa aatattagat acgagatttc
     6961 cagacacaag aatggaacgg atatacccaa gtgccaaaca tgtgaggtag ggaattctga
     7021 tttgcgtagc aaaggagacg tccacaggga atataatggt atgctccctt
//
