LOCUS       gnlProkkaCGNAIPGD_82    5502 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CGNAIPGD_82.
ACCESSION   gnlProkkaCGNAIPGD_82
VERSION     gnlProkkaCGNAIPGD_82
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 09:56:57
            Original ID  :: gnl|Prokka|CGNAIPGD_82
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5502
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5502
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[487:3688]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      488..3688
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5502
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5502
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             488..1798
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 190.9;
                     E-value: 4.6e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_1"
                     /sec_met_domain="CTP_transf_like (E-value: 7.1e-13,
                     bitscore: 37.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 8.3e-115, bitscore:
                     371.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 5.1e-62, bitscore:
                     198.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTVYLGMIGDIMHPGYINIINKATEYGDVIIGLFTDKAIANHR
                     RLPYLTWEQRKNVVESIRNVSRVVPQDDWSYVSNLLKYKPDYIIHGDDWQVGPDKYIRD
                     EVFKVMEKLGGEVIEIPYTQNISASGIKQEIDALGVTPQMRLSSLRRLIAAKPIVRILE
                     SHNGLTGLIAEHTKVEVNGQEREFDGMWASSLTDSTSKGKPDIEAVDLTTRLHDLNDTL
                     EVTTKPVIFDGDTGGKIEHFGFTVRTLERLGISCVIIEDKVGLKQNSLFGTDAVQTQDT
                     IEGFQAKIRAGKEAQITRDFMIVSRCESLIAGKTVDDALERCHAYVEAGTDGIMIHSKE
                     KSGEDIKEFCLRFREKNAHTPIIVVPTTYNQFTEEELAEWGINVVIYANHMLRSAYPAM
                     VKCAETILYTSRSLEASEQYCMPIKQILNLIPGTKKK"
     CDS             1833..2771
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 49.6;
                     E-value: 4.5e-15)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_2"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.5e-21, bitscore:
                     64.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.2e-17, bitscore:
                     51.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRPEFFVNILKEQGIDFYAGVPDSLLKNICAYITDHLPAEQNII
                     AANEGGAMGIAVGYHLATGKVPVVYMQNSGEGNIINPFASLTDKDVYNIPVLLVIGWRG
                     RPGVHDEPQHVKQGKVTIGLLNTMGINYAILPKDEEGAAKQIKIAVDFMKATNECYALV
                     IEKDTFDTYKLQSVEVNELAMSREETIQKVASNIEDNACIVSTTGMISRELFEARTAWS
                     QGHERDFLTVGAMGHASQIALGIALQKPERRVYCFDGDGASIMHMGNMAITASMKCKNY
                     VHIVFNNGAHDSVGGQLLLVSRLIFVQLLKL"
     CDS             2962..3279
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_3"
                     /sec_met_domain="Metallophos_2 (E-value: 8.4e-12, bitscore:
                     34.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVILFADIHANLSAFEAVLADIEKHYQPDAMISLGDFIDYGMRS
                     NEIIERIQTVNIPLLANLKGNHEKALIDGELSCFSSERARVMSKYTRNHLTQTSIDYIA
                     M"
     CDS             3279..3368
                     /locus_tag="ctg82_4"
                     /transl_table=11
                     /translation="MQEPMQELEIGGKKVLLLHGTIDDPIGEN"
     CDS             3380..3688
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_5"
                     /sec_met_domain="Metallophos_2 (E-value: 1.2e-12, bitscore:
                     37.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEDEKYQDFDYVLSGHIHQPFKIDVFFKVDNPIMRNQKRTTFINP
                     GSVGQPRNQNPYAQYVYINFKTGETHFNAVPYDIEAEQSLFPKEIDTFYSERLKFGI"
     CDS             3689..4684
                     /NRPS_PKS="Domain: NAD_binding_4 (83-198). E-value:
                     6.4e-10. Score: 30.4. Matches aSDomain:
                     nrpspksdomains_ctg82_6_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     143.1; E-value: 2.2e-43)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg82_6"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.8e-10,
                     bitscore: 27.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2e-09, bitscore:
                     25.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLYVISGVTGMTGSELSRQFVSQGHTVIGFDNFFASSIDTVKD
                     IIDNPSFIFYNYDINKVAQMDEVCDKAKELKIECDGRLVFINCAAVVHTKHFYEVESTF
                     QTNVLAMKDFLERAISMRADTYINCSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRT
                     SYACGKLMTEFFMKDAVGKGRIKGCSIRFANVYSRHERFAEHIIPHIIDNLLESHTVTL
                     LENSKVNKRTFLHNIDSCRAVMELIASEDALDGTVYNVATEEEIAIIDLVKTIAIKMGI
                     NDVDIRFEGYRKSDPERRLLNTDKIRTRTSWKPIITLDEGLTMCVNSIKK"
     aSDomain        3938..4282
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg82_6_NAD_binding_4.1"
                     /evalue="6.40E-10"
                     /label="ctg82_6_NAD_binding_4.1"
                     /locus_tag="ctg82_6"
                     /protein_end="198"
                     /protein_start="83"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VFINCAAVVHTKHFYEVESTFQTNVLAMKDFLERAISMRADTYIN
                     CSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRTSYACGKLMTEFFMKDAVGKGRIKG
                     CSIRFANVYSR"
     CDS             4681..5499
                     /locus_tag="ctg82_7"
                     /transl_table=11
                     /translation="MKYLIITVAGTATRFNRDTKEETLKCLYYTDEPQYALLAQLLKNC
                     GEYDKYILVGGYLYEKLGRFVKNELSGYGKKIELVYNEHFKDYGSGYSLYKGIEAIKEA
                     GDVTFVEGDLFFMATDFRQVYNSPKSVITINREPIYSNKAVALYINVDGKPRYLYDTNH
                     QTLTVSEPFKAIFNSGQMWKFSSSERLLKTVDSLTKEQLQGTNLEIIQAYFSDMNSEEY
                     DVVTFTDWFNLEIIQAYFSDMNSEEYDVVTFTDWFNCNTVADYDIVRKLM"
ORIGIN
        1 ttatagaatt atgtatagca gtgaaagatc tgtaaagttt tttactttca tttagggcac
       61 tgaaaagtgc ctttcaaagg gggatgccct aaattgagtg cttactgaga ttctctctaa
      121 tacagacaag ttcttgtttg cggacaaaag taaggcttgt cttttttgtt gaaacctata
      181 actatgcttc tcataatggg gagttcataa tggggagtgt tcgttgcaat tgacagaaag
      241 ccacagaact tccgcatcat cattgttact ccaactcaat ggtatatcca ttcttctttt
      301 agcacaatgg tagagtccta cagttccttt gctctccgca aatggagaaa cttattgagt
      361 ggttcagaag tgtagtcgaa cttatggtaa atctatctat gtgtagtata cttattctca
      421 agcaggagaa attgaggtga ttgactatag atgctcttta taataatttg aaaaatgtta
      481 aataataatg gcaaagacag tttatcttgg tatgataggc gatatcatgc atccgggtta
      541 cattaacatc attaacaaag ctactgagta tggcgatgtg ataatcggtt tgttcactga
      601 taaggcgatt gctaaccacc gtagacttcc ttatctcact tgggagcaaa gaaagaacgt
      661 tgttgagagc atcaggaacg tatcaagggt ggtccctcag gatgattgga gctatgtctc
      721 taatctcctt aagtacaagc ctgattacat cattcatggt gatgattggc aggttggtcc
      781 tgacaagtat atccgtgatg aggtattcaa agtaatggaa aagttgggtg gtgaggttat
      841 cgagattcct tacacccaaa acatctctgc ttctggtatc aaacaagaga ttgatgctct
      901 tggtgtaact cctcagatgc gtctttcttc tctccgtcgc cttattgcag ctaaaccgat
      961 tgtacgtatt cttgaaagtc acaatggtct tactggcctc atagcagaac atactaaggt
     1021 agaggtgaat ggtcaggagc gtgaattcga tggcatgtgg gcttcctccc ttaccgactc
     1081 tacctctaag ggtaagcctg atattgaggc tgtcgatttg actacccgtc tccatgacct
     1141 caatgatacc cttgaagtaa ccacaaaacc tgttatcttc gatggcgata ctggtggcaa
     1201 gattgaacac ttcggtttta ccgttcgcac ccttgagcgc ttgggaatct cttgcgttat
     1261 aattgaagat aaggtaggct tgaaacagaa ttcacttttt ggtactgatg ctgtacagac
     1321 tcaggataca atcgaaggtt ttcaggctaa aatccgtgcg ggtaaggagg cgcagattac
     1381 tcgtgacttc atgattgtca gccgttgtga gtctctcatt gctggtaaga ctgtagatga
     1441 tgctttggag cgttgtcatg cttacgtaga ggcaggtact gacggtataa tgatccactc
     1501 caaagagaag tctggagagg acatcaagga gttttgcttg cgctttcgcg agaagaatgc
     1561 tcatactcct atcatcgttg tgcctactac ttataaccag ttcacagaag aggaacttgc
     1621 tgaatggggt attaatgtag tgatttatgc taatcacatg cttcgttctg cttatcctgc
     1681 aatggttaaa tgtgcagaaa ccatcctcta tactagccgt tcgcttgaag catctgagca
     1741 gtactgtatg cctatcaaac agattcttaa tcttattcca ggcacaaaga agaagtaaac
     1801 attaatttct taccccttaa actattttac aaatggtaag acccgaattt tttgtaaata
     1861 tactcaagga acaaggtata gacttttatg ctggtgttcc tgattcgttg ctcaagaaca
     1921 tttgtgcata tattaccgac caccttcctg ctgagcagaa tattattgct gctaacgaag
     1981 gaggggccat gggtattgct gtaggttatc atttggcaac aggtaaggtg cctgtagttt
     2041 atatgcagaa ctctggtgaa ggcaatatca ttaatccgtt tgcctctctt accgacaagg
     2101 acgtttataa cattcctgtg ctcctcgtta tcggttggag agggcgccct ggagttcacg
     2161 atgaacctca gcatgtgaag caaggtaagg tgacaatcgg tttgcttaat accatgggta
     2221 tcaactatgc tattctgcca aaggatgaag agggagctgc aaagcagata aaaatagctg
     2281 tcgacttcat gaaggcaacc aacgagtgct atgcccttgt gatagagaag gatactttcg
     2341 atacctataa gcttcagagc gttgaggtaa acgaactggc aatgtctcgc gaagagacga
     2401 ttcagaaggt ggcttcaaac attgaagata atgcttgtat cgtatcaact actggtatga
     2461 tttctcgcga gctctttgag gcacgtactg cttggagcca aggtcatgaa cgtgatttcc
     2521 ttactgtagg ggcgatgggc catgcttccc agatagctct cggcatagct ctccaaaagc
     2581 ctgagcgtcg agtatattgt tttgatggtg atggagccag catcatgcac atgggtaata
     2641 tggctattac agccagcatg aaatgcaaga attatgtgca cattgtgttc aacaatggcg
     2701 cacacgattc tgttggtggt cagctactgt tggtctcaag attgattttt gtgcaattgc
     2761 tcaagctgta ggctacaagg ctgcttatag tgtagaaact atggaggcac ttgaagcaaa
     2821 acttgcagaa ttcaagaacg cagaaggtcc tgttttcctt cagatttgcg taaaaaaggg
     2881 aaaccgcaag gacttggccg tccaactaca actcctattc agaacaaaga ggcttttatg
     2941 gaatttctta acaagtaaag tatgaaagta attctgtttg ctgatataca cgccaatctt
     3001 tctgcttttg aggcggttct agctgatatt gagaaacact atcagccaga tgccatgatt
     3061 agtctaggtg actttatcga ctatggtatg cgttcaaatg aaattattga acgtatacaa
     3121 actgttaata ttccattact tgccaatttg aagggcaatc acgagaaggc tttgattgat
     3181 ggggaactct catgtttctc ttcagaaaga gctcgtgtga tgtccaaata cacaagaaac
     3241 catctcactc agacatctat tgattatata gcaatgtgat gcaagagcca atgcaagagt
     3301 tggaaattgg tggtaagaaa gtacttcttt tacatggaac tattgatgat cctattgggg
     3361 aaaactgact ccagcaaaag tggaagatga aaagtatcag gattttgact acgtattgag
     3421 cggtcacatt catcaacctt ttaagataga tgtattcttc aaagttgaca atccaatcat
     3481 gcgcaatcag aaacgtacga cttttatcaa cccaggttca gttggacaac ctcgcaatca
     3541 gaatccatat gcacaatatg tgtatatcaa ttttaaaacg ggggaaaccc atttcaatgc
     3601 agttccttac gatattgaag ctgaacaatc tcttttcccc aaagagatag atacttttta
     3661 cagcgaacga ttaaaatttg gaatataaat gaagaactta tatgtaataa gtggtgttac
     3721 tggcatgacg ggtagtgaac tgtctcgtca gtttgtttct cagggtcata cggtaatcgg
     3781 tttcgacaac ttctttgcaa gttctattga taccgttaaa gacattattg acaatccctc
     3841 gttcatcttc tataattacg acattaacaa agtcgctcag atggatgaag tatgcgataa
     3901 ggcgaaagaa ctcaaaattg agtgcgatgg tcgtctggtg tttatcaatt gtgctgctgt
     3961 tgtccataca aagcatttct atgaggtaga gagtactttt cagactaacg tccttgctat
     4021 gaaggatttc ttggagcgtg ccatttctat gagggctgat acttacatta attgttcaac
     4081 ttcggaggtt tactcaatgc agtcttggaa tgagaatggc ggtgtgaaag aatctgatta
     4141 tttggcaatg tcaacagcgg aacatagcca acgtacaagt tatgcttgcg gcaagttgat
     4201 gactgaattc tttatgaaag atgctgttgg caagggacga attaaaggtt gttctattcg
     4261 ctttgctaat gtatatagca ggcacgagcg ttttgcagag cacattatcc ctcatatcat
     4321 tgataatctg cttgaaagcc ataccgtaac ccttcttgaa aactcgaagg tgaacaaacg
     4381 taccttcctt cataatattg atagctgtcg tgccgttatg gagctcatcg cttctgagga
     4441 tgctcttgat ggtactgttt ataatgtggc tactgaagag gaaattgcca ttattgatct
     4501 agtcaagacg attgcaataa aaatgggcat caacgatgtg gatatccgtt tcgaaggtta
     4561 ccgaaagtct gatcctgagc gtcgattgct caatacggac aagattcgta cccgtactag
     4621 ctggaaacct atcatcactc tcgatgaagg cctcacgatg tgtgtaaata gtatcaagaa
     4681 atgaaatatt tgattataac tgttgctgga acggctacgc gttttaatcg cgacactaag
     4741 gaagaaaccc tcaaatgctt gtattatacc gatgaacctc aatacgctct tcttgctcaa
     4801 ttgctgaaga attgtggtga gtacgataaa tatatccttg tggggggcta cctttatgag
     4861 aagttgggaa gatttgtaaa gaacgaactc tctggttatg gcaaaaaaat agaacttgtt
     4921 tacaacgagc atttcaaaga ctatggctcc ggctacagtt tatacaaagg tatagaggca
     4981 ataaaggagg caggtgatgt gactttcgta gaaggcgact tgttctttat ggctacagat
     5041 ttcaggcaag tgtataattc gccaaagagt gtgattacca ttaaccgaga accaatctac
     5101 tcgaataagg cagttgcgtt atacattaac gttgatggca aaccaaggta tttatatgat
     5161 acgaatcatc agacattgac tgtatcagag ccattcaagg caatcttcaa cagtggtcag
     5221 atgtggaaat tttcttcatc tgagaggctt ttgaagactg ttgattcgct tacaaaagaa
     5281 caacttcaag gtactaatct tgaaataatt caggcttact ttagtgatat gaatagtgaa
     5341 gagtatgatg tagtgacttt cactgactgg ttcaatcttg aaataattca ggcttacttt
     5401 agtgatatga atagtgaaga gtatgatgta gtgactttca ctgactggtt caactgcaat
     5461 actgttgcag attatgatat tgtaagaaaa ttgatgtaaa aa
//
