LOCUS       c00099_gnlPro..        14924 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IMNLIBKC_111.
ACCESSION   c00099_gnlPro..
VERSION     c00099_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 17:34:59
            Original ID  :: gnl|Prokka|IMNLIBKC_111
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14924
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14924
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[64:4924]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      65..4924
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..14924
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14924
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             65..1297
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_1"
                     /sec_met_domain="HBT (E-value: 2.5e-17, bitscore: 56.5,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNG
                     FALFTYHDDTIITDSPENEHIQNEARLNNLQIIKEEEFSAAVDFAPCKCMLVSDDEEAL
                     VGLEDHWKRRLNGALDVFRSEPYFLEVVPCAIDKANSLGALLEVLDMKREEVIAVGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDGVAVAVEKAIIAEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALAETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             1294..2409
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg99_2"
                     /sec_met_domain="Chorismate_bind (E-value: 6.8e-57,
                     bitscore: 186.3, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGAVRL
                     IYDLKELNGQRGFVIAPFQVSESCPVVLIQPDQWGQPLPMDDDTEEDREIALRLQGQES
                     FLTSSTEEYAACFHTFINALRDRTFDKLVLSRHLTVDKVADFSPLSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLDIHSTENGPYPAYAGALSHLKTDFRFSLKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYQFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIEDKQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIAVPPLKS"
     CDS             2429..4096
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.4e-06, bitscore:
                     21.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVIISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINVPISEPLFQFTVDSLPEVRVITRYQGLNVYDRDYNDLVDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVGQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKIIPEPEFAYSEMSAVGTLLKALPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTNGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNVNVRSNLRV
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             4100..4924
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_4"
                     /sec_met_domain="ECH_1 (E-value: 1.5e-81, bitscore: 266.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             5097..6122
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg99_5"
                     /sec_met_domain="MR_MLE_C (E-value: 2.7e-27, bitscore:
                     89.1, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILVKVCRKVEREQGRLDMDGLCDYPSILFGLETAIRHFF
                     AGNWALCDTAFSRGEVGIPINGLIWMGDFQNMLSQIEKKMEAGFRCIKLKIGAINFEEE
                     LTLLRHIRTHFSSKEIELRVDANGAFSPVDAMEKLKRLSEFDLHSIEQPICAGQWEEMA
                     RLAAESPLAIALDEELIGCNTLERKQELLSTIRPQYIVIKPSLHGGICGGDEWITEAEK
                     QQIGWWITSALESNIGLNAIAQWCATFSNPLPQGLGTGALFTDNVEVPLEIRKDCLWFC
                     K"
     CDS             6144..7280
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 57.1; E-value: 4e-18)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg99_6"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-17, bitscore:
                     57.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFDRKQQRLLLEGKEYTFEDISRLIAGGAEAHSPASWDLYLFLN
                     EWFNDSPVIIVHTSGSTGTPKELIVRKDQMMQSARLTCEFLDLKKGETALLCMNLRYIG
                     AMMVVVRSLVAGLNLIVRPASGHPLADIKEPLRFVAMVPLQVYNTLQVPEEKERLKQTD
                     ILIIGGGAIDEALEAEIKHLPTAVYSTYGMTETLSHIALRRLNGTLASEHYYPFSSVKL
                     SLSVENTLIIDAPLVCDEILQTNDIARIYPDGSFMILGRKDNVINSGGIKIQAEEMEKL
                     LRSFIPVPFVITSVPDQRLGQAVTLLLEGQPELLEGQPDTKEIGNKLQEILEPYYRPKY
                     IWTTDCIPQTENGKVNRAKCRILAKQMLTTFYTHTLTP"
     CDS             complement(7591..8067)
                     /locus_tag="ctg99_7"
                     /transl_table=11
                     /translation="MKTLEFIKLNESGANNVVASLQQLLADFQVYYTNLRGFHWNIKGH
                     DFFVLHSQFEKMYDDTAEKVDEIAERILMLGGTPANKFSDYLKVANISEVDKVSNGEQA
                     LNNILQSISYLIGEERKILSIASQAGDEVTVSMMSDYLKEQEKLVWMLTAYNSK"
     CDS             8206..9132
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     61.1; E-value: 2.4e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg99_8"
                     /sec_met_domain="LysR_substrate (E-value: 2.9e-43,
                     bitscore: 141.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 6.4e-19, bitscore: 61.1,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIQQLEYILAVDQFRHFARAAEYCRVTQPTLSAMIQKLEDELGV
                     KLFDRTVQPVCPTPIGQKVIDQARVILAQAAQVKEIISEDKQSLSGVFRLGVLPTIAPY
                     LLPRFFPQLMEKYPELDIRVTEMKTQDIQQALHAGDLDAGIIASKLEDTFLTEETLFYE
                     QFYAYVSRKEPSFKHDVIRTSDITGEHLWLLDEGHCFRDQLVRFCQMEAVKVSQMAYRL
                     GSMETFMRMVESGKGITFIPELAVAQLTEEQRQLVRPFAIPRPTRQVVLATNRDFIRHS
                     LLCVLKEEIKAAVPKEMLTLQSIQCLL"
     CDS             complement(9545..10267)
                     /locus_tag="ctg99_9"
                     /transl_table=11
                     /translation="MKKFKFIAFIFAIMTTMPILQSCLDDDDNPSDLLVVSTINMISQD
                     SKEFYFTLDDGKKMYPSNAQGWSNKDWVEGQRAFVIFNELEEPVNGYDLNIQVRGINPI
                     LTKDIITMGEDDNDEEKVGDDKINTTYMWINKDNKYLTIEFQYYGTHSEDKKHFLNLVI
                     NDKEESAPTADEDSAEDEYINLEFRHNSEGDYPQSLGEGYVSFKLDKIKDRMEGKKGLR
                     IRVNTLYGGPKTYEVKFP"
     CDS             10444..10992
                     /locus_tag="ctg99_10"
                     /transl_table=11
                     /translation="MEELELSEQCRLGNNQARKELYEQYAGRMLGICLRYTGDRDTAQD
                     LLHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQYLRKNDVINQSAPLEELPEEY
                     EEPDASDVEAIPQKVLMQFIEELPAGYRTVFNLYTFEDKSHKEIAQELGINEKSSASQL
                     FRAKSVLAKRVKEWITHNG"
     CDS             10999..12297
                     /locus_tag="ctg99_11"
                     /transl_table=11
                     /translation="MEEKELWMNKLKEKLADYSEPVPASGWEQLEKELMPPVEKKIYPY
                     RKWMMAAAAVILLAVVSSVSLYFLGTPAADEIRHIKTPALASTPDALPGVQQPDVQGTS
                     VEPVLRPVAREDRLAKIDKNLTEQKTNAGQSAIENNNEPVTGNENNPVIEEDQMLKGET
                     EQTKNEARQVDSENVGQATQSQDTERPNNRPRRPSGRDKLHIPAEKRASQKGTWSMGLS
                     VGNSGGASTEVGAGSHAYMSRVSMLSVSNGLMEIPNDQTLVFEDGVPYLRQAKQVVDIK
                     HHQPISFGLSVRKGLAKGFSLETGLTYTLLSSDAKLAGEDQQIEQKLHYVGIPLRANWN
                     FFDKKLVTLYVSGGGMVEKCVYGKLGSEKETVKPLQFSVSGAVGAQVNATKRLGIYVEP
                     GVAYYFDDGSDIQTIRKENPFNFNIQAGIRLTY"
     CDS             12510..13106
                     /locus_tag="ctg99_12"
                     /transl_table=11
                     /translation="MVNFTDEKHLLIDLKDGSFQAFERLYNMYSGKLYNFIMRLSSGNQ
                     YMAEEVVQSTFIRIWEVREKVDTNASFISFLCTIAKNLLMNMYQRQTVEYVYNEYLLKS
                     SVDRDSQTEENIDLHFLNDYIDSLAEELPAQRKKIFILSKRQNYTNKEIAEMMGISEST
                     VATQLSLAVKFMREQLMKHYDKVIALLIAFFVNEM"
     CDS             13174..14154
                     /locus_tag="ctg99_13"
                     /transl_table=11
                     /translation="MDKIYYKELIEKYFDGNITDAEIKELSDWIKNDRHLQNWWEEEFS
                     KSDAGINPVLRDKLFARIKEQTQGKEETQEKEKPRTIRMNPWKWAAAIVLPICIAFFTY
                     YLIDSSQTVGAPFIVKADKGDKATIELPDGTNVVLNSASQLSYLNNFGENVRRVQLNGE
                     AYFKVAHDEKHAFIVQVGDLEVKVLGTSFNVSAYEDAKDVTVVLLEGKVGVYAQKMSHI
                     MKPGDKIEYNKATHKITATQVHPSDYIEWTKGNIYFEKESLENIMKTLSRIYDVEIRFD
                     SNKLPNEYFTGTIPVGGIQNALNILMLTSPFYYEMDGSVIVLKEK"
ORIGIN
        1 tgccggtgta gaaaaactcg atttttctat ataattttgt aatctaaatc aaaatcgtgt
       61 aatcatgaag tataaattat tggttcttga tgtagacgga acactcctca atgatgcgaa
      121 agaaattagt aagcgtacat tggctgccct gttgaaggtt cagcaaatgg gagtacgtat
      181 tgtgctggca tccggcagac cgacttatgg cctgatgccg ttggccaaga tgcttgaact
      241 tggaaactat ggaggtttta tcctttccta taatggctgt cagattatca atgcacaaaa
      301 cggggaaatt ctgtttgaac gtcggattaa tcccgaaatg ttgccgtatc tggaaaagaa
      361 agcccgtaaa aacggttttg ccttattcac ttatcatgat gatacgatta taacggattc
      421 tcctgaaaat gaacatatcc aaaatgaagc ccggttgaat aacctgcaaa taattaaaga
      481 agaagaattt tctgctgccg ttgattttgc tccttgtaag tgtatgctgg tgagcgatga
      541 cgaagaagca ctggttggtt tggaagatca ctggaagaga cgactgaacg gagcgttgga
      601 cgtattccgt tcggagccat attttctgga agtagttccc tgcgccatcg acaaagccaa
      661 ctctttgggc gctttgctgg aggtgctgga tatgaagcgt gaggaagtga ttgctgtggg
      721 cgacggtgtg tgtgacgtaa caatgattca attggccggg ctgggcgtag ctatgggaca
      781 ttcacaagac tcggtgaaag cctgtgccga ctatgtgacc gcttcgaatg aggaagacgg
      841 agtggccgtt gcagtggaga aggctattat agctgaagtg cgtgcagccg aaattcccct
      901 tgaccaactg aatgcgcagg cacgccacgc attaatggga aacttgggaa ttcaatatac
      961 atacgccgac gaagaccgtg tggaagcaac gatgccggta gaccatcgta cccgtcagcc
     1021 tttcggcatt ttgcatgggg gagctactct tgcactggca gaaacagttg ccggactcgg
     1081 ttcgatgatt ctttgccagc cggacgagat tgtagtagga atgcaggtca gcggaaacca
     1141 tatatcttct gcacatgaag gagatacagt gcgtgcggta ggaacagttg tacataaggg
     1201 acgttcctcc cacgtatgga atgtagacgt gtttacttca acaaacaaac tggtgtcttc
     1261 catacgggta gtcaacagtg tgatgaaaaa gagatgaccg acgaagaaat aagtaacttg
     1321 acaactattg atgcatttat tcagcggaag cagccgtttg ctgtttaccg tattcccgga
     1381 gagaaggttc cccgtctgtt gacgcaggcg gaaggagcgg tccgtttgat atacgatttg
     1441 aaagaactga acggacagag aggatttgta attgctccgt ttcaggtgag tgagtcatgt
     1501 ccggttgtgt tgatccaacc cgatcagtgg gggcagcctt taccgatgga tgatgatacg
     1561 gaagaagacc gggagatagc cttgcgcctg caaggacaag aatcatttct gacttcttct
     1621 acggaagaat atgcggcatg ctttcatacc tttataaatg cgttacgtga caggacgttt
     1681 gataaactag tgctttcccg tcatcttaca gttgataaag ttgcggattt ttctcctttg
     1741 tccatctttc gggcggcttg taaacgttat attcattcgt atatttactt atgttatact
     1801 ccgcagacgg gcatctggct gggaagtact cccgaaatca ttttatcagg tgagaaagac
     1861 gaatggcata cggttgcact agccggaaca cagcctttgc aagatggcag attgccgcaa
     1921 gtatgggatg aaaagaaccg gaaagaacaa gattatgttg cttcttatat ccgtcggcag
     1981 cttctttcac tggatattca ttctacggaa aacgggccat atcctgctta tgccggtgct
     2041 ttgtcacatt taaaaactga tttccgtttt tctttgaaag ataataaagg tttgggagac
     2101 cttctgaaag ttttgcatcc gacacctgcc gtatgcggct tgccgaagga agaagcctat
     2161 cagtttattt tgcagaatga aggttacgac cgccgctatt actccggctt tatcggatgg
     2221 ctcgatccgg aaggacgaac agacatctat gtaaacttac gctgtatgca tatcgaagat
     2281 aaacaactta ctctttatgc cggcggtgga ttattggcct cttcggaatt gaatgacgaa
     2341 tggctggaaa cggagaagaa gttgcaaact atcaaacggc tgattgcagt gcctccttta
     2401 aaatcataaa ctactaataa ttatcatcat gtatacagac aagaagaaca tactccagtt
     2461 ggttgcgttg ctcgaggcgc atgggattac taaggttgtg ttgtgtccgg gtagtcggaa
     2521 tacgcccata gtacatactt tgtctaacca tccgaatttt acctgctatc cggtgacgga
     2581 tgaacggagt gccggctatt ttgccatcgg tcttgcgttg aatggtggta agcccgcagc
     2641 tgtctgttgc acttccggta ctgcattatt aaaccttcat ccggctgtgg cagaagcttt
     2701 ttatcaaaat gttcctctgg tcattatttc tgcggatcgt cccgctgcct ggatcgggca
     2761 gatggatgga cagacagtac cacaaccagg cgtatttcag acattagtta agaagtcggt
     2821 aaaccttccg gagattcata cggaagaaga tgaatggtat tgcaatcgtc tggtgaatga
     2881 agctttgctg gaaacgaacc atcatggaaa aggaccggta catatcaatg tccctatttc
     2941 tgaaccgttg ttccagttta cggttgattc gcttcctgaa gtgcgtgtca ttacccgtta
     3001 tcaggggttg aacgtctacg atcgtgatta taacgatttg gtcgatcgga tgaataaata
     3061 ccagaagcga atgattatta tcggtcagat gaatcttatc tacctgtttg aaaaaagata
     3121 tatcaagtta ttatataaac attttgcctg gttgacggaa catatcggca accagacagt
     3181 tcccggtatt ccggtaaaga actttgatgc ggcgctttat gccatgcccg aagagaaggt
     3241 cgggcagatg gttccggagt tgctgattac ttatggagga catgtcgttt ccaaacggct
     3301 gaagaagttt ctccgtcagc atccgcctaa agaacattgg cacgtatcgc cggatggtga
     3361 ggtggtcgat ttgtacggct cattaactac ggtgatcgaa atggacccgt tcgagtttct
     3421 ggagaagata gccagtttgt tggacaatcg gactcctgaa tatccccgtg tgtgggaaaa
     3481 ctattgcaag atcattcccg aaccggagtt tgcttattcg gaaatgtctg ccgtcggtac
     3541 gttattgaaa gccttgccgg agtcatgtgc gttgcatctg gctaacagtt cggtggtgcg
     3601 ctatgcacaa ttatactcta ttccttctac gattgaagtt tgttgcaacc gggggacaaa
     3661 cggcattgaa ggctcactgt ctacggctgt cggttatgcg gcagcttccg acaaactgaa
     3721 ctttatagct atcggagatt tgagcttctt ttatgatatg aatgcgttgt ggaacgtcaa
     3781 tgttcgctcc aatctgcgtg ttcttctgtt aaataatgga ggaggagaga ttttccatac
     3841 cttgccggga ttggatatgt cgggtacctc acataaattt attgcggcag tccataaaac
     3901 gtccgccaaa ggttgggcag aagagcgtgg gtttctctat ctgcaagcgc aaaatgacga
     3961 agaactggct gaagcgatga aaactttcac tcaaccggaa gctatggagc agcctgtgct
     4021 cttggaggtg tttaccaaca aaaacaagga cgcgcgcatg ttgaagaatt attatcatca
     4081 attaaaacaa aaatagatta tgtcaacaca aagagagtgg acaaccatca gagaatacga
     4141 cgatattctt tttgattatt ataatggaat tgcccgtatc acgatcaacc gtgaacgcta
     4201 tcggaatgcg tttactccga caactactgc tgaaatgagt gacgcattgc gcatctgccg
     4261 tgaagaagcg gatattgatg tgattgtgat taccggagcc ggtgataagg cgttttgttc
     4321 gggtggcgac cagaatgtga aaggacgtgg cggatatatc ggaaaagacg gtgtgcctcg
     4381 tcttagcgta ttggatgtgc aaaagcagat tcgcagcatc ccgaaaccgg tgattgctgc
     4441 cgtgaacggt tttgctattg gtggcggaca tgtgcttcat gtggtatgtg atttgtccat
     4501 tgcgtcggaa aatgctattt tcggtcagac gggtcctcgt gtaggtagct ttgatgccgg
     4561 attcggtgcg tcttatctgg cacgtgtggt aggtcagaag aaagcacgcg aaatctggtt
     4621 cctctgtcgg aagtataatg cacaagaagc attggacatg ggattagtga ataaagtagt
     4681 tcctttggag cagttggagg acgaatatgt gcagtgggca gaagaaatga tgttgcttag
     4741 tccgttggct ttgcgcatga ttaaagcagg attgaatgcc gaactggatg gtcaggcagg
     4801 tattcaggaa ttggcaggtg acgcaacgtt gctctattac ctgactgatg aagcacagga
     4861 aggaaagaat gcgtttctgg agaaacgtaa accgaacttc aaacaatatc cgaaattccc
     4921 ttaaaatcgg ctttgccaca gagtaccaca gagtttgctt ttcctgcgtt taagcccgat
     4981 tttctgcaaa gcaagactgt ggaggagaga atggtaggag aactgcaaga gatataactt
     5041 attaaaactt cgtgaggctc tgtgttattc tgtggtgaat tgaaaaatta ttcacgatga
     5101 attgtaaaat agaaattatt ccccgattgc tccatttcaa gcaacctgcc gggacttcca
     5161 gaggaacata tactacgcgt aaagtttggt atcttcattt tacctctcct gacttccctg
     5221 gcagagtggg aataggggag tgtgcacctt tgcccgcttt aagttgtgac gatcttcctg
     5281 attacgaaga tatattagtg aaagtctgcc ggaaagtaga gagggaacaa ggcagattgg
     5341 atatggatgg cttgtgtgac tatccttcaa ttttatttgg tctggaaacg gccatccggc
     5401 atttctttgc aggaaactgg gctttatgtg atacggcttt ctcacgtgga gaagttggta
     5461 ttccgattaa cggccttatt tggatgggag attttcagaa catgttatcc cagattgaga
     5521 aaaagatgga agccggattt cgttgtatca agttaaagat aggtgccatc aatttcgaag
     5581 aagaactgac attgttgcgc catattcgta cgcatttctc ttctaaagaa atagagttgc
     5641 gtgtagatgc aaatggtgct ttctcacccg tggacgcaat ggaaaaactg aaacgtctgt
     5701 ccgagtttga tttacattct atcgaacagc caatctgtgc cggacaatgg gaggaaatgg
     5761 ctcggcttgc tgccgaatct ccattagcga ttgctttgga tgaagaactg ataggatgca
     5821 atacgctgga aaggaaacag gagctgcttt caacaattcg tccgcaatat atcgttatta
     5881 aaccttctct tcatggagga atatgcggag gagacgaatg gattaccgaa gctgaaaaac
     5941 aacagatcgg ctggtggata acttccgctc tggaatcaaa cattgggcta aacgccattg
     6001 cccagtggtg tgcaacattc agcaatccgt tgccacaggg actgggaaca ggggcgctct
     6061 ttacagacaa cgtggaagtg cctcttgaaa taagaaaaga ttgtttatgg ttttgtaaat
     6121 gatagtattt aaaggatatt ccgatgatat tcgatcgaaa acaacaacgc ttgttactgg
     6181 aaggaaaaga atatactttt gaggatataa gccgattaat agcaggagga gcggaggctc
     6241 attctcctgc tagttgggat ttatatcttt tcctgaacga atggtttaac gattctcctg
     6301 tcatcattgt tcatacatca ggctctacgg gtacacctaa agagttgatt gtacgtaaag
     6361 accagatgat gcaaagtgcg cgtctgactt gtgagtttct ggatttgaag aaaggagaga
     6421 cggcattact ttgtatgaat cttcgttata ttggtgcaat gatggtggtc gtgcgttcgt
     6481 tggttgcagg cttgaatttg attgtacgtc ctgcttccgg tcatccttta gcggatataa
     6541 aagagccttt gagatttgtg gcaatggttc ccctgcaagt ttataatacg ttgcaagttc
     6601 ccgaagagaa agaacgattg aaacagacgg atattttgat tataggtggt ggagctattg
     6661 atgaggcttt agaggcggag atcaaacatc ttccgactgc cgtctattct acctatggca
     6721 tgactgaaac tttatctcat attgctcttc gtcggctgaa cggaacattg gcttcggaac
     6781 attattatcc gttttcttca gtaaaactgt ctttatctgt agagaatacg ttaattattg
     6841 atgccccttt ggtatgtgat gaaatattgc aaaccaatga cattgcacgc atctatcccg
     6901 atggaagttt tatgattttg ggtcggaaag acaatgtgat aaatagtgga ggaattaaga
     6961 ttcaggcgga ggaaatggag aaattgcttc gttcttttat tcctgtgcca tttgttatta
     7021 cttcagttcc ggaccagcgt ttagggcagg ctgtaacctt actattggaa ggtcagccag
     7081 aattattgga aggtcagccg gacactaaag aaatcggaaa caaactacag gaaatattgg
     7141 aaccttatta tcgtcccaag tacatatgga cgacagattg tattccgcaa acagaaaacg
     7201 gcaaggttaa tcgtgcgaag tgtcgtattt tagccaaaca aatgttgacg acgttttaca
     7261 ctcacactct cacaccttga ttgaaatcct ttattcatca acgtttcagg ggcctctaat
     7321 tgtcttaccc tagttttagt tgagacttat tagagtcaac aaaattcagg gtaagacatt
     7381 ttatttcagt agaataagtt tcatcttttg aaagttgttg cttactttta taaatcttga
     7441 taaataaaga acaaaactaa tttttttaat aatatcagcg aatgaacgta tttagatagt
     7501 actagtacac tggaaataat aaagagagaa tattcctttt acggaaatat cctctctttc
     7561 tcacttaaaa acctaaacct tttatcttaa ttatttgcta ttataagccg tcaacatcca
     7621 aactagtttt tcctgttctt tcagataatc gctcatcatc gaaacggtta cttcatctcc
     7681 ggcttgagaa gcgatagaca agattttacg ttcttcacca atcagataac tgatactctg
     7741 aaggatattg ttcagtgcct gttcgccgtt gctcacctta tctacttcac tgatatttgc
     7801 tactttcaaa taatcgctga atttattggc aggagtaccg cccaacatca agatacgttc
     7861 tgcaatttcg tctacctttt cggctgtgtc atcatacatc ttttcgaatt ggctgtggag
     7921 aacaaagaaa tcatgtcctt tgatattcca gtggaaaccg cggagattgg tgtaatacac
     7981 ttggaagtct gctaatagtt gttgcaaaga tgctactacg ttgtttgctc ccgattcatt
     8041 cagtttgata aattctaaag ttttcatatt ctttttattt ttaatgattt atacttgttt
     8101 tctttttttt ctgtactgca aagataaggc ggaaaatgct tgttgtcaaa ctgaaaattt
     8161 ctatctttgc atagatgaaa tctataactt aatatgattt gatagatgac gatacaacaa
     8221 ttagaatata tactagccgt agaccagttc aggcatttcg cacgggcagc cgaatattgc
     8281 cgtgtgacgc aacctacttt gagtgccatg attcagaagc tggaagatga attgggagta
     8341 aagttattcg ataggacagt gcaacccgtc tgcccgacac ccatcgggca gaaagtgata
     8401 gaccaggctc gtgtgattct ggcgcaggca gctcaagtaa aagaaatcat cagtgaagat
     8461 aagcaatcat tatcaggtgt attccgtttg ggagtattac ccactatcgc tccttatttg
     8521 ctaccccgct ttttccctca actgatggag aaatatcccg aactggatat tcgtgtcacg
     8581 gaaatgaaaa cccaggatat tcagcaggct ctgcatgccg gcgatttgga tgcagggatt
     8641 attgccagta aattagaaga cacattcttg acggaagaaa ctctcttcta tgagcaattt
     8701 tatgcttatg tatcacggaa agaaccctcg ttcaaacatg acgttatccg cacttccgat
     8761 ataaccggcg aacatctttg gcttttggac gaaggacatt gctttcgtga ccaacttgtc
     8821 cgtttctgtc agatggaagc ggtgaaggtt agtcagatgg cttatcgttt gggaagcatg
     8881 gaaaccttta tgagaatggt agaaagcgga aaaggaatca cttttattcc cgaactggca
     8941 gtggctcaat tgacggaaga acagcgacaa ttagtacgtc cgtttgctat cccccgaccg
     9001 acgcggcaag tggtgttggc gacaaataga gattttattc gtcatagcct gctatgtgtt
     9061 ctgaaagagg aaataaaagc tgcagtcccc aaagaaatgc ttactttgca gtccatacaa
     9121 tgtttgctat aataaatagg agattgagag gattccggag aaattcatag gagtgaagtc
     9181 atagctcgat ccttgcttga taaatgtgtt tacgatgtgc tccatgtggt agaggcaaat
     9241 atgatgtaaa aaagtccttt gttcatttta ttggcttgtc tttgctttca tttttgtatt
     9301 agtggtgctc ggctctaccg attcgaagag gggagcgggc aatctggcag gacttgccat
     9361 cggtttgact cttgtattgg tacatattgt atgtattccc atcacaggga cttcccttta
     9421 taagaataac ttcttatata aagataaaag ggcggcagtt tcacaactac cgccctcttt
     9481 tgcgtaatta atatattata ttaaaaaaga gtgtgttttg cacgtttaca ttagatttat
     9541 aatcttaggg aaactttact tcataagttt tcggtccgcc atataaagtg ttgacacgga
     9601 tccttaatcc ttttttccct tccatccggt ctttaatctt atccagtttg aaagatacat
     9661 atccttcgcc caggctttga ggataatcac cttcactgtt atggcggaat tccagattga
     9721 tatattcgtc ctccgcactg tcttcgtcag cagtgggagc agactcttct ttgtcgttga
     9781 ttacgagatt caggaaatgt tttttatctt cactatgcgt tccataatat tggaattcaa
     9841 tcgtcagata tttattatcc ttgttgatcc acatataagt tgtgttgatc ttgtcatctc
     9901 cgactttttc ttcgtcgttg tcatcttcac ccatcgtgat gatgtcttta gttaagattg
     9961 gattgatacc tctcacctga atattaaggt cgtatccatt cacaggctct tccagctcgt
    10021 tgaatatcac aaaagctcgt tgtccttcca cccaatcttt attactccat ccttgcgcat
    10081 tgctgggata catctttttc ccgtcatcca aagtgaaata aaattccttg ctatcctggc
    10141 taatcatatt gatggtactg acaaccagaa gatcggacgg gttgtcatcg tcgtccaaac
    10201 atgactggag aatcggcatt gtagtcataa tagcaaagat aaaggcaatg aatttaaatt
    10261 tcttcatata cttatttaag ttttagatta tataaaatgg ttcctttgta ttgtgaaaat
    10321 gcagtggaat agaaaatatt gcatttgaat ctgttaaaga aacaaaagtg ataaaagagt
    10381 tgttctctct atgcagtatt tctatctttg ctgcatttat aagttaaaca cgtgtataag
    10441 agaatggaag aattagagtt gtcggaacaa tgcaggctag gcaataacca agctcgcaaa
    10501 gaactgtatg agcagtatgc gggacgtatg cttggcatct gtcttcgcta taccggcgac
    10561 cgtgatacag cccaggatct gcttcacgac ggttttttaa agatattcga ttccttcgac
    10621 aagttcacct ggaggggtga aggctccctg cgggcctgga tggaaagggt gatggtcaac
    10681 acagctttgc aatatctccg gaaaaatgat gtaatcaatc agtcggcacc attagaagag
    10741 ttgcctgaag aatatgaaga accggatgct tctgatgtag aagctattcc acagaaagtg
    10801 ttgatgcagt ttatagaaga actacctgcc ggatatcgta cagtgtttaa cctttataca
    10861 ttcgaagaca aatcgcataa agaaatcgca caggaattag gtattaatga aaaatcttcg
    10921 gcttcgcaac tatttcgtgc gaagagtgta ttggcaaaaa gagtaaaaga atggataacg
    10981 cataatggat agatagagat ggaagagaaa gaattgtgga tgaataagtt gaaggagaag
    11041 ctcgcggatt attccgagcc ggtgcctgct tccggttggg aacaactgga gaaagaactt
    11101 atgcctcctg tggagaagaa aatatatcct tatcgaaaat ggatgatggc tgctgcagct
    11161 gttatattgt tagctgtcgt ttcgtcggta agtctatatt ttctggggac accggctgcg
    11221 gatgaaatac gtcatataaa gactccggca ttggcatcta cacccgatgc gttgccgggt
    11281 gtgcaacaac cagatgtgca gggcacttcc gtagaacccg tcttgcgacc agtggctcgt
    11341 gaagaccgtt tggcgaaaat tgataagaat cttacagaac agaaaacaaa tgcaggtcaa
    11401 tctgcaatag aaaataacaa cgaacctgta acagggaatg aaaataatcc tgtgattgag
    11461 gaagaccaga tgctcaaagg tgagacggaa cagacgaaaa atgaagcaag gcaggttgac
    11521 agtgaaaatg tgggtcaggc tacacagtca caggacaccg aacggccgaa caacaggccg
    11581 cgtcgccctt ccggcagaga caagctgcat attccggcag aaaaaagagc atctcaaaaa
    11641 ggaacatggt ccatgggact ctcggtcgga aattcgggcg gagcatccac agaagtcggt
    11701 gccggctctc atgcttatat gtctcgtgtg agtatgcttt ctgtttccaa cggtctgatg
    11761 gagataccta acgatcagac attggttttt gaagatggag tgccttatct gcgtcaggca
    11821 aaacaagtgg tcgacattaa gcaccatcaa cctatttctt tcggattgtc tgtccgtaaa
    11881 ggactggcca aaggattctc tctcgaaacg ggattgactt acacattgct ttcttcggat
    11941 gcgaaactgg cgggtgaaga tcagcagata gaacaaaaac ttcattatgt cggtattccg
    12001 ctgcgtgcta actggaattt ctttgataaa aaactcgtca ctctctacgt ctccggtgga
    12061 ggtatggtgg agaaatgtgt ttatggaaag ctcggttccg agaaagaaac ggtgaaacct
    12121 ttgcaattct cggtatctgg tgcggtaggt gcacaggtga acgcaacgaa acgtctgggt
    12181 atctatgtag aacccggtgt tgcttattat tttgatgacg gttcggatat acagaccata
    12241 cgtaaagaga atccgtttaa cttcaatata caggctggta tccgtttgac ttattaatgc
    12301 gaattaccat actgaacata gtgacggtat ccgttcaaga tagcgtcact atcctgatta
    12361 gatagcgtca ctatcttgac cggatggcgt cactatcctg atattagtat ctttttcctc
    12421 ataaaaatcg gataattgag aaaatattaa taatattgtc acctgttaat ggtctatggt
    12481 tgcatacctt tgttgcaaaa ttctaagcta tggtgaattt tacagatgaa aaacatttat
    12541 taatcgattt aaaagacggt tcttttcaag catttgaaag attgtataat atgtacagcg
    12601 gtaaactgta taatttcatt atgcggcttt cttccggcaa tcaatatatg gctgaagaag
    12661 tggtgcaatc cacttttatc cgtatatggg aagtacgtga gaaagtcgat acgaacgctt
    12721 catttatttc ttttctttgt acgatagcca aaaatttact gatgaacatg taccagcgtc
    12781 agaccgttga atatgtttac aatgaatatc tgctgaaaag cagtgtggat cgtgattcgc
    12841 aaacagaaga gaatattgat cttcatttct tgaacgacta tatcgactca ttagcggaag
    12901 aattgccggc acagcgaaag aaaatcttta tcttaagtaa gcgtcagaac tatacgaata
    12961 aggaaattgc tgaaatgatg ggtatttctg aaagtacagt cgccacacaa ttatctttgg
    13021 cggtgaaatt tatgcgtgag cagttgatga agcattacga taaggtaatt gcgcttctga
    13081 tcgccttttt tgttaatgaa atgtaattgt aatacatgtt tttggatagt agataggatg
    13141 aaaatgtata cttgtaaata agcaggaaag aagatggata agatatatta taaagaactg
    13201 attgaaaagt atttcgatgg aaatataacg gatgcggaga ttaaagaact ttccgattgg
    13261 ataaagaacg accgccattt acagaactgg tgggaagaag aattttccaa atcggatgcc
    13321 ggtattaatc cggtactgcg cgacaaacta tttgcccgaa tcaaagaaca gacgcaggga
    13381 aaagaagaga cacaggaaaa agagaaacca agaactattc gcatgaatcc ttggaaatgg
    13441 gctgccgcca tcgttttacc catttgtatt gccttcttca cctactatct tatagattct
    13501 tcccaaacag tgggagcacc ctttatagta aaagctgata aaggagataa agcaaccatc
    13561 gaattgccgg acggaacgaa tgtcgttctc aattctgctt cgcaattaag ctatctgaat
    13621 aacttcggag aaaatgtgcg tcgtgtgcaa ctgaatggtg aagcctattt caaagtagct
    13681 catgatgaaa aacatgcctt tatagtgcag gtcggtgatt tggaagtgaa agtactcggt
    13741 acttctttta atgtatctgc ctatgaagat gccaaagacg tgacagtcgt tcttttggaa
    13801 ggaaaagtag gggtctatgc gcagaaaatg tcgcatatca tgaagcccgg cgacaaaata
    13861 gaatataata aagccactca taagataaca gccactcaag tacacccgag tgactacatt
    13921 gaatggacga aaggaaatat ctatttcgag aaagaatctt tggagaatat aatgaaaacc
    13981 ctttcacgca tctatgacgt agagattcgt ttcgactcca ataaactacc taatgaatat
    14041 ttcaccggaa ccattccggt tggtggcata cagaatgcat tgaatatcct gatgcttact
    14101 tcacctttct attatgaaat ggacggttcg gtgattgttt tgaaagagaa ataagtggaa
    14161 ctttcataga gtatctttgt ttattcatcg aataacgtct atatttgtgg tacactaatt
    14221 tgaagatgca atgaattcat tggaacgtaa attgccgata gggatacaga catttgaaaa
    14281 gatgcgtgca gaggggtgtt tgtatgtgga taaaacagcc attatatatc agatagcagc
    14341 tactaaagtg ccttattttc ttagtcgtcc ccgtcgtttt ggcaaaagtc tgctaatttc
    14401 cacttttgaa gcctattttc agggtcgtaa agaccttttt gaaggcttgg caattgaaaa
    14461 gttagagacg aagtgggagc aatatccggt tctgcatctt gatttgaatg cgaagaaata
    14521 tgagacggcg gcagatttgg ttgccatgct aaaccagtat ttggagaaat gggaagccgt
    14581 atatggtgat gagaagaaag accgtagtcc tgaagaacgt tttagttata tcattgagca
    14641 agcctatctg aaaacaggaa aaggagttgt ggtattggtt gatgagtatg ataaaccttt
    14701 gctgcaagcc cttttagatg agaacctgtt ggatgaatac cgtcgtattc tgaaagcctt
    14761 ttatggtgta ctgaaaagtt ccgaccgcta tcttcggttt atattcttga caggagtcac
    14821 aaaatttgcc caagtaagtg tgttcagtga tttgaatcag ctgaatgata taagtatgaa
    14881 aattccatac gccaatattt gtggtattac taaaaaggag ttag
//
