LOCUS       gnlProkkaIBOLCLPD_196   6710 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IBOLCLPD_196.
ACCESSION   gnlProkkaIBOLCLPD_196
VERSION     gnlProkkaIBOLCLPD_196
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 16:28:21
            Original ID  :: gnl|Prokka|IBOLCLPD_196
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6710
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6710
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[304:3196]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      305..3196
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..6710
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..6710
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             2..115
                     /locus_tag="ctg196_1"
                     /transl_table=11
                     /translation="MLEELQKDGTLAKLAEKFFGNKDYIPTKDALKVPSKK"
     CDS             305..3196
                     /NRPS_PKS="Domain: AMP-binding (6-388). E-value: 4e-65.
                     Score: 211.8. Matches aSDomain:
                     nrpspksdomains_ctg196_2_AMP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     172.2; E-value: 2.5e-52)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg196_2"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-65, bitscore:
                     207.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.8e-07, bitscore:
                     20.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILKTFSDTVKQYPSKVAVMYSGGEFTFQEVDVMSNFIAKQLLL
                     NSDEETVPFYIEKNKYVLPVVLGIMKSGKIPLPITNSLEVKISLERISEVVFDVLISDR
                     DVKLENHSVTLLLLSKKLESYSEYQEVATTKENEIAHIICTSGTTGVPKKVFLTDDNID
                     WLVKEFYKIVKFTSESKFLFTTPYTFDVSLTEILAPVYTGGTLVCYDGGIQNIIRLGET
                     IEKKKITHLSLSPSFAETIIDISGPEVFQNLRALCLAGETFPSSLANRLRGLIQQGCRV
                     FNLYGPSETTIYATYYELKDRKYNTVPIGKPLPGVQLKIASQKENSKMAELLIGGNGVT
                     EGYRLQPELNKAKFKLIGSNRYYVTGDYVYYQGDNLVFSSRIDSQVQVNGIRIELDEVK
                     SIVDKLKSVKSSRVVFYKKKLVVFYEADLNIKEDIIRSLPSYLNPTVVKVESYYLNQNR
                     KLDVPKMLEIYYYKKQSQQGDDVRKNILDVLSKFERVDITDLDSLELVRFYLEIEDIFD
                     IEIKENDFYRLKSVDSIVDYIRNKSFFQERSTEPNDSFTKEHDLVNLEYNLTKMNYRYL
                     KNIITPSPTQKRLYKNNQNRVIGFNLALEEVTYLELNKLHHIIKYLSYKIDIFRLVVEK
                     EQTRFSIGIVNEGDYSPNIIVLNNLPTELELQGILDNIEVIPIPIIVVGTRDKKVRFYF
                     PYHSIDASSLNKLGDTVYQLYEKKIVIDEVPSSSLVAFSQFKREVLQNDVSGDVLARLP
                     KIKPAIEFEKLNDEVQCFQVSLPEDIKGDDVYLFTIYAISQCILSDYNLSAISGGLSLD
                     FRDYEQFDAYDLIGDIHKKIPFQVNRTETFEEFKNSHFKILEIYRTGIDYYEIAMLGND
                     MSAKEVQERLNNLSLSINFIGEAFRLKDTINNIIDVDFDKNFINVFVHERFAYAVIKSE
                     LLSNSVYDLMHKDSKFELKVIDKKRLENEETNR"
     aSDomain        323..1468
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg196_2_AMP-binding.1"
                     /evalue="4.00E-65"
                     /label="ctg196_2_AMP-binding.1"
                     /locus_tag="ctg196_2"
                     /protein_end="388"
                     /protein_start="6"
                     /score="211.8"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FSDTVKQYPSKVAVMYSGGEFTFQEVDVMSNFIAKQLLLNSDEET
                     VPFYIEKNKYVLPVVLGIMKSGKIPLPITNSLEVKISLERISEVVFDVLISDRDVKLEN
                     HSVTLLLLSKKLESYSEYQEVATTKENEIAHIICTSGTTGVPKKVFLTDDNIDWLVKEF
                     YKIVKFTSESKFLFTTPYTFDVSLTEILAPVYTGGTLVCYDGGIQNIIRLGETIEKKKI
                     THLSLSPSFAETIIDISGPEVFQNLRALCLAGETFPSSLANRLRGLIQQGCRVFNLYGP
                     SETTIYATYYELKDRKYNTVPIGKPLPGVQLKIASQKENSKMAELLIGGNGVTEGYRLQ
                     PELNKAKFKLIGSNRYYVTGDYVYYQGDNLVFSSRIDSQVQV"
     CDS_motif       722..778
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg196_2_0001"
                     /evalue="1.00E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg196_2"
                     /protein_end="158"
                     /protein_start="139"
                     /score="15.1"
                     /tool="antismash"
                     /translation="AHIICTSGTTGVPKKVFLT"
     CDS_motif       1295..1366
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg196_2_0002"
                     /evalue="2.80E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg196_2"
                     /protein_end="354"
                     /protein_start="330"
                     /score="19.7"
                     /tool="antismash"
                     /translation="ELLIGGNGVTEGYRLQPELNKAKF"
     CDS_motif       1442..1498
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg196_2_0003"
                     /evalue="2.60E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg196_2"
                     /protein_end="398"
                     /protein_start="379"
                     /score="20.0"
                     /tool="antismash"
                     /translation="SRIDSQVQVNGIRIELDEV"
     CDS_motif       2078..2125
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg196_2_0004"
                     /evalue="1.30E+01"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg196_2"
                     /protein_end="607"
                     /protein_start="591"
                     /score="-0.8"
                     /tool="antismash"
                     /translation="NNQNRVIGFNLALEEV"
     CDS             3193..3432
                     /locus_tag="ctg196_3"
                     /transl_table=11
                     /translation="MSIYKDIVEGYQLNNIQEKDKLNNVLVQLNEKDDQEMINRKNFIG
                     HFTASAFVISKDNRRLLMVHHNILKRYLQPGGAY"
     CDS             3560..4840
                     /locus_tag="ctg196_4"
                     /transl_table=11
                     /translation="MHKIPENKLKNERSHYHYDLQYLFYIENEVDVKIDSNESSSYEWI
                     QLDNVKDMDGYKEIIKKIEKLEAASQERFLSNMLKGASFEDISCITVQHIIPSTIPIIM
                     TLQKIFGHRLLVCAKPNSVDKMVWHKLENMGVRLTLASRDEYFNVDYLGIKSKTILLDI
                     GGYFASIAQIPNLPIECIIEDTENGIQKYENVIDQIEYPLFSVARNPLKKNEDYLVGAD
                     IVFGTDYILHQKNLLMQYMQVVCIGYGKIGYGICTKLRELGIRPKVLEKDSMRTIQAVR
                     DGCDILLEKDFKNIDLIFCATGSKSLDILDFRSIKDGTFLVSATSSDDEFNYSYLLDEY
                     EEIVETSLITRYESEDNYFYLLNQGTPTNFVVNSALGNYILLVQAAILYTAKKFIEDRE
                     MAIAKQVNTLSDEDNYNIAKQWLEEFC"
     CDS             4866..6074
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 278.7; E-value: 1.3e-84)"
                     /gene_kind="transport"
                     /locus_tag="ctg196_5"
                     /transl_table=11
                     /translation="MNKKEQQNTYLLVGSRIISRVGDIMFDFANNTFLSSVNVKSLMLV
                     GIYQTLENIISVIFNLFGGVLSDNFQRKRIIILTDILSGVLCLALSFISNQQWLIYAVV
                     ITNIILAFFSSFSGPAYKAFTKEVVEADNISKLNSLLESFITVVKVVVPLVSVSLYGLL
                     GLYGILRLDGITFIASGLLILFIRPILEESTGKQVFSLSKIFSDLFEGGRYLASQKEIL
                     YLVILSAIVNFFLAAYNLLLPYSNQMFPRVTVGLYGVFLGAEAVGGLIGASLSSILNKV
                     LKIKTMILYLGVSGVFLSLTPMVYCVFPSAYMLALYPALFNLFLTMFNLQFFSYIQKNV
                     DNVFLGRVFGIIFTVAILFMPIGTMTFTFLLSPKNIFNFVIVGLSIGGISLLFYRIFSK
                     RLR"
ORIGIN
        1 acgtttggaa gaactccaaa aagatggtac tttggctaag ttggctgaaa aattctttgg
       61 aaacaaagat tatattccaa ctaaagatgc tttgaaagtt ccaagcaaaa aataagaaag
      121 tctcttttgt caactgtagt cggtgacgaa aagcctttgt ttattatatg ataaataaaa
      181 gagggaaatg atgaaaaagg taaaattttt gccaactttg aaaattgctt ttgaaattct
      241 tattgttgtg tgtggataaa ttatgaatga cagtgcataa tcaatcaaat taaggagaag
      301 agaaatgacc atattaaaaa cttttagtga tacggtgaaa caatatcctt ctaaagttgc
      361 tgttatgtat agcggaggag agtttacttt tcaagaagta gatgtgatgt ctaattttat
      421 tgctaagcag ctactactca atagcgatga agagactgta cctttttata tagaaaaaaa
      481 taagtatgtt ttaccagttg ttttggggat aatgaaatcg ggtaaaattc cgttacctat
      541 aacaaatagt cttgaggtca aaatatcatt agaacgtatt agcgaagttg tctttgacgt
      601 gttgataagt gatagggatg ttaaacttga aaatcattca gtaactcttt tgctcttatc
      661 caaaaaatta gaaagttaca gtgagtatca agaagtagct acaactaaag agaatgaaat
      721 tgcacatatt atctgtactt ctggaactac tggggttcct aaaaaagtat ttttaactga
      781 tgataacata gattggcttg taaaagaatt ttataaaatt gtgaagttta cttccgaatc
      841 aaaattttta tttacaactc cctatacttt tgatgtttca ttaacagaga ttttggcacc
      901 tgtatatacg ggtggtactt tggtttgtta cgatggtggg attcagaata ttattcgact
      961 aggagaaaca attgagaaaa agaagataac tcatttatca ttatctccgt cttttgctga
     1021 aactattatt gatattagtg ggccagaagt attccagaat ttaagagcgt tgtgtctagc
     1081 aggtgagaca tttcctagtt cattagcgaa taggttgaga gggcttattc agcaagggtg
     1141 tcgagtattt aatttatatg ggccttcaga aacaacaatt tatgcaacct actatgagtt
     1201 gaaagataga aaatataata ctgttccaat tggtaagcct ttgcctgggg ttcaactaaa
     1261 gatagcttct caaaaagaga attctaagat ggcagaactt ctaattggtg gtaatggagt
     1321 aacggaaggg tatagactac aacccgaatt aaataaagct aagtttaagt tgataggttc
     1381 aaataggtac tatgtaacgg gggactacgt ttattaccaa ggagataact tggtatttag
     1441 ttcaagaata gatagccaag tccaagtaaa tggtattcgt attgaattag atgaggtaaa
     1501 atctattgta gataaactta aatcagttaa atcatcacga gttgtttttt ataagaagaa
     1561 acttgtcgta ttttatgaag ccgatttgaa tattaaagaa gatattatta gaagtttacc
     1621 tagttactta aatccgacgg tagttaaagt tgagagttat tatctcaatc agaatagaaa
     1681 gttggatgtt ccaaaaatgc ttgagatata ttattataaa aaacaaagtc aacaaggaga
     1741 tgatgttaga aaaaatattt tagatgtact atctaaattt gaaagagttg atatcacaga
     1801 tttggattcg ttagaattag ttagatttta tttagagatt gaggatatct ttgatattga
     1861 aattaaagaa aatgattttt ataggctaaa gagtgtagat tcgattgtgg actatattag
     1921 aaataaatca ttttttcaag aacgttcaac tgagccaaac gatagtttta ctaaagaaca
     1981 tgacttggtt aatttagagt ataatctcac aaaaatgaat tatcgttatt tgaaaaatat
     2041 tattactcct agcccgacac aaaagagact ttataaaaac aatcaaaatc gtgttattgg
     2101 ttttaattta gcactggaag aagtgactta tttagaattg aataagctgc accatattat
     2161 taaatattta agctataaaa ttgatatatt tagacttgtt gtcgaaaagg agcaaactcg
     2221 atttagcata ggtattgtga atgaaggaga ttactcacct aatattattg tactaaataa
     2281 tttaccaaca gagttagaat tacaaggaat attagataat atagaagtga ttcctattcc
     2341 aattattgtg gtgggaacaa gagataaaaa agtaagattt tattttccat atcattctat
     2401 tgatgcatca tcattaaata aacttggaga tacagtatat caactgtacg aaaaaaagat
     2461 agttattgat gaagtaccta gtagttcact ggttgctttt tcgcagttta aaagggaggt
     2521 gttacagaat gatgtctctg gggatgttct tgctagattg ccaaagataa agcctgctat
     2581 tgagtttgag aaattaaatg atgaagttca atgttttcaa gtttctttac cagaagatat
     2641 taaaggagac gatgtatatc tttttacaat ttatgctatc tcacagtgta ttttaagcga
     2701 ctataatctg tctgctatat ctgggggtct atctttggat tttagagatt atgaacagtt
     2761 tgatgcgtat gacttaatag gagacattca caagaaaatt ccattccaag taaatcgaac
     2821 tgaaactttt gaggagttta aaaactctca tttcaaaatt ttggagatct atcgtacagg
     2881 tattgattac tatgaaattg caatgttagg aaatgatatg agtgctaaag aagtgcaaga
     2941 acgattaaat aatctgtctc tatcaataaa tttcattggc gaagcattta ggttaaaaga
     3001 tactattaat aatataattg atgttgattt tgataaaaat tttattaatg tatttgtaca
     3061 tgagcgattt gcatacgcag ttataaaatc tgagttattg agtaatagtg tttatgactt
     3121 aatgcataaa gatagcaaat tcgagctaaa agtaattgat aaaaaacgac tggaaaatga
     3181 ggagacaaac cgatgagtat ttataaagat atagtagagg ggtaccaact gaataatatt
     3241 caggaaaagg ataaattgaa taacgttttg gtacagctta atgaaaaaga tgatcaagag
     3301 atgataaata gaaagaattt cattgggcat tttacagcat cagcatttgt tatttcaaaa
     3361 gataacaggc gtttattgat ggttcaccat aatattttaa aaagatacct acagcctggg
     3421 ggggcatatt gataaaaatg atagaaaccc gctagatgca gcgaaacgtg agctgtttga
     3481 ggagactggg atagattctg aaatattgac gtatcaatgt gcagaaccat tgaatagctt
     3541 gatacctttt aatgtttctg tgcataagat accagaaaat aaattgaaga atgagcgttc
     3601 ccactaccat tatgacttgc aatatttatt ttatattgaa aatgaggttg atgtgaaaat
     3661 tgacagcaac gaatcgagtt cctatgagtg gattcagcta gataatgtaa aggacatgga
     3721 cggctataag gaaatcatta agaagattga aaaacttgaa gcagcttctc aagaacgttt
     3781 tttatctaat atgctgaaag gtgcatcgtt tgaagacata tcatgtatta ctgtacaaca
     3841 tattattcca agtacaatac cgattatcat gacattgcaa aaaatatttg ggcatcgttt
     3901 gttagtgtgt gctaaaccaa actctgttga taagatggta tggcacaagc tagaaaatat
     3961 gggggtaaga ttaacattag cttcaaggga tgaatatttt aatgtagatt atctggggat
     4021 aaagagtaaa acaattttat tagacattgg tgggtatttt gcctcaattg ctcagattcc
     4081 aaacttacct atcgagtgta tcattgaaga tacagaaaat gggattcaaa aatatgagaa
     4141 tgttattgat caaatagaat atccgttatt ttctgtcgct agaaatcccc tgaagaagaa
     4201 tgaggattac ttagttggtg cggatattgt ttttgggaca gattatatat tacatcaaaa
     4261 aaatttatta atgcaatata tgcaagtggt ttgtattgga tatgggaaaa tcggttacgg
     4321 catctgtacc aaattaagag aacttggtat tagaccgaag gtgctggaaa aagatagcat
     4381 gagaactatt caagcggtac gtgatggttg cgacattttg ctagaaaaag atttcaagaa
     4441 tattgatttg attttttgtg cgaccggttc aaaaagtcta gatattttag attttcgctc
     4501 tatcaaagat ggaaccttcc tagtctctgc aacatctagt gatgatgaat ttaattatag
     4561 ctatttattg gatgaatatg aagaaatagt cgaaacttca ttaatcacta gatatgaaag
     4621 tgaagacaac tatttctatc tgttaaatca aggaacacca actaattttg tagtaaactc
     4681 cgctttggga aattatattt tattagtcca ggcagccatt ctatatacag ctaagaagtt
     4741 tattgaagat agagaaatgg ctatagctaa gcaagtaaac actctttcag atgaggataa
     4801 ctataatatt gcgaagcaat ggttagaaga gttctgctaa tgaacaaata tctgaggagt
     4861 ttattttgaa taaaaaagaa caacaaaata catatttact tgtagggagc cgtattattt
     4921 ctcgtgttgg agatattatg tttgactttg cgaataacac ttttttatcg agtgtaaatg
     4981 ttaagtcact tatgttagtt ggtatttatc agacattaga aaacatcatc agtgtgattt
     5041 ttaatctttt tggtggtgtt ctgtctgata actttcaaag aaaacggatt atcattttaa
     5101 cagatatttt aagcggagta ttgtgtcttg ccttatcttt tatttctaat cagcagtggt
     5161 tgatttatgc tgtggttatc acaaacatta tattagcttt tttctcatca ttttcaggcc
     5221 ctgcttataa ggcttttact aaagaagtgg tggaagcaga taatatttca aaactaaact
     5281 cattactaga atcgtttata acggtagtta aagtcgttgt tcccctggta tcggtatctc
     5341 tatacggttt actaggatta tatggtattt tacgtctaga tgggataaca tttattgcat
     5401 ctgggttgct tattctcttt attcgtccca tacttgaaga aagtactggg aaacaggtgt
     5461 tttcattatc gaaaatcttt tcagatcttt ttgaaggagg aaggtatcta gctagtcaaa
     5521 aggaaatttt gtacttggtt attttatctg cgattgttaa tttcttttta gcagcttata
     5581 atctgttgtt accatatagc aatcagatgt tcccaagagt taccgttggc ttatacggtg
     5641 ttttcctagg agcagaagca gtaggtggtc ttattggagc gtctttaagc agtattctca
     5701 ataaagtttt gaaaataaaa acaatgattt tatatcttgg agtatcaggt gtttttctat
     5761 ctttgacacc tatggtatac tgtgtttttc caagtgctta tatgcttgcg ttatatcctg
     5821 ctttattcaa cttgtttttg actatgttca acttacagtt tttttcatat atccaaaaga
     5881 atgtggataa tgtctttctt ggtagagttt ttgggattat ttttactgtt gcaattttat
     5941 ttatgcccat tgggacaatg acatttactt ttcttttaag ccctaaaaat atctttaatt
     6001 ttgttatcgt tggtttatct ataggaggta tttcgcttct cttttatagg atttttagca
     6061 aaagattgag gtaaattttc agagtaattt tagctccgat ggcgtagtaa aattgcttag
     6121 taaagtcaca atttatatac ttgttagggc aaccattaca cacataaggt aatcgtttta
     6181 gaagtgggcg ggcctcacat tttgagtgtt gattgattcc ttgatggtaa ggtgtttaca
     6241 tactttttta gagacagtag atgggtcgcc gattagttta gaaatagctc taaatgtctt
     6301 gttcgtttct cataaaagtc agcgatattg cagggattgg tatgactagc tgaagcgatg
     6361 ttgtgaatgt acttgaacaa gattttactg gcatagggat tccctcgttt agtgatgtgt
     6421 tccttagcga gaaagttccc agattcatag tgtctcaggt caatgccaat aaaagcgttg
     6481 atttgattgg tatactgcga cgaatatctc ccaattcgcc aatgatagat gtcgctgtgg
     6541 tttctgcgat gccaggaatt gaccgtaaga tgtcatactc tggtaaaggc tgagctagag
     6601 ctaccatgtc gtttaagaca acccgtctgc gttcagaaag acgaagcaat tcggctcttt
     6661 gtcaaacaat tgatattttt aggtgatagt attttcttat cactttgatt
//
