LOCUS       gnlProkkaBPKMPIEC_144  13038 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BPKMPIEC_144.
ACCESSION   gnlProkkaBPKMPIEC_144
VERSION     gnlProkkaBPKMPIEC_144
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 17:20:44
            Original ID  :: gnl|Prokka|BPKMPIEC_144
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13038
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13038
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2798:10681]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2799..10681
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     cand_cluster    1..13038
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13038
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     CDS             complement(258..1166)
                     /NRPS_PKS="Domain: PKS_ER (2-209). E-value: 3.2e-12. Score:
                     38.0. Matches aSDomain: nrpspksdomains_ctg35_1_PKS_ER.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 198.5; E-value:
                     2.2e-60)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg35_1"
                     /sec_met_domain="ADH_N (E-value: 5.5e-12, bitscore: 35.3,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2e-22, bitscore:
                     69.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MILGHEACGVVVEVGEDVKDFKPGDRVLVPAITPNWSDVYSQAGY
                     ATDSDGMLGGWKFSNIKDGVFATRFHVNDADGNLAHLPDEIDPAEACMLSDMIPTGLHA
                     SEMAGVTFGDDVAVIGIGPVGLMALRGAVLHGAGRVFAVGSREKTVEVAKKYGATDIID
                     YHNGRISDQILEATGGQGVDKVLIAGGNAKDTFEEAVRMLKPGGSIGNVNYLNGHDDVV
                     FNAGDWGVGMGHKTIHGGLMPGGRLRMEKLAVLVVNGRIDPSLMITHRFEGFEKIPDAI
                     ELMHHKSADLIKPVVICNDID"
     aSDomain        complement(540..1160)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg35_1_PKS_ER.1"
                     /evalue="3.20E-12"
                     /label="ctg35_1_PKS_ER.1"
                     /locus_tag="ctg35_1"
                     /protein_end="209"
                     /protein_start="2"
                     /score="38.0"
                     /tool="antismash"
                     /translation="LGHEACGVVVEVGEDVKDFKPGDRVLVPAITPNWSDVYSQAGYAT
                     DSDGMLGGWKFSNIKDGVFATRFHVNDADGNLAHLPDEIDPAEACMLSDMIPTGLHASE
                     MAGVTFGDDVAVIGIGPVGLMALRGAVLHGAGRVFAVGSREKTVEVAKKYGATDIIDYH
                     NGRISDQILEATGGQGVDKVLIAGGNAKDTFEEAVRMLKPGGSI"
     CDS_motif       complement(735..761)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg35_1_0002"
                     /evalue="5.30E+00"
                     /label="PKSI-ER_m2"
                     /locus_tag="ctg35_1"
                     /protein_end="144"
                     /protein_start="135"
                     /score="0.9"
                     /tool="antismash"
                     /translation="GAGRVFAVG"
     CDS_motif       complement(1122..1160)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg35_1_0001"
                     /evalue="6.00E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="ctg35_1"
                     /protein_end="15"
                     /protein_start="2"
                     /score="9.7"
                     /tool="antismash"
                     /translation="LGHEACGVVVEVG"
     CDS             complement(1181..1327)
                     /locus_tag="ctg35_2"
                     /transl_table=11
                     /translation="MKGFAMLGPNKTGFIEKEDPKVGSRDALVRPLAVAPCTSDIHTVF
                     EDA"
     CDS             complement(1558..2748)
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 230.9; E-value: 3.3e-70)"
                     /gene_kind="transport"
                     /locus_tag="ctg35_3"
                     /transl_table=11
                     /translation="MDAKPNRRALSLGSFVILYLGYMLLFADRTVLNLSLAAIGKDFSV
                     SPAALGATSSVFFLGYTIMQIPGGWLTDRFSSRKVIIATLAFWSVMTIFTGLAWSLAAL
                     LVIRFLFGIAEGPYPPAAMKQVAQDFPVNKRAQLTAALISSNYAGAALAPFIIVPIIAA
                     TGWRSAFFWLGGFGLLLMVSYLFFRGHGGGQTVTKQERQKINWKEIDPRVWAFVVIGLA
                     LNIITKGMETWMPVYFLNARHINLANLAWLVPLPTIAGGIAALISGYVMVHIFKNHERL
                     MIILASFLTVLTMFGLYKSTSLVWLVIFQMATYFVKSLAFTGIFSYAAQLFPQKTYGSQ
                     IGTINFGGQLGGFFGPLLIGWIVQLSGSYDTAFFGLVISALVAGVASLFIGNLKAK"
     CDS             complement(2799..4223)
                     /NRPS_PKS="Domain: AMP-binding (8-379). E-value: 2.4e-71.
                     Score: 232.3. Matches aSDomain:
                     nrpspksdomains_ctg35_4_AMP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     307.2; E-value: 2.9e-93)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_4"
                     /sec_met_domain="AMP-binding (E-value: 4.4e-76, bitscore:
                     245.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIPNWLLKQASLNPNRVAVNDGTTAYTFAQLVPLVAHRAGQIQA
                     QTDQPRVGVMTHNNLAGYLAALGVLAADKTIVWLNWRLAPEELSRQIGDAGLDLVLVDD
                     QLADRYYGGHRVLCSELTAGTAPAAVLTPTVDLDAVASIMYTSGTTGQPKGVLQTYGNH
                     LASAISSSLNLGVLPNDLWLCVAPIFHISGFSILMRGLIYGMGVRLVNHFDAQVVNQIL
                     EDEPVTIISAVPYMLKKMLEDVQANDHCYSSAFRLVLLGGGTIDRATLNACEESGLPIV
                     QCYGMTETCSQFVALSAEEAPYHLGSVGKPLFTTQLRLVGEHHEIEIQTPALTPGYLNQ
                     PAKFAAKHDGDWYRTGDVGHFDEDGFLYVDGRLDEMIISGGENIFPAEVENVLRQYPGI
                     QEIAVVGRNDQQWGAVPVAVVVSKQPVDFQQLEAFGREHLAHYKVPKVYLQTDALPKNA
                     SGKVQKFRLQDLVANA"
     aSDomain        complement(3087..4199)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg35_4_AMP-binding.1"
                     /evalue="2.40E-71"
                     /label="ctg35_4_AMP-binding.1"
                     /locus_tag="ctg35_4"
                     /protein_end="379"
                     /protein_start="8"
                     /score="232.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="KQASLNPNRVAVNDGTTAYTFAQLVPLVAHRAGQIQAQTDQPRVG
                     VMTHNNLAGYLAALGVLAADKTIVWLNWRLAPEELSRQIGDAGLDLVLVDDQLADRYYG
                     GHRVLCSELTAGTAPAAVLTPTVDLDAVASIMYTSGTTGQPKGVLQTYGNHLASAISSS
                     LNLGVLPNDLWLCVAPIFHISGFSILMRGLIYGMGVRLVNHFDAQVVNQILEDEPVTII
                     SAVPYMLKKMLEDVQANDHCYSSAFRLVLLGGGTIDRATLNACEESGLPIVQCYGMTET
                     CSQFVALSAEEAPYHLGSVGKPLFTTQLRLVGEHHEIEIQTPALTPGYLNQPAKFAAKH
                     DGDWYRTGDVGHFDEDGFLYVDGRLDEMIIS"
     CDS_motif       complement(3753..3803)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg35_4_0001"
                     /evalue="4.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg35_4"
                     /protein_end="157"
                     /protein_start="140"
                     /score="22.4"
                     /tool="antismash"
                     /translation="ASIMYTSGTTGQPKGVL"
     CDS             complement(4233..5054)
                     /NRPS_PKS="Domain: ECH (18-261). E-value: 4.2e-63. Score:
                     204.7. Matches aSDomain: nrpspksdomains_ctg35_5_ECH.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 197.1; E-value:
                     4.6e-60)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg35_5"
                     /transl_table=11
                     /translation="MTSANWTPVKEYSEIIFERDDKIAKITMNRPEKHNAFTPVTVAEM
                     INAFNICRDDSTIGVIILTGAGDKAFSSGGDQSVRGNGGYVGPDHIARLNVLDLQHLIR
                     IIPKPVIAMVKGWSVGGGNVLQLVCDLTIAADNAMFGQTGPKVGSFDAGYGSGYLARVI
                     GHKRAKEVWFLNHFYTADEAYQMNWINKVAPLAEVESVTLDWCHEILKKSPTALRFIKA
                     AMNADTDGLAGLQQFAGDATMLYYTTDEGKEGRDAFNQKRDPDFDKFPKFP"
     aSDomain        complement(4272..5000)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg35_5_ECH.1"
                     /evalue="4.20E-63"
                     /label="ctg35_5_ECH.1"
                     /locus_tag="ctg35_5"
                     /protein_end="261"
                     /protein_start="18"
                     /score="204.7"
                     /tool="antismash"
                     /translation="RDDKIAKITMNRPEKHNAFTPVTVAEMINAFNICRDDSTIGVIIL
                     TGAGDKAFSSGGDQSVRGNGGYVGPDHIARLNVLDLQHLIRIIPKPVIAMVKGWSVGGG
                     NVLQLVCDLTIAADNAMFGQTGPKVGSFDAGYGSGYLARVIGHKRAKEVWFLNHFYTAD
                     EAYQMNWINKVAPLAEVESVTLDWCHEILKKSPTALRFIKAAMNADTDGLAGLQQFAGD
                     ATMLYYTTDEGKEGRDAFNQK"
     CDS             5089..5472
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144:thioesterase superfamily protein (Score: 100.2;
                     E-value: 1.1e-30)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg35_6"
                     /transl_table=11
                     /translation="MNLLENLGIHQDLVTKDRVELSMPVQAKHLQPYGIVHGGINTVLA
                     ETAASLGANANVAPGRIAVGVNVTTQHIRAIREGEIFVVATPDHVGRTLQTWQARLLVN
                     DRLTATATITLMNVPAPTNNHKH"
     CDS             complement(5881..6258)
                     /locus_tag="ctg35_7"
                     /transl_table=11
                     /translation="MTNDINFWTGIKDPYLKPDKPFLTEDKELKIIHLIQSYPMHCPLC
                     GQLMRRNGFRKRPVTIKILTPAGKPAVLKIKKQQYLCPPSPQCPKRVTKVAEIQGVNFA
                     CRIANVVKYHIVQELSESVRN"
     CDS             complement(6471..7229)
                     /locus_tag="ctg35_8"
                     /transl_table=11
                     /translation="MMYEFPRLTAHHIQTTDQPVVTSTNEMAKERLKVKDQPFLISARQ
                     QTAGHGKTSRKFYSPAGSGAYFTLALPFSYLAKSAGQVTVTAAVAAYEVLSKQFTVPIT
                     IKWVNDLYCERKKVVGILAEMEMTANNQPRGIVVGWGINLAAPTAPLPPALQLRAGWLG
                     TTSIDDHQRLQIVDAVAAHFLDLLANASWKHILQVYQAHQYLINKLLLVDTGAERVRGY
                     FDHITDDGWLCVQTDNGPRAFMTGTVRELV"
     CDS             complement(7244..10681)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090:acetyl-CoA carboxylase biotin carboxylase (Score:
                     92; E-value: 5.7e-28)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_9"
                     /sec_met_domain="ATP-grasp (E-value: 9.1e-12, bitscore:
                     34.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 2.2e-11,
                     bitscore: 33.3, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="HMGL-like (E-value: 1.2e-36, bitscore:
                     116.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKKVLVANRGEIATRIIRACHELGIKAVAVYAKEDEYSAHRFAA
                     DEAYQIGAGEHPLDAYLDIDEVIRVAKESGAEAVHPGYGFLAENADLAQACIDNGLKFI
                     GPKPEHLRMFGDKLVAKRVAVEAGLQPIPGLSGDVKNVDQVKDFAHQYGYPIMVKAANG
                     GGGRGMRIINDDQQLTDEFDQAKDEALKAFGSDEMYVEKDVQHPKHIEVQVIADEHGHV
                     MHLFERDCSVQRRHQKVIEFALSVVLTPERRVEVCGLAVKLMKSVHYQNAATIEFLATP
                     DHFYFIEVNPRVQVEHTVTELITGIDIVKTQIRVAAGEDLHQDIGLPQQDDLKFHGAAI
                     QCRVTTEDPENNFMPDTGQVTTYDAPGGTGVRLDGNVYPGYKVTPYFDSLLVKCCTRGE
                     DFSDARIKMLRALEEFKIRGVKTNIPFMINVLKHPTFIAGKCSTTFIDENPDLFSFAHR
                     PDTPNQLLQYIGNVTVNGFAGVTKRTKLVLPDMQEPQAGQLTPNQHTTKTAKDILDKQG
                     ADAAMQYVKDQPRVLLTDTTMRDAHQSLFATRMRTHDMLPAMKVYDRAMPNLFSVESWG
                     GATFDVCYRFLGEDPWTRLRLLKKYMPHTLQQMLLRASNAVGYQNYPDNELKRFIEHSA
                     ATGVDVFRIFDSLNWIDQMERSITYVRETGKIAEGTMCFTGDFLSPHEHKYTLDYYMKL
                     AQSLVDAGAQIIGIKDMAGLLKPKAAYELVGSLKAKFDVPVHLHTHDTTGNGVPTYVAA
                     TNAGVDVVDVAMSSMAGTTSQPSLATFYYALEGDKRQPDLNMHNVAVLNRYWSNVRPLY
                     NDFSNGVNAPQPDLYQTEMPGGQYSNLKQQAQSLGINDFSLVEEKYREVNQLLGDIIKV
                     TPSSKVVGDMAIFMIQNHLDKDNILTKGQRVDFPESVVNFFAGDLGQPYRGFPKDLQKV
                     VLKDHPAITVRPGSLAKPVDFAAVKQELAAKINQEPSEDDLQGYIMYPKVFTTYFRNHQ
                     RYGEVMDLDTTTYYQGMRPGEILHVKYGQGKEMIIKLDFVSAPDDDGDRFLFFELDGRI
                     LKIKVKDNSMKDKVVSVSKADPDKSNEIGMPLNGNVVKVNVAEGDQVKVGDVLVVTEAM
                     KMESAVKAPFTGKVTKVYAKVGDALKSQDLLLTLTEDA"
     CDS             11382..12326
                     /locus_tag="ctg35_10"
                     /transl_table=11
                     /translation="MTRKVAVVGMGHVGATVAHYLVAGGFTDDLALYDTNEAKVRADAL
                     DLRDAMANLPFHTNLTVNDDSQLADCDVVISTLGKSKLVDTPDHDRFAEFKFTRTQVPL
                     VAKKLVDNGFHGKLVDVTNPCDVITSMYQKLTGLPKNHVMGTGTLLDSARMRACVGEAL
                     HVDSRSVVGFNLGEHGNSQFTAWSTVRVLGKRVLDLVDERHLDLTDMEERARQGGYMVY
                     KGKKYTNYGVATAAVRLTNALLSDARTEMPVSNYREEYGTYLSYPAVVGRDGVVEQLHL
                     DLTAAEKEKLATSAKYIKDRLSAEEQRAAQQDA"
     CDS             12693..12992
                     /locus_tag="ctg35_11"
                     /transl_table=11
                     /translation="MVIDTHTEIYDLLREYERTCVTITFKDGTSKKIFFLDVDDEYQEN
                     DVRPEKDCIVYNITDSISYGNGIPLDKLRSSPKCWCKLNSANLSFHKLKHTGFF"
     CDS             complement(12957..13037)
                     /locus_tag="ctg35_12"
                     /transl_table=11
                     /translation="MLNMTVYHGIEKDDNSEEAGMLKLVK"
ORIGIN
        1 tgccgtgtaa gacgaaacgg ttcgtagaac tgttcgcagt ggtgtatcta tgcaaattgt
       61 gattcaaaca ctcatcagaa gggtgcttgc tcacaagcta ctgactttag tcagtggtgg
      121 ttgactttgc gtatgttaga tagaaaacgt ggtcaataac cacaggccta gtgtctaagc
      181 cggccgaaga acgatgcgtt ccgcccctag cttagtcata cgacctgcca agcaagttat
      241 ttgccactcc cttttaatta gtcgatatcg ttgcaaatta cgaccggctt gattaagtcc
      301 gccgacttgt ggtgcatcag ttcgatggcg tccggaatct tctcgaagcc ttcgaagcga
      361 tgggtgatca ttagtgatgg atcgatccgg ccgttcacca cgaggacggc cagcttttcc
      421 atccgcaggc ggccaccagg catcaggcca ccgtggatgg tcttgtgacc catcccaaca
      481 ccccagtcac cggcgttgaa gaccacgtcg tcgtgaccgt ttaagtagtt aacgttcccg
      541 attgagccac ccggcttgag catccggacg gcttcttcaa aggtgtcctt ggcgttccca
      601 ccggcgatca ggaccttgtc gaccccttgg ccaccagtgg cttccaggat ttggtcggaa
      661 atccggccgt tgtggtagtc aatgatgtcg gttgcgccgt actttttagc cacttcgacc
      721 gtcttttccc gtgaaccaac cgcaaagacg cggccagccc cgtggagcac cgccccgcgc
      781 agggccatca agccaaccgg gccgatccca atcacggcga cgtcatcccc aaaggtcacg
      841 cccgccattt cggaagcgtg gaggccagtt gggatcatgt cggaaagcat gcaggcctcg
      901 gccgggtcaa tttcatccgg taggtgagcc aggttcccgt cggcgtcgtt aacgtggaaa
      961 cgagtggcga agaccccgtc cttgatatta gagaacttcc agccgcccag catcccgtcg
     1021 gaatcagtgg cgtagccagc ttgggagtaa acgtcagacc agtttggcgt gatcgccgga
     1081 acgagtaccc ggtcacccgg tttaaagtcc ttgacgtctt caccgacttc cacaaccacc
     1141 ccgcaggctt cgtggccgag aatcatattc tcccgtggcc ctaggcatct tcaaacacgg
     1201 tgtggatgtc agaagtacac ggggcaacgg ctaggggacg taccagggcg tcacggctgc
     1261 ccaccttggg atcttctttt tcaataaagc ccgtcttgtt ggggcccagc attgcaaaac
     1321 ctttcattca taactaccac ctttcttgat tagttgccgt cattctaaca taaatcatgt
     1381 tataaccaag tcttcatatg tgagtttaat taattttaaa gaaagcgtcc acattctctg
     1441 aaagggttaa aataacaggg attatgatag ttaagaaatt gatttgaaat aaataaaatg
     1501 actgtgataa tcccgtacga ggtcaccaca gtcattaatc attctataat tagcggatta
     1561 tttagctttt aaattcccaa tgaagaggga cgccaccccg gccaccaagg cggagatgac
     1621 caggccgaag aaggccgtgt cgtatgaacc gctcaactga acgatccagc cgattaagag
     1681 gggaccgaag aagcccccga gttggccacc aaagttaatc gtcccgatct gggagccata
     1741 agtcttttgc gggaagagtt gggccgcgta ggagaagatc ccggtgaagg ctagcgactt
     1801 gacgaagtag gtggccattt ggaagatcac taaccaaacc aggctggtgg acttgtagag
     1861 tccaaacatg gtcaggaccg ttaaaaagga ggccaggata atcatcaggc gttcgtgatt
     1921 tttaaagatg tgcaccatta cgtaaccgga aatcaaggcc gcaatccccc cggcaatcgt
     1981 cggtagggga actaaccaag ccaagttggc taagttgatg tggcgggcgt ttaagaagta
     2041 aaccggcatc caggtttcca tccccttggt gataatgttt aaggccagcc cgatcaccac
     2101 gaaggcccag acccgcgggt cgatttcctt ccagtttatc ttttgccgtt cttgtttcgt
     2161 taccgtttgt ccaccgccgt gaccgcggaa gaagaggtag ctgaccatca agagtaggcc
     2221 aaagcccccc aaccagaaga aggccgagcg ccaaccggta gcggcgatga ttggcacgat
     2281 gatgaaaggg gccagggccg cgccggcgta gttagaggaa attaaggcgg cggtcagttg
     2341 ggcacgcttg ttgaccggga agtcctgggc gacttgcttc atggcggccg gtgggtaagg
     2401 gccctcggcg atcccaaaca ggaaccgaat taccaagagg gcggctaagg accaggccag
     2461 cccggtaaag atcgtcatca ccgaccaaaa ggcgagggtg gcgatgatca ccttacggga
     2521 agagaagcgg tcggtcagcc agccccccgg gatttgcatg atcgtgtagc ccaagaagaa
     2581 gacggaacta gtcgccccca gtgcggccgg gctaacggag aaatccttgc cgatcgcggc
     2641 caacgataag ttaagcacgg tccggtcggc gaataagagc atgtaaccaa ggtataaaat
     2701 cacaaaggaa ccgagcgaca gcgcccggcg gttgggtttt gcatccatca tttatttgta
     2761 tctttctttt ctcaattgat tattctgttg tggcatgact aagcgttagc gaccaggtct
     2821 tgtaagcgaa acttttggac cttgccgctg gcgtttttcg gtagggcgtc tgtttgcaag
     2881 tagaccttgg gtaccttata gtgggccagg tgttcacgcc cgaaggcctc caactgctga
     2941 aagtcgacgg gttgtttgct gacgaccacg gccacgggaa cggcgcccca ctgttgatca
     3001 ttacggccga ccacggcaat ttcttggatg cctgggtact ggcgcaagac gttttccacc
     3061 tcggcgggga aaatgttttc gcccccggag atgatcattt catctaaccg tccgtcgacg
     3121 tacaagaaac cgtcttcatc aaagtggccg acgtcgccgg ttcggtacca gtcgccatcg
     3181 tgcttagccg caaacttggc cggttggttt aagtaacccg gggttaaggc cggggtctgg
     3241 atctcgattt cgtgatgctc acccaccaaa cgcagctggg tggtaaacag gggcttaccg
     3301 accgaaccaa ggtggtaagg cgcctcttca gccgagaggg caacgaactg cgagcaagtt
     3361 tcggtcatcc cgtaacactg gacaatcggc agcccacttt cttcgcaggc gttaagggtc
     3421 gcccggtcaa tcgtcccacc gccgagtagc accaggcgaa aggccgatga gtaacagtgg
     3481 tcatttgctt ggacgtcttc gagcatcttc tttagcatgt agggaacggc cgaaatgatc
     3541 gtaaccggtt cgtcctccag gatttggttg accacttgcg catcgaaatg gttgactagg
     3601 cgaacgccca tcccgtaaat caggccccgc attagaattg aaaagcccga aatgtggaag
     3661 atgggggcga cgcacaacca gaggtcgttt ggcaacaccc cgaggttcaa agatgacgaa
     3721 atggcggagg ccaagtggtt gccgtaggtt tgcaagacgc cctttggctg accggtggtt
     3781 cccgaggtgt acatgatcga ggccacggcg tctaggtcaa ccgtcggcgt caacactgcc
     3841 gccggggcag tgccggctgt caattccgaa cagaggaccc ggtgcccacc gtaatagcgg
     3901 tcggctagtt ggtcatcgac taagactagg tcgagccccg cgtcgccgat ttggcggctc
     3961 agttcttcag gggcaaggcg ccaatttagc caaacgatgg tcttgtcggc ggctaagacc
     4021 cccagggcgg ctaagtaacc agccaggtta ttgtgggtca tcaccccaac ccggggctgg
     4081 tccgtctgcg cttgaatctg gccagcacgg tgggcgacca ggggaaccag ctgggcaaag
     4141 gtgtaggcgg tggtaccgtc gttaaccgcc acccggttag ggtttagcga agcctgtttg
     4201 aggagccaat ttggaatctc catgacggcc tcctacggga acttcgggaa cttatcgaag
     4261 tcggggtcgc gcttttggtt aaaggcgtcg cgcccttcct ttccttcgtc agtcgtgtag
     4321 taaagcatcg tggcgtcacc ggcaaattgt tgaagccccg ctaaaccatc tgtatcggcg
     4381 ttcatggccg ccttaatgaa gcgaagggcg gtcggtgact tctttaagat ttcatgacac
     4441 cagtctaaag taactgattc gacctcagct aggggcgcca ccttgttaat ccagttcatt
     4501 tggtaagctt cgtcagccgt gtagaagtgg tttaagaacc aaacttcctt ggcccgcttg
     4561 tggccgatca cccgggctag gtaacccgag ccataaccgg cgtcaaagga acccaccttc
     4621 ggccccgttt gcccaaacat ggcgttatcg gcggcgatcg tcaggtcaca aaccagttgt
     4681 aagacgttcc cgcccccgac tgaccagccc ttgaccatgg caatcaccgg cttggggatg
     4741 atccgaatca agtgttggag gtccaaaacg ttgagccggg cgatgtggtc ggggcccacg
     4801 taacccccgt taccccgcac ggattggtca ccaccggaag aaaaggcctt atcgccggct
     4861 ccggtaagaa taatcacgcc aattgtgcta tcatcacgac agatgttaaa cgcgttgatc
     4921 atttcggcga ccgttaccgg ggtgaaggca ttgtgctttt ccggccggtt cattgtaatc
     4981 ttggcaatct tatcgtcgcg ttcaaaaata atttcggaat actccttaac gggggtccag
     5041 ttagcactcg tcataacaaa ctcctttaca aaatccagag gtacaaaaat gaatttactt
     5101 gaaaacttag gcattcacca ggacttggtg accaaagacc gcgttgaact ctccatgccg
     5161 gtccaagcaa agcacttgca accgtacggg atcgttcacg gcggaattaa caccgtgctg
     5221 gcggaaaccg cggcctccct aggggctaac gccaacgtag cgccggggcg aatcgcggtc
     5281 ggggtcaatg ttaccacaca acatatccgt gcgatacggg aaggggagat ttttgtggtt
     5341 gcaacccctg atcacgttgg tcgcacactt cagacctggc aagcccggct actggttaac
     5401 gaccgattaa cggctaccgc aaccatcacc ctgatgaacg tcccagcacc aaccaacaat
     5461 cataaacatt aattcacaaa ttggctacta ttatgcttag aaagttgccg ggtgaccatt
     5521 gggggattac ctaaatcaca ttttttaatt ttatattatg taaatcaatc gagaagtcgt
     5581 cggctaactg ttatgaaagg ggcgccacta cgcagtcgat ttaagttggc cccgattttg
     5641 tattagtgtt gcaaccagta cgcaaaagca cacataattt atcgagggga ggaccagata
     5701 tgaggaaatg gtgggtggaa ttgaaactgg tcgtcttttg catcgccgct cttttcaggt
     5761 aattactcta acggcgctaa ttagacttac ttctgtaaat ttacattatg taaagatatc
     5821 gtttgatgat taaagaagcc tcaaaggccc tccgaggatg acaatacaga tgttcaagac
     5881 ttaattacgt acgctctcac tgagttcctg aacaatatgg tatttaacga cattcgcaat
     5941 tcgacaggca aaattaactc cttgaatctc ggcaaccttg gtaactcgct tggggcattg
     6001 gggcgatggt ggacagagat actgttgttt cttgattttt aagacagctg gttttccagc
     6061 cggtgtaagg attttgatcg ttacgggccg cttacggaag ccattacgac gcatcaactg
     6121 gccgcaaagc ggacagtgca tgggataaga ctgaattaaa tggataatct ttagttcttt
     6181 atcttcagtt aaaaatggtt tgtcaggttt taagtaagga tccttgattc cagtccaaaa
     6241 attgatatca ttagtcatga ggaatatatc ctttctaaga caactgagtg gttatgggga
     6301 taacttaaga cagttgtctt tttttatttt taaatcaatg gtacagcaag taacaaaaaa
     6361 cggtaaagac aagaaatcat cttatcctta ccgaatatca tagaaccttc aaggataact
     6421 gcggtcaaaa cacgtccgcg tttgtccaag aaggactgct tttttaatta ctaaaccagt
     6481 tcccgaaccg tccccgtcat gaatgcgcgg ggcccgttat ctgtctgcac acataaccag
     6541 ccatcatccg taatatggtc aaagtatccc ctaacacgtt ccgctcctgt atcaacgagg
     6601 agcagcttgt taataaggta ttgatgagcc tggtaaacct gcaggatgtg tttccaggac
     6661 gcgtttgcta acaggtcaag gaagtgcgca gcgactgcat caacgatttg tagccgttga
     6721 tggtcatcaa ttgaggtggt gccgagccat cccgcacgta actgaagtgc tggcggcaag
     6781 ggagcagttg gtgccgccag gttaatcccc cagccgacca ctattccccg cggctgatta
     6841 ttcgcggtca tttccatttc agcaagaatc cccaccacct tctttcgctc acagtacagg
     6901 tcgttcaccc atttaatagt aatgggaaca gtgaattgct tgctgaggac ttcataagca
     6961 gcgaccgcgg cagtcaccgt tacctgacca gcactttttg ccaggtagct aaagggtaag
     7021 gcaagggtga agtaggcccc acttcccgcg ggtgaataaa acttccggct ggtctttccg
     7081 tggcctgccg tttgctgccg ggcggagatg agaaagggct gatctttcac ctttaaccgt
     7141 tcctttgcca tttcgttagt cgaagtgacc actggctgat cggttgtctg gatatgatgt
     7201 gctgtcagtc taggaaattc gtacatcatt gctactctcc ttatcaagca tcctcagtca
     7261 atgttagtaa gaggtcctga ctctttaacg catcgccaac cttagcgtat actttcgtca
     7321 ccttaccagt aaatggcgct ttaactgctg attccatctt catcgcctca gtaacaacca
     7381 gaacgtcacc aaccttgacc tggtcacctt ctgctacgtt aaccttgacc acgttcccgt
     7441 tcaacggcat cccgatctca ttactcttat cgggatcagc ctttgaaaca ctgacaacct
     7501 tgtccttcat gctattgtcc ttaactttta ttttaaggat ccgcccgtcc agttcaaaga
     7561 agaggaagcg atcaccgtca tcatctggtg ctgaaacaaa gtcgagtttt ataatcattt
     7621 cttttccctg accatactta acatgcaaaa tttcaccagg acgcatacct tgatagtagg
     7681 tcgtggtatc aaggtccatc acttcaccgt accgctgatg gttgcggaag taagtggtaa
     7741 agactttcgg atacatgatg taaccctgca ggtcgtcctc gctcggttcc tggttgatct
     7801 ttgcggccag ttcctgctta acggcggcaa aatcaaccgg tttagcaagg ctcccgggcc
     7861 gaaccgtgat tgcaggatgg tccttcaaga caaccttctg taggtccttg gggaagcccc
     7921 tatatggttg accaaggtcg ccagcaaaga agttaaccac tgattctgga aagtcgactc
     7981 gttgcccctt tgtcaggata ttgtctttat caaggtggtt ttggatcatg aagattgcca
     8041 tgtcgccaac caccttcgag cttggcgtca ccttgatgat gtcaccgagc aactgattga
     8101 cctcccggta tttttcttcc accaggctaa aatcattgat tcctagtgac tgggcctgtt
     8161 gcttcaggtt cgagtactgg ccacctggca tttccgtctg gtataggtct ggttgcggtg
     8221 cgttgacccc gttgctgaag tcgttgtaaa gcggccggac attggaccag taccggttta
     8281 agaccgccac gttatgcata ttaaggtccg gttggcgttt atctccttct agcgcgtagt
     8341 aaaaggtggc taaactaggc tgactggttg tccccgccat tgaagacatc gccacgtcca
     8401 ccacgtcaac acccgcattt gtggcggcaa cgtaggttgg aactccatta cccgttgtgt
     8461 catgagtgtg gaggtgaaca ggaacatcaa acttagcctt caaactaccg actagttcgt
     8521 aagcggcctt tggtttcaag aggccggcca tatccttaat accaataatc tgcgctcccg
     8581 cgtcgactaa tgactgtgcc aacttcatgt aataatctag ggtgtacttg tgctcgtgag
     8641 gactaaggaa gtcgccggta aagcacattg ttccttcggc gattttgccg gtttcccgaa
     8701 catatgtaat gctacgttcc atctggtcaa tccagttgag ggagtcaaag atccggaaaa
     8761 catccacccc ggttgcggca ctgtgctcaa tgaaccgctt cagttcatta tccggataat
     8821 tctggtaacc gaccgcgttc gacgcccgca acaacatctg ctggagagta tgtggcatgt
     8881 acttcttcaa taatcgtaat cgggtccacg ggtcttcacc aaggaaccgg tagcacacgt
     8941 caaaggttgc cccgccccag ctttcaacag aaaagaggtt aggcatcgcc cggtcgtaaa
     9001 ccttcattgc gggcaacata tcgtgcgtcc gcatccgagt ggcaaaaagg ctttggtgag
     9061 catcacgcat tgtggtatca gtcagtagta ccctcggctg gtccttaaca tactgcatcg
     9121 ccgcatcggc tccctgttta tccaggatgt cctttgctgt ttttgttgta tgctggttag
     9181 gtgtaagttg gcctgcttgt ggttcttgca tgtcaggcaa aacaagtttg gtccgcttgg
     9241 ttacccctgc aaagccattg acggtaacat tgccaatata ttggagcaac tggttaggcg
     9301 tatcgggccg gtgcgcaaag ctaaagaggt cgggattctc gtcgataaac gttgtcgagc
     9361 attttccggc aataaacgtg ggatgcttta acacgttgat catgaaggga atattagttt
     9421 tgactccccg aatcttgaat tcttcaagcg cccgcagcat cttaatccgg gcatcactga
     9481 agtcttcacc ccgggtacaa cacttaacga gcaaggaatc aaagtaagga gtgaccttat
     9541 agccgggata gacgttcccg tcaagccgga caccggttcc ccctggcgca tcataggtcg
     9601 taacctgtcc cgtatctggc ataaagttat tctcgggatc ttctgttgtg acccggcact
     9661 gaatggctgc cccgtggaac ttgagatcgt cttgctgggg aaggccaata tcttgatgaa
     9721 gatcttcacc ggccgcaacc cgaatctggg tcttaacaat atcaatccca gtaatcaact
     9781 cggtaacggt gtgttcaacc tgcacccggg gattaacctc aatgaagtaa aaatgatccg
     9841 gggtcgccaa gaattcgatc gtcgccgcat tctggtagtg aacactcttc atcagcttga
     9901 cggccaggcc acaaacttcg acccgccgct caggggtcaa gacaacgcta agtgcaaatt
     9961 caataacctt ctggtggcgg cgctggacag agcagtcacg ttcaaagagg tgcatcacat
    10021 gcccgtgctc atcagcaatt acctgcactt caatatgctt cgggtgttgg acgtcctttt
    10081 cgacatacat ctcatccgat ccaaacgcct tcagcgcctc atccttcgcc tgatcaaact
    10141 cgtcggtgag ttgctggtcg tcattgatga tccgcatccc ccgaccgcca ccaccattag
    10201 cagccttaac catgatcggg taaccatatt ggtgagcaaa atcctttacc tggtcaacat
    10261 ttttaacatc ccccgataaa ccgggaattg gttgcagccc tgcctcaacg gccacacgtt
    10321 tagcaaccaa cttatcccca aacatacgaa ggtgttccgg cttaggtcca ataaacttta
    10381 atccattatc aatacaggcc tgcgcaaggt cagcgttttc tgccaggaag ccataaccag
    10441 ggtgaacagc ttctgccccg ctttctttgg ccacccgaat tacttcatca atatccaggt
    10501 aagcatcaag cggatgttca ccagccccga tttggtacgc ctcatcagca gcaaagcggt
    10561 gcgcactata ctcatcctcc ttagcataaa ctgcgacggc tttgattccc agctcatgac
    10621 aggcgcgaat aatccgtgta gcaatttcac cacggttagc aactaaaact tttttaaaca
    10681 caattatcca ccatcctttt tattaatcct tcacgcccat cacgtcatta ctattggcgg
    10741 tgagggtggc tgtatttgta gaaatattaa tattaccaac cattgcactc acctcctcat
    10801 ttttctaatc aaattttaaa acaaactata atcaatctaa gctaacgtgt caataatttt
    10861 tcctcaagaa atgcccgcta tcaaacgaat ttatactata aattatctgc ctttgcaccc
    10921 atcttttctc atcttttttt aatttttatc ttgacttccc tatctgccta gtgatatagt
    10981 acttaacaat caattgaaac aagttaaaga cgttgagcga gaaacagcga tgaggccgaa
    11041 gcaagagagc tggtgggtgg tgtgaaccag tgagaggccg cacctactaa tcactcgtga
    11101 gtcgtacgcc gaacggattc cctagtagag cgtattggta gcacccatta ccgtgccggc
    11161 taataccaca ttatttgtgg cggttggttg aggcgacatt atgtcgtaaa gaaaggtggt
    11221 accacgcaca agcgtccttt ttagttctta gatttatcaa gagctagaaa ggacgctttt
    11281 tgttttgata ttaaaggtgg tggtgatcat ggcagacagt gatatttctg acattagtaa
    11341 gattaaaggc aacaatacga taatcgaaag gaagttttat tatgacaagg aaagtagcag
    11401 tagttggaat ggggcacgtt ggcgcaactg tcgcccatta tctcgttgct ggtggattca
    11461 ctgatgattt agccctctat gatactaacg aagcaaaggt ccgtgccgat gcgcttgacc
    11521 tccgtgacgc catggcgaat ctcccgttcc acacaaactt aaccgttaat gatgattcac
    11581 aattagctga ctgtgacgtt gtaatctcta ctctgggaaa gtctaagctg gtggacacac
    11641 ccgatcacga ccgctttgca gaatttaaat ttacccggac tcaggttcca ttggtcgcta
    11701 agaaactcgt tgataacggt tttcatggca aacttgtcga cgttaccaat ccctgtgacg
    11761 tcatcacttc aatgtatcag aagctcacgg ggctaccaaa gaatcacgtg atgggtacgg
    11821 ggaccctgct agactccgca cgaatgcgcg catgtgttgg ggaagccctc catgttgact
    11881 cacgatccgt ggtcggcttc aatcttggtg aacacggtaa ctcccaattt actgcctggt
    11941 caacagtccg ggttctcggc aaacgagtac tagatctggt ggatgaacgg cacctcgacc
    12001 ttaccgacat ggaagaacgc gcacggcaag gaggctacat ggtctataag ggaaagaagt
    12061 ataccaacta cggtgttgct actgccgcag tccggcttac caatgcccta ctaagtgatg
    12121 cccggactga aatgccagtt tcaaattacc gtgaagaata cggaacgtac ctctcctacc
    12181 ccgcagtcgt tggtcgtgac ggggtggtgg aacaacttca cctggacctt accgctgctg
    12241 aaaaagaaaa gctcgctacc tctgccaaat acatcaagga tcgcctttct gcagaagaac
    12301 aacgggcagc tcaacaagac gcctaacttg ctggtattaa atgatctctc ctcaaacata
    12361 tccctaataa agcaccaacc ttattctgta taactgaaac ttgaaagctg ataaccatgt
    12421 caaacaggcg aggttatcag ctttcaagtt tcagttaatt aatcaacggc ggagccagct
    12481 taaatagctg gcttttatta tgcagaaagg aggtaaaaat agctaaagtt caaaacgaaa
    12541 tggcgacccg gatcactgtt gatactgtcc aggcggctaa ttccatgcag gcaatgcgtc
    12601 aggctatctc agccaatacg aatgcatgga aggcaattaa aaatcaacca ttctgtgaca
    12661 gaatattaag tagtactggg gagtgaataa agatggtgat cgacacacat actgaaatat
    12721 atgacctcct aagggaatat gaacggactt gtgtgaccat tacgtttaaa gacggaacgt
    12781 ccaagaagat tttctttctg gatgttgacg atgaatacca agaaaatgat gtgagacctg
    12841 aaaaagattg tatcgtatac aatataacgg attcgatatc atatggtaat ggcatcccgc
    12901 tagataaact taggtcaagt ccaaaatgtt ggtgtaaatt aaattccgcg aatttatcat
    12961 ttcacaagct taagcatacc ggcttcttct gaattgtcat ctttttcaat cccgtggtaa
    13021 acggtcatgt ttaagtac
//
