GCA_027857955.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaKIOLHBIL_1 (original name was: gnl|Prokka|KIOLHBIL_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	NRPS-like	51,983	94,874	asukamycin	Polyketide:Type II polyketide	4%

gnlProkkaKIOLHBIL_2 (original name was: gnl|Prokka|KIOLHBIL_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	lanthipeptide-class-iv	1	19,361

gnlProkkaKIOLHBIL_15 (original name was: gnl|Prokka|KIOLHBIL_15)

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	NRPS-like	1	12,400

gnlProkkaKIOLHBIL_23 (original name was: gnl|Prokka|KIOLHBIL_23)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	RaS-RiPP,lanthipeptide-class-iv	127,321	145,806

gnlProkkaKIOLHBIL_34 (original name was: gnl|Prokka|KIOLHBIL_34)

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	RaS-RiPP	82,056	104,161

Compact view

gnlProkkaKIOLHBIL_1 - Region 1 - NRPS-like

Download region GenBank file

Download region SVG

Location: 51,983 - 94,874 nt. (total: 42,892 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

NRPS/PKS substrates

NRPS/PKS substrate predictions

ctg1_78

Search NORINE for peptide: strict or relaxed

AMP-binding (6..388): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVTSCALIYK
Nearest Stachelhaus code(s):: Ala DVTNFALIYK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaKIOLHBIL_2 - Region 1 - lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 1 - 19,361 nt. (total: 19,361 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg2_8

ctg2_6

Leader:

MSEEILMLQSMSVEEEINEVN

Core:

GQRFSTMSNYHCGGANFSLASNHYCRRK

Core with dehydrations:

GQRFDhaDhbMDhaNYHCysGGANFDhaLADhaNHYCysRRK

antiSMASH-DB 4.0 matches for ctg2_6

100.0% NZ_JADNKH010000035.1and 1 other:

NZ_OU540596.1 Lanthipeptides: JQ838_RS08065_lanthipeptide

Lanthipeptides: I2E19_RS11270_lanthipeptide

1

GQRFSTMSNYHCGGANFSLASNHYCRRK

28

GQRFSTMSNYHCGGANFSLASNHYCRRK

1

GQRFSTMSNYHCGGANFSLASNHYCRRK

28

42.9% NC_012470.1and 5 others:

NZ_CABY01000005.1 Lanthipeptides: SZBHS5_RS10335_lanthipeptideNZ_CP065058.1 Lanthipeptides: I2434_RS07250_lanthipeptideNZ_CP078012.1 Lanthipeptides: KV238_RS00510_lanthipeptideNZ_LS483328.1 Lanthipeptides: DQL47_RS07600_lanthipeptideNZ_LS483368.1 Lanthipeptides: DQM56_RS07295_lanthipeptide

Lanthipeptides: SZO_RS10195_lanthipeptide

1

GQRFSTMSNYHCGGANFSLASNHYC

25

G RFST S CG N+S S C

1

GMRFSTTSLSWCG--NWSAISAFGC

23

39.3% NZ_KB946328.1: Lanthipeptides: UC3_RS18350_lanthipeptide

1

GQRFSTMSNYHCGGANFSLASNHYC

25

G RFS++S CG N S S C

1

GARFSSLSGGSCG--NLSSVSIWDC

23

with 4 more sequence matches

35.7% NZ_MSKU01000037.1: Lanthipeptides: allorf_43371_43508_lanthipeptide

4

FSTMSNYHCGGANFSLASNHYC

25

ST+SN HC G S + YC

1

ISTISNEHCWGWP-SAVTTRYC

21

32.1% NZ_CP084108.1and 2 others:

NZ_CP102383.1 Lanthipeptides: NP781_RS20720_lanthipeptideNZ_JAASJR010000006.1 Lanthipeptides: HCH28_RS14710_lanthipeptide

Lanthipeptides: LCP48_RS21615_lanthipeptide

4

FSTMSNYHCGGANFSLASNHYCRR

27

+ST SN+ C G S+ S C +

2

YSTTSNFLCIGTP-SMMSQFTCSK

24

21.4% NZ_JACHIN010000025.1: Lassopeptides: allorf_47202_47381_lassopeptide

12

CGGANFSLASNHYCR

26

CGG ++ +YCR

3

CGGPHWEWIYPNYCR

17

14.3% NZ_LR134504.1and 1 other:

NZ_LR134504.1 Lanthipeptides: allorf_1623800_1623958_lanthipeptide

Lanthipeptides: allorf_1623800_1623958_lanthipeptide

1

GQRFSTMSNYHC

12

G RF T + Y C

1

GSRFETTAQYSC

12

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg2_6 - Class III

Cleavage pHMM score: 1.30

RODEO score: 0

Monoisotopic mass: 3101.4 Da

Molecular weight: 3103.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3121.4 Da N = 1; 3139.5 Da N = 2; 3157.5 Da N = 3

gnlProkkaKIOLHBIL_15 - Region 1 - NRPS-like

Download region GenBank file

Download region SVG

Location: 1 - 12,400 nt. (total: 12,400 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 12400:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg15_5

Search NORINE for peptide: strict or relaxed

AMP-binding (9..397): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVTAVAAVYK
Nearest Stachelhaus code(s):: Ala DVFSVAAVYK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaKIOLHBIL_23 - Region 1 - RaS-RiPP,lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 127,321 - 145,806 nt. (total: 18,486 nt) Show pHMM detection rules used

Region on contig edge.

RaS-RiPP: (PF04055 and TIGR01716)
lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

gnlProkkaKIOLHBIL_34 - Region 1 - RaS-RiPP

Download region GenBank file

Download region SVG

Location: 82,056 - 104,161 nt. (total: 22,106 nt) Show pHMM detection rules used

RaS-RiPP: (PF04055 and TIGR01716)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.