GCA_028310695.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaANCPJANK_8 (original name was: gnl|Prokka|ANCPJANK_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	RRE-containing	2,026	23,570

gnlProkkaANCPJANK_22 (original name was: gnl|Prokka|ANCPJANK_22)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	NRPS	1	36,130	dipeptide aldehydes	NRP	47%

gnlProkkaANCPJANK_27 (original name was: gnl|Prokka|ANCPJANK_27)

Region	Type	From	To	Most similar known cluster		Similarity
Region 27.1	cyclic-lactone-autoinducer	1	15,786

gnlProkkaANCPJANK_29 (original name was: gnl|Prokka|ANCPJANK_29)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	lanthipeptide-class-ii	1	16,712

gnlProkkaANCPJANK_34 (original name was: gnl|Prokka|ANCPJANK_34)

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	ranthipeptide	172,466	194,054

gnlProkkaANCPJANK_51 (original name was: gnl|Prokka|ANCPJANK_51)

Region	Type	From	To	Most similar known cluster		Similarity
Region 51.1	lanthipeptide-class-ii	1	10,441

Region	Type	From	To	Most similar known cluster	Similarity
Region 8.1	RRE-containing	2,026	23,570
Region 22.1	NRPS	1	36,130	dipeptide aldehydes	NRP	47%
Region 27.1	cyclic-lactone-autoinducer	1	15,786
Region 29.1	lanthipeptide-class-ii	1	16,712
Region 34.1	ranthipeptide	172,466	194,054
Region 51.1	lanthipeptide-class-ii	1	10,441

Compact view

gnlProkkaANCPJANK_8 - Region 1 - RRE-containing

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Location: 2,026 - 23,570 nt. (total: 21,545 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaANCPJANK_22 - Region 1 - NRPS

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Location: 1 - 36,130 nt. (total: 36,130 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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transport-related genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 36130:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg22_9

AMP-binding (22..421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1035..1423): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2029..2420): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaANCPJANK_27 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 15,786 nt. (total: 15,786 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaANCPJANK_29 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 16,712 nt. (total: 16,712 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg29_7

ctg29_6

Leader:

MYKEVIRREELMEVIGN

Core:

VEKEKKILLEIQNKNTEQKITQELTGAFLTILCC

Core with dehydrations:

VEKEKKILLEIQNKNDhbEQKIDhbQELDhbGAFLDhbILCysCys

MIBiG 3.1 matches for ctg29_6

11.8% BGC0000553 (SSGG_RS09925): Lanthipeptide: SRO15-2212

25

TGAFLTILCC

34

TG+ +++L C

1

TGSQVSLLVC

10

antiSMASH-DB 4.0 matches for ctg29_6

100.0% NZ_JADPCB010000035.1: Lanthipeptides: I4W51_RS14990_lanthipeptide

1

VEKEKKILLEIQNKNTEQKITQELTGAFLTILCC

34

VEKEKKILLEIQNKNTEQKITQELTGAFLTILCC

1

VEKEKKILLEIQNKNTEQKITQELTGAFLTILCC

34

23.5% NZ_CP048626.1: Lanthipeptides: GXM18_RS04600_lanthipeptide

14

KNTEQKITQELTGAFLTILCC

34

NTE +F TI CC

4

NNTEDIYISIDNASFFTIFCC

24

23.5% NZ_MCGI01000004.1and 1 other:

NZ_MPDJ01000004.1 Lanthipeptides: BLA28_RS33805_lanthipeptide

Lanthipeptides: BEH84_RS33640_lanthipeptide

6

KILLEIQNKNTEQKITQELTG-----AFLTILCC

34

+L E++ E + ++ FL+I CC

1

NVLFEVEESGKEMMNSNQIPANTESTPFLSIFCC

34

with 10 more sequence matches

21.1% NZ_CYZG01000001.1: Lanthipeptides: ARA00_RS15445_lanthipeptide

26

GAFLTILCC

34

GAFLTI CC

30

GAFLTIYCC

38

20.6% NZ_CP036170.1: Lanthipeptides: HDCHBGLK_RS18795_lanthipeptide

26

GAFLTILCC

34

G FLTI CC

11

GTFLTIYCC

19

20.6% NZ_CP070036.1and 2 others:

NZ_JADMPO010000043.1 Lanthipeptides: I2I27_RS16805_lanthipeptideNZ_JAQMLK010000037.1 Lanthipeptides: PNW18_RS16660_lanthipeptide

Lanthipeptides: I6K72_RS03970_lanthipeptide

27

AFLTILCC

34

AFLTI CC

19

AFLTIYCC

26

20.6% NZ_CP102267.1and 2 others:

NZ_JADMOE010000006.1 Lanthipeptides: I2G55_RS05425_lanthipeptideNZ_LT635469.1 Lanthipeptides: BQ7352_RS14540_lanthipeptide

Lanthipeptides: NQ550_RS10225_lanthipeptide

25

TGAFLTILCC

34

T +FLTI+CC

3

TSSFLTIVCC

12

20.6% NZ_CP113781.1: Lanthipeptides: GGADHKLB_RS14125_lanthipeptide

28

FLTILCC

34

FLTILCC

6

FLTILCC

12

20.6% NZ_JADNGF010000061.1: Lanthipeptides: I2E06_RS15650_lanthipeptide

26

GAFLTILCC

34

AFLTI CC

18

SAFLTIYCC

26

17.9% NZ_JADNBM010000025.1and 2 others:

NZ_JAQDZW010000013.1 Lanthipeptides: PGT55_RS13345_lanthipeptideNZ_QVMC01000003.1 Lanthipeptides: DWV46_RS15805_lanthipeptide

Lanthipeptides: I2A62_RS14145_lanthipeptide

27

AFLTILCC

34

AFLTI CC

32

AFLTIFCC

39

15.4% NZ_CP094681.1: Lanthipeptides: K5I26_RS05295_lanthipeptide

28

FLTILCC

34

FLTI CC

33

FLTIFCC

39

15.4% NZ_JAOQJV010000004.1: Lanthipeptides: OCV65_RS05205_lanthipeptide

27

AFLTILCC

34

FLTI+CC

32

GFLTIICC

39

13.2% NZ_LT969517.1and 1 other:

NZ_QVLV01000012.1 Lanthipeptides: DXC51_RS29565_lanthipeptide

Lanthipeptides: C1733_RS32195_lanthipeptide

27

AFLTILCC

34

FL+I CC

31

GFLSIFCC

38

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg29_6 - Class II

Cleavage pHMM score: -1.40

RODEO score: 0

Monoisotopic mass: 3846.1 Da

Molecular weight: 3848.5 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3866.5 Da N = 1; 3884.6 Da N = 2

gnlProkkaANCPJANK_34 - Region 1 - ranthipeptide

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Location: 172,466 - 194,054 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaANCPJANK_51 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 10,441 nt. (total: 10,441 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg51_10

ctg51_9

Leader:

MNEMNDKIIGNVEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg51_9 - Class II: Cleavage pHMM score: -5.90; RODEO score: 0; Monoisotopic mass: 519.2 Da; Molecular weight: 519.7 Da; Number of bridges: 1