LOCUS       c00115_gnlPro..         4917 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PBIKMNCH_115.
ACCESSION   c00115_gnlPro..
VERSION     c00115_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 16:48:02
            Original ID  :: gnl|Prokka|PBIKMNCH_115
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4917
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4917
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[162:2853]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      163..2853
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4917
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4917
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..151
                     /locus_tag="ctg115_1"
                     /transl_table=11
                     /translation="KMNHSNELSNVEKIKQLVDHLNLQHMVPDILMKKQKTLKKLLVFR
                     QINL"
     CDS             163..2094
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg115_2"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-42, bitscore:
                     131.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2e-237, bitscore:
                     777.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPYGGIKMAEQACETYGYKVSDKIKDVFHNYEFKTHNQGVFDIYT
                     PEMKVARHNKILTGLPDTYGRGRIVGDYRRVALYGIDALIEGKQKDFAACDRQGMRRYD
                     FQLREEIADQIRALKGMKVMAESYGYDISKPAKDAREAFQWLYFGYLAAIKTQNGAAMS
                     VGRISTFLDIYIERDLQNGTLTEKEAQELVDHMVMKFRMVKFARIPSYNQLFSGDPVWA
                     TLEVAGMGQDGRSMVTKNDYRFLHTLEDMGPAPEPNLTVLYSSRLPENFKKYAANISVT
                     TSSVQYENDDVMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYAINGGVDEK
                     TKQQVGPNYQPITSEYLDYDEVIAKYDQMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMA
                     LIDTKVERSFATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDFPRYGNDDD
                     RADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDGRKAGEPLA
                     PGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDERSENLVHVL
                     DGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVI
                     ARTCHDRM"
     CDS             2113..2853
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84.4;
                     E-value: 3e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg115_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-29, bitscore:
                     91.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFFSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             3285..4916
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AstE_AspA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg115_4"
                     /sec_met_domain="AstE_AspA (E-value: 6.7e-34, bitscore:
                     105.3, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIINCVHGGWHKVKDLLSEIESYWTTRAEGYSEVNHKELNGMQKG
                     AWLEVLKGQFPEKAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAG
                     NLCERISFYKMDAQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFD
                     ANWYGYLYDEEKRLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDR
                     KFLKENGFESVAVDTGIWQRVWSQEEKLNYHSTPMFMISAVKKEKDIWSENDGTDDSDS
                     RYDRERDLEDAALCTAPGTKKSGFLKLGGGEFSLPYTVICGSHPGKTVLITAAVHGGEY
                     VGIQAAVELADKLKPEKIHGRVILVKTVCRKEFEERSGSICPEDEKNLNRVFPGNPQGT
                     RMDRLAYEVVQKLHSAADYYIDLHSGDDYEQLTPYIYYAGCADEDVVQMSRKMAEQADV
                     PYMVKSNVASGGSYNYAATCGIPSVLIERGQMGGWSPEEVHSTRKDVRNILCALGVYDG
                     MRSYSNYYPMEIEDVRYQSASVSGLWY"
ORIGIN
        1 taaaatgaac cactctaatg aattatcaaa cgtggaaaaa atcaaacaac tggtggacca
       61 tctgaattta cagcatatgg tcccggatat attgatgaag aaacaaaaga ccttgaaaaa
      121 gttgttggtc ttcagacaga taaacctctg aaacgtgctt tcatgccata cggcggtatc
      181 aaaatggcag agcaggcttg tgaaacttac ggatataaag taagtgacaa gattaaagat
      241 gttttccaca actatgaatt taaaactcac aaccagggtg tatttgatat ctacacacct
      301 gagatgaaag tagcacgtca caacaagatc cttacaggtc ttccggatac ttacggacgt
      361 ggacgtatcg ttggcgacta cagacgtgtg gctctgtacg gaatcgacgc tctgatcgaa
      421 ggaaaacaga aagacttcgc agcatgcgac cgtcagggta tgagacgtta tgacttccag
      481 cttcgtgaag agatcgctga tcagatccgt gctctgaaag gcatgaaagt tatggctgaa
      541 tcttatggat atgatatctc caaaccggcc aaagatgcaa gagaagcttt ccagtggctg
      601 tacttcggat atcttgcagc catcaaaact cagaacggtg ctgcaatgag tgtcggacgt
      661 atttccacat tccttgatat ttatattgaa agagatcttc agaacggaac tcttacagag
      721 aaagaagctc aggagcttgt tgaccacatg gtaatgaaat tccgtatggt taaatttgca
      781 cgtatccctt cttacaacca gttattctcc ggtgacccgg tatgggcaac tctggaagta
      841 gcaggtatgg gacaggacgg acgttccatg gtaacaaaga acgactatcg tttcctgcac
      901 acactggaag atatgggacc ggctccggaa ccaaacctta cagtacttta ttcttcaaga
      961 ctgcctgaga acttcaagaa atatgctgca aatatttctg ttacaacaag ttctgttcag
     1021 tatgagaatg atgatgttat gcgtccggta tggggcgatg actacagcat ctgctgctgt
     1081 gtatctgcta cagagacagg taaagagatg cagttcttcg gcgctcgtgc aaaccttgca
     1141 aaatgccttc tttatgcgat caacggcggt gttgatgaga agacaaaaca gcaggtagga
     1201 ccaaactatc agccgatcac ttctgaatat cttgactatg atgaagtaat tgcaaaatat
     1261 gaccagatga tggactggct ggctcatctg tatgtcggaa cactgaacat gatccactac
     1321 atgcatgata aatactacta tgaagctgca gagatggctc tgatcgatac aaaggttgag
     1381 cgttctttcg caacaggtat cgcaggattc tctcatgtag tagactccct gtccgctatc
     1441 aaatatgcaa aagttaaagc tatccgtgat gaagacggta ttacaacaga tttccaggtt
     1501 gagggtgact tcccacgtta tggtaatgat gatgacagag cagacgagat tgcaacaact
     1561 cttctgtcta cattccttga gaaactgaaa cacatccata cttaccgtga ttccaagcca
     1621 accacatcta tccttactat tacttccaac gttgtttatg gtaaggctac aggatctctt
     1681 ccggatggaa gaaaagcagg ggaacctctt gcacctggcg caaacccatc ttacggtgca
     1741 gagcagaacg gtcttcttgc ttccctgaac tctgttgcca agcttgacta tgaagatgct
     1801 cttgacggta tctccaatac tcagacaatc aatcctgatg ctctgggaca cagtgatgac
     1861 gagcgttcag agaatctggt acatgtactt gacggatact ttgatcaggg tgctcatcac
     1921 ctcaatgtaa acgtatttgg taaagagaaa ctgatcgacg ctatggaaca tccggaaaaa
     1981 gaagagtatg caaacttcac aatccgtgta tccggatatg ctgttaagtt cattgacctt
     2041 acaagagagc agcagttaga cgttattgcc agaacctgcc acgacagaat gtaattaatg
     2101 gatgatgata taatggataa taaacagata aaaggctatg ttcattcttt ggaaagcttc
     2161 gggtccgttg atggacccgg agtaagatat gtgatttttt ttagcggctg tgccatgaga
     2221 tgtcagttct gtcataatcc ggatacctgg aagatgaagc agggagagct ttatacggca
     2281 gatgagcttc ttaagaaagc tttacgttat aagggctact ggggcagcaa aggcggtatc
     2341 actgtaagcg gtggtgagcc attgcttcag atggattttc ttacagaatt ttttaagaag
     2401 gcaaaggcag agggtgtgca tacgaatctt gataccagtg gaaatccttt cactgatcag
     2461 gaaccatggc attccggatg gctggagctt atgaagtata cagatcttgt aatgcttgat
     2521 atcaagcaga ttgacgagca ggagcatatt aagcttaccg gtcattccaa taagaatatt
     2581 cttgcgatgg cgcgggaact ttctgatatg aaaaaacctg tatggatccg tcatgtgctt
     2641 gtgccgggcg gaagtgataa ggatgaatat ctgcaccggt tagctgattt tatccatact
     2701 ctttccaatg tggagagagt ggaggtgctc ccttatcata cgcttggtaa gtttaaatgg
     2761 gagaatctgg ggctttctta tccactggag ggggttaatc ctccgacgca ggaaaggatt
     2821 gataatgccc ggaagattct tggagctatc tgagtaaaaa tatatagctg tcatatcctc
     2881 tttttggggg tatggcagct ttgttttttg tttagaaaag gggacggacg cgaagcagga
     2941 gtatagtcgt tccaggtgcg gtttcggtat ctaagagatt ctaagccctc gaaaatccgc
     3001 taaaagcatg agacgaaaag tccggaatta ctcgctatcg ctcagacaag ggacttttcg
     3061 tctcataacg cagatttccg aggactaaga atctctaaga tgcctgcaaa tgcacctgga
     3121 atgactatac tcctgcttcg ctggttttgg ttaagatata atacaaggtg gagtgtgaat
     3181 tataagaagg actttgttta tataaaaatg aattgagatg tgaaatatat cccccgggag
     3241 ggcttgttgg ggggagggtg gatttgatat aattttggtt atatatgatt ataaattgcg
     3301 tgcatggagg ttggcataag gtgaaggatt tattatcgga gattgagagt tactggacta
     3361 cccgggctga ggggtattct gaagttaatc ataaggaatt gaatgggatg cagaaggggg
     3421 catggcttga ggtgttgaaa gggcagttcc cggagaaagc gaaggatgag attaagattt
     3481 tggatatcgg aacaggacct ggattttttc ctgttattct ggctgaggcg ggatataagg
     3541 tgactgctgt tgattatacg caggagatgt tggataccgc gaagaggaat gccggtaatc
     3601 tttgtgagcg gatttctttt tataagatgg atgctcagaa tctggaattt gaggatgatg
     3661 tgtttgatgt tgtgatcagc aggaatctga catggaatct gaaggatcct aagagagctt
     3721 atgaagagtg gtgtcgtgta ctgaaaccgg gcggaaagct tcttaatttc gatgcaaact
     3781 ggtacggtta tctttatgat gaggagaaac gcctgtctta tgaggaagac cgaaagagtg
     3841 tggagagtga gcatctggat gatcattacc tctgtacgga tattgacaga atggagaaga
     3901 ttgcactgca gatgcctctt tcttctatta acagaccgtc ctgggacagg aagtttctga
     3961 aggagaacgg gtttgagtct gtggcagtgg ataccgggat ctggcagcgg gtgtggagtc
     4021 aggaagagaa gctgaattat cattctacgc cgatgtttat gatcagcgct gtgaagaaag
     4081 agaaagatat ctggtccgag aatgacggaa cggatgattc tgatagcaga tatgacaggg
     4141 aaagagattt ggaggatgcc gcgttgtgta ctgctccggg tacgaaaaag agcggattcc
     4201 tgaaactggg cggaggtgag ttttcgcttc cttatacagt aatctgcggt tctcatcccg
     4261 gaaagacagt tctgatcacc gctgcggtcc acggaggaga atatgtggga atccaggctg
     4321 ctgttgagct tgcagataaa ctgaagcctg agaagatcca tggaagagtg atcctggtta
     4381 agactgtgtg caggaaggaa tttgaggaga ggagcggcag tatctgcccg gaggatgaga
     4441 agaatctgaa tcgtgtattt ccggggaatc cgcagggaac caggatggac cgtctggcgt
     4501 atgaggtggt tcagaagctt cacagtgcag cagattatta tattgatctt cacagcgggg
     4561 atgattatga gcagcttacg ccttatattt actatgccgg atgtgcagat gaagatgtgg
     4621 ttcagatgtc aaggaagatg gcagagcagg cagatgttcc ctatatggta aagtctaatg
     4681 tggcatccgg tggttcttac aattatgctg ccacatgtgg cattcccagt gttctgatcg
     4741 aacgtggcca gatgggaggc tggagtccgg aggaggttca ttctacaaga aaagatgtgc
     4801 gtaatattct gtgtgcgctg ggtgtttacg atggcatgag aagttacagt aattattatc
     4861 ccatggaaat tgaagatgtg cgttatcagt ctgccagtgt atccgggctg tggtatc
//
