LOCUS       c00402_gnlPro..         6615 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CLIHEBLN_402.
ACCESSION   c00402_gnlPro..
VERSION     c00402_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 18:38:36
            Original ID  :: gnl|Prokka|CLIHEBLN_402
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6615
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6615
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2687]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2687
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6615
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6615
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..446)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.6;
                     E-value: 1.1e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg402_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.8e-28, bitscore:
                     88.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTL"
     CDS             complement(447..2687)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg402_2"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.9e-283, bitscore:
                     927.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGIYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRS
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(2858..5161)
                     /locus_tag="ctg402_3"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKEVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERK
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             5318..6613
                     /locus_tag="ctg402_4"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLASDSSGITLAICKENRISCYTPSSDGFRDLPREGV
                     GPQTVRTLFAGTDGHFYLLLASGILKKLTPDFSDTTFNLLSEERQFHNKPILSAFYENN
                     HIFIVDTDNKLYRQHVDGKGKEFLFDLPEMTKQYGNIIKICTFQSNVYIVFRNGNILDL
                     SQPENTINMGIGIFCLMNDKRQEILWLGTDGQGIRMFYDKPDLFGSILLKDLPINIQNP
                     IRSLYTDDDQSLWLGTKGDGIVRIQAYDTYHNKKMIPQSAITHFTTADGLSSNRVYCFQ
                     KSEYHPCIWIGTEGPGLTYYSYKEKRIKTIPQR"
ORIGIN
        1 agaagcgtat tatcctgctt gacacccgcc agtaacggat gtaaatccgg gtttaaggtt
       61 ttgatgtcca ataacactaa gtccgcataa tccaagactt cccaagcttt tgaggtacat
      121 acgaaaccag atgtgtccaa agctgtgtga agaccttgct cttggcacaa acggaaaaac
      181 tctttcagaa actcaggttg caataaaggt tccccaccgg taacagtcac ccccccacgg
      241 gcgataaaac tcttataccg taagacttcc gtcaatagct cgccgggagt catctgatat
      301 ttcaccttcc ctttcgggtt ccaagtatcc gggttatggc aatacaggca acgcaacggg
      361 cagccctgca taaaaacgac aaagcgaatt ccgggaccat ctacggtccc gaaactctct
      421 aatgaatgta tcttaccttt tatcacttac agtgaatggt taatggttct tgaaataaca
      481 tccaactgtt gttcacgagt cagtttcacg aagtttaccg catatccgga tacacggatc
      541 gtaagttgcg gatatttctc gggatgctcc atagcgtcga tcaacaaatc cttatcaaat
      601 acgttaacat tcagatgctg acctccatcc ggagtaaaat agccatccaa taagctaacc
      661 agattattga tctgcacttc acgctccttt cccaacgtag ccggagaaac agcgaaagta
      721 taagagatgc cgtcatgcgc atgctggaat ggaagcttcg ctaccgaagc tagcgcagcg
      781 atagcacctt tcgtatcacg accgttcata gggttcgcac ccggagcgaa aggagttcca
      841 ccgatacggc catccggtgt cgtaccagtc ttcttaccat ataccacatt agaggtgatc
      901 gtaaggatgg actgcgtagg tttcgcatgg cgataggtct cgtgctgacg caaatattcc
      961 atgaacttct cggtgatctc tacagcgata ctatccgtac gatcatcgtt attaccgaaa
     1021 ggaacatatt cgccctcacg ttcgaaatca accgccaagc cacgctcgtc acggatcaca
     1081 cgtactttcg tatcacggat agcagccaac gaatcggcaa cgatagaaag accggcgata
     1141 cctgtcgcac ggatacgctc tacatcccca tcgtgaagcg acatctcgaa tgcctcatac
     1201 gcatatttat catgcatata atggattatc ttcaaggcgt tcacatacac tttcgccaac
     1261 cagcgcatca tttgctcata cttagccatc acctcatcgt attccagata ctcgctgcga
     1321 accggctcga acatcggggc tacctgtacg ccgctgcgct catcccgtcc tccgttgatc
     1381 gcatataata aacatttcgc caaattcgca cgggctccga agaactgcat ctgcttaccg
     1441 atcttcatag gagaaacgca acaagcgata ccataatcat ctccataatc cggacgcatc
     1501 aaatcgtcgt tctcatattg gatagccgac gtatcgatag agaccttggc acagaaacgt
     1561 ttccagttct cgggagcgtt cttaaaccac agaaccgtca aattgggttc tggtgccgga
     1621 cctagattat ataatgtatg gagataacga taggaagttc ttgttaccaa agaacgacca
     1681 tccactcctt ggccgcccaa ggactctgtt acccaaaccg gatcaccgga gaacaaatca
     1741 ttgtattccg gcgtacgtaa gaaacgaacg atacgcaact tgatgatcag ctggtctacc
     1801 aattcttgcg cctcctcttc ggtcaactta ccctcacgga tgtctttctc gatataaata
     1861 tccaaaaacg tagaagtacg tccgatagac atcgccgcgc catcttggtc ttttaccgcc
     1921 gccagataag ctaagtacac aaactgaacc gcctcacgag cgttttgtgc cggacgagtc
     1981 acatcaaagc catagctagc gcacattttc tcgaacgctt tcaaagcctt gatctgttcg
     2041 gaaatctcct cacggctctg gatgatctct tccgtgatct cttggatatc caaacgattc
     2101 aagaaacgct ttttctcctc gatcaagatt gccgtaccat ataaagcgac acgacgatag
     2161 tcaccaataa tacgtcctct accatacgca tcgggcagac cggtgataat agccgaacga
     2221 cgtgcggcaa tcatctcttc cgtatatgcg gagaaaacgc cctcgttatg agtcttacga
     2281 tacttcgtaa agatctcttt cgtcatcggg tccaaatgat aaccataagc ttccaagcta
     2341 ttctcgacca tacggatacc gcccttcggg aagataccac gtttcagcgg agcatcggtt
     2401 tgtaacccaa cgatcacctc attctcctta tcaatatagc ccgggccata tacatcgatc
     2461 ccttgaggta attttgtctc ggcgtcataa attccctttt ccctctcgac cttaaacatc
     2521 tcggtcagct tgttccagac cttacgagtc ttctcggaag aagcgactaa aaaggaatca
     2581 tccccttcat aaggcgtata gttactctgg ataaaatctc ttacattgat ctctcttttc
     2641 caggtctcac ccttaaagtg atcccagcac tcattctcaa atttcataaa caatcttatc
     2701 taataccaca tttttaccgc cgcaaaggta ggagaattat cgtcatattt ctgtcacata
     2761 tgctacacca gttctaaaat ccctataaat tgcgataaaa aagcttctca tccttcacta
     2821 ttttaaaagg acattaccaa taaatgggga gtcagaccta agggcgaaga ggcaaccatg
     2881 cccaaaaagt agatccgaca ccaagttttg aagtaaagcc cacttctccc cctgccgctt
     2941 ctacgatagc tctggagatc gtcaagccaa gtccggtacc ctgcgagaac tcattcaatt
     3001 tctcaaaacg ggtaaatact ttgtcatgca aatcatccgg tataccgatt cccgtatcct
     3061 caacatagat acgaagacct ttcctttcaa tgccatatcc cattttaata tatccggatt
     3121 tggtgcattt taccgcatta gtgaggaaat tcatccatac ttgcttaagc cggtttctat
     3181 ccagaagtac ccagcactcc gagttgggac catctaaacg taactcgaca ttcggatttg
     3241 tcatcttcgc ctgaatcgta acaaataact cattacaaac cttagccaga ttgaaattct
     3301 ccttcctacg ttcaattatg cctgactcta tcttggaaag atcaaggata tcgttgatca
     3361 atcgcaataa caactcatta ttggattgga tgatatttat atattctttt ttttcttccg
     3421 gatcatcaca agttaccatt aactctgaaa aaccaacaat agcgtttaac ggagtacgta
     3481 tctcgtgact catattagcg agaaacgcag acttgagacg atccgaaagt tccgctttct
     3541 cctttaattc cttcaattgc tgaatcatct tctcccactt cgtattattg atcgtaatac
     3601 cggtatagcg agtcacatgg ccttctttat ctctttcaac ctggattcca gtaaccaata
     3661 aggtctgcca ctcgtcatcc catttggttt tggaacgata tttaacgttg aaatctttat
     3721 ctatgccccg gaacatatgc tccatatatt tccgcacaaa aaccacatca tccggatgtg
     3781 tagcgttcaa ataatcctcg ggagtgattg tcttttccga ataaaaatca ttgataggat
     3841 cattaaaagc actgaatttc aggcttttta catcaaaatc ccaatacgac ataccaaccg
     3901 tcttgaaaga aagctctatc ttgtaatttc tttctttaag ttcccgattc aattgctgga
     3961 tcttaggaat gttttgcctg aatcccgtat agcgtattat atctccattt tcatcttgat
     4021 cgaaagggac acccataaaa tcacaatact gccaagtctc atcatacttg gtttgtatgc
     4081 gacaggtaaa attgatggtg acattctttc cagctatcag ggattgcatt gcgtcgtaaa
     4141 aaacagaccg gtcctcgggg tatataacat ccatatactc cgaaacggtc aataaccgat
     4201 ctgaaacgta atcgttgatc ggatcattat aagattcgaa tttctgggaa tttacatcaa
     4261 aatcccaatg aacaatatta ctgactttca tggccagatc acgttttgcg atcagatcct
     4321 gcgctttctt ggccatttgc aacctttcgg ttatatccat ttgtgtcccg atgatctgca
     4381 attttccacg atgttctgtc gataggcgca tccggctctc ataataacga tacatctctt
     4441 cttgctcgtt atagaaacgc aaagtcattt gtccttgttc tatctcattt cctatcaatt
     4501 gagagaagag ccggattaac ggagctcgat cttggggatg caaaatatcc agtaccgcct
     4561 ccattggcat attttttttg gctacggtta gtccatataa tggattaaaa acctttttat
     4621 tcacatcata tacccaagaa gccatattag ccgctttcat ggccatctcc agatttttct
     4681 tgctttcttc cgtacggaat tccacctctt tcacggaagt catatcattt gcgagtaata
     4741 tatgtccgat aagatgtttt tttctactcc atatcgggat aactttaacc ccatagatca
     4801 tcgtgtcttt attatgggaa gaataatatg catccttatt aacccgatca aaatcatact
     4861 caaactccaa ggaaatatct tcacccgatt gtatccgagc taaaagttct gaatcaacaa
     4921 atggactttt gtaaagattg actatattca ccaccgatgt ccggtcgtcc acaccataca
     4981 tcctcaacgc atggtcgttt atactacgca gcacaccatg tgcgtcataa atttcaatac
     5041 ccaccggcag acaagcatat atactctccg taaataaacg ctgttcttct attaatttag
     5101 aaatttcttt ctctattatc acatcgtcat tttcttgaga attctcttca cattgttcca
     5161 tctgtcactt actttatttc gtattatcat acgtcagaca ttctaattta tacgaaagcc
     5221 ctgctttttg caaaagtaaa caaaaaagtg aattcttttc tgttcgttca taaagatatt
     5281 atctttgcgc tgaaaacaaa cactaaatct taaatacatg agattcacaa ggcttattct
     5341 agcgttaacc agtttcctta tattgatcac gcaaacagac ctatttgcct caaccgtatt
     5401 gcataaaact tctatggttg atctctcaaa taacgctatt atttgcatgc atcaagatcc
     5461 ggatggctat ttgtggatcg ggacttacga cggacttaac ttatacaacg gaaaagatac
     5521 gtatgtatat cgtttcgaat taaataacaa aaactctctt tgtagtaata tcattcacaa
     5581 aatatccgac gcagaacccg gctttttatg gatatcaacc tctttaggta tcaataaatt
     5641 ctcactaaaa gaacggaaag tcaccgagtc tttccccgga tacatggaat gcgatctatt
     5701 agcgtccgat tcatccggca tcacgctcgc tatttgtaag gaaaacagga tctcttgcta
     5761 caccccatct agcgatgggt tccgggacct gccgagagaa ggagtaggcc ctcaaaccgt
     5821 acgtacctta ttcgccggga cggacggaca tttttattta ttattagcct cgggcatact
     5881 gaaaaagttg actccggatt tctccgatac tacttttaat ttgctatcgg aagaacgcca
     5941 attccataat aaaccgatct tatccgcatt ttacgagaat aatcatattt tcatagtaga
     6001 taccgacaat aaattatatc gacagcatgt agacggaaag gggaaagaat tcctgttcga
     6061 tctgcctgaa atgacaaaac aatatggtaa catcatcaag atatgcacct tccaatccaa
     6121 tgtgtatatc gttttccgta acggcaatat tctagaccta agccaaccgg aaaacacgat
     6181 aaatatgggc atcggtatct tttgcctaat gaacgataaa aggcaggaga ttctctggtt
     6241 gggaacagac gggcaaggta tacggatgtt ttatgacaaa cccgatcttt tcggctctat
     6301 cttactcaag gatttaccga ttaatataca gaaccccata cgttctttat atacagatga
     6361 cgatcaatcc ctttggctcg ggacaaaagg agacggtatt gtccggatac aagcgtatga
     6421 cacttaccat aacaaaaaga tgatcccaca aagcgctatc acgcatttta ccaccgccga
     6481 cggcctatcc agcaaccgtg tgtattgttt ccaaaaaagc gagtaccatc catgtatctg
     6541 gattggcacg gaaggcccgg gactgaccta ttattcgtat aaagagaaac gaataaaaac
     6601 gataccgcaa cgaga
//
