LOCUS       gnlProkkaDPJFMPLN_34   12100 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DPJFMPLN_34.
ACCESSION   gnlProkkaDPJFMPLN_34
VERSION     gnlProkkaDPJFMPLN_34
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 14:23:15
            Original ID  :: gnl|Prokka|DPJFMPLN_34
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12100
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..75
                     /locus_tag="ctg34_1"
                     /transl_table=11
                     /translation="MSMDMGEDRQRKMRKVSVLRGGKL"
     protocluster    1..12100
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3895:7100]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3896..7100
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..12100
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12100
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             62..892
                     /locus_tag="ctg34_2"
                     /transl_table=11
                     /translation="MGNFSLIIFKGWEPNKFCYSGIVLSVILCFFIFTQITELMKQSTA
                     TALTFWAFGNFLNRNWKKAIIIYFLALNIHFSCLYYFPLVFVFNLKNWIVPVIAVVSFI
                     FRQFNLMDSVVNILSNFPGIGDVMILGQIYSAAEIYRGEHETFFQSGAFMFTLIFYHFF
                     FLSFFSKIVVPKSNLVNVCLLMVVILNLSYTNNHNYTRLLTMMFPFYIMLFLELSAHQS
                     VFRKVLLYALVTITFIINFRFFFLRLFEASYTTSFVDNSILKLVLYPSFAYLFP"
     CDS             1086..1277
                     /locus_tag="ctg34_3"
                     /transl_table=11
                     /translation="MPSLVNRQVESIGEYLLLIVLYGGKLWFVYILFVMITVWTFLFIY
                     KGQGRMDNPWLICSRCVC"
     CDS             1403..1777
                     /locus_tag="ctg34_4"
                     /transl_table=11
                     /translation="MVFSLILFVPICLIFVVDIQPVILPVFKMLTKFIGCWFVWSLAFW
                     ILDVKPLSDALSYVGKNSLGYYWLNGFALVAARTAIFVLLNVESSVAIAVGVFATCVVI
                     ETIAVMIVMKIPYVGNLIGV"
     CDS             1809..2975
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 88.2;
                     E-value: 1.1e-26)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg34_5"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.3e-33,
                     bitscore: 107.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIIYIQDQEIDHYNGHFYHSKSEHFFSRFLAGLKENDTLTVYAG
                     IITRKSAHEVEKYKDVTNSRITYKQIPEFRNPKNLLVIKRMMQQILKDVDFCYLRSGIA
                     ASFASTICLKKHIPYLAVVNEDIFRNTVTHSRLLVRLSAYPLSYYTHRMIREANYACYV
                     TQEYLQSNYPCKGKSLGCSDIEYLDINEQSLVKRLDKIKKQEGTIVLGSVGSISTELKG
                     QDTVIKALAKLKAEGLTNYKFTMVGAGNPDRLRNLAIELGVENQIQFLGEFKHDDVLIW
                     FEDIDIYIHPSRSEGLPRTILEAMTKAAPCICSLVGGIPELIDQGSLFTYNGTEVDTLV
                     NIIKGFSKEKMEREARINFNRSKNYEPKLLEQRRNAFFIQAINEFRKN"
     CDS             3194..3871
                     /locus_tag="ctg34_6"
                     /transl_table=11
                     /translation="MFVEAYNYATHKGELGVFDVIDKTPQNFRIIIATPTHMSYPFVYK
                     YNGEYYMIPETGAAKEIVLYKATSFPEVWKRERVLLSGEVYRDTTVISNQDGTFTLLTY
                     RQVGTNSFTVKYYVEKFILDMNTMKIIRVDEYRDKKKINRPAGSLFSVDGEIYRVAQKC
                     SRAYGEAIFVYKTSKNFDFIKDKKVAELTGQSIVLSDGRKPILLHTYSQAGEIEVIDYR
                     CSL"
     CDS             3896..5206
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 190.9;
                     E-value: 4.6e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg34_7"
                     /sec_met_domain="CTP_transf_like (E-value: 5.9e-13,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.5e-114, bitscore:
                     371.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.8e-61, bitscore:
                     198.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTVYLGMIGDIMHPGYINIINKATEYGDVIIGLFTDKAIANHR
                     RLPYLTWEQRKNVVESIRNVSRVVPQDDWSYVSNLLKYKPDYIIHGDDWLVGPDKYIRD
                     EVFKVMEKLGGEVIEIPYTQNISASGIKQEIDALGVTPQMRLSSLRRLIAAKPIVRILE
                     SHNGLTGLIAEHTKVEVNGQEREFDGMWASSLTDSTSKGKPDIEAVDLTTRLHDLNDTL
                     EVTTKPVIFDGDTGGKIEHFGFTVRTLERLGISCVIIEDKVGLKQNSLFGTDAVQTQDT
                     IEGFQAKIRAGKEAQITRDFMIVSRCESLIAGKTVDDALERCHAYVEAGTDGIMIHSKE
                     KSGEDIKEFCLRFREKNAHTPIIVVPTTYNQFTEEELAEWGINVVIYANHMLRSAYPAM
                     VKCAETILYTSRSLEASEQYCMPIKQILNLIPGTKKK"
     CDS             5241..6368
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 74.7;
                     E-value: 1.1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg34_8"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.1e-20, bitscore:
                     63.8, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.2e-23, bitscore:
                     74.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRPEFFVNILKEQGIDFYAGVPDSLLKNICAYITDHLPAEQNII
                     AANEGGAMGIAVGYHLATGKVPVVYMQNSGEGNIINPFASLTDKDVYNIPVLLVIGWRG
                     RPGVHDEPQHVKQGKVTIGLLNTMGINYAILPKDEEGAAKQIKIAVDFMKATNECYALV
                     IEKDTFDTYKLQSVEVNELAMSREETIQKVASNIEDNACIVSTTGMISRELFEARTAWS
                     QGHERDFLTVGAMGHASQIALGIALQKPERRVYCFDGDGASIMHMGNMAITASMKCKNY
                     VHIVFNNGAHDSVGGQPTVGLKIDFCAIAQAVGYKAAYSVETMEALEAKLAEFKNAEGP
                     VFLQICVKKGNRKDLGRPTTTPIQNKEAFMEFLNK"
     CDS             6372..7100
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg34_9"
                     /sec_met_domain="Metallophos_2 (E-value: 1.5e-27, bitscore:
                     88.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVILFADIHANLSAFEAVLADIEKHYQPDAMISLGDFIDYGMRS
                     NEIIERIQTVNIPLLANLKGNHEKALIDGELSCFSSERARVMSKYTRNHLTQTSIDYIS
                     NVMQEPMQELEIGGKKVLLLHGTIDDPYWGKLTPAKVEDEKYQDFDYVLSGHIHQPFKI
                     DVFFKVDNPIMRNQKRTTFINPGSVGQPRNQNPYAQYVYINFKTGETHFNAVPYDIEAE
                     QSLFPKEIDTFYSERLKFGI"
     CDS             7101..8096
                     /NRPS_PKS="Domain: NAD_binding_4 (83-198). E-value:
                     6.4e-10. Score: 30.4. Matches aSDomain:
                     nrpspksdomains_ctg34_10_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     143.1; E-value: 2.2e-43)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg34_10"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.2e-09,
                     bitscore: 27.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.1e-08, bitscore:
                     25.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLYVISGVTGMTGSELSRQFVSQGHTVIGFDNFFASSIDTVKD
                     IIDNPSFIFYNYDINKVAQMDEVCDKAKELKIECDGRLVFINCAAVVHTKHFYEVESTF
                     QTNVLAMKDFLERAISMRADTYINCSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRT
                     SYACGKLMTEFFMKDAVGKGRIKGCSIRFANVYSRHERFAEHIIPHIIDNLLESHTVTL
                     LENSKVNKRTFLHNIDSCRAVMELIASEDALDGTVYNVATEEEIAIIDLVKTIAIKMGI
                     NDVDIRFEGYRKSDPERRLLNTDKIRTRTSWKPIITLDEGLTMCVNSIKK"
     aSDomain        7350..7694
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg34_10_NAD_binding_4.1"
                     /evalue="6.40E-10"
                     /label="ctg34_10_NAD_binding_4.1"
                     /locus_tag="ctg34_10"
                     /protein_end="198"
                     /protein_start="83"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VFINCAAVVHTKHFYEVESTFQTNVLAMKDFLERAISMRADTYIN
                     CSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRTSYACGKLMTEFFMKDAVGKGRIKG
                     CSIRFANVYSR"
     CDS             8093..8833
                     /locus_tag="ctg34_11"
                     /transl_table=11
                     /translation="MKYLIITVAGTATRFNRDTKEETLKCLYYTDEPQYALLAQLLKNC
                     GEYDKYILVGGYLYEKLGRFVKNELSGYGKKIELVYNEHFKDYGSGYSLYKGIEAIKEA
                     GDVTFVEGDLFFMATDFRQVYNSPKSVITINREPIYSNKAVALYINVDGKPRYLYDTNH
                     QTLTVSEPFKAIFNSGQMWKFSSSERLLKTVDSLTKEQLQGTNLEIIQAYFSDMNSEEY
                     DVVTFTDWFNCNTVADYDIVRKLM"
     CDS             8836..9321
                     /locus_tag="ctg34_12"
                     /transl_table=11
                     /translation="MNMAVLERDYRIDGIKWLLIVLVTFGHVIEPALSNPIANKLYSII
                     YIFHMPLFVFISGYYANVKDKEKLISKGIMLLETFLVVMIPQCFYYGSIIPLLNPENSG
                     WYLISLITWYIIVFLLITNKLVGKIVVRYDGDEGGEVVDIQLLVGRICIHASVETIW"
     CDS             9296..9856
                     /locus_tag="ctg34_13"
                     /transl_table=11
                     /translation="MLPLKQFGNLLSFQRSCMFLPFFMMGFLRRVKGLPIIPDVSKCIK
                     LVLAIASLVCFAFCLIYSGRILHVLEFNNANVWHMSNLYNQNIGMLLVLKVLVYIGSII
                     TTFAVLMFVNLPKSICAYGSKTLAYYVIQGMFAHILSTYLQVNFYQALFLCVCILVLTT
                     VLLRFVDSKFITNPFSTLLKWKY"
     CDS             9844..11031
                     /locus_tag="ctg34_14"
                     /transl_table=11
                     /translation="MEILNRKLSLLKGKVENSQPITIMIIGLGSVGCYLLDYLVSLGDS
                     QLRLVVVGRNAEKMQMDINIIRTASTIRHQCRSKIKVVDNCDLNDVNSIAAVLEAEKPD
                     FIVNSSRVYSGLKYGSISWSNLRAYGIWTPLSIRYAKNIMEAYDKANCEAISINTSYSD
                     AVIPWLKSAGKAYFDFGSGNLNHLVPRIKFYIAEKYGIKNFNDIDVTIAVSHFHDVVIS
                     KEGHAEGQDILLDIKFQGKDMDFNKEELLKSCSIAMPVDQKRNMMNASSNFDIIFSVLT
                     ALREEKQVKIHTPGVNGEIGGYPIIIDGVTATAKFDESVWTIDQMRKANRESIYCDGVE
                     NITDATLVYTDELVAKVKKSFNVDLPKSVKFVDIENVADLIINQIIKPQVFVFVQ"
     CDS             11179..11328
                     /locus_tag="ctg34_15"
                     /transl_table=11
                     /translation="MYSSEDLASFYFQYQTKALPHVKFLQSFCMKHKVPIIFFISGIRI
                     RVIK"
     CDS             complement(11438..11761)
                     /locus_tag="ctg34_16"
                     /transl_table=11
                     /translation="MSCVHIVSIDAFKIECSFHNYVRHPNSSPQGYIEILKAVDVNRIW
                     NEWIFLGILKSPIYDIVLETFILQHGLINHSRTIGIMTSTITYKKHEFTVWLQEQLVQI
                     YQT"
ORIGIN
        1 cagtcaatgg atatggggga agacagacaa aggaaaatga gaaaagttag tgttttaagg
       61 ggtgggaaac tttagtctta taatatttaa aggatgggaa cccaataaat tctgctattc
      121 aggtatagtg ctgtctgtaa tactgtgctt ctttatcttt actcagataa ctgaattaat
      181 gaagcaatct actgctacag cactcacatt ttgggcattc ggtaatttct taaatagaaa
      241 ctggaaaaaa gcaataatca tttatttttt agcattaaat atacattttt catgtctgta
      301 ttactttcca cttgtttttg tttttaattt gaaaaactgg atagtgcctg tgatagctgt
      361 ggttagtttt atatttagac aattcaatct tatggattct gtcgttaata ttttaagtaa
      421 tttccctggt attggtgatg ttatgatatt aggacagata tattctgctg cagagatata
      481 cagaggagaa cacgaaactt tttttcaatc gggagcattt atgtttactt tgatttttta
      541 tcatttcttt tttttatcat ttttttctaa gatagttgtt cccaaaagta atttggtaaa
      601 tgtatgtttg ctaatggtgg ttatccttaa tttaagctat actaataatc ataactacac
      661 gcgtcttctt acaatgatgt ttccatttta cataatgcta tttttagagc tatctgccca
      721 tcaaagtgta tttaggaaag tgcttcttta tgcactggta actattacat ttattattaa
      781 ctttcgtttt ttctttttga gattatttga agcgtcatac acaacatcat ttgtagataa
      841 ttcaattctt aaattagttt tatatccgtc ttttgcatat ttattcccat aaaaagattt
      901 tgaatccaga ataggtatta gaaatcttta atggctgcag ggattatgtt gatatgattc
      961 ctggcaagat cactttggct actaaccaat gtaaattcaa gactaattaa ttaataaaac
     1021 tcggcttagg gcacagtaat gtgccctttc aaagggggat gccctaaatt gagtgtttac
     1081 tggcaatgcc atcgctggtg aatcgtcaag tggaatctat tggcgaatac cttttgttga
     1141 tagtgttata cggaggtaaa ctttggtttg tctatattct gtttgttatg ataacggtat
     1201 ggacgttcct tttcatatat aaggggcagg ggaggatgga taatccttgg cttatttgca
     1261 gtagatgtgt ttgctaattc tcacattcac gacaatatcc ttttagtgct ttatttggcg
     1321 cattattcta tgttctttct tataggccat ttcttgcgat aagttgaatg gtttcgttct
     1381 ttcattcaga gcaaaaaaaa ttatggtgtt cagtttgata ctttttgttc caatatgctt
     1441 aatatttgtt gttgatattc agcctgttat tttacctgtt tttaagatgt tgacaaagtt
     1501 catcggctgt tggttcgtat ggtctttagc attttggata ctagatgtca agccattaag
     1561 cgatgctctt tcgtatgtag gcaagaattc attgggctac tattggttaa acggctttgc
     1621 ccttgttgca gcacgaactg ccatattcgt gttgttgaac gttgaatctt cagtggcaat
     1681 tgccgtaggc gtatttgcta catgtgtagt aatagaaaca atagccgtaa tgattgtaat
     1741 gaagattcca tacgtaggta atttgatagg tgtgtaaggc ataagaatca gcaaaaaatc
     1801 ccattataat gaatataata tatattcaag atcaggaaat tgaccattac aatggtcatt
     1861 tttatcactc taaatcagaa cactttttct ctagatttct tgcgggattg aaagagaatg
     1921 atactttgac tgtttatgca ggcattatca ctcgaaagag tgctcatgag gtggaaaaat
     1981 ataaggatgt aacaaattct cgtatcactt acaagcagat accggagttt cgcaacccca
     2041 aaaaccttct tgttatcaaa cgcatgatgc agcagattct aaaagatgtg gatttttgct
     2101 atttgcgtag tggtattgca gcttcgtttg ccagtaccat ttgccttaag aaacacatcc
     2161 cttatttagc tgtggtgaat gaagatatct ttcgcaatac tgttactcat tcacgtctgt
     2221 tggtacgttt gtcggcctat ccactctcgt attacactca tcgcatgatt cgagaagcca
     2281 attatgcgtg ctatgtgacg caggaatatc tgcaaagcaa ctatccttgc aagggcaaaa
     2341 gccttggctg ttcagacatt gaataccttg acattaatga gcaatcgttg gtgaagcggt
     2401 tggataaaat caagaagcaa gaaggaacta tcgtcctggg ttctgtaggg tcaataagta
     2461 cggaattgaa aggacaagat acagttatca aggcattggc gaaattaaaa gctgaagggt
     2521 tgaccaacta taagtttact atggtaggag caggtaatcc cgatcgcctg cgcaaccttg
     2581 ctattgaatt aggagttgag aatcagattc agtttctcgg agagttcaaa cacgatgatg
     2641 ttctaatatg gttcgaggat attgacatct atattcatcc aagtcgttca gaaggtcttc
     2701 caagaaccat ccttgaggct atgactaagg cggctccgtg catttgctcg ttagtaggag
     2761 gtattcctga actcatagat caaggttcgc tttttacata caatggtaca gaggtcgata
     2821 ctttagtgaa tatcattaaa ggcttcagca aagagaagat ggaacgagaa gcaagaatca
     2881 actttaatcg ttcgaagaat tatgaaccaa agttgcttga gcagaggcgt aatgccttct
     2941 ttatacaggc tatcaatgaa tttcgcaaaa attaaatgat aatagatact ttatgaattt
     3001 atattactcc cgaatttgtc gcaaacttgg acttgctaaa acgacagtat ggggaattgc
     3061 atatcgaaag gcaactaatt ttgatggtat tctgaccaat aaaagaaaag aagaaccttt
     3121 tgaaatcctt ccaaatacag atgagttttg gtttgcggat cctctgttat ttgaggacaa
     3181 tggtaaaatt tggttgtttg ttgaagccta taactatgct actcataaag gtgaattagg
     3241 ggtattcgat gtgatagaca aaacgccaca gaatttccga atcataatag ctactcctac
     3301 tcacatgagc tatccatttg tctataagta taatggcgag tactacatga tacctgaaac
     3361 aggagcagcc aaagagattg tactctacaa ggctacttct ttcccggaag tttggaagcg
     3421 agagagggtc ttgctttcag gagaagtgta tcgtgacaca actgttattt ctaatcaaga
     3481 tggtacattc actcttttga cttatcgtca ggttggaaca aatagcttta cagttaagta
     3541 ttatgttgag aagtttattc ttgacatgaa tacgatgaag ataattaggg ttgacgagta
     3601 tcgcgacaaa aagaagatta atcgtcccgc aggttcgtta ttctctgtag atggagaaat
     3661 ttatcgagtg gcacaaaaat gcagtcgtgc atatggagag gcgatctttg tgtataagac
     3721 aagtaagaat tttgatttta ttaaagataa aaaggtagca gaactcacag gtcagagtat
     3781 agtcctgagt gatggtagaa agccgatttt gttgcacact tattctcaag caggagaaat
     3841 tgaggtgatt gactatagat gctctttata ataatttgaa aaatattaaa taataatggc
     3901 aaagacagtt tatcttggta tgataggcga tatcatgcat ccgggttaca ttaacatcat
     3961 taacaaagct actgagtatg gcgatgtgat aatcggtttg ttcactgata aggcgattgc
     4021 taaccaccgt agacttcctt atctcacttg ggagcaaaga aagaacgttg ttgagagcat
     4081 caggaacgta tcaagggtgg tccctcagga tgattggagc tatgtctcta atctccttaa
     4141 gtacaagcct gattacatca ttcatggtga cgattggctg gttggccctg acaagtatat
     4201 ccgtgatgag gtattcaaag taatggaaaa gttgggtggt gaggttatcg agattcctta
     4261 cacccaaaac atctctgctt ctggtatcaa acaagagatt gatgctcttg gtgtaactcc
     4321 tcagatgcgt ctttcttctc tccgtcgcct tattgcagct aaaccgattg tacgtattct
     4381 tgaaagtcac aatggtctta ctggcctcat agcagaacat actaaggtag aggtgaatgg
     4441 tcaggagcgt gaattcgatg gcatgtgggc ttcctccctt accgactcta cctctaaggg
     4501 taagcctgat attgaggctg tcgatttgac tacccgtctc catgacctca atgataccct
     4561 tgaagtaacc acaaaacctg ttatcttcga tggcgatact ggtggcaaga ttgaacactt
     4621 cggttttacc gttcgcaccc ttgagcgctt gggaatctct tgcgttataa ttgaagataa
     4681 ggtaggcttg aaacagaatt cactttttgg tactgatgct gtacagactc aggatacaat
     4741 cgaaggtttt caggctaaaa tccgtgcggg taaggaggcg cagattactc gtgacttcat
     4801 gattgtcagc cgttgtgagt ctctcattgc tggtaagact gtagatgatg ctttggagcg
     4861 ttgtcatgct tacgtagagg caggtactga cggtataatg atccactcca aagagaagtc
     4921 tggagaggac atcaaggagt tttgcttgcg ctttcgcgag aagaatgctc atactcctat
     4981 catcgttgtg cctactactt ataaccagtt cacagaagag gaacttgctg aatggggtat
     5041 taatgtagtg atttatgcta atcacatgct tcgttctgct tatcctgcaa tggttaaatg
     5101 tgcagaaacc atcctctata ctagccgttc gcttgaagca tctgagcagt actgtatgcc
     5161 tatcaaacag attcttaatc ttattccagg cacaaagaag aagtaaacat taatttctta
     5221 ccccttaaac tattttacaa atggtaagac ccgaattttt tgtaaatata ctcaaggaac
     5281 aaggcataga cttttatgct ggtgttcctg attcgttgct caagaacatt tgtgcatata
     5341 ttaccgacca ccttcctgct gagcagaata ttattgctgc taacgaagga ggggccatgg
     5401 gtattgctgt aggttatcat ttggcaacag gtaaggtgcc tgtagtttat atgcagaact
     5461 ctggtgaagg caatatcatt aatccgtttg cctcccttac cgacaaggac gtttataaca
     5521 ttcctgtgct cctcgttatc ggttggagag ggcgcccagg agttcacgat gaacctcagc
     5581 atgtgaagca aggtaaggtg acaatcggtt tgcttaatac catggggatt aactatgcta
     5641 ttctgccaaa ggatgaagag ggagctgcaa agcagataaa aatagctgtc gacttcatga
     5701 aggcaaccaa cgagtgctat gcccttgtga tagagaagga tactttcgat acctataagc
     5761 ttcagagcgt tgaggtaaac gaactggcaa tgtctcgcga agagacgatt cagaaggtgg
     5821 cttcaaacat tgaagataat gcttgtatcg tatcaactac tggtatgatt tctcgcgagc
     5881 tctttgaggc acgtactgct tggagccaag gtcatgaacg tgatttcctt actgtagggg
     5941 cgatgggcca tgcttcccag atagctctcg gcatagctct ccaaaagcct gagcgtcgag
     6001 tatattgttt tgatggtgat ggagccagca tcatgcacat gggtaatatg gctattacag
     6061 ccagcatgaa atgcaagaat tatgtgcaca ttgtgttcaa caatggcgca cacgattctg
     6121 ttggtggtca gcctactgtt ggtctcaaga ttgatttttg tgcaattgct caagctgtag
     6181 gttacaaggc tgcttatagt gtagaaacta tggaggcact tgaagcaaaa cttgcagaat
     6241 tcaagaacgc agaaggtcct gttttccttc agatttgcgt aaaaaaggga aaccgcaagg
     6301 accttggtcg tccaactaca actcctattc agaacaaaga ggcttttatg gaatttctta
     6361 acaagtaaag tatgaaagta attctgtttg ctgatataca cgccaatctt tctgcttttg
     6421 aggcggttct agctgatatt gagaaacact atcagccaga tgccatgatt agtctaggtg
     6481 actttatcga ctatggtatg cgttcaaatg aaattattga acgtatacaa actgttaata
     6541 ttccattact tgccaatttg aagggcaatc acgagaaggc tttgattgat ggggaactct
     6601 catgtttctc ttcagaaaga gctcgtgtga tgtccaaata cacaagaaac catctcactc
     6661 agacatctat tgattatata agcaatgtga tgcaagagcc aatgcaagag ttggaaattg
     6721 gtggtaagaa agtacttctt ttacatggaa ctattgatga tccctattgg ggaaaactga
     6781 ctccagcaaa agtggaagat gaaaagtatc aggattttga ctacgtattg agcggtcaca
     6841 ttcatcaacc ttttaagata gatgtattct tcaaagttga caatccaatc atgcgcaatc
     6901 agaaacgtac gacttttatc aacccaggtt cagttggaca acctcgcaat cagaatccat
     6961 atgcacaata tgtgtatatc aattttaaaa cgggggaaac ccatttcaat gcagttcctt
     7021 acgatattga agctgaacaa tctcttttcc ccaaagagat agatactttt tacagcgaac
     7081 gattaaaatt tggaatataa atgaagaact tatatgtaat aagtggtgtt actggcatga
     7141 cgggtagtga actgtctcgt cagtttgttt ctcagggtca tacggtaatc ggtttcgaca
     7201 acttctttgc aagttctatt gataccgtta aagacattat tgacaatccc tcgttcatct
     7261 tctataatta cgacattaac aaagtcgctc agatggatga agtatgcgat aaggcgaaag
     7321 aactcaaaat tgagtgcgat ggccgtctgg tgtttatcaa ttgtgctgct gttgtccata
     7381 caaagcattt ctatgaggta gagagtactt ttcagactaa cgtccttgct atgaaggatt
     7441 tcttggagcg tgccatttct atgagggctg atacttacat taattgttca acttcggagg
     7501 tttactcaat gcagtcttgg aatgagaatg gcggtgtgaa agaatctgat tatttggcaa
     7561 tgtcaacagc ggaacatagc caacgtacaa gttatgcttg cggcaagttg atgactgaat
     7621 tctttatgaa agatgctgtt ggcaagggac gaattaaagg ttgttctatt cgctttgcta
     7681 atgtatatag caggcacgag cgttttgcag agcacattat ccctcatatc attgataatc
     7741 tgcttgaaag ccataccgta acccttcttg aaaactcgaa ggtgaacaaa cgtaccttcc
     7801 ttcataatat tgatagctgt cgtgccgtta tggagctcat cgcttctgag gatgctcttg
     7861 atggtactgt ttataatgtg gctactgaag aggaaattgc cattattgat ttagtcaaga
     7921 cgattgcaat aaaaatgggc atcaacgatg tggatatccg tttcgaaggt taccgaaagt
     7981 ctgatcctga gcgtcgattg ctcaatacgg acaagattcg tacccgtact agctggaagc
     8041 ctatcatcac tctcgatgaa ggcctcacga tgtgtgtaaa tagtatcaag aaatgaaata
     8101 tttgattata actgttgctg gaacggctac gcgttttaat cgcgacacta aggaagaaac
     8161 cctcaaatgc ttgtattata ccgatgaacc tcaatacgct cttcttgctc aattgctgaa
     8221 gaattgtggt gagtacgata aatatatcct tgtggggggc tacctttatg agaagttggg
     8281 aagatttgta aagaacgaac tctctggtta tggcaaaaaa atagaacttg tttacaacga
     8341 gcatttcaaa gactatggct ccggctacag tttatacaaa ggtatagagg caataaagga
     8401 ggcaggtgat gtgactttcg tagaaggcga cttgttcttt atggctacag atttcaggca
     8461 agtgtataat tcgccaaaga gtgtgattac cattaaccga gaaccaatct actcgaataa
     8521 ggcagttgcg ttatacatta acgttgatgg caaaccaagg tatttatatg atacgaatca
     8581 tcagacatta actgtatcag agccattcaa ggcaatcttc aacagtggtc agatgtggaa
     8641 attttcttca tctgagaggc ttttgaagac tgttgattcg cttacaaaag aacaacttca
     8701 aggtactaat cttgaaataa ttcaggctta ctttagtgat atgaatagtg aagagtatga
     8761 tgtagtgact ttcactgact ggttcaactg caatactgtt gcagattatg atattgtaag
     8821 aaaattgatg taaaaatgaa tatggctgta ttagaaagag attacagaat agatggaata
     8881 aagtggttgc tcattgtatt ggttacattc ggtcatgtaa tagaacctgc tttaagcaat
     8941 cctattgcca ataagctcta ttctataatc tacatttttc atatgccatt attcgtcttt
     9001 atcagtggtt attatgcaaa tgttaaggat aaagaaaaac tgattagtaa aggtattatg
     9061 cttttggaaa catttttggt tgtgatgatt cctcaatgtt tttattatgg aagtatcatc
     9121 cctttactaa atccggagaa ctcaggctgg tatttaatca gcctaataac ttggtatatt
     9181 atagtttttt tgttgattac caataagcta gttggcaaaa ttgttgtacg atatgatgga
     9241 gatgaggggg gggaagttgt tgatattcag ttgcttgttg gccgtatttg catacatgct
     9301 tccgttgaaa caatttggta acctgttatc gttccaacgg tcgtgtatgt ttctaccatt
     9361 ctttatgatg ggtttcttga gaagagtaaa aggcctgcct attattcctg atgtttcaaa
     9421 atgcataaag cttgttttgg ctatagcctc tttagtatgt tttgcttttt gtctcatcta
     9481 tagtggtcgt atactacatg tattagagtt taataatgcc aatgtgtggc acatgtcaaa
     9541 tttgtacaat caaaatatag gaatgcttct tgttttgaaa gtattggttt atattggatc
     9601 aattattact acattcgctg ttttaatgtt tgtgaacctc cccaagtcga tatgtgctta
     9661 tggatcaaag actttagctt attatgtaat acaaggcatg tttgcacaca tattatcaac
     9721 ctatttgcaa gttaattttt accaggcctt gtttctctgt gtttgcatat tagtccttac
     9781 aacagttcta ttacgatttg ttgattctaa atttattaca aacccgtttt ctactttatt
     9841 aaaatggaaa tactgaacag aaaactttcc cttctcaaag ggaaggttga aaatagtcag
     9901 ccaataacta ttatgattat tggtttgggt agcgttggat gctacttgtt ggattatctt
     9961 gtatcactag gtgattcaca acttcgtctt gttgtggtag gacgtaatgc cgaaaagatg
    10021 cagatggata taaatattat tcgtactgct tcaaccattc gtcaccagtg ccgtagcaag
    10081 attaaggtgg ttgataactg cgaccttaac gatgtgaaca gcattgcagc tgtacttgaa
    10141 gcagaaaagc ctgacttcat agtaaatagt agtcgagttt actctggttt gaagtatggt
    10201 agcatctcat ggagtaatct tcgtgcatac ggcatttgga ctccgctttc tattcgatat
    10261 gcaaagaata taatggaggc atacgataag gccaactgtg aggcaatctc aatcaatact
    10321 tcatattctg atgctgttat tccttggcta aagtctgcgg gtaaggctta ttttgacttt
    10381 ggtagtggta atttgaatca tcttgtacct cgtataaaat tctatattgc agagaaatat
    10441 ggtataaaga attttaatga cattgatgta actattgcgg tatcacattt tcatgatgtt
    10501 gtcatttcta aggaaggtca tgcagaaggt caggacatct tgcttgatat aaagtttcaa
    10561 ggcaaggata tggattttaa taaggaagaa cttcttaaat cctgttctat agcaatgcct
    10621 gtagatcaga agcgcaatat gatgaatgct tccagtaatt ttgatatcat attctctgtt
    10681 cttactgctc ttcgagagga aaaacaagtc aaaatacata ctcctggggt aaatggtgaa
    10741 attggtggtt atcctataat tattgatgga gtaactgcta ctgccaagtt tgatgagtct
    10801 gtatggacta tagaccagat gcgtaaagct aaccgtgagt caatttattg tgatggagta
    10861 gagaatataa ctgacgctac tctcgtctat actgatgaac ttgtagctaa ggtaaagaag
    10921 tcgtttaatg ttgatttgcc taagtcggta aagtttgtgg atattgaaaa tgtagcagat
    10981 ttaattatca atcagataat taaacctcaa gtgtttgttt ttgtgcaata atttgtaaac
    11041 taatggcaga taatagattg ttgttaattt tctgtataat ctttaactta tagttttatg
    11101 atatacacag ctatgtaagc actttaaaaa gttcaaaatg aaatgttagc cttcgttgta
    11161 aattaaaatt agcaagatat gtatagcagc gaagatttag caagttttta ttttcagtac
    11221 cagaccaagg ctttacctca tgtcaagttc ctccagtcct tttgtatgaa gcataaagtt
    11281 cctataatat ttttcataag tggtataagg atacgcgtaa taaaataatt acagttctat
    11341 atctccgatt gttcaaatga aaaatgtaat aaatatggat atcacaacaa agataaaaag
    11401 aagtaaatta ctttattacg tctactatta cgtaatgtca ggtttggtaa atttgtacaa
    11461 gttgttcttg aagccagacg gtaaactcat gctttttgta tgttatggtg gacgtcatta
    11521 ttccgatagt acgcgagtga tttatgaggc catgttgaag gatgaacgtt tcaaggacta
    11581 tatcatatat tgggctttta agaatcccga gaaatatcca ttcattccaa atacggttaa
    11641 cgtcaacagc cttaagtatt tcaatatagc cttgagggct agagtttggg tgacgaacgt
    11701 aattgtggaa cgagcactca attttaaagg catcaatact tactatatgc acacaactca
    11761 tggagtatta tgtaagttgg atggtaaaga tgctattgat tctgctaatt tcaggacttt
    11821 atcaacattc aagtgggatt gctgttttgc atcttctgat attgagaagt atgtgtatgc
    11881 tggcatgttc ggaataccaa taggcaagat gcacgtaatc ggtatgccta agaatgatat
    11941 tttggtgcat tatacggaag actacagaag tgaattgcgc aagagaattg gaattccaga
    12001 aggaaagaag gctattttgt atgcaccaac atttagagaa gaggctaact ttaaagaggt
    12061 atttgatgtg gatgtaaacc tttggaaaca gaatctgggc
//
