LOCUS       c00227_gnlPro..         7210 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JELABEBK_227.
ACCESSION   c00227_gnlPro..
VERSION     c00227_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 14:38:00
            Original ID  :: gnl|Prokka|JELABEBK_227
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7210
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7210
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3925:6898]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3926..6898
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7210
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7210
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(148..2265)
                     /locus_tag="ctg227_1"
                     /transl_table=11
                     /translation="MEHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPIFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSSEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(2353..2583)
                     /locus_tag="ctg227_2"
                     /transl_table=11
                     /translation="MYYPDQPFHICTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             2777..3280
                     /locus_tag="ctg227_3"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             3301..3468
                     /locus_tag="ctg227_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             3455..3898
                     /locus_tag="ctg227_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVNELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3926..4654)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_6"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4670..6898)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_7"
                     /sec_met_domain="Gly_radical (E-value: 3e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ggtgagggta ggtgagggta aatttctaac attcgtattt tttttttaga agaaaataga
       61 agaatagaaa taagaaatat aaaaatgaaa agttgcaaaa gtaccctcac caaccctcac
      121 ctttttgcgt gcgaaggaga tgaaatctta ttcatatagc ctgtttgatt tcacctgttc
      181 cgtatcaata atgaatacag tgtatcttct cccgtttttt cccttatgaa attcgaagcc
      241 gtgtttggac atcacttttc ctagttgggc tttggttcca tgagaatatt gataccccgt
      301 ccgttcgcga atcaattccg caatttcagt gaccgtcaga taatgcacct tttcgaaacg
      361 ttccggtttg cggatatgtg tcaacaccag ttcttcttcc gatgagtgga aaatgaaatc
      421 ctcgttgttt tcttcaatcc gtgagttttc cgactgggtg aaccaatact gataccccgg
      481 cttgttgagc agatacttga tttgtgcata caactgcgcg tagtttattt tgataaactc
      541 catcgagttg acatgaaagc agagaaaacg gcggttacct gtggtgtcgt gaagcacttc
      601 ctgataattg catgtgccgg caaaagaagc cgtgtgaggg aagttctgtg aacgccgcgc
      661 gtatggcagg cggatgctga ttactttgcg ggtaaccaaa tccttgaaga tattcagttc
      721 ccgtccgttc atcccttcaa actcatccag attgataagc attgcctgcg ctattttggc
      781 cagatcatcc ttgttgttcg ggttaataag accggtagaa agataggcgc gcaattcggg
      841 cggcagcagg ttgttgataa acgtagtctt tccgatgttc tgtatttcgc tgcaaagcaa
      901 caaaacagtg tggttcacca catcgtcttg cgtggcggca gcgcacattc ccaccagata
      961 acgttccaga cattctaccc aaaatttctg gtgactagta tgcacgctgt tggcaaggat
     1021 accgatataa tccgttccgt cccattcagg cagcgagtct aaatactcgc gaataggatg
     1081 gtaggtgctg ctgaagtcgg agtagatcag attctgaatc gtcttgacgg tgcacgaata
     1141 gcccagttcg ttcatctcca tccaaatgct gttttccatc atttcgtcga gaatatggaa
     1201 aggttccggt tgctccgtgt ccgttttcct tctgcgatat tccatgatat gaagcacttc
     1261 gttgtagcgt gtatcgtaat tttctttgat atgttgctca atctgtaata ttcgcttctg
     1321 cgtactattc agcttgcggg tgtcgaattc ctcattgtgt ccgtaagcac tcccgatcag
     1381 tgcgtccatt tcatccgcgg gaagatcggt gaactggctc ttgataaaag ctgccgcctc
     1441 ttcctggggg acgccgtagc gattgtacat acaagccagg caatgcaggt agttgttccg
     1501 gttgccggtg acgtactttt cggatttatt gtgataatag ttcagagtca ccattaagga
     1561 agcgtgcgag gaatggtagt tgagtgccac ttgttcggtg aggggattgt tgtctggtgc
     1621 tgtcgtcttt ttctttttgc cggatgccct tttcttcttt tgagacttga agaatatcgg
     1681 tggctgttcc acgatgacag ctgtggcatc gggattgaaa taaatatccg gatcgtaaga
     1741 gaacaggcaa gtacggctga catcttgtcc ggaagtgtcg acctcaactc cgcagagctg
     1801 ggcataaaag gaagcgattt tgtgataagc ggcatgatgg aaatcttcaa tttcctgaat
     1861 cgtttcgggc aatgtcccgt ccgtccggca tacacgtacc acgattttca gtcccattcc
     1921 tttgggactg atgaaagcga tgcgggtgtg ggcacacttt tttgccagtg cgaggatgcg
     1981 tgccaatgct tcctgtgtct gatggtcgat gtctaccacg atgtatccca gatacttgac
     2041 gagagttgcg tttgaacgtc ctccccggta gttggctgaa aaggtgattt ccggtagttc
     2101 tattttcttt tcgtttgctg ctttctcgcc ttgttccgcg aagattcggc gcacgtcctc
     2161 cactgtttcc cggtatgtgc cgttgctgat ggccgatacg atttcttctt ctgtcatttc
     2221 ggctatgacg cggtaaaagt cttgaaagat ggtaaaatgc tccatgttgg tttgttcttt
     2281 ttaatttgcg gttgcaaaga aatttgtttg taaaataata gaatggaact tttcgttttt
     2341 ttgattaagt tcctagggtt ctcccaaata gcgtatgatc gttgccactt gccggggaga
     2401 gagaaggcgg ttgcgggcac gatagccttc ttgttcgagt tcttgcagga gcggaaggtt
     2461 gtagacaatc caacgacgta gtatttgtaa ggctacttta aggcatacgt tggggttgta
     2521 aagatgagct agttccgact ttttataagt gcagatatga aacggctgat ccggataata
     2581 catgatgaat gatatttggt ttatgctata aagttactga aaattaatgg tttaataaaa
     2641 gaataacgtc attattattc atagagtatc aggaaacttc gttggggaat ccggagggtg
     2701 aaaaatgtgt tatctttatg tcgttaacaa ataaggaacg cgttcgaaat gtgtgaaatc
     2761 ttaaaattat tttattatgg caatgacagt aacttattca gtagtgccgc gcaagaatcc
     2821 tgccaaaaag gatgagtcgg caaagtatta cgcgcaggca caggcttcgg gagagttgga
     2881 ttttgaagag ttgtgcgagg gaatcaccag ccggtctacc tgtacggaga cagatgtgcg
     2941 tgccgccatt tcgggtattc tttatgaggc gaagcgtgcg ttgaaagcgg gaagaatagt
     3001 ccggttgggt gatttgggca gtttgcagat cgggttgaac agtgaaggag ctgtgtcggt
     3061 caaggagttt tcaagttcgc tgattacggc tgcccatatt atttttcgtc ccggaaagac
     3121 gttggcggac atcacgaaga ttcttagcta ccagcaggta acgacgcgtg cggtggccca
     3181 aacgggcggt agcggcaatg aaggagaaga tgatgacaaa ggttccggtg gcggttcaga
     3241 cggtggtgga agcggagaag ctccggatcc ggcagcatga taactttaat attgaatcaa
     3301 atgaaagaaa ttgtaactaa gattttggat gtaattatgt tccttgtgcc tttcttcgga
     3361 aaaagaaagc ggaacagaat tgtgagagag gtgcgcttca atgctaccca taaggaagtg
     3421 tgtaatgtga aaacgacgga aagggagaag gacgatgaga aagattagtc tgattgtgat
     3481 tcactgctct gctacccgtg ctgatcgcga cttcacggcg aaggatgtgg acactgccca
     3541 tcgctttcgg ggcttttctt gttgggggta tcattattat atccgcaagt cgggacagat
     3601 agagccgatg agggatgaag atacggtggg tgctcatgca cgcggtttta atgcgattag
     3661 tttaggagtg tgctatgaag gcgggctgga tgagaacgga aaggcagcag atacgcgtac
     3721 ttcccgccag aaagaggcga tgcatcgtct ggtaaacgag ttgttgcagc gttatccgga
     3781 tgcaaaggtg gtcggtcatc gggacttgag tcctgacacc aattataatg gaattgttga
     3841 cccttgggag cggatcaaag agtgtccctg tttcgaggtg aaagcagaga catggtgatt
     3901 tttatcctat ttattactgt ggtggttaat tgaccaccac agtagtgaaa tactctttaa
     3961 atacctccgc ggctttttca agttgttcgg gggtattttc ttttacgtct ttcagcttgt
     4021 attcctgctc catcgcttca tacttatgta cgcctaatgt atgataaggc aggatttcca
     4081 ctcgttgcac catcttatat tttcccagag cctctcccaa gcggcggata tcctcttcga
     4141 aatcgctgta tccgggcact aatacataac gcagccagaa aggtttctcg ttttcttcca
     4201 gccatgctgc cgtgcggatg gtttgctcgt tacttcttcc ggtcagtgct tgatggcgtg
     4261 ccggattgaa ctctttaata tccagcaata caagatccgt cagtttgaaa agttcttcta
     4321 cttcttcgtt ccagataccg ccgttgctgt ctatgcatac atgtattccc ttttctttca
     4381 gttcacgcac taaagggact agtgctttgg cctgaaacgt aggttctcct ccggagaaag
     4441 tgactcctcc gcgtttcccg aaaaaaggac gttggctcat agccatgcgg acgatttctt
     4501 ccggtggggt aggtgtacct ccttttccgg ctatcgtgtc gggattggca caatacaggc
     4561 agcggaaatt gcatccttga agaaaaacga cgagccgtaa gcccggcccg tcgaatgttc
     4621 ccatactttc gtatgaatgt acgtttatcg tcatattgag tagatctgat tacatacgtt
     4681 cgtggaagct acggctgata acttccaatt gatgttcgcg gctcaacttc acgaagttta
     4741 cggcataacc ggaaacacgg atggtgagtt gcggatattt ttccggatgt tccatggcgt
     4801 cgtaaagcat ttcacggttc agtacgttca cgttcaggtg gtgagcgcct ttggtgaagt
     4861 aaccatccat cattgtcacc agattttcta cgcgatcttc ttcagtagct cccagtgatt
     4921 tcggaacgat ggagaaggtg ttgctgatac cgtcctgtga gtcgcggtaa cgtaatttcg
     4981 ctacggaact caaagaggcg atggcaccgt tcttgtcacg tccgtgcata gggttggctc
     5041 ccggagcaaa agcgacacct ttggcgcggc cgtcgggagt agcacctgtc ttcttgccat
     5101 acatcacgtt ggaagtgatg gtgagcaaag acagagtagg acgggcattt ttgtaaaccg
     5161 gaagtttctt caattcttcg ctgaagaaat agaccaggtc aacacccagg tggtctacct
     5221 tgtcgttgtc attaccgaag caagggaatt cgccttgaat atcgaaacct tctgtcagac
     5281 cgatgtcgtt gcggcgtgcg gttactttgg catatttgat ggccgacagt gaatcgaggg
     5341 cgatagaaag tccggctaca ccgtaagcaa ggttgatgcg tgggttggta tctacaagag
     5401 ccatttgggc tttttcgtag taatacttat catgcatata atggatgatg ttcatcgctt
     5461 cgttgtaaac acgggcgatt tctgtcaata ccttcttgta gttgctcatt acttcttcga
     5521 agttcagtgt gtcgctggtt agtacgggga tgtttttcac cattaccgta ccggtgtttt
     5581 cgcaacgtcc gccgttgatg gcaagcagca gggctttagc caggttgcaa cgtgcgccga
     5641 agaactggat ttgttttccg atttcctgat aagatacgca gcacgcgatt ccgtagtcat
     5701 cagactggcg cacttcacgc atcaggtcgt cgttttcata ttggatggaa gaagtgtcga
     5761 tagatacttt tgcgcagaat tctttgaatc cttccggcag ttccgggctc cacaatacag
     5821 tcaggttcgg ttccggtgaa gggccgaggt tgtagagtgt ttgcaggaag cggaaagaag
     5881 tctttgtcac cttggtgcgt ccgtcgttga ggcgtccgcc cagggattcg gttacccatg
     5941 tcgggtcgcc ggcaaagatg tcgttgtaag attgcatacg caggtggcgc accatacgca
     6001 gtttgatgac aaactggtcg atcagctcct gtgcgaaaga ttctgtgata gttcctttgc
     6061 tcaattcata ttccatataa atgtcgagga acgaagagac gttacccagt gacatggcag
     6121 caccgtcttg ttcttttacg gcagcaaggt aagccatgta cacccattgt actgcttctt
     6181 gtgcggtgta ggcaggacgg ctcaagtcga gtccgtagta ttcgcccatt accttcatgt
     6241 ccttcaatgc cttgatttgc tctgccactt cttcgcgcag gcggatgcgg gcttcggtca
     6301 tcggaccggt aaggttacgc aaatcttctt tttttgcctc aatcagtcgg tcgataccat
     6361 agagagccat acggcggtag tcgccgatga tacgtccgcg ggcatagttg tcaggaagtc
     6421 cggtgaggaa tcccagagaa cggaacgaac ggatttcttc ggtatatacg tcaaatactc
     6481 cgtcattgtg cgtcttgcgg tagtgagtga atatatcttt tacacggtcg tccacttcca
     6541 cgccgttttc gtggcaggct ttgctcacca cgttgatacc tccgaaaggt ttgattgcac
     6601 gtttcagaag ttcgtctgtt tgcagaccga cgatcagttc gttttctttg tcgatatatc
     6661 cggctttatg agaagtgatg gttgagacgg tgacattatc cagtgagcgt actccgttat
     6721 tggctctttc ttctgccagt gcttcgaggc agcggttcca tacagcttta gtgcgttcgg
     6781 taggtccttc gaggaaagaa gcatctccat aatacggagt gatgttgtga ctaacgaaat
     6841 ctcttacgtt gatttctgtg ctccaaagac cgtctttaaa gatcttattt aattccataa
     6901 atgataaaat tagaggttgt agttatcctt ttctttagag cgcacaaagt tacaacgttt
     6961 tttcatataa tcagcataaa tatgctgtat tttatgcttt tcaacataaa atacctagat
     7021 atagctattc tttggaaatg atgttcgtga tttttttttg tgaaaactct tgcaagttta
     7081 aaatatattc gtacctttgc accgctttta acaaaaagca cttctgaaaa ggaagttttg
     7141 gagaggtggc agagtggtcg attgcggcgg tcttgaaaac cgttgtactg cgaggtaccc
     7201 ggggttcgaa
//
