LOCUS       c00282_gnlPro..         5872 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JELABEBK_282.
ACCESSION   c00282_gnlPro..
VERSION     c00282_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 14:38:00
            Original ID  :: gnl|Prokka|JELABEBK_282
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5872
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5872
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1196:5753]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1197..5753
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5872
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5872
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             2..1012
                     /locus_tag="ctg282_1"
                     /transl_table=11
                     /translation="NKMVNYKDLGLVNTREMFAKAIKGGYAIPAFNFNNMEQMQAIIKA
                     AVETKSPVILQVSKGARQYANATLLRYMAQGAVEYAKELGCAHPEIVLHLDHGDTFETC
                     KSCIDSGFSSVMIDGSHLPYEENVALTKKVVEYAHQFDVTVEGELGVLAGVEDEVSSDH
                     HTYTDPEEVIDFATRTGCDSLAISIGTSHGAYKFTPEQCHIDPKTGRMVPPPLAFEVLD
                     AVMEKLPGFPIVLHGSSSVPEEEVETINKYGGALKAAIGIPEEELRKAAKSAVCKINID
                     SDSRLAMTAAVRKVFAEKPAEFDPRKYLGPARDNMEKLYKHKIINVLGSDNKLAQ"
     CDS             complement(1197..2357)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_2"
                     /sec_met_domain="OAD_beta (E-value: 3.4e-154, bitscore:
                     501.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLVFIYLAIAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKNERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFVIATASGVIFVKIFNIFLKKGNKINPLIGNAGVSAVP
                     DSARISQVIGLEYDSTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(2359..2790)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             complement(2815..3735)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_4"
                     /sec_met_domain="OAD_gamma (E-value: 3.3e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQNNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGALGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             complement(3767..5320)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_5"
                     /sec_met_domain="CT_Btheta (E-value: 9.5e-291, bitscore:
                     953.0, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAQTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(5349..5753)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_6"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.6e-24, bitscore:
                     75.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAEGKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 aaacaaaatg gtaaattaca aagatttagg attggtaaac acaagagaaa tgtttgctaa
       61 ggctatcaaa ggtggatatg ctatcccagc attcaacttc aacaatatgg aacaaatgca
      121 ggctatcatc aaggctgctg ttgaaactaa atctcctgtg attcttcagg tttctaaagg
      181 tgctcgtcag tatgctaacg ctactttgtt gcgttacatg gcacagggtg ctgtagaata
      241 tgctaaagaa ttaggctgcg cacatccgga aatcgttctt caccttgacc acggagatac
      301 attcgaaact tgcaaatctt gtatcgattc aggtttctct tcagtgatga ttgacggttc
      361 tcatcttcct tacgaagaaa acgtagcatt gactaagaaa gttgttgaat acgctcacca
      421 gtttgacgta actgtagaag gtgaacttgg cgtattggct ggtgtagaag atgaagtttc
      481 ttccgaccat cacacatata ctgaccctga agaagtaatc gacttcgcta ctcgcactgg
      541 ttgcgactct ttggctatct caatcggtac ttctcacggt gcttataaat ttactccgga
      601 acaatgccac atcgacccga agactggccg tatggttcct cctccattgg ctttcgaagt
      661 attggacgct gtaatggaaa aacttccggg attccctatc gttcttcacg gatcatcttc
      721 agttccggaa gaagaagtgg aaactatcaa caaatatggt ggtgcactga aagctgctat
      781 cggcattccg gaagaagaat tgcgtaaagc tgctaagtct gctgtttgca aaatcaacat
      841 cgactctgac tctcgtctgg ctatgactgc tgctgtacgt aaggtattcg ctgaaaaacc
      901 ggctgaattc gacccacgta agtatcttgg cccggctcgt gacaatatgg aaaaactgta
      961 caagcacaaa atcatcaacg tgctcggttc tgacaacaaa ttggcacagt aataatctcc
     1021 ggattattcc ataaataaaa gaaagaggct gctccggttt gaacactggt agcagccttt
     1081 tttatgacgt ttaatcgttt cagaacggag agaagataac actatggctg taataaagaa
     1141 cacccgtgtc acaactgaat gtaacacggg tgtttttatc tattttataa tgtttcttac
     1201 atcaagaatc caagcaggat accggcagca acagctgaac cgatcactcc ggcaacgttc
     1261 ggacccatag cgtgcatcag caaatagttg gtcgaatcgt attccaaacc aatcacttgc
     1321 gagatacgtg cagagtcggg aacagcagaa acacctgcat taccgatcaa cggattgatc
     1381 ttattaccct tcttcaggaa gatattgaaa atcttaacga agatgacacc agatgccgtt
     1441 gcaataacaa atgacaatgc accgagggca aagatcttga tagagtcaag cgtcaggaat
     1501 tcggatgctt gtgtagaagc acctactgtc agacccaaca ggatagtgat tgtatcaatc
     1561 aacggaccac tggcagtatt agccaaacgg cgggttacac cactttcttt caacaggtta
     1621 ccgaagaaca gcatacccag caaaggcaga ccggaaggaa ccaggaaaca agtcagcaac
     1681 aaaccgatga tcgggaaaat caccttctcc gtgtgagaaa cggcacgtgg cggtttcatg
     1741 cggatgacac gttcgttctt ggtggtgagc agacgcatga taggcggctg tatcaccgga
     1801 accaacgcca tataggagta ggcggacacc gcaatggctc ccatcaggtt aggtgctagc
     1861 ttggaagaaa ggaagatggc cgtcgggccg tctgctccac caatgatacc gatagcaccg
     1921 gcttgcattg ggtcgaaacc catttccaat gcgatcatat atgcaccgaa gataccgaac
     1981 tgtgcagctg caccaatcag catcaatttt ggattagaga taagtgccga gaagtccgtc
     2041 atggcaccga tacccaaaaa gatgagcggc ggataccagc cggaggtcac tccctgatac
     2101 aatatattca atacagaacc ttcttcataa ataccgactt tcagcccggc atccatatta
     2161 aaaggtatat taccgatcaa tataccgaaa ccgataggaa tcagcagcat cggttcgaac
     2221 tctttggcta ttgccaaata gatgaatacc aagccaacga gaatcatgac tacatgccct
     2281 accgtagcat tggcaaagcc tgtgtatgtc cagaagtcgg ccaggttatt tcctaaaaag
     2341 tttataaagt ctcccatatt attcaataat tacgaggtca ttaccttcaa gaacagagtc
     2401 tcccttgttg acgttgatgg cagtaatctt accgtcctta tccgcattga tattgttttc
     2461 catcttcatg gcttccaata tgataatggt ctgtcctttc tttacagtat cgcctacatt
     2521 cactttgatg tcgaggatca cacccggcag cggagatttt actcctgatt ttccggtaga
     2581 cggagcggtc ggtttcacta cttgagtagg agcagcagga gcattaggca tcgggcgtac
     2641 tgtcacgggt ttcgcaacag gcttcggctg tttctccatt tctactttat agtgtgtacc
     2701 gttcacttct acatgagcga tgttgtcttc aatatctccg atggttactt tatatgagtt
     2761 accgttgatt ttatatttat attctttcat cgtttttact ttttaaaagg tgacttactt
     2821 tctgggagga gtttcacgca aagtgtaaat cttcgaactc catggtgagt agctgcgttt
     2881 tacgcgggtg atggtcagca ccgtttcttc tacgtcgtgc acctcatcct gcatttcgtg
     2941 taatgccata gaaatagcag cgtatacttc gcccggagcc tgtgacagtt ccttagcttc
     3001 atgcttgtca atgcctttag atttcatagc gttgcgcttg ctcaaattaa cagctacttt
     3061 tcctacaatc ttaaaagcaa taaagagaag aatcaatccg cagaatacta cgctcatggc
     3121 agaaatggac attccaacgc catcagcatc gtgttcttca aacttttcca tcttggaatt
     3181 gctgtcaaga gtcttgttgt tggtgctcgg agtcacgtat ttatcaccgc ttccactttt
     3241 tacttcccat tcagctgccc cgtcgcttac acgagcgtat gattggttgg gaccgagcgc
     3301 gcctgcgggc actacgatct ggtcgagcaa cttcttgccg gagtcgaaca gacctaccca
     3361 gttagctgtt tccggattca gcttgaagct ggtatggaaa gtaccacggt taggttcgcc
     3421 atctgcccag aatagtgcat gttggcgtgg tttcactaag gtaagtatat cacctttcgg
     3481 gataaaataa gtaactgtat cgcccggttg gctacttact ttcagcaagc aagctgccag
     3541 gtcggcgcta ccgaatgatt tattgaatat ttcaatccat gcgctgtgca atccgtagtc
     3601 atcttgaaaa ttattctgat tgtctatcag gatttcgttc agtaccagct tgttgttcga
     3661 cttttgctct ccacaagaag tcagcccaat cagcaacagc aaagaaagga atattccgat
     3721 tttggttttg ttcataatcg ttcttgtttt aatgtgaaaa gtaggattac aacggaatat
     3781 taccatgctt cttagccgga ttactcaatt tcttagtctg caactgctgc aaagcacgga
     3841 taacacggaa acgtgtgttg cgcggttcaa tcacatcgtc aatgtagcca tatttagctg
     3901 cattgtaagg attggcgaac aatttcgtgt attctgcttc tttttcagca aggaattgag
     3961 cgggattttc ctgatctttg gcttcgcgtg cgtacaatac ttctactgct cctgctccac
     4021 ccattactgc gatttcagca gtcggccatg cgtagttcat gtcaccgcga agttgcttac
     4081 agctcattac gatgtgagaa ccaccgtagg atttacgcaa tgtaatcgta accttaggta
     4141 cagtagcttc accgtaagca tacaatagct tagcaccatg aaggataacg ccgttgtatt
     4201 cctgacctgt tcccggaagg aatcccggta cgtctaccaa cgatacgata ggaatattga
     4261 atgcatcgca gaaacgaacg aaacgtgcac ccttacgaga tgcgttgctg tcgagcacac
     4321 cagccagata cttaggctga ttagcaacga tacctaccga ttggccgttg aagcgggcga
     4381 aaccgatgat gatattcttt gcataatctt tctggatttc aaggaattca ccgttgtcca
     4441 caatagcgcc gatcacttcg tacatgtcat acggtttagt agggctgtcg gggataatat
     4501 cgttcaaaga atcttccaga cggtcgattg ggtctgtgca atctacataa ggagcttctt
     4561 caaggttgtt ttgaggaata tagctcaaca gtttgcgaat cagttcgaaa ccttcttctt
     4621 ctgtttgggc ggtgaaatga gtcacacccg atttagtgga gtgaacgctt gcgccaccga
     4681 ggttttcctg gctaacgtct tcacctgtaa cggtcttcac cactttcggt ccggtgagga
     4741 acatataaga agtgccttcc atcatcagtg tgaagtcggt cagagccgga gaataaacag
     4801 caccaccggc acaaggaccg aagataccgg aaatctgcgg aataacacct gaagcaagga
     4861 tgttgcgttg gaagatttct gcataacctg ccaaagcgtt gataccttcc tggatacgtg
     4921 caccacccga gtcgttgata ccgatacagg gagcgcccat cttcatggct tgatccatga
     4981 ttttacaaat tttcaatgac atcgtttctg acagtgaacc ggcagaaaca gtgaagtctt
     5041 gtgcgaacac atataccaga cgtccttcga tcgtaccgca gccagttact acaccgtctc
     5101 ccgggtaatg ttttttctcc atgccgaagt tcgtgcatct gtgctcaacg aacatgtcca
     5161 tttcttcgaa gctaccctcg tcaagcagca tagccagacg ctcgcgtgct gtgtatttcc
     5221 ctttttcgtg ttgcttcgca attgcttttt caccgcctcc gatacgtgct acggcacggc
     5281 gttcaataag ctctttaatt ttttcaagtt gattactcat gagttcttgt tagttttaga
     5341 gtttatgatt aatgttcgca gagttctgtt aacacaccca aagttgattt cgggtgaagg
     5401 aaagcgatat tcaaaccttc agcacctttg cggggagctt tgtcgataag gcggatttct
     5461 ttgccttctg cctctgccag cgcattggct acgccatctt ctacagcaaa tgcgacgtgg
     5521 tgaacgcctg cacctttgtt ttcaatgaat ttggcaatag tactctccgg gcaagtaggc
     5581 tccaacagtt caatttttgt ttcgcccact tttaaaaaag cagttcttac tttctgatct
     5641 tccactgttt caatgttgta acacttcaga cctaatacat tttcgtagta aggaagggct
     5701 tcttcgatgc tttttacagc aatgcccaga tgttcaatgt gtgaaatctt cataactata
     5761 ctttttttat ttggttttaa atctgtgctt ataaggcata aaacagcaca aagaaagaca
     5821 tttcttatct aataaagaaa tatttctgca tttaattatt ggattattaa ac
//
