LOCUS       c00066_gnlPro..         6263 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CBKGKHGP_66.
ACCESSION   c00066_gnlPro..
VERSION     c00066_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 14:54:38
            Original ID  :: gnl|Prokka|CBKGKHGP_66
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6263
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..663)
                     /locus_tag="ctg66_1"
                     /transl_table=11
                     /translation="MRCNAFVEAWKNHHRRASMILGLSLLSAPLSYSVYAEDGVSNTMV
                     QVVTQTKTVKGTIIDESGEPLIGVSIVVKGTSTGTITDFNGNFSIDLPAGRKELVISYI
                     GYKEQTVTVTGNGPVNVKMVSDTQALDEVVVVGYGTMKKRDLTGAITSVKSEDVVMNPS
                     SNPMQALQGKVAGLDITRESGQAGSGVKMQLRGNRSFQKDSGNPLFIIDGMPGDYSTL"
     protocluster    1..6263
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2076:6261]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2077..6261
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6263
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6263
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             903..1901
                     /locus_tag="ctg66_2"
                     /transl_table=11
                     /translation="MPLKLTTYYHGKDIPELPGKNAFHSKELFQIYEATPGYTPLLIVA
                     TEDGRPMARLLAAIRKAKKWLPSSLVKHCVVYSEGEFLDESLSTNKEKAEEVFGDMLEH
                     LTQEASRSCVLIEFRNLNNSMFGYRVFRANDYFPVNWLRVRNSLHSMKKTEDRFSPSRI
                     RQIKKGLKNGAKVEEAHTVEEIHDFSRMLHKVYSSRIRRYFPANDFFRHMNNMLIKGQQ
                     AKIFVVKYKEKIIGGSVCIYSGDDAYLWFSGGMRKTYALQYPGVLAVWKALEDAHQRGF
                     RHMEFMDVGLPFRKHGYRDFVLRFGGKQSSTRRWFRINWSWLNKLLVKFYV"
     CDS             2077..2481
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg66_3"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.8e-24, bitscore:
                     75.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAEGKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             2510..4063
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg66_4"
                     /sec_met_domain="CT_Btheta (E-value: 1.1e-290, bitscore:
                     952.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             4095..5015
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg66_5"
                     /sec_met_domain="OAD_gamma (E-value: 3.9e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQSNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGTLGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             5040..5471
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg66_6"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.3e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             5473..6261
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg66_7"
                     /sec_met_domain="OAD_beta (E-value: 2.1e-102, bitscore:
                     331.5, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLVFIYLAIAK
                     EFEPMLLIPIGFGMLIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKNERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTAS"
ORIGIN
        1 caatgtagaa taatcccccg gcataccatc aattataaat aaaggatttc cggaatcctt
       61 ctggaatgac cggttaccac gcaactgcat ctttactccc gaaccggcct gtcccgattc
      121 acgcgtaata tccaatcctg ccaccttacc ttgtaaagcc tgcatcggat ttgaacttgg
      181 attcatcact acatcttcac tttttacaga agtgatagct cctgtcaaat cgcgtttttt
      241 catcgtaccg taacccacaa caaccacttc atccaatgcc tgtgtgtccg aaaccatttt
      301 cacattgacc ggtccgttgc ctgtcactgt caccgtttgt tctttgtaac cgatataaga
      361 aatgaccagt tcttttcttc ccgccggaag atcgatagag aagttaccgt taaaatcggt
      421 aatcgtacct gtacttgttc ctttcacaac gatagaaact ccaatcagcg gctccccgct
      481 ttcatcaatg atagttcctt tcacagtttt cgtttgtgtg acaacttgca ccatagtatt
      541 agaaactcca tcttctgcat acacagaata tgacagaggg gcagacaata aagacagccc
      601 taaaatcatg gaagcgcgtc tatgatgatt cttccatgct tcaacaaatg cattacatct
      661 cattcgttct aatttaaagt taatattaat tctcgttgtt ttgcaaaaac tattcgatta
      721 taatcttaat taaagacgaa tctaatttta atacgtactc acttttcata ccatatttct
      781 cctataaaat atattttctc cattaaatac gcaactatat attatacttt acttcaagca
      841 gctccaacaa attctttgtt ttgttgttga ataaagaata aaaagtgtaa atttgtacct
      901 agatgccact taaactaacc acatattatc atgggaagga cattcctgaa ttaccaggaa
      961 aaaacgcctt ccattccaaa gagttatttc agatttatga agcgactccg ggctatactc
     1021 ctttattaat agtggcaaca gaagacggca gacctatggc acgcctgctt gccgccatcc
     1081 gcaaagcgaa aaagtggctt ccctcctctt tagtgaaaca ttgcgtagta tatagcgaag
     1141 gtgaatttct ggatgagtcg ctttcgacca acaaagaaaa ggcagaagaa gtctttggcg
     1201 atatgctcga acatctcaca caagaagcat cgcgtagctg tgtcctgatc gaattccgga
     1261 acctcaataa ttcgatgttc ggttaccggg ttttccgtgc caatgattat ttccctgtca
     1321 actggctgcg ggtacgcaat tcgctgcaca gtatgaaaaa gacggaagac cggttcagcc
     1381 cgtcgcgcat ccgtcagata aaaaaaggac tcaagaatgg agccaaagta gaggaggcac
     1441 ataccgtgga agaaatacac gatttctcac ggatgctgca caaggtttat tcctcccgca
     1501 tacgcagata tttccctgcc aacgactttt tccgccacat gaacaatatg ttaatcaaag
     1561 gacaacaggc aaaaatattc gttgtgaaat acaaagagaa aatcattggc ggttctgtct
     1621 gtatctattc gggagacgac gcataccttt ggttttccgg aggaatgaga aagacatacg
     1681 cacttcaata tccgggtgtg ctagccgttt ggaaagcatt ggaagacgca caccaacggg
     1741 gattccgaca catggaattt atggatgtag gacttccgtt ccgtaaacac ggttatcgtg
     1801 atttcgttct tcgcttcgga ggaaaacaaa gcagtacccg ccgttggttc cgcatcaatt
     1861 ggagttggct gaacaaactc ttagtcaagt tttatgtcta aatgtaatct caagattaca
     1921 aaacaatgtt ttatgctcct ttcccaccat tttttctgtt taataatcca ataattaaat
     1981 gcagaaatat ttctttatta gataagaaat gtctttcttt gtgctgtttt atgccttata
     2041 agcacagatt taaaaccaaa taaaaaaagt atagttatga agatttcaca cattgaacat
     2101 ctgggcattg ctgtaaaaag catcgaagaa gcccttcctt actacgaaaa tgtattaggt
     2161 ctgaagtgtt acaacattga aacagtggaa gatcagaaag taagaactgc ttttttaaaa
     2221 gtgggcgaaa caaaaattga actgttggag cctacttgcc cggagagtac tattgccaaa
     2281 ttcattgaaa acaaaggtgc aggcgttcac cacgtcgcat ttgctgtaga agatggcgta
     2341 gccaatgcgc tggcagaggc agaaggcaaa gaaatccgcc ttatcgacaa agctccccgc
     2401 aaaggtgctg aaggtttgaa tatcgctttc cttcacccga aatcaacttt gggtgtgtta
     2461 acagaactct gcgaacatta atcataaact ctaaaaccaa caagaactca tgagtaatca
     2521 acttgaaaaa attaaagagc ttattgaacg ccgtgccgta gcacgtatcg gaggcggtga
     2581 aaaagcaatt gcgaagcaac acgaaaaagg gaaatacaca gcacgcgagc gtctggctat
     2641 gctgcttgac gagggtagct tcgaagaaat ggacatgttc gttgagcaca gatgcacgaa
     2701 cttcggcatg gagaaaaaac attacccggg agacggtgta gtaaccggct gcggtacgat
     2761 cgaaggacgt ctggtatatg tgttcgcaca agacttcact gtttctgccg gttcactgtc
     2821 agaaacgatg tcattgaaaa tttgtaaaat catggatcaa gccatgaaga tgggcgctcc
     2881 ctgtatcggt atcaacgact cgggtggtgc acgtattcag gaaggtatca acgctttggc
     2941 aggttatgca gaaatcttcc aacgcaatat ccttgcttcc ggtgttatcc cacagatttc
     3001 aggtatcttc ggtccttgtg ccggcggtgc tgtttattct ccggctctga ccgacttcac
     3061 gctaatgatg gaaggtactt cttatatgtt ccttaccgga ccaaaagttg tgaagaccgt
     3121 aacaggtgaa gacgttagcc aggaaaacct cggtggcgca agcgttcact ccactaaatc
     3181 aggtgtgact catttcaccg ctaaaacaga agaagaaggt ttcgagctga ttcgtaaact
     3241 gttgagttat attcctcaaa acaacctcga agaagctcct tatgtagatt gcacagaccc
     3301 aatcgaccgt ctggaagatt ctttgaacga tattatcccc gacagcccta ctaaaccgta
     3361 tgacatgtac gaagtgatcg gcgctattgt ggacaacggt gaattccttg aaatccagaa
     3421 agattatgca aagaatatca tcatcggttt cgcccgtttc aacggacaat cggtaggtat
     3481 cgttgctaat cagcctaagt atctggctgg tgtgctcgat agcaacgcat ctcgtaaggg
     3541 tgcacgtttc gttcgtttct gcgatgcatt caatattcct atcgtatcgt tggtagacgt
     3601 accgggattc cttccgggaa caggtcagga atacaatggc gttatccttc atggtgctaa
     3661 actgttgtat gcttacggtg aagctactgt gcctaaggtt acgattacat tgcgtaaatc
     3721 ctacggtggt tctcacatcg taatgagctg taagcaactt cgcggtgaca tgaactacgc
     3781 atggccgact gctgaaatcg cagtaatggg tggagcagga gcagtagaag tattgtacgc
     3841 acgcgaagcc aaagatcagg aaaaccctgc tcaattcctt gcagagaaag aagcagaata
     3901 cacgaaattg ttcgccaatc cttacaatgc agctaaatat ggctacatcg acgatgtgat
     3961 tgaaccgcgc aacacacgtt tccgtgtgat ccgtgctttg cagcagttgc agactaagaa
     4021 attgagcaat ccggctaaga agcatggtaa tattccgttg taatcctact tttcacatta
     4081 aaacaagaac gattatgaac aaaaccaaaa tcggaatatt cctttctttg ctgttgctga
     4141 ttggtctgac ttcttgtgga gagcaaaagt cgaacaataa gctggtgcta aacgaaatcc
     4201 tgatagacaa tcagagtaat tttcaggacg attacggatt gcacagcgca tggattgaaa
     4261 tattcaataa atcattcggt agcgccgacc tggcagcttg cttgctgaaa gtaagcagcc
     4321 aaccgggcga tacagttact tattttatcc cgaaaggtga tatacttacc ttagtgaaac
     4381 cccgccaaca tgcactattc tgggcagatg gcgaacctaa ccgtggtact ttccatacca
     4441 gcttcaagct gaatccggaa acagctaatt gggttggtct gttcgactcc ggcaagaagt
     4501 tgctcgacca gattgtagtg cccgcaggca ctctcggtcc caatcaatca tacgctcgtg
     4561 taagcgacgg ggcagctgaa tgggaagtaa aaagtggaag cggtgacaaa tacgtgactc
     4621 cgagcaccaa caacaagact cttgacagca attccaagat ggaaaagttt gaagaacacg
     4681 atgctgatgg cgttggaatg tccatttctg ccatgagcgt agtattctgc ggattgattc
     4741 ttctctttat tgctttcaag attgtaggaa aagtagctgt taatttgagc aagcgcaacg
     4801 ctatgaaatc taaaggaatt gacaagcatg aagctaagga actgtcacag gctccgggcg
     4861 aagtatacgc tgctatttct atggcattac acgaaatgca ggatgaggtg cacgacgtag
     4921 aagaaacggt gctgaccatc acccgcgtaa aacgcagcta ctcaccatgg agttcgaaga
     4981 tttacacttt gcgtgaaact cctcccagaa agtaagtcac cttttaaaaa gtaaaaacga
     5041 tgaaagaata taaatataaa atcaacggta actcatataa agtaaccatc ggagatattg
     5101 aagacaacat cgctcatgta gaagtgaacg gtacacacta taaagtagaa atggagaaac
     5161 agccgaagcc tgttgcgaaa cccgtgacag tacgcccgat gcctaatgct cctgctgctc
     5221 ctactcaagt agtgaaaccg accgctccgt ctaccggaaa atcaggagta aaatctccgc
     5281 tgccgggtgt gatcctcgac atcaaagtga atgtaggcga tactgtaaag aaaggacaga
     5341 ccattatcat attggaagcc atgaagatgg aaaacaatat caatgcggat aaggacggta
     5401 agattactgc catcaacgtc aacaagggag actctgttct tgaaggtaat gacctcgtaa
     5461 ttattgaata atatgggaga ctttataaac tttttaggaa ataacctggc cgacttctgg
     5521 acatacacag gctttgccaa tgctacggta gggcatgtag tcatgattct cgttggcttg
     5581 gtattcatct atttggcaat agccaaagag ttcgaaccaa tgttgctgat tcctatcggt
     5641 ttcggtatgt tgatcggtaa tatacctttt aatatggatg ccgggctgaa agtcggtatt
     5701 tatgaagaag gttctgtatt gaatatattg tatcagggag tgacctccgg ctggtatccg
     5761 ccactcatct ttttgggtat cggtgccatg acggacttct cggcacttat ctctaatcca
     5821 aaattgatgc taattggtgc agctgcacag ttcggtatct tcggtgcata tatgatcgcg
     5881 ttagaaatgg gcttcgatcc gatgcaagcc ggtgctatcg gtatcattgg tggagcagac
     5941 ggcccgacgg ccatcttcct ttcttccaag ctagcaccta acctgatggg agccattgcg
     6001 gtgtccgcct actcctatat ggcgttagtt ccggtgatac agccgcctat catgcgtctg
     6061 ctcaccacca agaacgaacg tgtcatccgc atgaaaccgc cacgtgccgt ttctcacacg
     6121 gagaaggtga ttttcccgat catcggtttg ttgctgactt gtttcctggt tccttccggt
     6181 ctgcctttgc tgggtatgct gttcttcggt aacctgttga aagaaagtgg tgtaacccgc
     6241 cgtttggcta atacagccag tgg
//
