LOCUS       c00258_gnlPro..         3809 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CBKGKHGP_258.
ACCESSION   c00258_gnlPro..
VERSION     c00258_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 14:54:38
            Original ID  :: gnl|Prokka|CBKGKHGP_258
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3809
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3809
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2998]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2998
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3809
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3809
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..523
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.1e-49, bitscore:
                     156.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DVGQNQMISARYFKYTKERSIITSGGLGTMGFGLPAAIGATFGAP
                     ERTVCVFMGDGGLQMNIQELGTIMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTP
                     MLNPDYMKVASAYDIPSKRVFTREELKEAITEMLATDGPFLLEACVVKEGNVLPMTPPG
                     GSVNQMLLEC"
     CDS             581..1138
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_2"
                     /sec_met_domain="ALS_ss_C (E-value: 1.8e-19, bitscore:
                     58.3, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDIIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQENP
                     EASKLIRRYNARIVEVNPIFSIVEKNGMSEDITSLYGELKALNCVLQFVRSGRVAITTS
                     CFERVNEFLDGREAMYNQSKNQ"
     CDS             1144..1887
                     /locus_tag="ctg258_3"
                     /transl_table=11
                     /translation="MSEENRIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHANDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYENFSVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVDYVCDEPCPIEKPSRIKVTSDQPIATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYKTKRIRRFEMAYVAESYFGDELSFFCDEV
                     NANEFHVEVKKNGNEVVCRSKVIFE"
     CDS             1955..2998
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_4"
                     /sec_met_domain="IlvN (E-value: 3.4e-47, bitscore: 148.5,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 3.3e-42, bitscore: 132.7,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTTETVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQREGKTYDKAVADGWVPGETLFGIEEACEKGTIVMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWNDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGKAYERTIALGIGIGSGYLFETTFQREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHSPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYNSVKTGNEAQISIDSNSQPDYREKLNEELRQLRESEMWQTAVTVR
                     KLRPENN"
     CDS             3189..3809
                     /locus_tag="ctg258_5"
                     /transl_table=11
                     /translation="MMCFNLPQRMINIAKLACVISVVSVAFSSCLQKDVYDPANSGDKE
                     EVTLDNYFDFATTKNIQLNIDYGKECPRAYFEVYAENPLSYVAEGGQIIKKAGVSHIAT
                     GFTDIQGRYIKPASFPTAVSEVYIYSPDFGVPTLYKTKVVGSDVSAKITFENALDVTPV
                     DSSTRSAQTRAAVKFITDVIPNVLGTWNANSGKPDYLDASKKIN"
ORIGIN
        1 agatgtaggt caaaatcaga tgatatctgc acgttacttt aaatatacga aagaacgcag
       61 tatcattact tccggaggac ttggaacaat gggcttcggg cttcccgcag ctatcggggc
      121 aactttcgga gcaccggaac gtaccgtatg cgtatttatg ggagatggcg gactgcaaat
      181 gaatattcag gaactgggta cgattatgga gcagaaggct ccggtgaaga ttatctgcct
      241 gaacaataat tacctcggaa acgtacgtca gtggcaagct atgtttttca accgtcgcta
      301 ttcgtttacc ccgatgttga atccggatta tatgaaagtg gcctcggctt atgatattcc
      361 ttccaagcgt gttttcactc gtgaagaact gaaggaagct attactgaaa tgctggcaac
      421 ggacggaccg ttcctgctcg aagcttgcgt ggtgaaagaa ggcaatgttt tgccgatgac
      481 tcctccgggt ggttcggtaa accagatgtt gctggagtgc taggagaagt tgagagttga
      541 gaatggagag ttgagagttc gcatttaaaa aattagagtt atgagtgata aaacgttata
      601 tacaatcatt gttcattcgg agaatatagc cgggttgctg aatcaggtaa ctgctgtatt
      661 tacccgtcgg caaataaata ttgagagcct gaatgtatct gcttcttcta ttaaaggtgt
      721 acataagtat acaataacag cttggactga taaggacatc attgagaagg tagtgaaaca
      781 gatcgaaaag aagatcgacg tgattcaggc acattatttc acagaggatg agatttattt
      841 ccacgagatt gcattatata aggtgtctac tcctgctttt caggagaatc cggaagcatc
      901 caaactgatt cgtagataca atgcacgtat agtggaagtc aatccgatat tttctattgt
      961 cgagaaaaat ggaatgagcg aagatataac ttcgctctat ggtgaactga aggctttgaa
     1021 ttgtgttttg cagtttgtcc gttcgggacg tgtagcgatt actaccagct gctttgaacg
     1081 ggtgaatgag tttctcgatg gacgggaagc tatgtataac caaagtaaaa accaataaga
     1141 ataatgagtg aagaaaatag aatagggact tatcagtttg tggcagaacc gttccatgtt
     1201 gacttcaacg gacgtctgac tatgggggta ctgggaaatc atctgctgaa ttgtgcaggt
     1261 tttcacgcca atgaccgtgg ttttggtatt gcaacgctga atgaggataa ttacacatgg
     1321 gtgctttcac gtctggctat tgagttggat gaaatgcctt atcagtatga gaatttctcg
     1381 gttcagacgt gggtggaaaa tgtctaccgt ttgtttacgg accgtaattt tgctattatt
     1441 gataaggatg ggaagaagat tggctacgca cgttccgtat gggcaatgat taatcttaat
     1501 acccgcaagc ctgccgactt gttgactttg catgggggaa gtatcgtaga ctatgtctgt
     1561 gatgagcctt gcccgattga aaagccttca cgcatcaaag tgacaagcga tcagccgata
     1621 gctaccctga cggcaaaata cagtgatata gatatcaacg gtcatgtaaa cagcatccgt
     1681 tacatcgagc atatccttga tttgttcccg attgaattgt ataagaccaa acgtatccgt
     1741 cgttttgaaa tggcgtatgt ggcggaaagc tattttggtg atgaactctc tttcttctgc
     1801 gatgaagtga acgcaaacga gtttcacgta gaagtgaaga agaatggcaa cgaagttgtt
     1861 tgccgctcga aagtgatatt tgaataaatg tgagaatata aaatcaatta ataacagatg
     1921 gcgtaagtca tcaaaaacta aaaacaaaaa gaaaatggca cagttgaatt ttggaggtac
     1981 tactgaaact gtagtaatcc gtgacgaatt tccattggaa aaagctcgtg aagtattgaa
     2041 aaatgaaaca atcgctgtaa tcggttatgg cgtacagggt cctggacagg cactgaactt
     2101 gcgtgataac ggtttcaatg taatcgttgg tcaacgtgaa ggaaaaacat acgataaggc
     2161 agtagcagac ggatgggttc cgggtgaaac tttgttcggt atcgaagaag cttgtgagaa
     2221 aggtactatt gtaatgtgtc tgttgtctga cgcagcagta atgtcagtat ggcctactat
     2281 caagccttac ctgactgccg gaaaagctct ttatttctca cacggttttg ctatcacttg
     2341 gaacgaccgt acaggtgtag ttcctcctgc tgatatcgac gttatcatgg ttgctcctaa
     2401 aggttccggt acatctttgc gtactatgtt cctcgaagga cgtggcttga actcttctta
     2461 tgctatctat caggatgcta caggcaaggc ttacgaaaga accatcgcac tgggtattgg
     2521 tatcggttcc ggttatctgt tcgaaacaac tttccaacgt gaggctactt ctgacctgac
     2581 aggtgaacgt ggttcattga tgggggctat ccaaggtttg ttgctggcac agtacgaagt
     2641 attgcgtgaa aacggtcata gtccttctga agcattcaat gaaacagtgg aagaactgac
     2701 acagtcattg atgccgttgt tcgcaaagaa cggtatggac tggatgtacg ctaactgctc
     2761 tactactgct caacgtggtg ctctcgattg gatgggccca ttccacgatg ctatcaaacc
     2821 ggtagttgaa aagttgtata acagtgtgaa gactggtaac gaagcacaga tttctatcga
     2881 cagtaattca caacctgatt atcgtgaaaa gttaaacgag gagctccgtc aattacgcga
     2941 aagcgaaatg tggcagactg ctgtaactgt tcgtaaactt cgtccggaga ataattaatt
     3001 agaccagatt atacaagttt taaaagaagg agagggcttt ttgctttctc cttttttatt
     3061 aataagatat ttaaaaatgg gtaggtgaag cttgcagaaa cttttttttg aaaaatatat
     3121 caataatgtg attaaaatat gcaagtttat gtttatattt gcactatcaa ataaaatgaa
     3181 cgagaagtat gatgtgtttt aacttacccc agagaatgat taacattgct aaattagcat
     3241 gtgttatcag tgttgtttct gttgctttct caagctgttt acagaaagat gtatatgatc
     3301 ctgctaattc aggagataaa gaagaagtta ctttagataa ctatttcgac tttgccacta
     3361 ctaaaaatat tcaattgaat attgattatg gaaaggaatg tcctagggct tattttgaag
     3421 tttatgctga aaatccgctt tcttatgtgg cagaaggcgg tcagattata aagaaagcag
     3481 gggtgtcaca cattgcaacc ggatttacag atatacaagg acgttatatc aaaccggctt
     3541 ctttcccgac agcagtttca gaagtttata tttattctcc ggattttggt gtgccgactt
     3601 tgtataaaac taaagttgtc ggttcggatg tcagcgcaaa aataaccttt gagaatgctc
     3661 ttgatgttac tcctgtggat tcatcaacga gaagtgcaca aacaagggct gcggtgaaat
     3721 ttattacaga tgtaatcccg aacgtattag gaacttggaa tgcaaattca ggtaagcctg
     3781 actatttgga tgcttctaag aagatcaac
//
