GCA_029537295.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaPHKLFAIJ_1 (original name was: gnl|Prokka|PHKLFAIJ_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	T3PKS	547,822	588,991
Region 1.2	NRPS-like,NRPS	1,833,794	1,893,475
Region 1.3	RiPP-like	2,057,792	2,069,051	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%
Region 1.4	cyclic-lactone-autoinducer	2,458,740	2,479,444	auriculamide	NRP	9%
Region 1.5	terpene	2,744,842	2,765,723

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	T3PKS	547,822	588,991
Region 1.2	NRPS-like,NRPS	1,833,794	1,893,475
Region 1.3	RiPP-like	2,057,792	2,069,051	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%
Region 1.4	cyclic-lactone-autoinducer	2,458,740	2,479,444	auriculamide	NRP	9%
Region 1.5	terpene	2,744,842	2,765,723

Compact view

gnlProkkaPHKLFAIJ_1 - Region 1 - T3PKS

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Location: 547,822 - 588,991 nt. (total: 41,170 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaPHKLFAIJ_1 - Region 2 - NRPS,NRPS-like

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Location: 1,833,794 - 1,893,475 nt. (total: 59,682 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 1833793 - 1893475:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala) + (D-Ser - Gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 1833793 - 1875632:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala) + (D-Ser - Gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 1837606 - 1893475:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala) + (D-Ser - Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_1912

AMP-binding (11..190): X

ctg1_1915

Search NORINE for peptide: strict or relaxed

AMP-binding (575..961): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1981..2359): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DITQMGIVWK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

ctg1_1916

Search NORINE for peptide: strict or relaxed

AMP-binding (8..381): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVTQAAVAGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1000..1372): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSHGYGCVLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1967..2333): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLIVAAVIK
Nearest Stachelhaus code(s):: Ala DVWVVAAVIK (68% 8Å match); S-bohPhe or Phe DALIVAAVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaPHKLFAIJ_1 - Region 3 - RiPP-like

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Location: 2,057,792 - 2,069,051 nt. (total: 11,260 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaPHKLFAIJ_1 - Region 4 - cyclic-lactone-autoinducer

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Location: 2,458,740 - 2,479,444 nt. (total: 20,705 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

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transport-related genes

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resistance

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaPHKLFAIJ_1 - Region 5 - terpene

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Location: 2,744,842 - 2,765,723 nt. (total: 20,882 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.