LOCUS       c00259_gnlPro..         5153 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BLHPFIGP_259.
ACCESSION   c00259_gnlPro..
VERSION     c00259_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 20:22:36
            Original ID  :: gnl|Prokka|BLHPFIGP_259
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5153
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5153
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[229:5087]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      230..5087
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..5153
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5153
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(3..185)
                     /locus_tag="ctg259_1"
                     /transl_table=11
                     /translation="MVKERFYKTEVVPRCLTFKQPAGTSRGVYTTRKLWEVRIRKEDEP
                     SVIGIGECAPLPDLSC"
     CDS             complement(230..1054)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_2"
                     /sec_met_domain="ECH_1 (E-value: 1.3e-82, bitscore: 265.2,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METKREWTTIKEYEEILFDFYNGIARITINRERYRNAFTPTTTTE
                     MSDAFYICRERPDINVVVLTGAGDTAFCAGGDMNVKGRGGYVGKDGVPRLNVLDVQKQI
                     RSLPKPVIAAVNGFAIGGGHVLHVVCDLTIASENAIFGQTGPRVGSFDAGFGSSYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLDKLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATMLYYMTDEAQEGGKAFLEKRRPRFEDYPKFP"
     CDS             complement(1058..2746)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.6e-08, bitscore:
                     21.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFN
                     CHSVTDERSAGFFAVGLALHGGKPAAVCCTSGTALLNIHPAVAEAFYQKVPLVVISADR
                     PAAWIGQMDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEALLELNHHGKGP
                     VHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMS
                     LIYLFEKKICKLLYKHFTWLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVIT
                     YGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLTTVIEMDAFEFLEKIAYMLEN
                     RQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVP
                     ETVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRI
                     LLLNNGGGEIFQALPGFKMNERTHRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAM
                     SAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQNI"
     CDS             complement(2761..3858)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg259_4"
                     /sec_met_domain="Chorismate_bind (E-value: 5.8e-55,
                     bitscore: 175.0, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPRDSYEIIDTLIKQGRSFAIWRIPGEDRLHFRMQSAGSPCLLY
                     DIKDLNERSGFVIAPFQVTAERPIVLIQPDCFEIPSESDWDFSREKNEFPSNEIEIPSE
                     SEEKERYAYHFSLFTSPLLKGSQDKLVLSRSKTIRKDPSFSPGKAFFAAVERYIRSYVY
                     LCHTPETGTWMGGTPEILLSGEKGDWQTVALAGTQSLRDGKLPKSWDHKNWREQQLVAS
                     YIRRQLSTLGITPEEKGPYSARAGEVSHLKSDFFFSLPNPEKLGDVLQLLHPTPAVCGL
                     PKEEAYHFIIENEGYDRSYYSGFIGWLDPKGKTDLYVNLRCMNILPQTFTLYAGGGLLA
                     ASQLEDEWQETEDKLDTMRRIVGNL"
     CDS             complement(3855..5087)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_5"
                     /sec_met_domain="HBT (E-value: 7.1e-18, bitscore: 53.4,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDKKEITPRTHAALLKAQQMGVHVVLASGR
                     PTNGVQPLAEALELNHYGGFILSYNGGQIINAQTGELMFEKRIDPAMIPYLNRKAKENG
                     FAIFTYHKDYILTDSPENKHVQEEAELNKMRIIGVENFPEAVDFAPCKCILTSDDENNL
                     VGLENHWKKRLDGVLEAFRSEDYFLEVAPHFINKGNTLAVLMEMLNITTEEVVAIGDGV
                     ADVSMLQLAGTGVAMGNARDSVKACADFTTLSNNMDGVAVAIEKAILATIKPTEVPLDQ
                     LNARAKHALMGNLGIQYTYASEDRVEATMPVDERTRQPFGILHGGATLALAETVAGLGS
                     MILAKPDEMVVGMQVSGSHMSSAHEGDTVRAVGTIIHKGRSSHVWNVDIFTSTDKLVSS
                     VRVINSILKKK"
ORIGIN
        1 tcgcaactga gatccggcag gggggcacat tctccgatac caataaccga gggttcgtct
       61 tccttgcgga ttcggacctc ccacaatttc cgggtcgtgt aaacaccccg tgaggttcct
      121 gccggttgct tgaaagtcaa gcaccgggga accacctctg tcttataaaa cctctccttc
      181 accattcatt ttcaattttc aattttcaat tctcaattct caattccctt tacgggaact
      241 tcgggtaatc ctcgaagcgt ggacggcgtt tctccaagaa tgccttgccg ccttcctgcg
      301 cctcgtccgt catatagtaa agcatggtag cgtctccggc caactcctgt ataccggcct
      361 gtccgtccaa ctcggcgttc aagcctgcct tgatcatgcg caaggccaac gggctaagca
      421 acatcatctc ctcggcccat tgaacgtact cgtcctctaa tttatccaag gggactacct
      481 tatttaccag acccatatcc aaagcctcct gtgcattgta cttgcggcat aggaaccaga
      541 tctcacgggc ctttttttgt ccgaccacac gggcgaggta agaagagccg aacccggcgt
      601 cgaagctacc cacacgaggg cctgtctgtc cgaagatagc gttctcggaa gcgatcgtaa
      661 ggtcacaaac cacgtgcaat acgtgtccgc cgccgatagc gaaaccgttc acggctgcga
      721 tgacgggttt cgggagagag cggatctgct tttgtacgtc caatacgttc aagcgaggta
      781 cgccgtcttt acccacgtag ccgccacgtc ctttcacgtt catatcgcct cccgcgcaaa
      841 acgccgtatc tccggctcct gtcagaacga ctacgttaat gtccggacgt tcccggcaga
      901 tgtaaaaggc gtcgctcatc tccgtggtcg tagtcggggt aaaagcgttg cgataccgtt
      961 cccggtttat cgtaatgcgg gcgatgccgt tgtagaaatc aaataagatc tcctcgtatt
     1021 ccttgatggt tgtccattct cttttcgttt ccatatatta tatgttttgt gcttgggatg
     1081 aattttttag ttgatgataa tattccttga gcaatcgtac gtcttccgct ttgtccgtga
     1141 aaacctccat gaatagtggg tgttcgctcg gttgctccgt ttgggtaaag gcgctcatcg
     1201 ccgctgccaa ctcctcggca ttatggacag cggagtagga gaatcccctt tccgtggccc
     1261 atccctccgc tgaggtctgg tgggaggcgg taacgtaacg gtgcgtacgc tcgttcatct
     1321 tgaatcccgg caaggcttgg aagatctcgc cacctccgtt gttcaagagc aggatacgaa
     1381 ggttattgtg caagttaccg ttccacaacg cattcatatc atagaagaaa ctaagatcgc
     1441 ctataacgat aaagtttagt ttttgcgagg ccgaggcgta tccgatggcc gtagacaagg
     1501 agccttcgat cccgctcgta cctcggttac aacaaacctc aaccgtctcg ggtacggtgt
     1561 acaattgtgc gtaacgtacg gtggagctat tccccagatg caaggccgat tcagccggaa
     1621 gcgaacgtat caaaccaccg atcgccgcca tttcggagta agcgaactcc ggctccggca
     1681 gattccggga tttatattcc caagccttcg gatattcaat ctgcctattc tccagcatat
     1741 aagcgatttt ctccagaaac tcgaaagcgt ccatctcgat caccgtggtc aggctaccga
     1801 ataaatccgc tacctcccca tccaatgaga catgccaatg ctccttaggc gggtgtttcc
     1861 gcaaaaactc tttcagccgc ttggagacaa tatggccgcc gtaggtgatt acgagctcgg
     1921 gaactagctt ctctttttcc tcctccgggg tagcgtacag gatagcgtcg aaattcttga
     1981 tcggcaatcc cggtacgata cggttgccga tgtgctcgga gagccaagtg aaatgtttgt
     2041 atagtaactt acatattttc ttctcgaaga gatagatcaa actcatttgt cccgctatca
     2101 tcattcgctt gctatatttg ttcaaacgct cgatcagatc gtcatatttc cggtcgtaca
     2161 cgtttaggcc ttgataacgg gtgattaccc gtaccttcgg caattcgggt gtcgtgaact
     2221 gaaataaagg ctcggagatc gggacgttga tatgaaccgg ccctttgccg tgatggttca
     2281 gttccaacaa agcctcgttg atcaatcgat tacaatacca ttcatcttga tccgtatgta
     2341 tttccggtag ctgtaccgat ttcttcacca gcgagccgaa caccccgggt tgcggcaagg
     2401 tctgtccgtc catttgcccg atccatgcgg cggggcggtc ggcggatatc acgacaagcg
     2461 gaactttctg atagaaagcc tccgctaccg ccggatgtat attcagcaag gccgtgcccg
     2521 aggtacagca aacagccgcc ggcttacctc cgtgcaacgc tagccctacg gcaaagaatc
     2581 ccgcgcttcg ctcatccgtc acggaatggc aattgaagaa cgaatggttt gccaatgtgt
     2641 gcacaatcgg agagttccgg ctccccggac atagcacgat attacttacg ccgtgctcta
     2701 tcagcaaggc taccaattga aggatgttct ttttatcgct atacattata tgatataaaa
     2761 ttataaatta cccactattc tccgcatcgt atctaattta tcctctgtct cctgccactc
     2821 atcctctagc tgcgaggccg ccaacagacc gcctccggca tacagcgtaa aggtttgggg
     2881 gaggatattc atgcaacgga ggtttacgta cagatctgtt ttgcctttgg gatccagcca
     2941 accgatgaaa ccagagtaat agctacgatc gtacccctca ttctcgatga tgaaatgata
     3001 agcctcctcc ttgggaagac cgcaaacggc tggagtaggg tgtaatagtt gaaggacatc
     3061 acccagcttc tcgggattcg gcaaggagaa gaagaaatcg cttttcaaat gggaaacctc
     3121 tccggcccgg gctgaataag gccccttctc ctcgggagta atacccaagg tggacagctg
     3181 gcggcgtata taggaggcga ctaattgctg ttctctccaa ttcttatggt cccagctttt
     3241 cggtaatttc ccgtctcgta aggattgggt tccggccaaa gctaccgttt gccagtcgcc
     3301 tttctcgccg gataacaaga tttccggtgt cccacccatc caagttccgg tttccggggt
     3361 atggcaaagg tagacatagg agcggatata tcgttctact gcggcgaaga atgccttgcc
     3421 gggtgagaaa gagggatctt tccggatcgt tttgctacgg gataggacta gtttatcttg
     3481 gcttcctttt agcaaagggc tggtaaacag ggagaaatgg taagcgtatc gttccttctc
     3541 ctcggattcg gatgggattt cgatttcatt cgaagggaat tcgtttttct ctcgagagaa
     3601 atcccaatcg gattcggagg ggatttcaaa gcaatccggt tggattaaaa cgataggtcg
     3661 ttccgctgtc acctgaaacg gagcgattac aaaaccgctc cgttcattta agtcttttat
     3721 gtcgtataat aggcaaggag agccggcgct ttgcatcctg aaatggagcc tatcctcacc
     3781 gggaatacgc caaatagcga aactccggcc ttgcttgatc aacgtgtcta ttatttcgta
     3841 agaatctctt ggaatcattt cttctttagt atgctattga ttacccgtac cgaggaaacc
     3901 agtttgtcgg tagaggtgaa tatgtctacg ttccacacat gggatgaacg gcctttgtgg
     3961 ataatcgtcc cgacggcacg taccgtgtct ccctcgtggg cggacgacat atggctgccg
     4021 ctaacttgca tgccgactac catctcgtcc ggtttcgcca gaatcatgga acctagtccg
     4081 gccacggtct ctgccagagc cagcgtagcg cctccgtgca agataccgaa aggctggcgg
     4141 gtccgttcgt ctacaggcat ggtcgcttcc acacgatcct ccgacgcata ggtatattga
     4201 atgcctagat tacccattaa ggcatgtttg gcccgtgcgt ttagttgatc taacggaacc
     4261 tctgtgggtt tgatcgtggc aaggatcgct ttctcgatcg ctaccgccac accatccata
     4321 ttattagata aggtcgtgaa atcggcgcaa gcctttacgg agtcgcgtgc gttacccatc
     4381 gccacgccgg taccggctaa ttgaagcatg gatacgtcgg ctacgccatc accaatagcg
     4441 acaacctctt cggtcgtgat attcagcatc tccatcaata ccgccaacgt atttccctta
     4501 ttgatgaaat gaggagctac ctccaagaaa tagtcctcgg agcggaaagc ttccaatacc
     4561 ccgtccaaac gtttcttcca atgattttcg agacccacta gattgttctc gtcatcgctg
     4621 gtaagaatac acttacaggg tgcgaaatcc accgcttccg ggaaattctc cacaccgatg
     4681 atgcgcatct tattgagttc ggcctcctct tggacatgct tgttttccgg gctatcggtc
     4741 aggatatagt ccttgtgata cgtgaagata gcaaaaccgt tttccttcgc cttccggttc
     4801 aaataaggaa tcatggccgg atcgattcgt ttctcgaaca tcaattctcc ggtctgggca
     4861 ttgatgatct gacctccgtt ataagataat atgaaaccac cgtaatgatt caattcaaga
     4921 gcctccgcca agggttgaac cccgttggtg ggtcggccgg atgccaatac aacatgaact
     4981 cccatctgct gagcctttaa cagggcggca tgggtccggg gagttatctc tttcttgtcg
     5041 ttaagcaacg tcccatctac gtctaagact aataatttgt acttcatggc gtcttatttt
     5101 atagtttaga ataaaaatgt acggaaaatc tctggaatat acgccgaatt gca
//
