GCA_029984645.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaIBPKALHF_7 (original name was: gnl|Prokka|IBPKALHF_7)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS-like	71,615	114,044

gnlProkkaIBPKALHF_23 (original name was: gnl|Prokka|IBPKALHF_23)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	RRE-containing	9,688	30,041	polysaccharide C	Saccharide	20%

gnlProkkaIBPKALHF_43 (original name was: gnl|Prokka|IBPKALHF_43)

Region	Type	From	To	Most similar known cluster		Similarity
Region 43.1	phosphonate	1	13,063	polysaccharide B	Saccharide	30%

gnlProkkaIBPKALHF_66 (original name was: gnl|Prokka|IBPKALHF_66)

Region	Type	From	To	Most similar known cluster		Similarity
Region 66.1	phosphonate	5,985	15,951	polysaccharide B	Saccharide	13%

Region	Type	From	To	Most similar known cluster	Similarity
Region 7.1	NRPS-like	71,615	114,044
Region 23.1	RRE-containing	9,688	30,041	polysaccharide C	Saccharide	20%
Region 43.1	phosphonate	1	13,063	polysaccharide B	Saccharide	30%
Region 66.1	phosphonate	5,985	15,951	polysaccharide B	Saccharide	13%

Compact view

gnlProkkaIBPKALHF_7 - Region 1 - NRPS-like

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Location: 71,615 - 114,044 nt. (total: 42,430 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 71614 - 114044:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg7_93

AMP-binding (12..401): X

nrpys: Leu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALFLGVVLK
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (59% 8Å match); aIle DALVFGVVLK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaIBPKALHF_23 - Region 1 - RRE-containing

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Location: 9,688 - 30,041 nt. (total: 20,354 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaIBPKALHF_43 - Region 1 - phosphonate

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Location: 1 - 13,063 nt. (total: 13,063 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

Legend:

core biosynthetic genes

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaIBPKALHF_66 - Region 1 - phosphonate

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Location: 5,985 - 15,951 nt. (total: 9,967 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1